Comment: 0-22 hr AEL
TGACTCCGTTTTGAAAATTTGTTTATCCAATTTAAAGCCGTGACCGGAACCCTGCTGAGCTAATTTGTATATACAGTGTA CATAGAGATGAGCACACAAACTCGTTCAGGCGGCGGGGGAGGCGGCGGCCATGCCCGCAACCAGAAAAAGTCAAATGCCA GCAACTCCGGCGGAGGAGGAACCGGCCATCACGATGGAGTCTCACATGCTGCGGCCGCTGGCAAGAAGGGAGGCCAGGAT GCCAGCAAGACAGACAAGCCAGAGAAGGCCCAGCCCAAGGCTACCACCGAACAGTTGCGCATTGCCCAGATCACCAATAG CACCACAGAGGATCCGCAGATCAACGAGAAGGTTCTCCTTCTTTTGACCATGACCCAACGTTCCGAGGAGGAGGTCTGCT GTGCCCTCAACGAGTGCGATTACGACCTGGAGGCCGCGGCCAACTTCTTGATCGAGGAGCTACCGCAGGGCGCCTTTGCC AAGTACGAGAAAAAGCGCAAGAACAAGGCTGCAAATAACACGGCTGATGGTGCTGCTGGCGATGGCGATTGGGCCGATGG CAATGGCAATGCGGACAGGCGGGAAAAGTCGCGAAACCGCAGCTCAAATCGCGGAGGCACCCGTGGCTCCAGTGACAGTC GTGGATGGCGCGGAAGAGAGACTCGTGAGAATGAGCGCAACCAGCGCGAATCTCGCGAACCTTGGTCCGGGCAAAACGCT GGTCAGGACCGCGGTGACGATCGGGCAAATGACAACTACCGCGGGCAGCGCAACGGCGGCGGACGCAGTGGTCCCGGTGG CGGTGGACGCGGTGGTGGCTTTGTCTCTCGCTCTGGCCGTGGCGGCGGCCGCATGGGGGGACGCACCGGTGGACCGCGTG GCGATCGCGGCAGCGGAGGCCCTGGTGGTGCTTATGGATCAGGTCGCGGTGGCAATGCAAACGAGGATCACCACGAGGTT GAGCTGTGGGACAACACCATTGCCCAAAATGCCGAGAAGCAGCAACAGGCTCATGACGATGCCTGGGGCGACTGGAACAA CGAGGAGTACGAGGGCTCGCTCAAGGACAGCAAGGTGTTCACCACTAGCAACCTGGCAACGCAATCCGCTGCCAACGTGG TTAGCGGAACTGGCGCGAGTGTTACAGCTGTTCCTGCGGCGGCTGGAACCGAGATATCGGCGCCACCGGGCCTTGAACAT CAGTTGGTGCAGCAGGGATCTCATCTGGAGGAGAGCTCCAGCAGCGGCCCAGCGGCGGTCACACCGCCAGCAACGCTGAG CGGCTCGGCGACCACGCCGCTGCTGCAATACAGCGCAGCGGTCAGTAATCCACCGCCCCAGCTGCAGTCCCAGGGCACAC AGTCAGGAGCGGGTACAGGAGCGAGCGCAGCTGCCGGCGGAGGAGCAGGCAGCACACCGTCATCCTTTGTATCCGCCTCT CCCGACACATTCTCAAGCGCCGCCTCGGCAGCTGCCACGCTGGTGCATCAAGCACAAAAGCAGCAGCAACTTCAGCAGCA GACGACGCCTATCAAGCCGTCGGCTACTTTGTCGGTCGAGCAATCTCAGTATTTCAACTCGTTGGCCAGCCAGGGCGTCA GCCCAGGTTCTGTTCCAGTGCAATCGGCGCCAGCGGGTTACGCGCAAAACCCCGTGGCCGCCTACTCCCAAACTAGCACT AGCGTGGGTGTGAGCCAGTACCCCAATACCTATGCCAACGTATTCGCTTCTGGAACGGCAGCTGGAGCTGGCACTGCCGA GCAATCGCAGCAGCAACCGCAGATACGGAGGGCACGTGTTAAGCTGCCACCACCCTCGAAGATTCCTGCGAGTGCCGTCG AAATGCCCGGAGACAATGCACTGAACAACATTGGCTACCTGGACGTGCAGTTCGGCGCTCTGGACTTCGGCACGGACGAT GGCTTCGAGCCATTGCCGGAGAAGGTTGGTTCGGGCTTTAGCATTGATGGTCAGCAGCAGCAACAGCAGCCAGATGACTA CCAGAGCAAGTCCCAGCAACAGCAGCAGGTGACGCTAGCGGCAGGTCTACAGAGTTCGCAGATTAGCGATGCCTTAAATG CAGCGGGCTATACAAGCCGTTCGACGTCACAGCAGCAGCAGGGCGTTAGCTCAGCGGTGAATGCCACAATCGATCAGCTG ACCAAGAGCGATCCCTACGGACAGACGGGCGGCAGTGGTAACGCCTACCAAAACGCGTACCAGAGCAGTGGAGCGAGTAA GACAGCCAGTGGCTTTCCGACGACCGCGCCCGGTGGCTACAGCAGCTCCACATACGCGAATGTGCAAAGCTCAGTAGCCA ACAGTTACCAGCAGCAGGGATACGGCTCATACCAGCCCAGTTCCTACCAGCAGCAGGCTGGCAGCGGGGCTCAAAGCGGT ACAGGTGCGGTAAGCGGAGGCGGAGGAACGGCGACGCAAAACATTCCGGTTGGAGGCAGCAGCAGCCAAAATAGCACGAG CGGCAATGCGAGCTCTGCCTACCTCACATCCGGATATTCGACACCACAAAGTGCTTACCAGTCAAGCCAGAGTGTTTATG GCAACACTGGACTGTCCAACAGCAGCGGGTTCTCTGGCAGTGCGAGCAACGCGTCCTCGCAGTACGCCAATTTCAGTGCC AGCGCCAAGCTAAAGGATGCGACCACGGCCAGTAGCGCCGCTCATTACGACAGTGTCTCAACCAGCAGCGGAGTGAGCAG CAACAGCGGAAGCACAGGCAATGGCGGTGTGGTGAGCGGTCAAACAGGTGCTAACCAGGCGGCTGTATCGAACAATAACA GCGTGAGCGGCAGCAGCTCGGTCAGCAATGTGACGGCAGGCGTTGCGAGTGGCAACGTAGCTGGCGTGGGCGGAGGCGTA AGCCAGAGCGGCGTAAGTAGTGGAGTCGGCGTGCCCGGTGGCAGCGCGTCCAGCGTCGGTGTGAATGTGAACAACAACAG CAGCAGCGCCAGCTCGGTGGGAGCAGCGACCGTTGCCCAGACAGCCACGGGAACCACCGCTGCAGTGCTGGCCTCGCTGA CCAACAAGAACACCAGCAGCAGCAATAGCAGCGGCAGCGGTGGCAGTGCTGCCACGACGACGGGCAACGCCAGCGGACAA GGTGCGGGAGCAAGCACCGGCGGCGTGGGCAGCTCGTCGGGTGCTGGTGGTGCTGGTAGTGGTGGTGGCAGCGGTAGCGG CTTGGTGCCCACCAACATCCAAATGGTTAGTCAATATATTCAGACTGGATTGCCATACTATCAGCAACCAGTGTATTCCT ACGAGGAATTGCAAATGATGCAACAGAGAGTGCCACATGTGCAGGGATATTACGATCTGAACTATCCGCCGGCCAGTTTA GGAGCTGGACGTGACAACCTCGGCTCTGTGACGTATTCTGCTATGAATGATGGACGCTTTGCCCGCACTGACAATAACTC CAGTCCGGTCGGCAATGTCTCCAGCACAATGTCGCAACAGGCTGGCTCAAGTGCGCCCATGCTAAATGTTCCTTACGCCT ACTTCTATGGTGGCAATGTAATGCCCGGTAGTTTTCAATATGGCACGCCCGCCATATATCCACAAATACCGGCAGCAAAC ACTGCCTCCGGTCAACAGTTCCCGAAGCCTTCGTATAGCGCGGGCTACGGCTCGACCAGCTATGACACTCTGTCGCAGAC CACGCAGGACTACAGCAAGGGCGGCTACTCGTCGAGCGTGAATCAGCAGAGCAAAACTCAGACTGTGTCCAACCAGTCGC AGGCAGGCACTGGATCCGATCTGACCTCTTCTATGTACGGAAAAGGACACGTGGCGCTGAACAAGGTTAATTCGTACGAG AAGCAGAGTTTCCATTCGGGCACTCCGCCCCCGTTTAACATGCCCAACACTCAGACGGCTGGAGGCACCTCTGCCCAACC GTACGGCATGTACTTGCCGATGCCAGCGGCCGGACACCACAATATGATCCATCAGCCCATCCACCAGGACTCGAACAGTG CCGGGCAGCGTCAACAGTCGACCAGCCAGTCGAAGTCTGCTGGCAAGCAAGGCTACTCGCCCTCGTACTGGGCCGGACAG AACTAGCTTCCGGAGAACACGATCTGGCGGCCCCTGCAGATCGCCCGCGCGAACGACTTTTATAGCCTTCGCAGCAATAG CAGCAGCACCACAACAATAACAACAACAACCACCACCACCACGCCCATTCACCACATCTACAGCAAGAGCAGCGCCAGCA ATGCCCGGACGCTGCCCATCACACTCGGAACCAACTCAAAAGTGACCACAATAGCAGCCAGCAACTCCACAACGAGCGTG GATGCCAGCAACACACCAACAGCAGCAATAGTTAAGACGACAACATCGGCGATGCCGGGCATTCCCGGCCAGTTGCTGAT GCTCGTCAAGGAGCAACAGCAGCCACAACCACAGCCGCCACCACAACAACAACAACATCAAGCACTCGCCGACAAGGTGG GGAGCGAGAAATCCTCGCCAGCGTCGGAGAATGATTATCTAATATACGATGATAAGCGATCATACTTTACTTAATAAGAA ACGTTTTTACTACGAACGCACTGTAAAGGCGCCGCTCAGCAGCGTCCTGACCACCACGCCTCCCTCCTCCCCTCAGCCTC AAGACACACCACACCCCACACTTGCGGAGGCGACTCCTAAAGCCAACCACACACACAACACACTTCCTCACACAACAAAG AGTAGAACGAAACATACAGCAACATCCGCATAAATATACCAAGTACCATTTTCACATTGCATACAAATAAATTTACAAAG ATAAAGAGTAACAATTATTAATTATTAAAGGCGTAAAACGATTTAACAGATTTAAGAGTGTAAGAGAGAAACCGGAAATG CAGAACAGTAAATTAAAAATACAAGTATATATATTAAATAAACCACAAAACCACAAACATAACGTGAATGAGAAAAAACA AAACAAACAAAAATTAATTATTGTAATCGGTTTAAACTTATATGTATAATTATAACCTTAATATTTAAACTAAATACAAA AAAGAAGATACGATTAAATCCAAACAAAATGCAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.
