Clone Dmel\LD28013

General Information
Symbol	Dmel\LD28013	Species	D. melanogaster
Name	LD28013	FlyBase ID	FBcl0171099
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)

Collection Status	BDGP
Known Problems	incomplete CDS
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	6284
GenBank	AY095038
Full Length sequence data	CCTGAGAGCAGGTTACCCTACGCTCTTCGGCGCTGATTTGGAAACGGTGG TGAATCGGGAGTCTAAGAATGCTCCCGGAAGTGCACCTGTTCCCATCGTA TTAAGGCGCTGTGTGGAGGAGGTGGAACGGCGTGGTCTTGATATAATCGG GTTGTACCGCCTGTGCGGCTCGGCTACCAAGAAGCGATTGCTACGCGAGG CCTTTGAGCGCAATAGCCGTGCAGTGGAATTGACCCCGGAACATGTTCCT GACATCAACGTCATCACCGGCGTCCTCAAGGATTACCTTAGGGAGCTGCC CGAGCCGCTGTTCACGCGCTGTCTTTTCCAGATGACGGTGGATGCCTTAG CTGTCTGCCTGCCAGATGACCCAGAGGGCAATGCGAAACTGATGCTTAGC ATACTCGACTGCCTACCGCGGGCGAACAGGGCCACCCTGGTATTCCTTCT TGATCACCTCTCGCTGGTCGTTTCAAACTCGGAGCGGAACAAGATGTCAG CCCAGGCGTTGGCCACCGTGATGGGCCCACCGCTGATGCTGCATTCGGCG AGTGCGCAGCCGGGTGCTGACATCGATCACGCTCAGCCGATCGCGGTGCT TAAGTATCTTCTGCAGATCTGGCCGCAGCCGCAGGCGCAGCATCAGCAGA TGGCGCAGCACATGGGCGGCGCTGCGGGGGCAATGATGGGCGGTTTGGTC ACCGCCGGGAGCATGAGCAATATGGCCGGTGTTGCTTCAGGTCGGCGCGG CGAGTCAACAGGGCAGCGTGGAAGCAAAGTCAGTGCGTTGCCAGCGGACA GACAGCAACTTCTACTGCAGCAGCAGGCGCAGCTCATGGCGGCGGGCAAT CTTCTGCGCTCGTCCACTTCGGTAACCAACATACTCTCCCAAGGCCATCC TCAGCTCTCAGCCACAGCCAACAATCATCTGTATCAATCAGTAGTGGGTC AGTTAGCTCAATCGCATCGAGCCTTGCAACAAGCGGTGCAACAGCCCTAT CAATTGGGGGGCTCAGTGGGCTCAGCAATTCCCGACCCATCGCCACTGCC ACTTCCAGGCACACCCTCCCCCGGCAGTAGCTCAGCATCGACGGGCTCAG GCTCCGGATCGGGTAAAAGCACGGACACCATCAAGCGCGGTGCTTCGCCT GTTTCCGTTAAGCAAGTCAAGATCGTCGACCAGCCATCCAGCCCATACTC CATTGTGATGAAGAAACCGCCGTTGCAAAAAGATGCGCCCGTTGAAATCA CCACCCCCACCACACAGGCTGATACAGAGTCGACCTTGGGATGCAAGGAA AGCAATGGAACAGCTTCTCGAAGGGGAAATGTAGACTTTTATGATACGCA TAAGACGCAGGCAAAGAGTGTGGTAAACGAGGAATCCAGCTACAGCTCCA AGTACACTGGCTCGGAGACCAAGAAGATCATCCCTGGCAACAGTAGCTAC ACTCCCAGCAAGGCGAATGCTAGTGGCCTTTCTGGCGGCGAAGACTACAA AGCAATGCGTAACAAGTCGAGCGCTACATCCAGCTCCAGTTCATCGCAGG CAACCGTTTTGAGTGCTGGCTCTACCGCCACATCAGCCCCGACCACTTCT TCAGACGATTCGGATGACCTGGTCTCCTACAAGTCATCGGCGTCCACAAA TGCCTTGCTGGCCCAATCGCAGGCCATGACCACCAGCCAGCTGATGTCCA AGTATCTTAAGCGGGAGCCACGAGTACAGTTTACGCCAATCAAGTCACCG GAATCTCCCTCGCCACCGGGTAGCGGCGATGGTCTGCCGAAGGGTACTTA CCAACTAGTTACGCCTATCTCGGGATCGTCGAGCAAACCGGGTGCTACAA CTGGAGCTATAAGCAAGTATACCACCGGCTCAGTGGAATCATCTATTAAT GCGAACAGCCAGAAGTTGTCATCGCCCTCACGATTGTGCAACAGCAAGGA TAGCAATAGCAGGACTGGAACGGCCAGTAGTACGACCCCCGCGACCTCCA TGGTTTCTACAGGTCGGCGTTTGTTTGACAGTCTCGCCTCTTCATCGTCC TCGGAAACGGAGACCAAGACGTACATAGGTGGCACCACTGCAGCCAGTGG AGCCATTACCACCACCATCTACACCAACGACACCAAGAACTCCGGCAGCA GTAGCAGTAAGTCGGGAATAGGAGGAGGGTCTGGAACTGGGTTGGGAGCA GTTTCGGGAGCAAGCTCCGAGACCCGAAGCTTCGGCAGTACGCTATTTGG CAGTAGTGGCTTAGGTAATGGCAATGGTAGTAGCCACAACCACTCTAGCG CCAGTCCAAGTCCCTTCACCACCACCAACGGCAACGGTAACCACAATACC ATGCATCTGTACGGCACCTTGCCCAAAAACGGAACTTCCACGGGGGCAGC TCTGTTTGGCGGATCGGCCAACAGTTCTTCGTACCACTCTTCGGCGAGTG GAAGTGGAGCCGGAACCGCCAGCAGTAGTGGGGTCAGCTCTATGACTGGC TCCACAAACAGCTACGACTTCTACACGAGCACAAGTTCCACTGTGAGCAG TTCGCGCCCCTTTGCCAATGGAGGCAACAACTATCACACACTGGGAACCT ATAGGGCTCAGTACGCCGCCACTAACCCCTTCCTGGACGCATTTGATGAA AAGCCGGGCAGCAACGGGGGTAATGCCCACGGGGAGGAGAAGCTAGGCGC GGACAAGGGCCACCACAGAGCGGCCGTTATGGCCTTTCAGTCTTCAGGAG ACTCGAAAAACGGTAGCGATGAGTACGATGACATCAAGTGAGGTAGGAAA AATGCTATAAATACGGTTAGGGGCAAGGAATATAGATACAACTACGATTA TACGCGTACAATTGGCGAAAGTTAAAAAAATCTCAAAGCTAGTCTTCCTT ATTTAATCTTTCTTAGTCTTTCTTAGTTCACCTTCACCTTTTCGGTCTGC GAAAATGGATCGTTTAAAATATTTGTGGGCATCGAAAAGCGATCAACTGT CAACTGCTCGACTGCAGTGCCCTTGATTAAACTTTATTTCCCACTCTATC AATTTTATCGGAAAACATCAAGGCGATCTTACCCTTTCGCCATTGCATTG CTCGCGTACTATCGTAGTTATGTAACAACTAATGTCACACAATGCTGAAA GTGTTCTTTTGAAGTATTTACAGTTTACAAATTTACAAAGTACGATCGAT AATTGCATATAACGCCACAATGAGGTCCCTTTAAGCTTTAAAGCTAGCAT AATCTACATACAAAAACTAAAATAGTCGCCGAGCTCTGTGTATGTGTTTA TAAATACTTGTGAAAGCTTATCCCTGATAGCGTTAAAAGTCTTGAAGCGC TTGACAGTGGTTTGCAGTTTAGCTAACATAAATGCGCATTCAAAAATATT TACGAACAGTATTTTTAATGAACTAAAATATACATTGAATAAAGTCAACA GCGTATACAATAAATGACACTTAAGAAATACAAATAGAAGAACTTACCCA AAAGGTTGAATGCGCATTTAATTTTTCAAAACATACTTCAATATCAACTT ACACCGTGATTCGTTACTGATATTTCGCAGTGGAAACTGTATATAAGGAG AGCTATTATAATCTACCCACGTAATCTCTTTTGTGGGCGTTTAAATGTGA CCAACTCTCTTATCTGCAAAAGCAATTTACACGACTATGACAACCTGTCC AATTAAATGTTCTACGACTTTTTGAGCAACATATTTGCCTAAAATGTTTC GCCCCATGCCTCTTTTATAAAACTTAAATATGCGCATGTGATCAAGTAAG ATCGATCTCCCGATTAGGGTTTCACATAGCAGACACCACTCGCAAACTGA CATGCAAAATACAAATACATGAATTTATTATCGAATCAATGTGCTTAGTT TTTGTATGAAACAAAGTCTGATATATACACTAATATACAACGGTACAGTA TAATATTTCTAAACATTTAATCATAATTATACAAGCGATGAAAATTTAAT ATTAATATGTATTACTAAATAGCAAGTTAAGAGCGCTTTGCGATTAAGAA GTTTACAGCAGGTATGTTTTCAAGGCTTTTTAAAACGTATTAATGACCAA CTGTGCTTAACCCCAATTGTATAGGAAATTACATTTAAATGACCCTATAA CGATCTCAATTTTATAAAACATTAAATTTATAATACTTTCGAATGCATAT GTTGCGGAACTGTAAATACCCAAAAAGGAATTTACCGATCCCGTAGTCAA GTAATCAGTAATTAAAGCTGGTTATAGTCATAGGCGTTGGAAAACAGCCT ACTGCTCTGATTATTTAACTCAAGTTAGACAGAATCATTCTAAGTAACAT CCGACTAAAATAGGAAAAAACAGAAAGATTTATGTTAGCTAACAAAAATG TATAATCATTCGTCTTCATGCAAGTACAACTTTCTTCAGCGACTAAATAA AATCAATTAAAGTGAAAAGCCTCACTTATTACTTCACAGTACATTCATTG CCATAACTAATCGAAGTTATATAACCTTTAATCAATATGGCGGATTATTT GATTTTAACAATTAGTACTGCTTCTTATAAAGATCGATTGGGAACCCCAA ACACAATAAGATTTTAAATAGTAAATTATTCGGTTAAATGTTGCCAGATT TAATTTATAAGAATGGATAGCATATAAAGTGTTAAACATGATTACCAAAC CAAAGAGTTTAAAACCACGAAATAGGCAACCATATAGTTGAGCAAATTGA TTTTTATATAGTAAATTGTTAATTATTGCATATAGCACGTAGAATTTTTA ACAAAAGTAAAAAGAAATCGGAAGTTCAGGCGGGTTAGTTATTTTGTGGT GGTGTGACTTTCTTGCCGCCATTGCTGCCCAACTTCTTTATTGATTTTAC GGTTTTAACTTCATGCATAACACAGAAACATCAATGTTACTAAAGCTGTC GCCTTCGTTTTAAACACAAGTAAGACCTAGTACCCCTATTAGCTCTGCAA CCCGGTTTTTTTGTTGGTTTATCCCGAAAGCTTTAATCGTTGAACGACCA GACTTACACGGTTACAAATTCTATAAATATTAATTATATACCATATAATA GTAGCAGAGTTTGTGGAGGATATTATGACAGCACTTCGGTTTATTTGCGA ATTGTTGTGGCTTCATAGTCGTAGTTAGTTAGCATTAGATGTAAAACGTG GCGGAGATTCGAACGTTGTCAGTTGGTGGTGGAAGAAGGAAGTGGAGGCA GCGACTGCAGTTTCATCGGTTTGTGGTTTGCCGCTTGGAGCTTCTTTACT GGGCCCGATTTTGTTCGATATCTATTTGTACTTGTACTTAGAACTCGTAA GCATACACTATGGAATATATATATCACTGTGTCATATCGTACATTTAAAG CAAATAACGAAAACAGATTGCAGCTGAACGAATTAGCAGAGGAGAGAAAA ATTGTTAACGAAAAGTATTCGTGTATCGAAGTATGTATACAAACCTAAAT TAATAGCAGTCAAGCAGCAACTACATAATATAAATAGATAAATACCAAAC AAACAAACAAAAACTCGATTGGCGGGAAACCAAGTACTACAAATTGTACA TGCATTTAATCTAGAATAGCTACAACTAACTAAACACTAATTATCTGATA CTGAAACCAATCCAACTTTTAGCTGCAAACGAAAATGGCAAATGAATTGA TAAATATGCTAACACATTAGACATTAAATTAGTTAACGGTGAATTAACGG AAACGAACTAATTTATTGAAATGTATTGCACAATATCGAACACTTAAACA TATTAGAGAAAGTAACTAAGCCTACTACAGGTAATATGCATACGAATAGA ATCGGACACATAGGCAAAGTTGGCAGGATGCAGATTCAGATTGTGTTCAA AACCTAATCTCCAAGTAAAATCGATCGATTCCTCGCAGGTACTTTCGGAT AGTGAGATCGGAATCTGTTGTACCACGATTTGGCTAGTTTCGATATATGT AAAGATTAAGTGTGTTTATGTGAGCATTAAATGCCTATACTTTCGTTTAA TTTAAAACTAAAAATGCCCTTTCGCACCCGCACCTTTGTATACTTTATTT TTGCCCACCTTAATAACATTTTGACGCTTTACGGGGATTTTTGTCATGGT TTTTCGAGCGGTTTGTAAGGAATGCAATTAAGGAAAAGATTTCTTCTGTC GAACTTAATAAGTAAATGGAGACCAAATGCTCAGGGCTTTAGCAAGTTAT AGTAGGCGTAATGTACAAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000)
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]