Clone Dmel\SD04522

General Information
Symbol	Dmel\SD04522	Species	D. melanogaster
Name	SD04522	FlyBase ID	FBcl0277745
Feature type	cDNA_clone	Created / Updated	2005-11-02/2006-08-19
Computed gene(s)	Dmel\Graf

Collection Status	BDGP
Known Problems	none
Library	SD
Strain
Tissue Source	cultured cell-line
Vector	pOT2
Sequence Data of the Insert
5' Sequence
Total bases	610
GenBank	AI532880
5' sequence data	TCAAAGTAATATATATATATTGTGGTGCATTTAGAATACAATGAAACGAA ATATGCAATTGATGCTGAATAATATCGCGTAATTGATGCGAAACGCGCGA CACGACACGGCCAATTTGCGTTCTTCGATCAGAGCAACATTAGATATTGG GAAATTACTCATACACTAATGTTTACTCTGAAGGATTACCAATATGCATA ATCCACCAACTGATAAACAGTATTTGCATTGAATAGTAAATATATAAAAG GAGCGCAATTCCAGCTGTAAATGTTGCAAACACCGCAGTTTTCTCGAACT CAACCGACAAATCTACACAATATATCACAATATATCAGTCACTGCGAATG CACTTAGCTCTGTGGAAAGCAACATCGAAAGAACCTAAATAAAACTGAAA CTGAAACTGAAAATCACAGCAAATATTCAAATTCAATATTATTAAAAGCA AACGCAAACATCATGGGCGGCGGCAAAAATGTACGCCGCGGCTTGGAGCC TCTGGAGTTCGAAGAATGCATCGTCGACAGTCCGGACTTTCGCGAAAATT TAAATCGACATGAAAAGGAACTGGACCACACTAGTCATCAAATAAAACGA ATTATCAAAG
Library Information
Comments	The SD library was made from RNA extracted from Drosophila tissue culture cells. mRNA source: non-isogenic Schneider L2 cells, polyA+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
Library Synonyms
Reported As
Symbol Synonym	SD
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
External Crossreferences & Linkouts
Linkouts
	DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines • FBcl0277745
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 0 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]