Clone Dmel\LD23140

General Information
Symbol	Dmel\LD23140	Species	D. melanogaster
Name	LD23140	FlyBase ID	FBcl0174790
Feature type	cDNA_clone	Created / Updated	2005-11-01/2006-08-19
Computed gene(s)	Dmel\knrl

Collection Status	DGC gold BDGP
Known Problems	none
Library	LD_pOT2
Strain	y; cn bw sp
Tissue Source	embryonic stage whole organism
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	2818
GenBank	AY075383
Full Length sequence data	TGCTCGCACGCTCGATCGCACGTCGCCGTCGTCGTTGCGTCGTCTTCGTC TTACGTCCCAGGCGTTACGTTGTTTTGGCCGCCGCTAAAAATCAACACAT GCAAATTAAGATCAAAAAATTACTCAGTTAAATAAAATGAATTGTAACAA TACTACGAATATGTATATGTAGTGCCAACTGAAATAGTAGAGTTCAGTCT GTAACACGTCACTGCAAAAAAACTCAAAAGCGGCAAACGAAAGCGGAAAT AAAGAAATTTACGGCAAGTGCGCGTGCGCAGCCAGGAGTGCCCTCACACA AAGGTGTGGTCACTCCATAAAAACTCGCATTCAAAACACAGAAGGCGTAA AAACAGCACCAAATAAAGTCCCACGGAAATATCTCTAGCTAAATTAAAAT TTAACCATACTGCAAAACTTTAAAACTTTGTATTACAATAAAAATTATAC AAAATATTGCCTCCTTGTGATATATGTACACCCTGCTATATGCAAAAACA CACATACTCAAACAACCCAAAAGATATATATTAAACATGCTTCACGCTCG CCTAAAGTTCTAAAAGTCAAGTGCAATTTGAGCAAAAACAGCAACAACTC TATACGCTCAAGTGACGTTTTATTGGCGCAAGGTGAACAAAAAATAATAG CAAGCAAATACACGAAAATACACAAAACCTTAAACGGAAAGTTCACAATG TTAGGAGACGCAGACCTTAAGTGCGCAACGAAATAGAAACACTAAGCAAA TAAATTGGATTAACTTGATAGGCTGATCGGTCAGTAAAAGCAGTTCTTTC GGTTGAGCAACACGGGCTCGGTGATTCACGATTACCATAATGATGAATCA GGATAATCCATATGCGATGAATCAGACGTGCAAGGTGTGTGGAGAGCCGG CGGCTGGATTCCATTTCGGAGCCTTTACATGCGAGGGCTGCAAGTCCTTC TTCGGCCGATCGTACAACAATCTGTCGTCGATATCCGACTGCAAGAACAA CGGCGAGTGCATCATCAACAAGAAGAACCGAACCGCCTGCAAGGCGTGCC GTCTGAAGAAGTGCCTCATGGTGGGCATGTCGAAGAGTGGATCCCGATAT GGTCGTCGCTCCAACTGGTTCAAGATCCACTGTCTGCTGCAGGAGCAGCA GCAGCAGGCGGTGGCTGCGATGGCGGCGCACCACAATAGCCAGCAGGCGG GTGGTGGAAGTTCCGGTGGATCGGGCGGTGGTCAAGGAATGCCCAACGGG GTCAAGGGCATGTCGGGTGTACCCCCACCGGCGGCTGCAGCAGCTGCTCT GGGAATGCTCGGGCATCCTGGTGGTTATCCGGGTCTGTATGCCGTTGCCA ATGCTGGCGGCTCGTCACGGAGTAAAGAGGAGCTAATGATGTTGGGATTG GATGGCAGCGTGGAGTACGGCTCCCATAAACATCCGGTGGTGGCATCACC CTCCGTAAGTTCCCCGGATTCCCACAACTCCGACAGCTCTGTGGAGGTGA GTTCGGTGCGAGGCAACCCACTCCTACACTTGGGCGGAAAATCCAATTCG GGAGGATCTTCAAGTGGTGCCGATGGCAGTCATTCGGGAGGAGGAGGAGG AGGAGGAGGAGGAGTGACGCCCGGCAGACCGCCACAGATGCGCAAGGATT TGTCGCCATTCCTGCCGCTGCCCTTCCCAGGCCTAGCATCAATGCCCGTG ATGCCACCACCCGCGTTCCTGCCTCCCTCGCACCTACTCTTCCCCGGCTA CCACCCCGCCCTGTACTCGCACCACCAGGGCTTGCTGAAGCCAACGCCAG AGCAACAACAGGCAGCGGTGGCTGCGGCGGCCGTGCAACATCTATTCAAC TCCAGTGGTGCAGGCCAGCGTTTCGCACCGGGCACTTCACCATTTGCCAA CCACCAGCAGCACCACAAGGAAGAGGATCAGCCAGCACCAGCCAGAAGTC CAAGTACCCATGCGAACAACAACCACCTGCTAACGAATGGTGGTGCTGCC GATGAGCTAACCAAACGCTTCTATTTAGACGCTGTGCTCAAGTCCCAGCA ACAAAGTCCTCCGCCGACCACCAAACTACCGCCACACTCCAAGCAGGATT ACTCAATTTCAGCTCTGGTCACACCAAACTCGGAAAGTGGCAGGGAGAGA GTGAAAAGTCGCCAGAACGAAGAGGACGACGAGGCGAGAGCGGATGGAAT TATTGATGGCGCCGAGCACGACGATGAGGAGGAGGATCTGGTGGTGTCCA TGACGCCACCCCACTCACCCGCTCAACAAGAAGAGCGAACCCCAGCCGGC GAGGATCCCAGACCCAGTCCCGGTCAAGATAATCCCATTGATCTGAGCAT GAAGACCACCGGCAGTTCCCTGAGCAGTAAAAGTAGTAGCCCAGAAATCG AACCAGAAACCGAGATCTCTAGCGATGTAGAAAAGAACGATACAGATGAT GACGATGAGGATCTGAAAGTAACGCCTGAAGAGGAGATATCAGTTCGAGA AACGGCTGATCCGGAGATAGAGGAAGATCACTCCAGCACCACAGAGACAG CAAAGACCAGCATTGAAAATACCCACAATAACAACAACAGTATCAGCAAC AACAATAACAATAATAATAATAATAATAATAGTATCCTAAGCGATAGCGA GGCCAGTGAAACGATCAAGAGGAAACTGGACGAACTCATAGAGGCCAGCA GTGAGAATGGAAAACGTCTGAGGTTGGAAGCCCCCGTCAAAGTGGCCACC TCGAATGCCTTGGATCTAACCACTAAGGTCTGAAAACATCTAAAAATCTG AAAAAAAAAAAAAAAAAA
Library Information
Comments	mRNA source--0-22hr embryos, from an isogenic y; cn bw sp strain, polyA+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
Library Synonyms
Reported As
Symbol Synonym	LD (Rubin et al., 2000) LD_pOT2 (Rubin et al., 2000, )
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0174790
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 1 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]