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General Information
Symbol
Dmel\LP04690
Species
D. melanogaster
Name
LP04690
FlyBase ID
FBcl0181495
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    LP_cDNA
    • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      2,087
      NCBI
      Full Length sequence dataSequence Downloader
      AGGGCGTGAAAATAAAATAAAATTGGTGACCGATTTAAATAAATAATAAATTCCCAAAATGAGCCTAAAATTGATGGCGT
      TTGTCTGTGCTCTGCTGCTCCTGTGCACCTTGACCCATGTCCTGTCGGCCGCGACCACAGTGCGTCCCTACAAGTTCGGC
      TTCACCATCGACGAGCAGCAGCATCGGGCAGAGAAGAGAGATGAACGCGGCATTATCATGGGCGAGTTTGGGTTCATCAC
      CGCCGATGGTATCTATCACGTGACGGTCTATGCCACCGACGAGGAGGGCAAATTCCGGATAATCTCCATGAAGAGCTATC
      CTTACGCGGGTCCAGTTGGTTCAAAAAGTGTTCCTGTGACAACGACTCCAAAGGTCCTGCTTCCTGCAGCTCCGGTGGCT
      CTGCCCAAGTACAACTTCAACTCGGAGGCCTGTTCCGGTTGTTTCCTAAAAAAGTCCCCTCCCAAGACGGAAATTCGCAC
      ACTTTCACAGCCATTGGCTCCAGTTCAACCAGGTAAGCCCGATGGCTCTTCAGACATCGGCCTGAATGTGCAGTTACCCT
      TCCGGGAATCCATTGCACAGACTGTGGCAAGTCGTCTTGGTTTGCTCCAAGATACACTGCAAACCTCCAACACAAACACG
      AACGCACCAAACACTAAAACAATCGAACTTGGTCTCAACATAGCAATGAGCACATACTACACAACAAAGAATGCTGTAAC
      CGGACATGTGAGCACGCAGACCTCCAATTCGCAAACTCCGAGTGCCAACACAAAGATCGACTACAATGTGGGCGTGGTCG
      AGAAGGCTTCACCGCCGGTTTATCGGCCATTGAACATCCGGTTGAACGAGGACGTTATGAGACAAGCTATTACATATGGA
      AACACAATACCTGGCCATGTGCCCTTGCCCAATCAGCCACTTGTTGAAACCTCATTGCTTCCAGCGAGTCAACACAACAT
      CCGAATGCGGGCCTTAATGCAAAGGTTCCACTTGCCAGTAACATTCAATCCGTAGCACAACATCCGAATGCGGGCCTTAA
      TGCAAAGGTTCCACTTGCCAGTAACATTCAATCCGTAGCACAACATCCGAATGCGGGCCTTAAAAACATTAATAGATCGG
      GAGTTTCTTCTGCGAAAACATTGGCCAATACGAAGACAAGACCACCACACACATTTAACCCACACCAAACACCTCTTTTG
      AGTTCAGCCACCGCCCCGGGAATTTCTGGAGTGACAGCGAATACACCAACCGGAAATGTACCCTCAAATGGTGGAGGCAT
      CGCGGCGGGTAAAGCGCCTGGTAATCCACAAGCTGGCGGATCTGGAGGTATTATCGGTGCAGGAGCTCCAGGCGGAAGAA
      AAGTCTCGGCTGGTGGAATTGGTTCTGGAAGTGCAATTGGAGGAGTCTCTGGAGGAAGCAAAGCCTCAGGAAACGGCGGT
      GCAATTGGATCTGGAAGTGCAATTGGAGGAGGGGCTACTGGAAGCAAAGCTTCAGGATTTGGATTCGGAAGTAATATTGG
      AGGAGGTGTTTCAGGCAGCAAACCTTCAGGGTTTGGATCCGAAAGTAAAATTGGAGGACCTGATTCAGGCAGCAAAGCTT
      TAGGATTTGGATCCGGAAGTAAAATTGGAGGAGGTATTACTGGCACCAAAGCTTCAGGATTTGGCGGAGAAATCGGTTCT
      GGCCGTGGAAGTGCCTCAAGTGCAACGGGTGATCTGTACAAATTCAAGTACATACTGGACTACAATGGACACGAGGAGAC
      CGGCGGTCGCAATGGTGACAAGCAGGGTAGCTACTTTGCAATCGGCGAGGATGCAGTGCAGCGGACCATTGAGTATATTG
      CGAACGAGTTTGGATTCCAGCCCCACGTCAGCTGGCGGAAATTGGACGCCAAGGAGGCGCTGCCCGAGGAGAATTCACTG
      AAGCACTACGAGTTTAAATGGTTTAATCAGGAATAGTAATGATACGGTGATTTTCCGGTGGATTATTCCAAGACTAGAGT
      AGGCACCTCTTTTCCCCATGCTGTACATAACAATTTTTGGTTAATTTCGAATAAATACAACGTTAAAAGAAAAAAAAAAA
      AAAAAAA
      
      Library Information (1)
      Library: LP_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

      Sample preparation

      Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

      Comment
      Reported As
      Symbol Synonyms
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)