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General Information
Symbol
Dmel\LD23551
Species
D. melanogaster
Name
LD23551
FlyBase ID
FBcl0166520
Feature type
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    LD_pOT2_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      3,960
      NCBI
      Full Length sequence dataSequence Downloader
      CTGCCACACTGTCAAATTCCAGTAATTATTCTCGTCCACTGCTGTTGTTGAATTGCAAAGTCGCTCGGCGCGTTAGCATA
      GAAGCCCAGCATATATTTGCCCGATCCGAAAGCAGGCGGAGATCAGTGAAAACGTATACATATTTACCGCTAGTTGAGCA
      GTGCGAAAACGTGTCCGGAAAAGCCAAAGGCCAACGACAGCGAGTGCGAGCGAGAGAGCAGGTGACAGCGACACCGACAA
      GATTCCCCGCCTTCCCCCCTCCACCAACGAACCCTTTTCGACTGTTTTTTTGCACGGCACGGGGAAAACCGAAAGAAAAA
      CGAGATTTTCAATTGATTTGCGACGCAGTCCGGCCGGAAAATGCTCATAAACCTTAAGGTGAAGACCTTAGATGCGCGCA
      TCCACGAATTCAGCATCGACAATGAGCTCACCATCCGCCAGTTCAAGGACCAAATAGCCGAGAAGACTAACATTGCGGCG
      GAAAACCAGCGTATCATCTACCAGGGTCGCGTTCTGGTCGATGATAAGCAGGTGAAGGAATATGATGTGGATGGCAAGGT
      GCTCCATGTGGCCGAACGACCACCATTCTCGCAGCGCGGAGCCAATGCCCGCAACAATGATGAACCCATGCGAACCTTCC
      GCAATGTGGCACGTCCGCCGCCGCCCGGAATGCGTACTTCTCCGTACTTCCGCGCCCTCGATGGAATGCTGGTGGGCACC
      ATGGCCATACCGGTGAACAATGGTCCAGTAGCTGGGACACGTCCTCCACCGAATCGTTATCCCAACTCGTCGTCCTTCTG
      CATTAACCGCATCACGGTCGCCCTGCACATGATCGATTGCGCTGACAATATAGCAGCTTATCTGGAAAATCCTGCCGTTG
      GCCTGAACAATCAATCACTGGACATTCTGCAGCGCGGTCGCTGGTCGATGGAGTCCACAGTCGTGGAAGTAGGTGTCTCC
      TCGACTGATCTGCCACGCAACAATAACATCATCGATATGGTCCAAGATGCCGTGACCGCTGCTCTTTCGCATACAGGAGC
      ACGCAACTATACGGTGGTTCAGCTGCCCACGGTTTATACCAATGAGAATGGCGAGACGAGCCAGCAGAGAACAGCCGAAG
      CTATAACAAGTGAGGGAGCAGCCAGTGGAGCTGCAAGTAATGCTAGTGGTGAAACCACAGCTGCTACTGTGATTATCGAG
      GATGTTATCGAAACGGACGACGAAGTGGCCGATGGAGCATCAGATCGTTCGGTCACGCCAACACCAGAGCCGGAGGCGGA
      AGGAGCAGTTGGTGGCCAGCAAGTCACTGCAGCAGAGACTCCCACATCCTCGGTTGGATCAGCAAATGACGCGGCTGCCG
      AAGGCGGCAACAACTCAGGACCCAGGCGACGCACTCGTCCTCAGGTGTTGGCCCAAGTTATTCAGCACTATCGTGGCGTA
      CAGGCTCGCTTGGCGCCTTTTGTTGATCGGTACTATGAGATTCTCCAGAATGATCCCACTTTTGAGGAGAGTGACACCGA
      TGGTCGCGAGAACGCACAGCGCATCTTCGATCGCGTCTCGGAGGCTTTCCACTACCTTTCGCACGCCCAGCACGCTATAT
      CCGATTTAATGCTTGACCTGTCGCAGCCAGGGCCACGTGTGCTCACCTGCCGACCCATTCTCGTTGAGCAGAGCGGTTAC
      ATACGCTCCAATAACATCTTCACGCCCAACTTTTTGGCACCGCCCTCGGGTATTATCAACGAGCCTTTCCGCAATAGAGC
      ACCTGGTTCTGCCTCTGCAGCTGGCACTCAAACGGCCACCACAGCGGGCACTCAAACGCCCACCAGCGCGGGCACTCCAA
      CTGCTGTGGCACCTTCGCAGGCTGCCAATTTGCAGGCGGCCACCGATGCCAGTCGCAGTGCAGCGGCCATGGCGGAGATC
      GCCAGTCGAGCTGCTGGAGCCGCTGCGGAAATGGCTGCCGGAGCTGCCAGTGCGGCTGCTGGAGCTGCGAATGCGGCTGC
      CGCCGCCGCTCGCGATTTATCCAGTGATCAAGTAGAGGATCCCATCGACGAGCCTATGGTGGGGCCAAATGCTGCAGGTG
      CTGAAACACTAGCACAAGAGCAACAGCGTCTCGAAATGGATCCCCAATTGGAAATGGCCCGAATTATTCAGGCAATGGTC
      AATGGTCAACGACCGAACGATATCCATGTGGAATTCAATGCCCCCAACGTCATGTCGATTAACTTGCCCGTGCATGTGAT
      GACGACCGTGCGACAGGCTCCGGCAGCTGGATCGAACGAAACAGCAGATCAGTCTGCGTCCGAATCCTCCTCCCCTGAAA
      GTGCGCCTGCGTCGGGCAATGGAGAGTCTCCAAATGCAGCTTCAACATCAAGTGGAACACGCAGTGGCGATCAGAGGGCC
      AATACCTTGCCCACCACGGCCACCCAAACGCGCTCGACATCAAGACCACAGATTCAGATTGGCGGAAACAACAACTGGGG
      CGGACGCATTGCTCCGACACACACGGCATTCGACCGCTTTCTGCCTTGCAATAGTCATCACATTCGGGAACCAGAGCAGC
      TGCTCCAAAACAACAGTACCAGTCGCAGCACCTCGACAGCACCAGCAGGAGGAGCCACCGTTGTTCCGCCCACATCTGCT
      GCAGTAACTCGTCCTGCTTCGGTAGGCAGTGCAGTTGCCGCCGTCGGAGATACGGCCGCCTCACCATCAGGAGAAGCTGC
      CCCAATTGCTTCAACCACGCCAGCTGCTCCGCTACAAACTGTTTTGCCGACATCCACACCCACTCCTCGCGGAGACTCGA
      ACAACCTGCGTTCGCAACTGCGCACCTTCCTCAACGACAGCCTGTTTGTCGGAGTACCCATCAACGAGCAGACTATTCCG
      GGGGCCATTGGTCGCGCACTAGACTGGTTCGGGGAGAGCCTGGTCTACCTGCCGCAGTACGAGCGTCCGGAGTACAACTC
      GCGCGACTCTGTGTGCAACATCCTGCGTGTCAGCCTCCGTTTGATCATCGAGCTTTGTAATGGAGCTCCGGCCGGTGTAG
      ATAGTGCTCAATTCGAGCAAAGTCTCAAACAGATTTGTGACCAATTCCGCAAGCGGCTATACAGCGTTCTCTTCCTGTGC
      CTTGGAAGCGCGAATGCGGAGCTCTATTGGCGCCAGCTAATGCGCCTCCTTTGCGCACCGATGCGATCCAACTTCCGCAA
      CGAAGCCCTGCAGTTTTTGTGCATCTACATAGACCCCACGATTCCCGCCCAAACTGACACAGTGGATGCCCAACAGTTCC
      TGGTCTTGCGCAGCATTCAAGCAGCTCCTCCAACAGCTGCCGACGAGCAGCAGCTGCAAGAGCAAACTCTGGACACGGAT
      GTTGAGATGTCCGAAGTGACCGCCAGCGGCAGCAACAGTTCACCGGCAGACGAGCTACCAGCTGTGATTGTGGGCTCAGA
      GCCTTGGCACATGAGTTTCCCCAATGATTGGTTGCCTGTGATAACGCGCGACCTACAGACCCAGGCAGAGCAGAGTAATC
      GTCCCCAGCCGCCCTTCTCGGATGCCTACATCTCGGGCATGTCCGCCAAGCGCCGCAAGATAATTCAGTCGGAAAAGCCG
      ACTGCCAGCGTGGAGTGTCTCATTGCTAATGGAGTGCAGAGGGCTATCCAGAGCGTCGGCTTGGGTGGAAGCAATGGTGG
      TAGCGCCTTAAACGCGTCGATCAGTATGGATACCGTAATCGGATCCATTGCTCACGACTCCACCATTCAGGCCGCCTACA
      CGGATGCGGTGCGCAATAGTTTAAAAGAGCGTACCGAGCGGGATGTGGATTTTAAGACCAGCAAGTATCCGCAGATTGCC
      AAGTTCACCGAGCAAAAGTAGGGGGCAGATACGGAGACGGAAGCCTGGCCACCTAATGCTTCTTGTACATTTTACTTTAA
      TAAATTGTATTTAATTTAGTTAAAAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Library: LD_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

      Sample preparation

      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      Comment
      Reported As
      Symbol Synonyms
      LD_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)