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FB2025_05
,
released December 11, 2025
Comment: 0-22 hr AEL
TCGCGTCCAAAAAGTGTACAATTTTTCACCACCTCGGTTTGTTTTTTCGGACCAGGCGAGGCACCTTCGATTCGCTGCGA CTTCCGTTTCCGATCCGTTTCGGATGATAGCTCTGAGTGCGCTGCTAACCAAATATACGATCGGTATTATGAGCAACCTA AGCAATGGCAACAGCAACAACAACAACCAGCAGCAGCAGCAACAACAACAGGGTCAGAACCCACAACAACCAGCACAGAA TGAAGGCGGAGCGGGAGCGGAGTTCGTGGCGCCACCGCCCGGACTAGGTGCCGCAGTGGGCGTGGCAGCCATGCAGCAGC GCAACCGCCTGCTGCAGCAGCAACAGCAGCAGCACCACCACCATCAGAATCCCGCCGCCGAGGGAAGTGGCCTGGAGCGG GGCAGCTGCCTTCTGCGCTACGCCAGCCAGAACTCCCTGGACGAGAGCTCCCAGAAGCACGTCCAGCGGCCAAATGGCAA GGAGCGCGGCACTGTGGGGCAGTACAGCAATGAACAGCATACGGCGCGCTCCTTCGACGCCATGAACGAGATGAGAAAAC AAAAACAGCTCTGTGATGTGATATTGGTGGCCGACGATGTGGAGATCCACGCTCACCGCATGGTGCTGGCCTCCTGCAGT CCGTACTTCTATGCGATGTTCACCAGTTTCGAGGAGTCGCGGCAGGCGAGGATCACCCTCCAGAGTGTCGATGCTCGGGC CTTGGAGCTGCTCATCGACTACGTATACACTGCCACGGTGGAGGTAAACGAGGATAACGTGCAAGTCCTCTTGACCGCCG CCAACCTGCTCCAGCTCACGGATGTGCGAGATGCCTGCTGCGACTTCCTGCAAACTCAGTTGGATGCCAGTAATTGCCTC GGCATCCGGGAGTTTGCCGATATTCATGCCTGCGTGGAACTTCTCAATTACGCAGAGACCTACATCGAGCAGCATTTCAA CGAGGTGATCCAGTTCGATGAGTTTCTGAACCTCTCGCACGAGCAGGTCATCAGCCTGATTGGCAACGACCGGATTTCGG TTCCAAATGAGGAGCGCGTATACGAGTGCGTCATCGCCTGGCTGCGCTACGATGTTCCTATGCGGGAACAGTTCACCTCG TTGCTCATGGAGCATGTGCGTCTGCCGTTCCTGTCCAAGGAGTACATCACACAACGTGTGGACAAGGAGATCCTCCTCGA GGGCAATATCGTGTGCAAGAATCTCATCATTGAGGCATTGACCTATCATCTACTGCCCACTGAGACAAAGTCCGCTAGAA CGGTGCCCAGGAAGCCGGTGGGCATGCCCAAGATACTGCTCGTTATTGGAGGACAAGCACCCAAGGCTATACGATCGGTG GAGTGGTATGATCTGCGAGAGGAAAAGTGGTACCAGGCCGCCGAAATGCCCAACCGCAGATGCCGCTCCGGCTTAAGTGT CCTGGGTGACAAAGTCTATGCTGTCGGAGGTTTCAATGGATCACTGCGTGTGCGCACCGTAGATGTCTACGATCCTGCCA CGGATCAGTGGGCAAACTGCAGCAACATGGAGGCCAGACGATCTACCCTGGGAGTAGCTGTCCTCAATGGTTGCATATAC GCCGTGGGTGGATTCGATGGCACTACGGGTCTGTCGAGTGCCGAGATGTATGATCCCAAGACTGACATCTGGCGGTTTAT CGCCTCCATGTCCACGCGTCGCAGTTCTGTGGGCGTAGGCGTCGTCCACGGACTTCTTTATGCCGTGGGCGGTTACGATG GCTTCACTCGTCAGTGCTTATCTTCAGTGGAGCGCTATAATCCGGACACGGACACATGGGTTAATGTGGCGGAAATGAGT TCGCGGAGGAGTGGCGCCGGAGTGGGAGTGCTGAACAATATCCTTTATGCCGTTGGCGGTCACGATGGTCCCATGGTCAG AAGATCTGTTGAAGCCTACGATTGTGAAACGAACTCCTGGCGTTCAGTGGCAGACATGTCATATTGCCGGCGAAATGCGG GAGTGGTGGCTCATGATGGCCTGCTGTATGTGGTCGGTGGCGATGATGGCACCTCAAATCTAGCTTCTGTGGAGGTCTAC TGCCCGGACTCGGACAGTTGGAGAATACTGCCTGCTTTGATGACCATCGGACGCAGCTATGCGGGCGTGTGTATGATCGA CAAGCCCATGTGAATGGAAGAGCAGGGTGCATTGGCCCGCCAAGCTGCTTCGTTGGCCATCGCACTGCTGGATGATGAGA ATAGCCAGGCGGAGGGCACGATGGAGGGCGCCATTGGTGGTGCCATCTACGGTAATCTGGCGCCGGCTGGTGGGGCTGCA GCGGCTGCAGCTCCAGCTGCGCCGGCTCAAGCTCCCCAGCCAAATCATCCGCACTACGAGAACATCTACGCGCCAATTGG ACAGCCCAGCAACAACAACAATAACAGCGGCAGCAACAGCAACCAAGCTGCAGCCATCGCCAATGCCAATGCCCCTGCAA ATGCCGAGGAAATTCAGCAACAACAACAACCAGCTCCCACTGAACCTAATGCCAACAATAATCCGCAACCGCCAACAGCA GCAGCTCCAGCACCATCACAACAACAACAACAACAGCAAGCACAGCCACAACAACCTCAACGCATACTGCCCATGAATAA CTATAGGAATGATTTGTATGATCGATCTGCCGCCGGCGGAGTCTGCTCCGCCTATGATGTTCCGAGAGCCGTGAGATCTG GCCTTGGTTACAGACGCAACTTTAGGATTGATATGCAGAATGGAAATCGATGTGGAAGCGGTCTTCGTTGTACTCCACTT TATACGAACAGTCGATCCAATTGTCAACGCCAGCGCAGTTTTGACGACACTGAGTCCACGGACGGCTACAATTTGCCTTA CGCCGGAGCGGGAACAATGCGATACGAGAACATCTATGAGCAGATTCGAGATGAGCCACTTTACCGCACATCCGCTGCGA ATCGAGTTCCGCTCTATACACGTCTCGATGTTTTGGGTCATGGAATCGGAAGAATTGAAAGGCACCTTAGCTCGTCATGC GGCAACATCGATCACTATAACTTGGGCGGACATTACGCGGTATTGGGACACTCGCACTTCGGAACCGTGGGACACATTCG ATTGAACGCCAATGGATCGGGAGTAGCAGCTCCTGGCGTAGCTGGGACCGGCACTTGCAATGTTCCAAATTGTCAAGGTT ATATGACAGCAGCTGGATCCACAGTTCCGGTGGAGTATGCAAATGTTAAGGTTCCTGTGAAAAACAGTGCCTCCAGTTTC TTCAGCTGCCTGCACGGTGAGAACTCGCAGAGTATGACCAATATTTACAAAACGTCTGGAACGGCAGCTGCCATGGCTGC CCATAATTCCCCATTGACGCCAAATGTGTCCATGGAGCGAGCATCCCGATCGGCGTCCGCGGGGGCAGCTGGATCAGCAG CAGCAGCTGTTGAAGAGCATTCTGCGGCAGATAGTATTCCAAGCTCATCCAACATTAATGCCAATCGGACAACAGGAGCC ATTCCGAAAGTGAAAACAGCTAACAAACCCGCTAAGGAGTCGGGAGGATCCTCCACGGCCGCATCGCCAATTTTGGATAA AACTACATCCACTGGATCGGGAAAGAGCGTAACCTTGGCGAAGAAAACTTCTACAGCAGCAGCACGCTCGAGCTCATCTG GAGATACCAATGGCAATGGGACTTTGAATCGTATCTCCAAGTCCAGTTTGCAATGGCTACTAGTAAACAAATGGCTGCCG CTGTGGATTGGCCAGGGACCCGATTGCAAGGTAATTGACTTCAACTTCATGTTTTCCCGTGACTGCGTGAGTTGCGATAC AGCTTCAGTGGCATCTCAGATGTCAAATCCGTATGGCACACCCCGTTTGAGTGGTCTTCCCCAGGATATGGTACGCTTCC AGAGTTCCTGCGCTGGAGCTTGTGCGGCAGCCGGAGCAGCTTCTACAATACGAAGAGACGCAAATGCATCAGCTCGCCCA CTGCACAGCACTTTGAGTCGCCTGAGGAACGGTGAAAAGAGAAACCCCAATAGGGTGGCTGGTAACTATCAGTACGAGGA TCCTTCCTACGAAAATGTGCACGTTCAGTGGCAGAATGGTTTTGAATTCGGAAGATCCCGCGACTACGATCCCAATTCCA CCTATCATCAGCAGCGCCCTCTGCTGCAACGGGCTAGATCGGAGAGTCCCACCTTCAGCAATCAGCAGAGGCGACTTCAG CGGCAAGGAGCTCAAGCTCAACAGCAATCGCAGCAGCCTAAGCCTCCTGGGTCGCCAGATCCCTACAAGAACTACAAACT TAATGCTGATAACAACACCTTCAAGCCTAAGCCTATCGCTGCCGATGAACTCGAGGGAGCTGTTGGTGGAGCTGTAGCTG AAATAGCGCTTCCTGAAGTGGATATCGAAGTTGTAGATCCTGTCAGCTTAAGTGATAACGAGACTGAGACAACCAGCAGT CAAAACAACCTTCCAAGCACTACCAACAGCAATAATCTGAACGAGCACAATGATTAAATGTGGATAAAATAAGTTGCTTC TGGTAGATCAGCGTTGTAGTTAAAATATTTAAGTATGGAGTTAAACTTCTCTCAAACTCATAAATTTTGTACGGCTTTTA CGATTTTATATTAAAAGTAAGCTGGGTTTTATATTTATATTCAAAAGTGATACCAGTTTATAGTAGAAAGACCAGTTTTT ACTTCAGTTTTCAATTCGCTATGTGCAAGGAATTTTTGTAAGATTTTATGTCTCCAGGGACAGAGAACTTTTACTGTGCG GAATTTCTAATTACAACGCTGCTCTACAAAATTGGATCTCAAAACAATACAAGCATAACGAATCGACCAAATGAACTTCG AGCAAAATTATAAATTCATTTTAAAAATCAAAAAGATATACACTTAAACATTGTAGAATTTGATATTACTCTAGAATCGG GCGACTTTGTGTGACCAGCCAACGCTCAAGATCGTATATTATTATGTTGCATACTGTAGTTGTAGTTGAAACAAGAATTT TTGTGTGTCATGTCGAGTTATAGGTAACCTAAGAGCCACATCATATCACTCTCCGTAACCACTTTAAGCAATATGCATTT GATATGATAGTTCCATTATTTTTCCACTGCCTACTCAAATTTCCATTACCCCATGGCATTTGGCACATGGACCGCTTCGT TTCAGTATGTTAAGTTATGGCTTTAAACCGGAACAATAATATTTAGCCAACTATTGTACAAGCTCTCCACTAGAATCCTA TTGTTTAAGTCAATTAGAGTTACAGGTATTATTTACATGCATATACATATATACAAGCGCATATACAATACTCCAGATCG GTACATAAATATAATCATACTTACAAATGTATTGAGTTTAACTAATTCATAATAAAATAGTTCAACGAAAAAAAAAAAAA AAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.