Open Close
General Information
Symbol
Dmel\LD35730
Species
D. melanogaster
Name
LD35730
FlyBase ID
FBcl0164979
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
  • reverse transcriptase error
Library
LD_pOT2_cDNA
  • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    2,433
    NCBI
    Full Length sequence dataSequence Downloader
    AAAACAAGCAGCCACGCTAGAGAAGAAAATCTGGTTGAAAATTGGCAAAAATTCTAATTAAAAAATCGCAAAAGCCAAAA
    GAGAAGTTCGTAAGTCGTCCTACGAATTCCGCTGTGCGTCCCCACCACATTTTGATTTCTCCAAACGTGCCAGACACCGA
    GCAGGGGACTTTTTTCACGGAGCACCCCGAATTGCAGCGAAGTAATTTTCGAATATGGCCGAGGACTTTGATGTGGAGGC
    GATGCTCGAGGCGCCGTACATGAAAAACAATGTCAGTGCGGGCAGCGGTGGAAGTGGTGGACGCAAGCGCAGCGGCAGCA
    GTCCAGGCGGCGGCGGTGGACACGATGACGACTACGCCGAGAATGGTCCGCGGAACGGCAGCGGCGGCGGAAGCTCACAC
    AAAAAGCAAAGCAAACGATCCCGGAGTAGAAGCGGCTCGCGTGATGGCAAATCTCGACGAGATCGCGGCAATGAACGCAG
    CAGTCATCGGGACAAGGATAAGGAGCGCGATCGTAACCGCGACGGCGGCGACCGAGATCGACACCGCAGGGAAGGTGGCG
    ATCGAGATCGGGAGCGGGAACGTGAGCGCGAACGCGACAGATCTCGTCGGAGTCGTTCGCGTGATGAGCGCGGTGGAGGT
    GGTCGCTACGGCGATCGTGACAAGGATCGCCGCTCGCGAGATCGACGCGGCGGCAGCAAGTCGTTGCAAGTGGATCGCTC
    GCGGGACAAGCGTCGACGCAGTCGTTCCCGCGATCAGCAGCGCAAACGGCTGAGTCCGATCCGTGAACGCAAGCGCAGCC
    ATTCCCGCTCGAGGGACAGAAATAGCCGCCGTCGGGGAACCAATTCTCCGCGACGCCGCTCACCACCGAATGGAGCTGAT
    CGCACCACGCCCACCGAACTCAGTCCCGAGGAGCGGGACGCCCGCACCGTTTTCTGCATCCAGTTGTCGCAGCGAGTGCG
    AGCCCGTGACCTGGAGGAGTTTTTCTCCAGCGTCGGCAAGGTGCGCGACGTGCGTCTAATCACATGCAACAAGACGAAAC
    GCTTCAAGGGCATTGCCTACATTGAGTTCGATGATCCTGAGTCCGTGGCACTGGCCCTGGGCCTCTCTGGCCAGCGGCTG
    CTCGGTGTACCCATCATGGTGCAGCACACGCAGGCTGAGAAGAATCGTCTCCAGAATGCAGCGCCGGCATTCCAACCGAA
    GAGTCACACGGGTCCCATGCGCCTCTACGTGGGATCACTGCACTTCAACATTACCGAGGACATGCTGCGGGGCATATTCG
    AGCCCTTTGGCAAGATCGATGCCATTCAACTGATCATGGATACGGAGACGGGCCGATCCAAGGGCTACGGCTTTATCACG
    TACCACAATGCTGACGATGCCAAAAAGGCTCTGGAACAGCTGAACGGCTTTGAACTGGCCGGTCGGCTCATGAAAGTGGG
    CAATGTGACGGAGCGACTGGACATGAATACCACCTCGCTGGACACCGACGAGATGGATCGCACGGGCATCGATCTGGGTG
    CCACGGGACGTCTGCAGCTGATGTTCAAGCTGGCGGAAGGAGCCGGTTTGGCAGTGCCCCAGGCGGCTGCCAATGCGCTG
    CTGGCTACAGCACCTCAACCGGCGCCGTTGCAGCAGCAGGAGGTGGCACCATCGATAGCCACACAGTGCTTCATACTGTC
    CAATATGTTTGATCCGCGTACCGAGACAAATCCTACCTGGGACGTTGAGATCCGCGACGATGTGCTGGAGGAGTGCGCCA
    AACACGGCGGGGTGCTGCACATCCACGTGGACACCATTTCGCACACGGGAACCGTGTATGTCAAGTGCCCGAGCACAACG
    ACCGCAGTGCTGGCAGTGAATGCACTGCATGGACGCTGGTTTGCCGGTCGAGTGATCACGGCGGCCTACGTGCCCGTGAT
    CAACTATCACACCATGTTTCCGGACTCCATCAACGCCGTCGATCTGGTGACGTCCACGCGCAAGAACACCGACGATTGAG
    CTGAATGCGTGGGCACCATTTCGCACACGGGAACCGTGTATGTCAAGTGCCCGAGCACAACGACCGCAGTGCTGGCAGTG
    AATGCACTGCATGGACGCTGGTTTGCCGGTCGAGTGATCACGGCGGCCTACGTGCCCGTGATCAACTATCACACCATGTT
    TCCGGACTCCATCAACGCCGTCGATCTGGTGACGTCCACGCGCAAGAACACCGACGATTGAGCTGAATGCGTGGGCAAAA
    ATATTGTTAATATTCGTTACAAGAACTAATTTTTGGTCAAAACATTTTCAATTTTTTTAAAGACTCGACGCAAGCTGAAA
    ATTAGGGGCACATATTAAAATACAATTAAATACATTTATAAATATATATGAATTTGAAAATTTTAAAATAAAAAAAAAAA
    ATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    
    Library Information (1)
    Library: LD_pOT2_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

    Sample preparation

    Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

    Comment
    Reported As
    Symbol Synonyms
    LD_pOT2_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)