Comment: 0-22 hr AEL
TAGAGTTTTGCCGTCGCGTCGCGTTGTGAAAAGAGAAGAAAACCTTCTGAAAACAGTGCTAATAGATTAGGCTGCACCAG TTGCTGGATATCGCTATTGGATAAAGTCAAGAAAGGAGCTGCAATATGGCCTCGCCCTCTCCGGCGAACAGTCCCATGCC ACCGCCACAGGCGCCCAGTCCGATGGCCCCGCCCTCGCAGAGTCCCGCCCCCTCGCCGCACAGCCCGTATCCGCACCAGC AGCCGGGTCCACTGCAAGGACCTCCGCCACCCGGACATCCCGGTGCCTACGGACATCCCATGCAGCACGGACCACCCGGC CAGGGTCCACCCGGGCATCATATGCCACCGCACCACCAGGGGATGATTTTCTCAAAAGGTCCGCACATGGGAATGCAGAT GCCGCCGACGGGACCGAACATGTCCCCCTACCAAACCCACGGAATGCCACCCAATGCACCCACACAACCCTGCATAGTGT CACCTGGTGGCCCACCTGGAGGCCCGCCACCTCCGGAGCGCTCGAGCCAGGAGAACCTGCACGCGCTTCAACGCGCCATT GATTCAATGGAGGAAAAGGGCTTGCAAGAGGATCCTCGCTACTCGCAGCTGCTGGCAATGCGAGCAACCTCGAAGCATCA GCACCTTAATGGCAATCAGGTTAACTTGTTGCGCACACAGATCACCGCCTATCGGTTGCTGGCGCGGAACAAGCCCATAT CCATGCAGATGCAGCAGGCGTTGCAGGCCGCACAGCAACAGCCACCGCCAGGACCTCCAATTGGGCCACCTGGAGCACCA GGCGGACCGCCACCAGGGAGCCAGCATGCAGGACAACCGCCCGTGCCGCCGCAACAACAGCAACAACCGCCACCTTCAGC AGGAACTCCACCCCAGTGCTCCACGCCGCCCGCAAGCAATCCTTATGGACCGCCAGTTCCTGGCCAGAAAATGCAGGTAG CGCCACCACCGCCACACATGCAACAGGGACAGCCATTGCCACCACAACCGCCACAAGTGGGTGGACCCCCGCCTATACAA CAGCAGCAACCACCTCAGCAGCAGCAACAACAGTCGCAGCCCCCGCCACCGGAGCCACATCAGCACCAGTTGCCAAACGG AGGCAAACCCCTGAGTATGGGGCCATCTGGCGGGCAACCACTAATACCTTCATCACCCATGCAGCCACAAGTGAGAGGGA CACTGCCAGGAATGCCGCCAGGTAGTCAGGTTCCGCAACCAGGAGGAGGTCCTCAGCGACAGGTACCACCTGCGGGCATG CCGATGCCCAAGCCCAATCGCATTACAACGGTGGCCAAACCCGTCGGTCTGGATCCCATAACATTGCTGCAGGAAAGGGA GAATAGGATAGCAGCCAGGATATCATTGCGCATGCAGGAGTTGCAGCGACTGCCGGCCACGATGTCCGAGGATCTGCGTC TGCAAGCGGCTATCGAGTTGAGGGCCCTAAGGGTCTTGAACTTCCAGCGACAGCTGCGTATGGAGTTTGTTCAGTGCACG AGACGAGATACCACTTTGGAAACAGCGTTAAACATTAAGCTCTACAAGAGGACCAAGAGGCAGGGCCTGCGGGAGGCACG TGCCACCGAGAAGCTAGAGAAGCAACAGAAGCTAGAGGCTGAACGAAAGCGTCGCCAAAAGCATCTGGAGTTCTTGGCTG CTGTGCTTCAGCACGGCAAGGATCTAAGGGAGTTCCACAGAAATAATAAGGCCCAGCTTGCAAGGATGAACAAGGCCGTG ATGAATCACCACGCCAATGCCGAGCGTGAACAGAAGAAGGAACAAGAGCGCATCGAAAAGGAACGTATGCGACGTCTGAT GGCCGAGGATGAGGAGGGTTACCGCAAGCTGATTGATCAAAAGAAGGACAAGAGATTGGCTTTCCTACTGTCGCAGACAG ATGAGTATATTAGCAACCTAACTCAGATGGTGAAACAGCACAAGGACGACCAGATGAAGAAAAAGGAGGAGGAGGGCAAG CGGCTGATACAGTTCAAAAAGGAGCTGCTGATGAGCGGAGAGTACATTGGCATTGATGAGGGCAGCATTGTTGCCGATAT GCGGGTACATGTGGTGGAACAGTGCACGGGTAAGAAACTCACCGGCGATGATGCACCCATGCTGAAGCACTTGCACCGCT GGCTGAATATGCATCCTGGCTGGGATTGGATCGACGACGAGGAGGACAGCTGTGGGAGCAACGACGATCATAAACCCAAG GTGGAGGAGCAACCAACGGCAACAGAAGATGCCACCGACAAAGCGCAGGCGACGGGCAACGATGAGGATGCCAAGGATCT AATTACCAAGGCCAAGGTGGAGGACGATGAGTACAGGACGGAGGAACAGACGTACTACAGCATCGCTCATACCATTCATG AAAAGGTTGTGGAGCAGGCCAGCATCATGGTTAATGGTACGCTCAAGGAATACCAGATCAAAGGCTTGGAGTGGTTGGTT TCGCTGTACAATAACAATCTAAATGGTATTTTGGCCGATGAAATGGGTTTGGGTAAAACCATTCAAACCATTTCGCTGGT AACCTACCTTATGGATCGAAAGAAGGTTATGGGTCCTTACTTGATTATTGTACCACTCTCCACCCTGCCCAATTGGGTGC TGGAATTCGAGAAGTGGGCGCCAGCCGTGGGTGTAGTCAGCTACAAGGGCAGTCCGCAGGGAAGGCGATTGCTGCAAAAT CAAATGAGAGCAACCAAGTTCAATGTGCTGTTAACCACATATGAGTACGTGATTAAGGACAAGGCGGTGCTGGCCAAGAT CCAGTGGAAATACATGATTATCGACGAGGGTCACCGCATGAAGAACCATCACTGCAAACTCACCCAGGTGCTGAACACCC ACTACATTGCCCCCTATCGGTTACTTCTCACGGGTACACCTCTGCAAAACAAGCTTCCCGAGTTGTGGGCGCTACTTAAT TTCCTGCTGCCCTCGATCTTTAAGTCCTGCTCCACATTTGAACAGTGGTTCAATGCGCCATTTGCCACCACTGGCGAGAA GGTCGAGCTAAACGAGGAGGAGACCATCCTTATCATTCGTCGTCTGCACAAGGTTCTTCGTCCTTTCCTGTTGCGTCGCC TCAAAAAGGAGGTAGAGCACCAGCTGCCCGACAAGGTCGAGTACATCATCAAGTGCGACATGTCCGCGCTGCAACGCGTT CTCTACAAGCACATGCAGAGCAAGGGTGTCCTGCTCACCGATGGTTCCGAGAAGGGCAAGCACGGCAAGGGAGGCGCTAA GGCGCTGATGAACACGATCGTACAGCTGCGCAAGCTGTGCAACCATCCGTTTATGTTCCAGCACATAGAAGAGAAGTACT GCGATCACACTGGCGGCCACGGAGTTGTATCCGGACCGGATCTGTATCGTGTCTCTGGCAAATTCGAGCTGCTTGATCGC ATCCTGCCCAAGCTGAAGGCGACCAATCATCGCGTGCTGCTCTTCTGTCAGATGACCCAATGCATGACCATCATCGAAGA CTACCTGGGATGGCGCCAATTCGGCTACCTTCGACTCGACGGTACCACGAAGGCCGAGGATCGTGGCGAACTACTGCGAA AATTCAACGCCAAAGGCTCGGATGTATTTGTCTTTTTGCTATCTACCCGAGCTGGTGGTTTGGGTCTTAATCTGCAGACC GCCGATACTGTAGTAATCTTCGACTCCGATTGGAATCCCCATCAGGATCTGCAAGCCCAGGATCGTGCCCATCGTATTGG TCAACGTAACGAGGTGCGCGTCCTGCGACTAATGACAGTCAACTCGGTGGAAGAGCGCATCTTGGCAGCAGCCAGGTACA AGCTGAACATGGACGAGAAGGTCATCCAGGCTGGTATGTTCGATCAGAAGTCAACGGGTAGCGAGCGACAGCAGTTCCTA CAAACGATTCTGCACCAGGATGACAACGAAGAGGAGGAGGAAAATGAGGTGCCCGATGACGAAATGATCAACATGATGAT CGCCCGCAGCGAGGAGGAGATTGAAATATTTAAGCGCATGGATGCTGAGCGCAAGAAGGAGGATGAGGAGATCCATCCGG GTAGGGAACGTCTGATCGATGAGTCCGAGCTACCCGACTGGTTGACAAAGGATGATGACGAAGTAGAGCGTTTCCACTAT CAGTACGACGAGGATACCATTCTAGGTCGAGGCTCCCGGCAGCGAAAGGAAGTAGACTACACCGATAGTTTGACTGAAAA GGAGTGGCTGAAAGCCATCGACGATGGCGCTGAGTTCGACGAGGAAGAGGAGGAAGACGATTCGAAGCGCAAGCGACGAA AGCGTAAGAATCGGAAGGAAGAGTCCGACGATGATTCGTTGATCCTAAAACGGCGACGACGCCAAAATCTGGACAAGCGG TCCAAGAAGCAGATGCACAAGATTATGAGTGCGGTTATCAAGCACAACCAAGATGGACGAACCCTGTCCGAACCGTTCAT GAAGTTGCCTTCGCGGCAGAGGTTGCCCGACTACTACGAGATCATTAAGCGGCCAGTTGATATTAAGAAAATCCTGCAGC GCATTGAGGACTGCAAGTATGCCGATCTCAACGAGCTGGAGAAGGACTTTATGCAGCTATGCCAGAATGCACAGATCTAC AACGAAGAGGCGTCTCTGATTTACCTGGACTCGATTGCCCTGCAAAAGGTATTCGTTGGGGCCAGGCAGAGGATAACAGC TGCAGCGGATGCAGCCGCAGTGGCAGCCGGAGATAACACGGGTGAAGCGCACGGCAATGGAGGTTCGGATAATTCGGACA ACGACGACGACGATGGTGGCGATGACGGCTCCGACGACGAGGAGATTGCAACCACTTCAGCGGCGGCTGTCAAAATGAAG CTGAAACTGAACAAATCTCTGGCTAGTGCGCCCGCTACGCCCACGCAATCATCGTCGAATGTGAGCAGCGGAGCGGCTAC CACATCCAAGAAGCAGACGCGTCGCAAGCGCTCCCAAAAGAAGTACACTATTTCAGACGATGACGACGATGACATGGACT AGCTACCGTTGGGAGTGATCTTCAAGCGCGGATCGTCGGATGTCGAAGGCATGGGCAATTGATTATTAATTAGTTTAAGT TTTACTTTCAAGGACGATGCTTACCAAAACCCTAGCCTACGGGTTTTTTTAACCATCTAACCTATCATGCTATCACTTTT ATCTATACTAATTTTCTAGTTGTAGGTTCTCGATTAAGTTTCCTATTCAATTATTTTCGCACACACCATTTATTTCTCCC AATTCTATTGATTGTATAATTATGTGCTTTAAAATAAATGCATACCTAACATAAAGTAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
