Comment: 0-22 hr AEL
CAAGCCTGTGGGCGGACTTCCCGAGACTGCTACTGAGCAACTGGAACGTTTCATGGAGGTGTACAACGAGCTGGATGAAA ACAGACCCAAGGTGGAGACGATTCAGGCCCAGGGCCAGGAGTACATCAAGCGTCAGAACCAAATGAAGGTCTCGTCCAGC AACTTACAGCATACACTCCGCACGCTGAAGCAACGTTGGGATGCCGTGGTATCCCGTGCATCCGACAAGAAGATTAAGTT GGAAATTGCACTCAAGGAAGCCACCGAGTTCCATGACACACTGCAAGCCTTTGTTGAATGGCTGACCCAGGCCGAAAAGC TGTTGTCCAATGCTGAACCCGTCTCACGCGTTCTGGAAACCATTCAGGCCCAGATGGAGGAGCACAAGGTGCTGCAGAAG GATGTGAGCACTCATCGCGAAGCCATGCTGCTGCTGGACAAGAAGGGAACTCACCTTAAGTACTTCAGCCAGAAGCAGGA CGTGATCTTGATTAAGAACCTGCTCGTGTCGGTGCAACATCGTTGGGAACGAGTGGTTAGCAAGGCTGCGGAACGCACTC GTGCTCTGGACCATGGCTACAAAGAGGCTCGCGAGTTCAACGACGCCTGGAGTGGCATGATGCAGTATCTGCAGGAAACC GAACAAGTGCTGGATCAAATTATCGAGGAAGCCACAGCGTCCAAGGAACCACAAAAGATCAAGAAGTACATTGGCAAGCT GAAAGAGACGCATCGTCAACTGGGCGCCAAGCAATCGGTCTACGACGGAACCATGCGCACCGGAAAGAATCTTTTGGAGC GAGCTCCCAAGGGCGATCGCCCTGTGTTGGACAAAATGCTGATCGAACTGAAGGAACAATGGACTCGAGTGTGGTCCAAG AGCATCGATCGCCAGCGCAAGCTGGAGGAGGCTTTGCTTCTTTCTGGACAGTTCAGCGATGCTCTGGGTGAGCTTCTTGA CTGGTTGAAGAAGGCTAAGAGCCGCCTGAACGAAAATGGACCAGTCCACGGAGATTTGGAGACGGTTCAAGGTCTATGCG AGCACCACAAGCACATTGAGCAGGATCTGCAGAAGCGCGCTGCGCAAATGCAAGGAGTGCTCAAGACTGGACGCGACCTG GAGCGATCTGGAAACAATCCCGAAGTTGGACGTCAGCTCGATGAGATGCAGTCCATTTGGGAGGAGGTCAAGAGTGCCGT CGCCAAACGCGGCGAGCGTCTCCAGGTGGCCCTTGTTGATGCCGAGAAGCTTAATGCCCGTGTCCAGGCTCTCTTTGATT GGCTGGATCATGCCGAGCACAAGCTACGATATGCCAAGAATGCCCCTGATGACGAGAAGGTGTCCCGTGAAATGATGGAC ATCCACATGGACTTCATGAAGGACCTGCGAGTGCGCGAGCGTGAAAAGACCGAGACTTTTGAGTACGCCGAGGACATTAT CAACAAGGCCTATCCCGATGCCATTCCGATCATCAAGAACTGGCTTTCGATTATCCAGCAGCGTTGGGAGGAGGTGCGCC AATGGGCCATCAATCGTGAGTCGAAGCTGGAGCAGCATCTGCAGTCGCTCAAGGATTTGGACGACACCATTGAGGAGCTG TTGGCTTGGCTCAGCGGACTCGAGGGCACCTTGTTGAACCTGAAGCACGAACAGCTGCCGGACGAGATTCCACCCGTGGA GAAATTGATCGAGGACCACAAGGAGTTCATGGAAAACACTGCTCGCCGTCAAAACGAAGTGGACCGCGCATGCAAACCAA GACAGTTGCCACCAGGAGCGCGCAAGCCGTCGCGCAGCGGCAAGACGCCAGTTCGTGGCTCCAGTCACGATCTGCGTGAG CAGTCACCCGACGGCACCCTGAGGCGTCAAAGCTTTAAGGGATCTCGTGACCAGGGACTCAATGCCCGCAAGGGAAGTCG TATCACGCCCACACGTGACACGCCCGACAGAGATCGCCTGCCGCACTATGGCCCCCGATTCTCGCCAAGCACCTCGGGAC CGGAACTGGAATTCCGTTCGCCACGCGCGAAGCTTCTGTGGACCAAGTGGCGCGATGTCTGGATGCTCTCATGGGAGCGC CAGCGGCTGCTTAACGATCATCTTCTGTATCTGAAGGATGTGGAGCGTGCCCGCAACTTCAGCTGGGACGATTGGCGCAA GCGCTTCCTCAAGTACATGAACCACAAGAAGTCGCGCTTGACGGATCTGTTCCGGAAAATGGATAAGGATAACAATGGCA TGATTCCGCGCGATGTCTTCATCGATGGCATACTCAATACGAAATTCGATACATCTGGCTTGGAAATGAAGGCTGTAGCC GATTTGTTCGATCGCAATGGCGAAGGCCTCATCGACTGGCAAGAGTTCATTGCTGCCCTTCGTCCCGATTGGCAAGAGCG TAAGCCAGCCAATGATTCGGACAAAATACACGATGAGGTCAAACGTCTGGTCATGCTGTGTACCTGCCGACAGAAGTTCC GTGTGTTCCAAGTTGGCGAGGGCAAGTACAGATTTGGAGACTCCCAGAAACTGCGCCTCGTTCGTATCCTTCGCAGCACT GTTATGGTGCGCGTGGGTGGCGGTTGGGTTGCCCTGGATGAATTCCTGCAGAAGAACGATCCTTGTCGCGCCAAAGGACG CACTAACATAGAGCTACGCGAGCAATTCATATTGGCCGATGGCGTCTCGCAGAGCATGGCCGCATTCACGCCCAGACGTT CCACGCCAAATGCGGCCGCCACTGCCTCCTCCTCACCCCATGCCCACAATGGCGGCAGCTCCAACTTGCCCCCATATATG AGTGGACAGGGTCCCATCATCAAGGTACGCGAGCGCTCCGTCCGCTCGATTCCCATGTCACGACCGTCGCGCTCCTCACT TTCGGCCAGCACTCCCGACTCCCTGAGCGACAACGAGGGTAGCCATGGAGGACCTTCCGGCCGGTACACACCACGCAAGG TCACCTACACGAGCACGCGCACGGGTCTCACGCCAGGAGGCTCTCGTGCCGGCTCCAAGCCCAACTCGCGCCCGCTCAGC AGACAGGGATCAAAGCCGCCATCGCGACATGGCTCCACGCTGTCGCTAGATAGCACGGACGATCACACGCCCTCACGCAT TCCGCAACGCAAGCCTTCAACGGGCAGCACAGCCAGCGGTACCACTCCGCGTCCGGCGCGACTTTCGGTGACCACCACCA CGACTCCCGGCAGTCGTCTCAATGGCACCAGCACCATTACCCGCAAAACCGCCTCCGGATCGGCTAGTCCGGCGCCCACA AGGCGAAACATCAGCGGTAGCTCAACGCCCTCCGGGATGCAAACGCCGCGAAAGAGCTCAGCGGAGCCCACATTCAGCTC CACAATGAGACGCACTTCGCGGGGAACCACACCGACGGAGAAGCGTGAGCCATTCCGGCTATAGGAAGCCACCGTTATGG CTGAGGACGACGACGATAGAGAAGCAAAAGAATGCCAATATTTAAACCACATACAAAACACTCAACTCTACGTACTAACT ACGCACTGAAGTTCTAAACTAAACCCCCCAAAAACCACTCCCCAAACTAATCGAGGCTACATTAGCAATGAATGTAGCAG CAGGAACAGCACACAGTTCTACAAAAGAGACTAACTTTGAAGTGCCCTAATGGATTGGTGGAGCGAAACAATTTATAGAT GTTTATACTATATATATTTTGTAAAAGACACAGTTAAGATGAAAGTTATGCAGACATATAGCTAGCACACAAAGTTGATC AGACAAGCAACCTACTTACGAGTACTACTACTACTAATACTACCTTTTGCTACTACTAATTTGTAATGCCCACTACCTTT ATTCACTAGTTATACACGTTTCCAAAATTATGATTTGTATATACTTATAAAGCGAAAAGCAAGCGTAAATTAATGAAAAA TACACTCCAAGTGCTCTACGGCAATTAAACAAACAAAAACAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
