FB2025_01 , released February 20, 2025
Clone: Dmel\LD27389
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General Information
Symbol
Dmel\LD27389
Species
D. melanogaster
Name
LD27389
FlyBase ID
FBcl0173701
Feature type
cDNA_clone
Computed gene(s)
Dmel\PheRS-m
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    LD_pOT2_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      1,505
      NCBI
      AY058580
      Full Length sequence dataSequence Downloader
      CTTCCAAGTTATAACAAAAAATGCAATAAATAGTTGCATCGAATCAATAAAGTCCCTCCAATATGCTTCTGACGCTTCGA
GTGCAGGGAGCACGGCACTGGTTGAAGTCCACCCGCTGCCTGGCCAGCAGTGCGGCGCCCGCGAAGTCGCCGTCATCGCC
ACCGCAGCTGGAGGTCAGTGGAAGCACGTACGCCACCGACGGATGGACGAACGTAACGCCCAAGATACTCTCCTACGTGG
GGGCCAACAAGCACCTGCAGACGGACCACCCGCTTTCCATCATCCGCCAGAGGATCGTAAACTACTTTTATGGAGCGTAT
CGCAACCAAAGGGGCAATCCACTATTCTCCGTCTATGATCAAATGAATCCGGTGGTTACTGTGCAGCAGAACTTTGACAA
TCTGCTAATTCCCGCCGATCATGTGAGTCGACAGAAATCCGATTGCTATTACATCAATCAGCAGCATCTGCTGCGCGCCC
ACACCACCGCCCATCAGGTGGAATTGATCTCGGGCGGGCTGGACAACTTTCTGGTCGTGGGCGAGGTGTATCGACGGGAC
GAAATCGATAGCACCCACTATCCGGTATTCCACCAGGCGGACGCTGTGCGGCTGGTCACCAAAGACAAGCTTTTCGAGCG
CAATCCTGGCCTGGAACTCTTTGAAGAGACGTGGTCGGGTACGCTGGCCGACCCCAAGCTGATTCTGCCCTCATCCAAGT
TCATGGACCAAACCAAACAGCCCTGCCACACGCTCGAGGCCGTCAAGTTGATGGAGCACGAGATGAAGCACGTGCTGGTT
GGCCTGACCAAGGATCTGTTTGGGCCGCGCATCAAGTACAGATGGGTGGACACCTATTTTCCCTTCACCCAGCCCTCCTG
GGAGCTGGAGATCTATTTCAAGGACAACTGGCTGGAGGTCTTAGGCTGCGGCATCATGCGCCACGAAATCCTGCAGAGAT
CGGGCGTTCATCAATCGATTGGCTACGCCTTTGGCGTCGGCCTGGAGCGACTGGCCATGGTGCTGTTCGATATACCTGAC
ATCCGGCTCTTCTGGTCGAACGACAGTGGCTTTCTGTCGCAGTTTAGCGAAAAGGATCTGCACAATCTGCCCAAGTACAA
GCCCATCTCCCACTATCCGCAGTGCACGAATGACTTGTCGTTCTGGCTGCCCCAGGACATTGAGGTGGATGCCGGCTTCT
CGCCGAACGACTTTTACGATCTAGTCCGCTCTGTAGCTGGTGATATGGTGGAGCAGATCAGTCTGGTGGACAAGTTTAAG
CACCCGAAAACGGGGAAATCGAGCGTGTGCTTCCGCATTGTTTATCGCCATATGGAGCGCACCTTAACCCAGGCGGAGGT
CAACGAAATCCACAAGCAGATCGCTAGTGCGAGCGTGGATAGTTTCAATGTGCAAATACGTTAGTCATAGTCTAGTTCCG
TTTCAATTCAAAACCAAAAAAGAAAAACACCAGTAAAGATAGCCAACAAAAAAAAAAAAAAAAAA
      Library Information (1)
      Library: LD_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

      (Rubin et al., 2000)
      Sample preparation

      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LD
      (Rubin et al., 2000)
      LD_pOT2
      (Rubin et al., 2000)
      LD_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)