Clone Dmel\LD45876

General Information
Symbol	Dmel\LD45876	Species	D. melanogaster
Name	LD45876	FlyBase ID	FBcl0174652
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\RhoGAP1A

Collection Status	DGC gold BDGP
Known Problems	none
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	2166
GenBank	AY118968
Full Length sequence data	GTCAATCAGCGGTTGCCCATTGAATCGAATCGAAATTTAAGACGCATGGT GCGTAATTCTTTTATTGTAGAACTTGTCGATGGACATCGTAAGCTGCGGC ATCTCTTTCTTTTTAATGATGTCATCGCATGTGCCAAATACAAAGCATTA GGCCGCGATCGTATCGACTATGAGCTCAAATGGTTCATTCCGCTAAAAGA CGTATCCATTTACGAGGAAGCAGATCCGGCGGTCGAGCTTAAGGAATCTA GTCCAGCAAACATTTCGCAAGTAAAACGCAATTTGCGATCCGTTCGAGAT CAATTGGCCATGGAGGTGGCTAACAGTGGAAGTGGAGCCTTCCGTTCCGG TGATAAGTATCGCCGCAAGCTGGCGGATCTGGAATCACAGCTGGTGCTGG CAACGCCCAATTTAGTGTTGCGCCTGGGGAACAAGGCAAATAATAAAACC ATAACCTTCTTTCTTAGCTCGGATTTTGAGAGGACGCAGTGGATCGACTC AATCTTATCCCTTAAGCAAAAATGCAACCTGCCTGGAGCAAATACTATTA ATTCCCTGGAGGTTACCGCCTTCATTGTAGCCATGCAGAAGGGCATGAAA ACCGAAATGGGTTCATATCTTATGCGTAACACTAACGACGAGAGCCTGTT GGTTGGGGACCTGTACATGGGAGTTCATGGATTGGAAGGACTGGAGCAAG CGAACGACCTGTACATCTGCGTCGAGGTGGACTCCTATGGCCACTATTTT CGCAAGGCCACCACCAAGAAGATATGTCGCAGTCAAACGCCGCTCTGGAA CGAAAGCTTTATGCTGGAACTGGAGGGCAGCCAGAATGTACGCATTCTTC TTTATGAGGCTAAGGAGCGACCGCTTCTTAAAGCCAAGCACATACTTAAG TTGAGTTTAAGTTGGCTGACAGAAACAACGCAGCCGAAGTCAATTAAACT TACTGAGACCTTAGAGCTCGGCTGTAGCTTCCGTTTTATACCAGGCGAGC TCTTCCGCGGCAGCACAAAGCCAGGAGCCCTCTTCGGTGCCAAAATGAGT CAAGTATTAAAACGTGAAAAACGCGACATTCCGTTCATCATAGGCGCCTG CATTCGGGAGGTAGAGCGGCGTGGGATGCTGGAGGTTGGCTGCTACCGCG TCAGCGGTTCCGCTTCTGATTTGGCGAAGCTGAAGAAGGCCTTTGAGTCT GATGCCTATGAGGCGGAGCAGTTGCTACGCGAGGTGGACATCCATTCGGT CACTGGCATTCTGAAAACTTTTTTAAGGGAGCTCCCCGAGGCCCTTTTCA CGGATCAACTTTATCCACGATTTTTCGACACATTCAGCGCCTTTAGCAAC AACAACGAATCCACACGAATCAACGAGCTTCTAAAAGTTTTTGAGGAGTT GCCACAGGCCAACAAAGCCTCCATTACTTCGATATTAGATCATTTAATAA GAGTCCACGAAAAGGAGACAGATAACAAGATGTCGTTGCACAATCTGGCC ATGGTGTTTGGACCAACCTTGCTAAGGCCGGGCCAAACGCAGGTGAAGCA GAAGGATCCACTGGCAGCCAGCACAGTCGACGTTATGGCTCAGGCAGGAA TCCTATACTGCTTTCTTCAGGCACGGATCAAGAAGGATTAGGATTTCCAA TAATACGATGGAAGTTGATGGACAGTCTACTCATACGTCACGTCTCTATC TTAATGACAACCCCTTATATTATATAACAAACCGCCCAACTACCTTGAAA CTACGGAAAAGGCCATTAAATTCCGAAAACACCATAAAACACTGGTGCTG CCTACTGTATATACCAGAATCCGCAACTGCAATTATAAACCGAATATGAT GATATGACGTGGAAAAGGGAAACACCAGGATCTATGTAAGGATCCAGGGA TGTCACATATCAGCTCAATGTCGGGTCATCCATGTACATCGCACACCTGG AACTAGCAAGTGTTTAACACTTGTACATCGCTGCTTCTCCGTTACTGAAA ATTACTCCCATATATTACCCGTACTTTACTAGACATGTACATTTGGATGC AAGTTCTGCTTACCGTAACAAGTGCAAAAAAGAGCAACAGAAAAATTGCA AATATTTTCAACAATTAAAATATATATCTACAAACATAGGTATATAAAAA AAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0174652
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]