Clone Dmel\LD29482

General Information
Symbol	Dmel\LD29482	Species	D. melanogaster
Name	LD29482	FlyBase ID	FBcl0166834
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\RhoGAP19D

Collection Status	BDGP
Known Problems	none
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	5093
GenBank	AY058603
Full Length sequence data	TCTTTGAGCGCAAGCTGGTGTCACACATGTCCAAGGGATTCGATCCGTTT AAGCCGCTGTCGATCAACACCAGTGCCCTGGAGTCGTCTGCCAGCACGTC GAGCACTTCGGCCACCACGACGGTGAAGAAGCAAAGTGTGCTGTATGAAT CCCTGCAGCAGCATCCATTGGCCAAGTACCATCAGACGCAGCAGCAAACG ACAACGACGACGACGGTGGATGGACAGACGAGTAGCCGGATGGAGCTGCA CAAGGCGACCCTGGCGAATGCCACCATCGATCCGGTGCCAGTTGGATCCA GCACAGCAGCCATTGTGGACAGTGCGTCGGGAAGCGGCAACGTTGTGCGG CGCTACAAGCCATTATCCTCCAGCTCCTTTGACGAAGGCAAACCAATGCG TAGGATATCCTACCTGCGGGCAACCAACAATGAGACCGACTTCAATGCGG AGGCTGCCGCTGGCGGAGATGCCGCGGCAACAGGGGCGTCAGGACCAGCA TCTGCATCAGCAGCTTCATCTGCACCTGGACCCACGCCCTCATCTGCTCC TGCACCAGCTCCAGCACCAGCACCCCTGCCCATTTCGATCCCCATTCCGG TGGCTGCTGTCGTCGAGCCGCAAAAGACGAAGTCCCTGGTGGAGGAGCAT CCGGATCCCACCTACGATGTGATCGGAGGCACAGGTGGTGGCCACGAGTT CATCGAGACGCCGAACGGCCTGGAGGATGTGCGTCTGTCGGCCCACGGGA GTCGGAGGGCCTCCATGAGCAGTGACATGCGAAGCAGCATCCACATTGAT GCCGAGTTAAATGGCAAGTATGTGCCAAACGAACTGGAGGCCTTTGAGAC GGAAATCGAGATCAAGTCCTCTTGCATCAACGGAAAACGCATGTCCGAGT ACCGATCATGGCGTCAGGTGAAGCTGGAGATCAAGGGAGATCTGCTGCGT ATCTACTCAGGCCGACATGCCAAATCGGAGCACAATGTGATAGAACTTGA TATTAGAAACTTTAAGTTCTTCGACGAGTCGATGGATAAGAAGAAATACT TGATCCGTATGCAGTCGAAGCCATCGCCCAGTGGAGCCCATTTGGCCTCC TTGGGCAACGAGTTAAACCTGGACGAGGGTCACGACAAGCTGCTAACCTC GTCGGGCAGCAGCAGTGCGAATGAGCCCGCTGCCAACGCAGCATCGGCCC TGACCGCATCCACCTCCTCGTCCACCTGCAGCAGCGGTCCTTACACGGAG ATCCTATTCAAGACGAAGAGCAGCAACGAGATGAAGCGTCTGTTTGGACT TCTGCAGTGGAAGGATAGCCTCAACTACGATGATAATGAGCACCAGCCAC AGGGCAAGCAGTTGGCAGAACCACAGAAAACAGTGGCCAGCGCCAGTTAT CTGGACGACGTCCAAGGTGGAGCGAGTGCAGCCGACAGCTCACCCTTGAG CTCCCATTCCGGTGGCATTGCAGACGCTAGTGGCAGTCATCATGTTTCTG CGCTACCAGCAGCTGCGGCTATTGTCGCCGCCACCAGCGACTCCATTTCG CCGGTGATGAAGGCAAGGAAGTCCTCTTCGCACAAGCATATACCTGATAA GGATTTGGGATCGCCCAAATCGAAGAACTGGAAGGATTTGCTCTTCCGCC GTGGCGGCAGTGGCAGCGGAGGAGTGTCACACCACGACTTGGCTTCGCCA TCCGCCTGTGCAGCCAAGCAGATTGGATCTATTGGCGTTCCACTGCGCAG CTGTCCCATGTCGAAGGTCAATGCATATGTGCCGCATCTGGTAGAGGTGT GCACGAACATTGTGGAGACAAAGGGTCTGGGAGTGGTGGGCATCTATCGC ATACCCGGCAACAAGGCGGCCATATCAGAGCTGTCCGAACTTGTTAACAC CAAGGACTTTCAGTTCGAGAGCTGTGCCAGCGATGACCGCTGGGAGGATG TGAATGTGGTGAGCAGCCTGCTCAAGTTGTTTATACGCAGCTTGCCCGAT GCCTTAATGCCGGCAAGTTACTATATCAACTTTATTGAGGCGGACAAGAA GTTTGGCCTGGAGAGAATTGTACTGTTGCGCGAGATTGTGGAATCGTTAC CACGGCATCCCTACGAAACGATGAAGCACTTGATTCGGCATCTGTGCCGA GTTAGCGGCAATTGCGATGTGAACCGCATGGAGCCCAAGAACCTGGCCAT CATATTTGGGCCATCTATAATCAGGACGCCGAACGATACTCTGGAAACGG CAGTCAAGGACATGAAGCATCAATGCCGCATCGTTGAGCTGCTGGTCACA CAGTACGACTACTTCTTCGAAGGCGGCAGTCTGCCGGATATAGCCGAAGT AAGCGGCGGTACAGCTGCCGTAAGCGCCGCTCAGCCGCAGCAGCAGACGC AGGAGGATCAGACGAGCATGCTGCTGCACAACCTGTCGAAGATCGAGCGG ATCACAGAGCGCGAAACCACGCGCACCTCGCGCTTTATGCCGCAGCTCAG ACGAAAGACCCATGGCAAGCGCAGCGCCGTAAACTCGGACACGTACTCCG GCGAGAGCGTTCTGGTAAGCGGGAGCTCCTCCAACACCACCACCACCACC ACCACCACCACCCACACCACAAGTAGCACAAGTAGTAGCAACACCATCAC CACAACCATCAGCACCACCACCATCCAGCAGCGACGGGCGCAGCGCAAGG CTGTGCAGAGCATTTGCGACCAGGCTCTAATCCTGCTCCTGCCCACACCC GTTTCTTCCGTCTCTCTTCCCTTTCAGTCGTTGGACTACGCCAAGGCGTC CGCCTCTGCCTCGGCATCCGCAAGCAGCACCGCCAGTACTTCGAGCAGCT CGACGCTTCACAGCGGGGGCAAGGCATCCGCCTCATCCTCATCCAGTTCC ACTACCAAGTTCAGCTCATCGTCATCGTCGGCGTGCTCAACAACGGCGGT GGCTATTTCTTCCTCCATCTCGGCGACGTCCTCCAAATTGTTAAGCGCCA ATTTTAAGAAGCGTAGCACAGGAGGTTTCTTGGGCTCAAGATCCTCGCAC CAGACGCGCAAGGCCAGCTTGGACGAAAAGGACTCAGGACAGTCGTCTGG CAGCCTTGGTCACAGCCTGGGAGTAGGCATAAATCTGGCCAAGGAACAAC AGCGCAGCAGCGTGGACATATCCATTCTGCTAACGGACGACGACTCTAGC AGCAGGCTAAGCGATACGGGTTCCATGTCGCTGACGACCATTACGGATAC ACTGGACAGCAAGCTACGCAACTTGCGCAGCGGCTCCGAATCGAATGATG AGAATGGATCGCCCGAGTTTCCCAAGAACCGGCGGCACACCTTGGGCCAG CCTCTGCACATGCACTCCGAGAACATTCCGTATGCGGACGAGTCGCCCGA GCGGCTGTTCCATCAGTTTTGCAATCCCCTGGAGACAGCCCCCTTATCCC TGCATCTAAGCCGCTCCTGCACGGCCACAAATACAATTGGTGGCGATGAA AAGCCTTCGAAACAGTCGGGTGGAGTTGCACCACTGCCCCCAAGTTTGCT TAAGGCCGCACATAAGCTACAGCACTCAGCCACGGTGCCCATTCATCAGG GAAGCTCCACTGCACCAACTAGCGGAGCAGCCACGCCAGTAGGCACGCCA ACCGGAAGCGGTGGGGCCGGAGCTATTCCCTCGCGCAGCTCATCGAGCAG TGTCACGACGCTAACCAAAAGCATACAACCCAGGTTCAGTAACTATCTAA GCTACGAAAGAGGCAATGCAGCCGCTGGAGGCGCCGAAAGGGTGGTGGGA TCCGCCGGAGGAGCTGCCTCCGACGACGATTCCGAGGGCTCGACCACCAG TGATCCCAAGGAGAAGCTGCTTCTCGGTGAACGACCGTCGCCTTCGTTTT TTATCGAGCGCTACAACAAAAAGAGACGCGATCATCGGTTATTTCGCAGC GCCAGTTTCAATTGCCGGAACTACTCCACCAGGCACATAACGGCGCAGAC ATCCGGAGGAGTAGCGGCGGGAGTATCGGGCGGAGTAGCTGGAGCGGTGG CGGTGGGGGTGGCTTGCTGCCAGGCATTGAGCACCACTGGCTTGACCAAG GACGAGAAGACTGATATGAACCTGACCAAGAAGCGCCAGATCCAGAACAA GCAGAATCGGTCCATTAAGCGGCGGCACACAGTAGGCGGACCTCACGACT ATTCCGCCAGCAATGGTGGCTGCTCGAATCACTCACACGGTCACGACCTG GCAGCCATCAACTACAATCACCACAATCGCCATCACCAGCAACAGTTGTT GAAACTGGGCAGCGCTACATCTGGCGGTGGAGGCAGTGGCGCAGCCGCGG CCGAGACGACGACCACCACAACGACCACCACCACGGTGCTGCGGCGCCTC GGTACAGGTCAAGCTGGCGCCGAAGCTAGTGTGGCATTGCCCGCATCAAG TTCCGGATCGAGCAGCAACAACAATAACTCGCCCCAAAGCGATGGCAGTA ATGGCAACGGCGGTGGAGTGGCGGGAGTTGCCTCCAATGCACCACCAACT GGCGTAGGTGTCGGTGGCAACAACAGCAGCGGAAGCAGCAGCAACAGCAG CAGCACCAGCACAACTCCCGCTGGGAACGGCGATCAGGTCCTGGAAGTGG GCATACGTATCAAGAACATTAGCCGGGGTCGCTTCTAAAAACCTGTATAC CCAGACTAAGAAAACAGACGGTGGCAATTTCACATGGTGAAACACACATG AAACACTCTGGGCGAACTCACATTCTTAGCAAACAGTCTATACAAAGCTA AAAGTGCTAATATGGCTAAAAGCAGGCAGTCTGCTTATATCGCGTTTTAA AGAACCTGACAAAAAACATAGTTAAAAATAGCTCCTAGTGCAGATTGCTG CGACAAGACCAACCCGAAATAGCAGTTGCTTAAATGTGGTTAACCTGATT AATTGACACTTTGAAACCCACAGTTTACAAGAGTATTAACGAAAGGTTTA ATATATAACCAAAAGGCATAATGAGAAGATATTTAACAATAGTCTGTTAA ACACACAAAAGCTAAACACACCTTTAAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0166834
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]