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General Information
Symbol
Dmel\LD39963
Species
D. melanogaster
Name
LD39963
FlyBase ID
FBcl0178498
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    LD_pOT2_cDNA
    • LD cDNA library of D. melanogaster, iso-1 strain, embryo (0-22 hr), pOT2 vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      4,394
      NCBI
      Full Length sequence dataSequence Downloader
      TTTACGCCGGTGCCGCCAATAATTTTCCGTTTTCGTTGAGGTTTTTATATTAATTTGTTGTGCTAGCAAGATGCCCACAT
      CCAAACTGCAAGCCAACATGAGCTACCGCGAGGTCATACCGCGGAACCTCAGTCCGGCGGAGTGGATCGAGGTGAAAATT
      AACACGGGAACCCAGAGCACTTTGCAGGATTTAAAAACCTTCGAAACCTCCGGCGGCTATTGCTACAAGAATACGAAAAA
      TGTTCAGACCAGAAACCGTTTCATATATTGGCGGACATACCAGGATGTCCTGGAACTATCAGAGGTTAGTTTGGACATCT
      CCCTGCAGAGGAATCACCTGCGTCTGCGATTCACCGACTCCGCTGTGCTGAATGTATCACTCACGGAGCAAGGTACATCC
      GTCACCCTGCTGGTGGTCACGGTCAGCAGTGTGCATCGCTATGTGTTTCCCTTGAAGGTCGCTGGGCAGGAAGGTGGCGC
      AGCTTCTCCAGAGGATCTGCTATCCCAGTCCATTTTCTACGACGTCAATGACAAGATCAATGATCCCAGCACTTACTATG
      TCACGGATGGCTTTGGAACTATGCCCAACGCGGCTGTATCGTACCTCACTCAAGACTCCCAGTCAGCCTACTTTGCGGTG
      GCGTATCAGAGCAAACTACTGCTTCACGTTATGAAATGCTCCACCGGCCATACTATCACCCACGAAATCAAGGAGCCGAA
      GCTAATGCCATGGTTTCTGTCGAATCTCAAGGGTGCCCTCACCGGCCGCTCTGAAACGTTGGAGGCAGCCACGTCCATGG
      CTTTCAGTGAAATCGGGGGGGAAATTTTCATTCTGGTTCTGTATCGCAATAATGAGCTGCGTCTATGGTCAGTGGATAAC
      TTGCAGACTGTAGCCAGCATTAACTGCTCCACCGAACTGGGACAGGACTCAGCAGCTCAAGGACCACAAAACAGCCAGCT
      CCGAAAAATAAGCGATCAAAACTTCTGTCTATTTCTCTCGCACAATTCCCGAGCTGAGTTTATATGCGTTAGCATAATGC
      CCGATGCCGACGATGCCAGCGTTATCAATTTGGTGCAGAACATGGTGCCGGCGCCGCAAACGGATTTGGTCGACTTCGAT
      GCCACATCCTCGCACATCTGGGCCCTTTGGAGCAACGCTGAAGGCGATTTCCATGTCTCGGCAGCATATTTTGCCTCCAA
      CAACGCAATCAAATGGGTTTCGGCTGCTCTAGAACCGCCACCGGATCGGTATTGTCTCACCATGGAGCAGGGAGTGGATC
      CACGCGAAACTTACTGCAGCTATATATTTCATCCTGGTCGATTTGACCGAAATGTAATTGCCAAGGCTTTATATATGTTC
      AGACGCGTTAACCTGCAATTCGATGTAAAGCAACTTTCCATGTCAGTGCTCAAGGAGCAGGTATGCCAGGCCGTGGAAGA
      TGAAATCCAAAACGAACTGAAAGACTTTGTGGTCACCGATGAGGAGTACTTGGAGATATCCACTCGGCTGTGGGATCGCT
      TTTACTCCTGCTGCGAACAGTATCACATTAAGCTTTCCGAGCCGACAGGACTCGCCGTTCTTGGTGGAATGGATGCAGTA
      TGTTTGGTTAGAAGGCAATCATTCGCATTGCTGCGCCCCTGCGAAGTGCTCGAGCACCTGTTGCTCATCGGCGAGCATAA
      CGATGAAGTGGCGACATATGTGGCGCCACTATTTCGCAACGATCCCGAAATGGCCAAGGGTTTTGTGGAGCTCATGAATG
      TGGTGACACTGCTTGATAAACTAATTTCGGAGGACATTAAAATCGAGCTAGATAAACAGCTTTACCAGCGGGAGTCACCG
      GTTGAAGTAATATCTAAGCTGGTGGCTAGAATCAGTATGATCGATGATAATGGTCCAATATTGCCATCAAACTGTGTGAG
      GCAAATCCAACAGAAGCTGCAGAATATACCAAATCTTGAACCCGCTTTGGAAATGCTGCTCGATGTGCTGTGTATGATTG
      ATCCAGATGAACCGCCGCATGATTACTCCCTTTCTACGCGCTTCTTGCAGTCGTCTGGGGCTCTAATGGGTAGCGAATAT
      GGACTTTCTATTTTGTCCGAAACAGTGAAGCAAATGGCAATGATACGGTTTTCTGTTTGCCGAAACCTTTTGGTATTGCA
      GTACATGGCTTATGGTCAGAACGAAATGGAAAGCGAAAATGTTTTAACAAATTTAAATTATTTGAACTCATATTATACAC
      TGGTTTGGATTGCCGAGACACCCATTTCATCGAGTACCCCAGCTGGATTCGAGGCATCTATTCAACGATTGAGCCGCGCC
      CAACTTTTCAGCGGCTATAATCGACCATACTCCAGCCATCTGAAACATAATGGGAACGATCAGACCACTCTGCTCCGACT
      GTTCCTGGAATCGAAAGGTCTGTTCAGCGCCTTGACTATGCTGCTGAAGCATGACAGTTTGTCGCTGGACTCGGAGCAAT
      TGAATCTACGCCAGTCTTTGCTCCAACTTGTTGGATACATCAATAAGATGCTTTGGCCGGGATCTCCGATTTACGTATTT
      CCGGAATGGTTATTTGGGACCTGTCATCACATCATTGTTCAGGATTACGTGCGCATTTTGTCAAACTGGTGTTCCGTACA
      AAAACATGCCAGACGCTTTATGCTAGCCGTATCTCTATTGGATTGCGGTGAGGCCCACAAGGCCGTGCATCTCTTTCACG
      AGGCGGAGTCTGGCATTGTGGAGGATGACTTCCTCTTTGAGCATGTACTGAAAAATACCCCTCTATACGGCAAATTACAG
      AATAGCGTCAGCCGTGGCGATACAATTTCCCCCGAAGACACAAAACTGGCAATTGTTCACTACTATCTTAAGGTTATCCA
      GCTCTTTGAACAGCATTCCGCATTGGACTACATTATTCAACTGGCTGACATGGCCATTAGGGTGCTGCAGCCAGATGATC
      CTCAGTTGCCCATGTTCCAGTCAATAGTGTTCAATAATCATCTGCAGTTGGGACACTACGTGGAGGCGTACACCGCCTTA
      GTCAACAATGCAGATATTTCAAGGCGTAAGGATTGCCTTCGCCAGTTGGTCATAACGCTGTTCCAGAATAAATGTCTGCC
      ACTGCTTATGCAGTTCTCCTACATCGGGTTGCAAGACGAGTTCGAGAGCATTGTTGAATCCCGGGCACGATCAATGAGCA
      TCGATCAGAACGAAGTCTACAACTTTTTGTATGCATTCCATACGAACAAGGGTAACATGAGAAAAGCTTCTACGGTTATG
      TATGAGCAGGCCATGCGGTTTCAAGTAGACAGCGATGCACCAAACGCGCTAGAGAAACGTTGCTCCTCGCTGTTAATTTG
      CCTAAACTGTTTGCACCTAGTTGACAGCCGCTACAGATGGATTGCCAAGCCTGTTCTTGGTGATGAGCAGGTGATTACCA
      TAGATCAGGATAACGACGATGGTGAGCCCAAATGTGACGAGGACAAGAGAGGCCAGGAGGTGGTTGTTCTAGAGCTGGCT
      GATATCCGAAGGGAACTGGTGCACGCAGAAGCTTTACGTGAGCTTTCCTTCTACCGCAAAGATACAGCTGCCTACGAGCG
      GGCCACACCCGAGGAGCTATCATACCTGCTGGCTAGTTCCGGCCTGTACACCGCGGCATTGAAACTTTCGCGGGGACACT
      CGTTCTCTGTGCTCCCCATATTCGAGAGTCTGACATCCGCTTGTGTTGCTGCCACTGAGGACAAGTCGTCGGACGCCTGG
      AACTGGCTGCAAAACAACGACATGGCAGATCTACCTCATCGAAGTAATGCTGCCGATATGGCCTGGACTTTACTTCAAAA
      ACTGGTTGTGGATAACGAGGCAAAAGACTCCACCTTGATAAGAAAGAGCGTGGTTCAAAGGCTTCTCGGGTTAAACGCCT
      TCTTGCCCCAGTGGCTGATAAACTCCTACAAGTTATCCCACTCCCGGGAACTGCTCCACTTACTTGTGAAACACAATCGT
      CTGCTGGAAGCCGCAGATCTGGGATGTGAAATAATCGCCGGCATGCTGGGAGCAGGTAGCGAGTATTTCGAATTCAAACA
      CGCGGTGAACATAGCAAACCCACAGCTCTGCTTCCCCATCAGCACGATCGATTTGCTGCTGCACGGTTTAAAGATTAATG
      GCAAGGATGATCTTGATTATGAAATGGCTTACTTTAAACTCGAAGAAGAAGTTCAAAGATATATTGAGACCATAAAGCGC
      ACCACCGATGATAAGATGAGCATGGCCGTTCTGCAGATGCGAACGGATCTTCAGGAAGAACGTTAATAGTTCGTTTAAAT
      CAGTTTATTTAATAAGTTTTTTGAATTAAATGCTAAAGCGTGTAAATCGTTTAAAAAAAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Library: LD_pOT2_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.

      Sample preparation

      Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD21101 and higher, cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid.

      Comment
      Reported As
      Symbol Synonyms
      LD_pOT2_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)