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General Information
Symbol
Dmel\LP18923
Species
D. melanogaster
Name
LP18923
FlyBase ID
FBcl0192028
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • chimeric clone
Library
LP_cDNA
  • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    1,766
    NCBI
    Full Length sequence dataSequence Downloader
    AGTTCTTTAGTCTGACAATCTAACCAAGTCAAAATGTTCAAGATCCTGCTTGTCTGCGCCCTTGTCGCCCTGGTGGCCGC
    CAACGAGAATCCCGAGGTCAAGGAGCTGGTCAACGATGTCCAAGCCGATGGCTTCGTAAGCAAGTTGGTCCTGGACAACG
    GTTCCGCTGCTTCTGCTACCGGAGATGTCCACGGAAACATCGACGGAGTTTTCGAGTGGGTCTCCCCCGAGGGCGAACAC
    GTCCGTGTGAGCTACAAGGCCGACGAGAACGGATACCAGCCCCAGAGCGACCTCCTGCCCACTCCTCCTCCAATCCCAGA
    GGCCATCCTGAAGGCCATCGCCTACATCCAGGCCCATCCCAGCAAGGAATAAGCAATCGACACGACCAGGACCCACATTC
    GAATCGGAGGTGCAACTCCAAAGACCTTGCCCTCTAACCCTTAGAATTTAAACAGCATGCAGACATTATAAATGATTATC
    GAGTTAGGAAATAAATTCGATACTCTTTGGCAACAAAAAAAAAAAAAAAAAAACTCGAATCACACTTTAAAGACAAGGGA
    TATACCCCTAAAATATCACATAGAACACTATGCATTGGTATGCAATCCTCCAAGGATTCCTCCTGGGAATATCATCGATA
    ATCATTTTATGGGTACAAGGAGTCGTTGGCTTGCCATTGGCTACAACCGATCTGGAGTCACTTCCAATCGTCTTGGCAGA
    GGATGTTCCGGTGGAGAGAGCTCCAAGGCAAGTAAAACCAGATCCGTCGACGTTCGATATTTTCAAGGTGGATCAAAGCT
    CCAATAACCAAATGCAGCAGAAATCCGACGGCTTCAGAGTGTTCGCAAAGAAACTACAGGATGATTATCACCGTAGAGCT
    GGCTACAATGAAACTAATCCGCCACCAATTACTGTGACCCGAGGAAGCACTACTGCTCCTAAGAAAATCCATAAAAGTGG
    CATTCGACTATTTGCTTCTCCACAAGATCCACCTCCGCCTGAAGCTGCGGCGCCAGCTGATGGAGCTCCAGCGGCGGCAG
    GAGCGGCTGAAGGAGGTGCCGCCGGAGGAAAAGGAACTGAACCACCCAAAGAGCTGACCGTCAGTATTCAACAATTCGTT
    ACAAACCAATATGGGGAGAAGGAATACAATGCTTGGGGCGTCACTTTTGGTAGACATTTCTTCGACACCCGTTTCGCCGT
    AGCTAGGCATGAAATACCTCGAATAACTGTGGATCCGAAACCGGTCCGTAATTACTATCCAAAAATGGACAATCTGACCA
    CCGTCTGCTTGAACAGATCGACCATCTACAATGAGATTAAAGTCGAAAAGTGGCTGATGCGCCACGTCTACAGCATAAAG
    AAGAAAGTGCGCCCCTTCTTTTTCTCCCTTCGCCAGCAGCTCTACGAGAAAGGGGATTCGGAGCAGTGCCACAATGAGTC
    CTACACTGACTGGTTGGAGTACCAGGAGTGCGCCATTAGACGCAACCAGCGCATGGAGTACTTTATCCCCAAGTATCCGC
    TACACCAACTTGCCATTAACTAAAGGCAACCACATCTTGGACTCACTCAACCTGTTTAAATTTCAGTTAGACATTGATCA
    TGATCAGTCTTCGTACGAGAAAATTGATGGGACAAGGAATGATTATCATATTTGTGATTCCTCTTCATTTAAATGTAAAT
    TCAAAAATTTTCTTGATTTATGCTTGCAGAAATAAATATATTTTATTTTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    AAAAAA
    
    Library Information (1)
    Library: LP_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.

    Sample preparation

    Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

    Comment
    Reported As
    Symbol Synonyms
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 1 )
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)