Clone Dmel\LD27836

General Information
Symbol	Dmel\LD27836	Species	D. melanogaster
Name	LD27836	FlyBase ID	FBcl0168316
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)

Collection Status	BDGP
Known Problems	none
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	3049
GenBank	AY118541
Full Length sequence data	AACAGCAGGATGCTAAGGAGCGTTTCAGCTACCAGGGAACCTTTACGCAG CCAGGACAGAAACAGGAATCTTCAGCTCCAAGACCTGTGCATACCAACAA CGGTGCTACTCTACGAAAACCTCGAGTGGAACCCGTCGAAGATGGTCAGA TCAAGCTGCATGTGCCTACGCCTACAACACCTGCCCGCAGTCCCCGGTAC TCCTTGTTGTTGTGCGACACCGAAAGCTCCGATAACAGTTCGGCGGTAAA TACACCGCAATATGACATGGAGCCCCTTATGCTGACATCGGCCATGTCTG GCGTATCCGGTGTTTCATCCAATGTACAGCAGCAGCAGCTGCTTTTAGGT GTGGATGCGTCCAGCAGTTACCTAGGCAGCTCGCACGAGAGCTTGGGACA GAACATCAACAACCACCATGATGCTGAGCAGCGTGATATGAATGCCCTAA AAAGGGAGCTATCCTTGGACCTTAACCCACTGCAGCCGCGCTTACCACAG CCCGCAAATCGAGCTGCCACTTTACCGGTTAAAGATCAGCTGCAGGCTGC TGCAGCGGCCATGTGCTCATCGCCCAACAATTCCAATTTTACGGACAATA CTAGTCAGTCAGTGACACCGAGCGAGTATGGTTATCAGCACTTGCAGCGA CAGCTAAGCATGCACTCTCTGCTGGCGACTGATGAGAGTTCGCCCGTGTA CGAGGACTTCGAACAGACCCCCGTCAAGATGGTGCCCAGCCCCATAAAAT CCACCATTAGCATCACATACAAGTCGCCCGAGAAGGAAAAGAAACCTTCG GCCATCTTAGAGACCAACTTCGATGAAAACACTGTATATGAGCAGGTGAA GCTGTTCCGGAACTCGGTAACCGAGGTTAATCAAATGTTACATGAGCGTT CAAGCCTGAAACAGATTGCCGAGGAGGAGCAGCACGATGGAGGTGCTTAC AAGGCAGCAGAGATGACGCAGCAACTGCTGCAGCTTGAACAGGAGCACCA CGACCACGAAGAGCAGCAGGAGAAAGAGCCTGAGGATGAGGAGCAATCCC AGCTACTTTACGAAAACATTGAGCTACGCAAACCGAAAACAGTTTACGAA AATCTACGTGGCGAGGAGATGAAATTCATTACAGAAGAACAGAAACCCAA CGTTGATAGAATTGAGCTTGATAGTTTAGACAGCTTGCCAGACAACATGG AACACGAACAGTCGTCGCCCAGCAAATCGCCCACTTTCAGTGTCAAGGAG TTGGCCAACAAATTCGAGAGCTCGCCTGTGGAGCAGCTGCCCAACTTTGA TTTTTCGGTGCGTGGCGGCTCGATGAAGAAACCTAACGAACTGAGCGTAC CCAAGACGATGCCCGCACCCGTGCCACTCAAGAAACTGAACAGCAGTGCC CAAAAAATAACCCGCTCGTTGGATGAGAACGCCTTCGTCAGAGAGTTTGG GGGCAAGCAACTACAAGATCTGTCTCTCTCTAACAAACTGCCCGAAGTAA TGTCAGAGGTCAACAGCCGGCGCAAGAGCTTTGACTTCACGCGACCTAAA ACCTTGAATCCACCAAAGCGGTTGCCTGGTATGAGCATTACCGAGGAGAT ATGCCAGAAGCGTGTTGGCGAAATGGAGGCAATTACCCCCACCACCGAGA ACCGCATCTCACTCATCCAGCAGAATAATATGCCCAAAGAGCAGCAATCG TCGGCTAATCAGTTCCTGCCCCCGGCGGGTCGAAAGAACGTATTAACAGG CGTGGTCTTGGATCGTGAGCGCATCGACAAAATCAAGGAGGAGCGGCGTC AGCAGCTAACGCAAAAGTACTACGGCGATACTCTCAAGTCTCGGTCGCGT ACGGAACTGAACTCTGAAGACAACTTCCCGGCTGCAGAATCCCTGCGAAT CAAGTCCAAGTCACGCGGCGATATGCATGCCTTGCAAAAGGATATCGACA TGAACCTAAAGCAACTGAGCCGCGTCACTGGCGGCGGATCGGAGTGCACC CTCCACACGGGCTTGAGCCAGGGTAACGGACAGGAGCATCTGGGACAACA GCGCGTGCGCCGGATTTCCGACGAAAAGAACCAAAATTGTGATACCAGTA ATGGTGGCGTAAGTGGCATAACCTCCACTTCGCTACTCAGCGTCAAGACG ACGGCCCAGAAATATGGAAGCACCAGCAAGACGCCAGCGAATAAATTTGA ACGTAGTCAGCCCATGCCACGCGATCGCTTGCAGAGGAACTCCTTGGCCG AGAGCAATGCAGGCACCAAAGAAAAGATCTCACCACAATTCTCAATACGC GACGTGACAGCGATGTTCGAATCTCGTTCACAAAATCAATAGGCTAAGGC AACTTTAGCAACTAATTACATCGAAAAAATGAGACCGCTACCTACATATA ATCACAGTTCTGGAGCAGCGTGTATATATGCCAATGAACGCATATATATT TTATTTAGTTTTTAATCATTCACCTAAGCGATTTGAAATTCTCAAGTGTT ATTGACATATACGGCGCAAGCGGATCCAAAGACATACTGATTTATAACGC CGTGTTTATTGCAAACACATTTATAATGATATAACGATGATAATCATAAT GACGACGCTTTGCGCCTTAAATCATATGCAAAATTCAATTTTGTTTTGAA GCAGCATGAAGAGAGTTTAACTAAAAGACCATCCTTACCATACCCCTCGA TCCTGAACAATGCATATTGTTATTGCATTTTGGGAGTTTCTTTAAATAGG TGTTAAGTTTCATTCGTTTAACCATACAAAAAAACGGAATTCAACCCTCT TGAATTCCATTTCGATTTCGATTTCGATTTGTTTTTGCCTGATTTGTGTC TCGAGTGTTTTTATATTCTTGTTTTTGCGACTAAACTTTGACGCATGTAT CGGAGATGATACTTTTCTTAGTTTCTAATGAAAGCATTTGCCACAACTTT GAGCAAGGTGTCCTAAGTTAATTATTATACGATTAATTTATATGTACTTT TAAAACAATAAACTCAATTGCATTGTCACTCAAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]