Clone Dmel\LD25711

General Information
Symbol	Dmel\LD25711	Species	D. melanogaster
Name	LD25711	FlyBase ID	FBcl0167113
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)

Collection Status	DGC gold BDGP
Known Problems	contains transposon sequences
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	2209
GenBank	AY061332
Full Length sequence data	CTGCCCGAACGCGTCTCCCGTGTGCTTCAATTCTCAGTTTTCGATACGAA TGGTCGCCATGGTCTAGTCCCCATTCCATGCCATTCCATTTCCATTTCCA CTGGCCTCGTGTAATTATGATATTTTCCAGGGTGTTACGATTAAAATTAT TGATTTGTGTGGCCCCAGAAACACTCCCCCGCGCAGAAAAGTGATAAGAA GTGGACGCGTTGCTCATGCTTTTGGCTTTCTTCCGACGTTTTCACGTTTC GTTTAAGTGCACTGAGCAAAAAAAGTGCAATCTTTGACAAGGCATTTAAT ATATGTACACTCTTTCACTGGATCCTACACACTTGCTCGTACATGAATAT GAAATAAACTGTGACGAGACTTACCTATAAACGATTTGTTCCACTAAAAA TGCAAAAAAAAACCTAATTTGTACAAACCCAAAAACGTGACGCCCACAAC CAACCCAATTTCTTTTCAGTGAATCCCTCCCACACAACACAAAAACGCCA TAAAAACAAAAGAGTTAACAAAACGCAACCATAAACCAATGAGTTTAAAG TGAAAGAGTGAAGAATTAGGAAAGAGCAGAATCGTGCAGATTTCGAGTGT GTGATATCTGTTTTGTTTTTTTTTATTAGTTTTTTTTTGCACAGTGTGAT CATGGCCGGCGATACGGAGAACAAAAGGGGCTATCGCACCATATTTCGCA GCATATCCCAGGTGTTCTACGCCAATGCAAAAAACTCGAGTAATACGAAC AGCAGCAGCAGTAACAACAACAATAATATCATCAACTACAACAACAACAC CATCGAGAACAACAACGAGGGAATCGGCCAACTAACAGTTACGCTCACCA GCATAACACCACCCACAGCCACATGGACGTCGACATCGCCCACAATCTCC ACGTGCACAACTGCGTCCGCATCGGCGGGATCATCCACCGCATCCTTGTC GCCACTGGCTAGCAGATCAGCGCGATCGGCGGAGAATATATCCTGCAGCG AGTGTGGAAACCCAGATCCGTATCATGGCCAGGACATGGAGTTGGCAAAA GGTCAAGCCCTGACTCCCGAAGTGCCAGCAGTGCCGGTTCCAGCTGCCAG TGCGGATCAAAACCAGAAGCTAAGGGCCAGTTCCATGCTGGATCTGACCT CCAACCATCGCCATCAGAACGGCTTGAGGATTGTGCCAGTGACTGACATT CTGCGGGCGCAGCAGATCCAGGAGGACAATGAGGTGCAGCAGACGCAGCA GTCAACGATTCTCCAGCGCTATAAGAGCGTCTCCATTGGCAACCTGCTCG ATCTGCCCGGCGAGGATGACAAGAAGTCCATCAAGAATCGCATGCGAATG AAGTTCGCCGTCAATAGCAATGTGTTTCGCATCAACCGTTCGCCGAAGAG CGAGAAGAAGTCGCGTTCACGTCGAGGCCAGGTGAACAGTTTGTACCTGG ACGAGCAGAAGTATCCGCTCTTTGCCGCTCCGCTCAGCGCACTGGAGCTC AACATGACCGATCATCCGAATGTTCCACGTTTCGTGGTCGACGTTTGCGC CTACATCGAACAGCCGGAGTGCATCGAGCAGGACGGACTGTACCGCGCAT CGGGCAACAAGGTTCTGGTGGACGAGCTGCGCAAGAAGCTGACGCACGTG TACGATCCCCGCTGGCTGAAGACGGACGATATCCACACGCTGACCAGTCT GCACAAGCAGTTCTTCCGAGAGCTCACCTCTCCGCTCATCACCCAGGAGG CCTACGAGCGACTGGGCAGGAGCCTTAACGACGACGCCGCCATCGAGCGG ATGAGTTTGGCCTTTGACGACATGCCGGAGCCGAACCGCTCCACGCTGCG CTTCCTCATTAGGCACTTGACCAGAGTTGCCGCTGCCAGCGCTTCGAATC GCATGCCGTCCACCAATTTGGCCATCGTTTGGGGTCCTTGTCTGCTGAGT GCCAATCAGATACAGCTGGACATTGGACGCATGAACATGCTGGCCAAGGT GCTGATCGAGAACTATGATCGCATCTTTCATCCGGACAATGAGCGTCTAG TTTGTTAGGATGCAGGCTACCCGCCCTTCGCTGCCACGCCCCCTTCTCCG CACATCACAAGATCGAGCAGCATCGATATACAAGTGCCTCAAACATCAAA CATCACCCCTCTCACCATATGTTTTGTAATGATGAACTCCAAAAAAAAAA AAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]