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General Information
Symbol
Dmel\RH52407
Species
D. melanogaster
Name
RH52407
FlyBase ID
FBcl0243308
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI02090
Library
RH_cDNA
  • RH cap-trapped cDNA library of D. melanogaster, iso-1 strain, adult head.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    1,847
    NCBI
    Full Length sequence dataSequence Downloader
    GAGTGAGTCCCCAAGCGTTGTCGCGTCCACTTTATCGCTGTTTCTGTCGCGGATTCGAACCACTGACTAACGGTAGTGCT
    GCACGCATCTGGCGGTAAATTGGCAGCGAGAAGCCCGCGCTAATAATACATTCTGAAAGCCGTCGAAAGGATTGCGAGTC
    CATATCAAGCATACGCCCGATCAGCCGCCAATCGATTATCTTTTGCGATAGTAGAGCAGTGAAACAGCCAGAGAGAACGA
    CAAGATGCATGTGCCATTATAAGGATAAAGGGAAGGATAGCAACTAAGCAGGTCCTCGTTATATATGGAAACCCAAAACT
    GATATTATTATACTAGACTACAGTGAACTTGAGAGTCAACTCTTTACCCACATACTTAGAACCCACTCGCCCCCGCCCCC
    AGAAGTAACCTGCAATGGGTAAGGACTACTACAAAATCCTGGGTCTGCCCAAAACTGCCACCGACGATGAGATCAAAAAG
    GCCTACAGGAAACTGGCCCTGCGTTATCATCCGGACAAAAACAAAGCCGCCAACGCCGAGGACAAGTTCAAGGAGGTCGC
    CGAGGCGTACGAGGTTCTTTCGGACAAGAGCAAGCGGGAGGTGTACGACAAATACGGCGAGGATGGTCTCAAGAGCGGGG
    CACACGAAACGGCGGTCCGTCGAGCAACTCGTTTACATATCAGTTCCACGGCGATCCGCGGGCCACCTTCGCCCAGTTCT
    TTGGCAACAGCAATCCGTTCGCCTCGTTCTTCGACATGGGCGACAACCTGTTCGACAAGAAGGTCTTCGATCTGGACACG
    GAACCCGATTTCTTCTCCTCGCCCTTTGGCGGCATTGGGTCGCGACATGGTCTGGGAAGTGGCTTCAGGCCCTCTTTCAG
    ATCACACTCCTTCAATGTGCACACGCCGTTCAAGAAGGAGCAGAAGCAGGATCCGCCCGTCGAACACGATCTCTATGTGA
    CGCTGGAGGAGATCTACCACGGCTGTGTTAAGAAAATGAAAATCTCCCGGCGCATCGTTCAGGCGGACGGCAGTTCCCGC
    AAGGAGGAGAAGTTCCTGGCGATATCCATCAAGCCCGGCTGGAAGTCCGGCACCAAGGTCACCTTCCAGAAGGAGGGCGA
    CCAGGCGCCCGGAAAAATTCCCGCCGACATTGTGTTCATCATCCGGGACAAGCCGCACGCCATGTTCAAGCGCGAGGGCA
    GCGATCTGCGCTACACGGCGAGACTGACGCTCAAGCAGGCTTTGTGCGGCGTTGTCTTCCAAGTTCCTACCATGTCCGGC
    GACAAGTTGCGCATCAGCACCATGCAGGAGATCATCAAGCCGAACACGGTGAAGCGCATCCAGGGATACGGGCTGCCATT
    CCCCAAGGATACGACGCGCAAGGGCGACCTCCTGGTGGCCTTCGACATTCAGTTCCCGGAGAAGCTGACCGCCGCCCAGA
    AGGAGGTGCTCAAGGACATGTTATGAGGAGGGATCACTGGCCTGATCACTGACCGACGACTCCGATGCACCCGCAGTCCT
    TCGGTGAATCCAGGGACTTAGTCATCCATTCAAACGCACTAAAACCAAACCGATCCCATTCATTTTGTAACAACCCATCG
    AGATCTTGCTCCCATTTTGCTTGTTTTTGTGTTCAGTGAAACATTTGCGCATTTTTTACAGGAACTCCCTCTTAACAATC
    GGTTGTGCTCATCTGCGCCGCGAACATGTTGCATAATATGTATGTTGACCATAGTTATTATATCATTAAATCTTAATTTA
    TTTGTCTAAATTAAAGCTAAATTTAATAAAATGACACACACACGCGTACACGTGAAAGAAAAAACAAGCTAAAAAAAAAA
    AAAAAAA
    
    Library Information (1)
    Library: RH_cDNA
    Description
    Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
    Sample preparation
    For the RH cDNA library, adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
    Protocol
    RNA was polyA+ selected twice (RNA made by L. Hong). cDNA was synthesized by priming with the oligo(dT) primer adapter (5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3'). The first strand was synthesized in presence of trehalose, which increases the full-length cDNA synthesis (Carninci, P. et al. 1998. Proc. Natl. Acad. Sci. U.S.A. 95(2): 520-524; Carninci, P. et al. 1999. Methods Enzymol. 303: 19-44). Full-length cDNA was selected with the biotinylated cap-trapper (Carninci, P. et al., Genomics 37: 327-336). A linker was then ligated to the single-strand cDNA following the published protocol (Shibata, Y., et al., Biotechniques, in press). Subsequently, the cDNA was normalized by using RoT=1.0 as published (Carninci, P., et al., Genome Res. 10: 1617-1630). Second strand cDNA was primed with the (5'-AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3') primer. After restriction digestion of the hemimethylated cDNA with BamHI and XhoI, thecDNA was cloned in the lambda FLC-I vector. Subsequently, the library was bulk-excised into pFLC-I plasmid as described (Carninci, P., et al., Genomics, 2001, 77:79-90 ). cDNAs were transformed into DH5-alpha TonA strain.
    Comment
    Reported As
    Symbol Synonyms
    RH
    RH_cDNA
    Riken head library
    References (1)
    Research paper (1)
    Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
    The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)