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General Information
Symbol
Dmel\SD07723
Species
D. melanogaster
Name
SD07723
FlyBase ID
FBcl0279676
Feature type
Computed gene(s)
 
Collection Status
DGC gold BDGP
Known Problems
none
FlyBase assessment
    Library
    SD_cDNA
    • SD cDNA library of D. melanogaster, Schneider's Line-2 cells.
    Strain
      Vector
      Tissue Source
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      6,395
      NCBI
      Full Length sequence dataSequence Downloader
      CCACGACAGCGTGTATATTTAAAGTTAAACAAAATCACCAACGCGAATTGCATTCTACGATATTCAAACGGAACAGGAGT
      GATAAAGCTTTAACTTAAGCTCTATCCTAAGCAGTAAAACAAAAGTTTCAAGCACAATCTTTTGTTCCGTCCGGTCTGTG
      AAAAAAAAGAATTAATCTCAACTTTTATTTCTTATCTTGAAATGTAAGCATATGCCGAAGTCGTACAATACATATGTACA
      TTCAGCAGTAATTCATATTATATTGTATATTAGTATAAATCTGAATATTCTTTAAACGCCACCTGGCGGTTACTTTGGCA
      AGTGAGTTTGAATCCAACTTTTGGGCAAAGTAGCCCGGGTTCCTAGTGTGCTATGTCCTCCCAGCTGGAAAGCTTCACCT
      GCGGCCAACTGTCACCATTCGCCAGCTGGTGCAACAGATGCTCGGCCAGCTGGAAGGACACGCACGTCAATCTGGTGGAG
      GCGCAGGCGCGTTGGCAGGTAAACAAACACCTGTAGCACTCGCGGCGAGGTAAACAAGTGCAGGGCGCAGATGCGCCAAA
      TGTCAGGGCAAGCGCACTGGGTGGAAAGAAGAGTGCGGCAAATGGCCAAAAGCGAGAGACAAGGGTTGTCCCTTTTCGGC
      TAAGGTATGTTGGGCAAAAGAGCTTTAAGCTGGCCCAGAAACGTTAAAAGAAACGTCAAAACGGCCACGACAGTGTAGAG
      AGAAAAGTTTTCGCTGGCCAACAACACACACACACGGAAAAGTGCTAGGTCGCGCGATAAAACTGTGGAAAATTAATTTA
      GAAAATTGGAAAAACTTAGCAAAATACAATGTACAGTGCTTGAGTGACGGCGAGTATTGAGTAGCAACTGAAGGTATCCC
      CAGTGAAGACGCGGGTGGTATGCTACCGGATGGCCAGGGCAGGTGGCCAAAACCGAGCTAACGGGATATGTGGTGCAAAC
      GTGGTGCGTGCGTGTTGCCTGTGAGTGTGTGAATGTGTGTGTGTGCAGGTACAAAGTAGGCTAAGAAAATCGCAGAGCCT
      GGTATTTATGTCGCGCTCCCGCTGGATCGACCACCATCATCAGCAGTTACTAGATCATTGGACACCGAGGATAGCGCTGC
      TCAATCATCATGGCCTGGTCCCGTCTCCTGGCCTGGTGGCGCCTCTGCTTGGGCCTGCTCTCCGTTCTGGTCTGTTTGGC
      ACCGTGCGTCAATCTGCAGCCAGCCGAGAACATCAACTACAATCTCGTTCATTCGTGGGCCGACAAATTGGGCATGGAGC
      TGTTTCATCTGGGCGATTTCATCACCAGGCGCAAGGAGGTGCAAGAGAGTTTCAAGGATGCAAAGGTCGTTTCGCGCAAC
      GGCGCTTCTATTGTCGATTCGATGGCCAAGGAAATCGAAATGATGATGGACCTAAAAGTCAGTGCAGTGAGGCGAATCAT
      GGACACGGCTGAGAACACGGCGTTGTCGCATCAGAACGACATGGCGGACAAGATGTTCTCCTATTATAGTGCCAAGGAGA
      TGCTGGAGCCCGGCGATCCAGTCCCGCCCATTCCCACACCGGCTCCCGACATGGACAAGGATATAGGCGAGCCGCTGATC
      TACGTCCAGCCGAAGGTGGTGGTGTTGGAACCGCGTCCGGAATTCCACAATACTCCGGTCAACTTTAGCGTCAGTTCGGT
      CCACGTGCCAGTCAATGTGTTCGATCGAGCACCGGATGTGATAAAAGCAATTCAGTGGTCGGAGAATTTGGATCAGATAT
      TCCGTGACAATTACAAAAACGATCCGACATTGTCGTGGCAATTCTTTGGCAGTTCGACTGGCTTTATGCGTCAATTCCCT
      GCATCGAAGTGGCGAAAGGACGTACCGGTCGATCTATACGATTGCCGACTACGCTCCTGGTACATGGAGGCGGCGACGAG
      TCCGAAGGATATTGTTATCCTGATGGATGGATCCGGTTCAATGTTGGGACAGAGGCTAGATATCGCAAAGCATGTTGTCA
      ATACGATACTCGATACATTAGGTACAAATGACTTTGTGAACATCTTCACCTTTGATAAGGAAGTGAGCCCAGTCGTCCCA
      TGTTTCGAAGACACACTGATTCAAGCCAATCTGGGCAACATACGCGAACTGAAAGAAGGGATTGAACTGTTTAGACCCAA
      ATCGATCGCCAATTATACCGCTGCATTGACAAAGGCATTCGAGCTATTGGAAGAGACCAAGTTGAGTTCGCGCGGGGCGC
      AGTGCAATCAGGCGATCATGATCATTGGCGACGGGGCGCCGGAGAACAATCGCGAGGTCTTCGAGTTGCACAATTGGCGG
      GACCCGCCGTACAAGCCGGTCCGTGTGTTCACCTACCTGATTGGCAAGGAGGTGGCCAATTGGGATGATATACGGTGGAT
      GGCGTGCGAGAATCAGGGCTACTACGTCCACCTCAGCGACACCGCCGAGGTGCGCGAGATGGTCTTGAATTACATACCGG
      TTATGGCCAGGCCGCTCGTCCTGGGCAGGCACGATCATCCGGTGATTTGGTCGCAGGTCTATGCGGACATCGAGGACACG
      AAGCTATCCGACTATCTGTGGGACATTAATCAGTGCGAGTACCAGAAGGCCGATGTCCTCGAGTATTGGCAGGTGCACGA
      TCGGATGCTGGAGCCCAGCGAAATGCACCGCCGGAAGTACAGAAGGATGAAGGAGACCTGGAACCAGCCGGTGGACTCGA
      ATGTGTACCAATTTATGACCACCGTATCGATGCCCATCTACGATCGTCGCGAGAACGCGACTAGGATTGCGAATATATTG
      GGCGTGGCCGGCACGGATGTGCCCATCAATGAAATCAAAAAGCTGCTGTCGCCGTTCACGCTCGGCGTGAATGGCTATGC
      GTTTATAGTAACCAACAATGGTTATGTACTATTCCATCCAGACTTTCGTCCAATTTTCCAAGGCTACATATTAAAGCCAG
      CATACAATAGCGTTGACATGATTGAAGTCGAGCTATTGGATGACGATCGACCCGCCAGAGACTTTAATCCAGTGCTGATG
      ACGATAAGAGATTCTATAATCAATCAATCGACAGGCAGCAAATGGATGTTAGTCAAAAATCATTTCGATGAAATGAAACG
      GGTGGCACGCGTAAAGCGACAATACTACTGGACGGCCATCAAGAAGACGCCCTTTACTCTGGTCATTTCCTATCCGGAGC
      AATACGGCGTGAGTCACATGGACATCCGAGCTGACCAGGAGATCCATCGCATAAGCATAAAGGGCACAAACTTGCGCAGC
      GTTTTCAGCGGCAAGCGGTGGAAAATTCATCCCGACTGGCTATTTTGTAAGCACAGCAACAGGACATTCAAAACGCCAGA
      GATCGAACTGCTGTACTTTCTCGAGCGTATGTCTGAGCCTGGCTGGCGATGGCCGGGTAGTCGGAGTGCTATGCCCCCGG
      AGCACGCGGCCGCCATGTTTTGCGATCGGCAGCTAATGCAGGCTCTGGTCTTCGATGCCCGAGTCACCGGGTGGTTCTCC
      AACAATACCAGCTTTAACTCCAAGGACGACAAAGGAAACGAATTCAAGCAGCGTTTTGGCGTCACCGTCGCCTTTTTGGC
      CACCCACAGTGGTCTCACCCGCTGGCATGAGTTCCACTCGAATGCTGCCGAGGAATCCGGAGTCGGCGAGACTTTTAGCC
      AAAATAACACGCGGGCCATCGATGAGATTTGGTACAAACGCGCCGTGGACCAACATTTTGTGCGGGAGGAGAGCTTCGTT
      TACTCCGTGCCCTTCGATGCGGGTGAAAGCAACTCAGAGATCCTGGTGACCGCCAGTCATGCCGTTTTCCACAATGAGGG
      CGGCAAGACTGCACCGGCGGCTGTTGTGGGCTTCCAGTTTCAGCATTCGGCGCTCTACAAGCTCTTCCACAACATCACCG
      GCAATGCCTGTGCCGTGGATGACAAAGACTGCTATATACTGGACAACAATGGCTATGTGATCATTTCGACGAGGGTCCAC
      GAGACGGGTCGATTCTTTGGCGAGGTGAACGGAGCGATCATGAAGCGACTACTGGAGGAGAATGTCTACAGGCAGGTCAC
      TGTCTACGATTACCAGGCGGTGTGCTTCGAGTCCAAGAACGATAACAACGCCTCCAGCATGCTGCTTTCGCCCCTGTTCC
      ATCTGCTGCGCGTGGGCAAATGGCTGCTGCATACGGCGCTGTGGTATATTGTACAACTGTTGCAGTGGGCGCCCGGTGTG
      TCCAGCCACTATGCGGATATGTATGGTGACTCCAACGACACGGAGCCACCGCCGCCGGAGCCGCATCCGGATCACCATGC
      TCGCAACGGCAATGGGCATGGGAAGAAGGACGATGACCACTGGCTGCGCTACTTGACACTCCATCGCACCCGGCTCAAGC
      CGTGCGACATGAAGCGCGATCTGTACACGCTGTTCAATGAAAAGGACAACGTTGTCTACAATATGACAGCCCATGCATGT
      GAGCGGCCATTTGTCGTCCTGCCCATCCCGTTCAGCAACCTCATCCTGCTGGTCATCGACCAGCTGTGTCCCAGGGACGG
      CTCAGTCGTCCTCACGGTGAATCCGCAGCCGATCGACTATCATCTGTCGGTCAACGATTCGTTGGCATGCTACAAGCAGG
      CTCGCGAGTTCAACCGCATGCGACCACACAGCTGCATCAGTCGGCACGCGAACGAGAGCGGCATTAAGCTGTGCGGAAAG
      GCCTGCTCCGTGTACGCCAACCTGGGGCTGCTGCTCCTCTGTCACATACTGAGTCGTTGGCTGTAACGGCGGAAGATGGA
      TTTAGCTTAGATCTAGGCAATATGTTTTTGTATTATATACGAAGGGCAATCGAGGAGGTGGGAAAATTTTTTACTCTAAC
      GCTTGAGCGCAGTTCAATATTTTTGGTCACCTAGAGAAACTTTTCCAAATTAGTTCTTAAGTAGCTAAATACGAGTATCC
      GTATCCGCGACCGAATCCGGAAGCGGATCCGCATCAGAGAACGCGGACAGTGTGTGCCGCCTATGACGGACTGGGCGCAG
      GGCTTATTGTAGACCTTCCAATAGACAGGACAAAAACATATATTTACTGCATTAACATATGAATATAAATATTATATATT
      GTATAACTGAAAATTCCAACAACGGCCTTAAACGACTTGCAACCTTCAAGTAAGCTGAATGCAAAGCGCTGTCATCCAGG
      CGACAAAACTCATGGCACACACTGTACCCAATGCCGACTACTGCCCATGGTTATGCGTGTGTGTTTGTGTGTGTGTGTGT
      GTGTGTGTGTGTGTGAGTGAGTGAGTGTGTGCGTGGTGTGTGTGTGCATGTGTGAGAGTTAGTCCTAATCTAAGAAGGCC
      ACATTTGTAAGTAACACCTAGCTACGAGTTTATGTTTAAAGAACGGATCAAGAAACCTATCGGTAAAGGCGAATAATGGA
      ACCATTTAATTATATATACACACACTCATACGATAATACGATATATACCTACGAGTACGCCCGGTACTGTACATGCTAAC
      TAATTTAAGTAGCTTTTGTATATTTGCTTAGCCTAGCCCAGAGAAAAACTAACCGAAAGTTCAGCCTCGGAGCCTTGGAA
      AACTACATACTTTTATAGACATTCGTTAAAAAGGACATTGTAGGCCACTCAAGAACAAGCAAAACCAAAAAGAGATCCGA
      TTTAAAGGCATTGTTTTTATATACACTGATGATGACGATACCTAGCATTACCCACAAGTACCTACATACATATATGAGAA
      ATACTTTTTATTACTAACTACTTGATACGAGCCACAGAACTGAGACGATTGAGTAAACGCAGCAGCATCTAGCGACTAAA
      TTATTAATAATACTACACTTAAACCATTGACTAGTTGAAATTTACCATAACAAACTATCGTACATCTAGACAAACAAACT
      CTCAGCCGAAAGGATATCTCCTCCGATCGGAACAGAACTCGCTAAGCTTTAGACACCCCACATTTCCCCATGGTAGTGTA
      ATCAACTTGTGCCATTCGAACTGCCTAGTATTTGTTATTAACTATTTAATATGGAATCGGAAGTACGTTTAACCCCCTTG
      CAGGTTGTCTCACCACATCCCTACTCATTTTGTACATTTCGATTGAAAGTAGGGTAAAGCAAATATCAAACTATGCCAAG
      CTTGCAGGCCTCTTTACCAGAACATTTTAGCCCGATCTCAAGTTTAAACTCTTCTCACAGACACAAACGAAACAAAGGAT
      ACAAACACACTCGCTGGCAGCCACAATTGTACTACATACATAAACATATTTCGAATTCAATCAAATACAAGTAAAACCAT
      TTGTCTAATGTGCTAAAAGAAGAAACTGATAATAAACACAAGATAATTTCGAAAATCAAAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Library: SD_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.

      Sample preparation

      For the SD cDNA library, Schneider L2 cells were collected.

      Protocol

      RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

      Comment
      Reported As
      Symbol Synonyms
      SD
      SD_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)