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General Information
Symbol
Dmel\SD10012
Species
D. melanogaster
Name
SD10012
FlyBase ID
FBcl0281004
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI05269
Library
SD_cDNA
  • SD cDNA library of D. melanogaster, Schneider's Line-2 cells.
Strain
    Vector
    Tissue Source
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    4,229
    NCBI
    Full Length sequence dataSequence Downloader
    CACTCGTCGGCTGCAGACGTCTTCTTGAGCACTTCCCAGTGCGAATAGAGCCAGAGGAAATATAGATATATATATACATA
    TATAAAGTCTTCAGACGATCGCGAGTTTCATCAGTTTGCCAAGTGTCCACGGAAGATCGGCACGTGCTAAGCTTAATTGC
    TATAGCGAGTATAGAGATACTTTACTGCAGTATCACCTCATAGACACAATGGTCAAGAAGGAGGAATCACGGCTGCCACA
    AGCTGGTGACTTTCAGCTTAAGGAAGGATCAGTCGTGGATGCGACCCGAAAGTACAGTTACTTTGATCTGTTCAGATACT
    CAACCAGATGCGAGCGCTTCCTGTTGGTAGTCAGTCTGCTGGTGGCCACCGCTGCGTCCGCATTTATTCCCTACTTCATG
    ATTATCTACGGAGAGTTCACATCCCTGCTCGTGGACCGAACAGTCGGCGTGGGCACATCCTCGCCAGCCTTTGCTCTGCC
    CATGTTTGGTGGCGGTCAGCAATTGACAAATGCCAGCAAAGAGGAGAACAACCAGGCGATCATTGATGATGCGACTGCCT
    TTGGCATTGGCAGTCTGGTGGGATCTGTGGCCATGTTCCTGCTCATCACACTGGCCATCGACTTGGCCAACCGGATAGCC
    CTCAACCAAATCGATCGCATCCGAAAGCTCTTCCTGGAGGCAATGCTGCGACAGGACATCGCCTGGTACGACACCAGTTC
    GGGCTCCAACTTCGCCAGCAAAATGACAGAAGACCTGGACAAACTGAAGGAAGGCATTGGCGAGAAAATAGTGATTGTGG
    TATTCCTGATCATGACCTTTGTGATTGGAATAGTGTCTGCTTTCGTCTACGGATGGAAGCTCACCCTGGTCGTTCTAAGT
    TGTGTGCCCTTCATCATTGCAGCCACCAGTGTGGTGGCTCGACTCCAGGGATCGCTGGCCGAGAAGGAGCTGAAGTCCTA
    CTCCGATGCAGCCAACGTGGTCGAGGAGGTATTCAGTGGAATTCGCACAGTGTTCGCATTCAGTGGTCAGGAGAAGGAGA
    AGGAACGCTTTGGCAAGCTGCTCATTCCCGCAGAGAATACGGGTCGCAAGAAGGGTCTATACTCGGGCATGGGGAACGCT
    CTGTCCTGGCTGATCATCTACCTGTGCATGGCCCTTGCCATCTGGTATGGTGTGACGCTCATTCTGGACGAGCGTGATCT
    TCCCGATCGCGTTTATACTCCGGCCGTTCTGGTCATTGTCCTGTTCGCCGTGATCATGGGTGCCCAGAATCTCGGTTTTG
    CCTCTCCACACGTGGAAGCCATTGCTGTGGCCACGGCAGCTGGCCAGACCCTGTTCAACATCATCGATCGTCCGTCGCAG
    GTGGATCCAATGGACGAGAAGGGAAACAGGCCGGAGAATACCGCTGGACACATTCGTTTCGAGGGCATACGCTTCCGTTA
    CCCCGCCCGTCCGGATGTGGAGATCCTCAAGGGTCTGACCGTCGATGTGCTTCCGGGTCAGACGGTGGCCTTCGTGGGCG
    CATCTGGGTGTGGCAAGAGCACCCTCATCCAACTGATGCAGCGGTTCTACGACCCCGAAGCGGGCAGTGTCAAACTGGAT
    GGACGGGATCTTCGAACACTGAATGTGGGCTGGTTGCGTTCGCAGATCGGAGTGGTGGGCCAGGAACCGGTTCTCTTCGC
    CACCACCATTGGCGAGAATATCCGATACGGAAGACCATCGGCCACTCAGGCGGATATAGAGAAGGCGGCGCGAGCGGCCA
    ACTGTCATGATTTCATCACTCGCCTACCCAAGGGCTACGACACCCAGGTGGGCGAGAAGGGCGCTCAGATTTCTGGTGGC
    CAGAAGCAGCGGATTGCCATTGCCCGGGCTTTGGTTCGACAGCCCCAAGTGCTTCTTCTCGACGAGGCCACCTCTGCCCT
    GGATCCCACCTCTGAGAAGCGGGTGCAAAGTGCCCTAGAACTGGCCAGCCAGGGACCAACCACTCTAGTTGTGGCCCACC
    GACTGTCCACCATCACCAATGCCGACAAGATTGTCTTCCTCAAGGACGGTGTAGTGGCGGAGCAGGGTACGCACGAGGAG
    CTCATGGAGCGACGTGGTCTCTACTGCGAACTGGTGAGCATCACCCAGCGGAAGGAGGCCACGGAGGCGGACGAGGGAGC
    GGTTGCCGGGCGACCTCTGCAGAAGTCGCAGAACCTGTCTGACGAGGAAACCGATGATGACGAGGAGGATGAAGAGGAGG
    ACGAAGAGCCGGAGCTGCAGACTTCGGGCAGCTCCAGGGACAGCGGATTCAGAGCCAGCACGCGACGCAAGCGTCGCTCG
    CAGCGTCGCAAGAAGAAGAAGGACAAGGAAGTGGTATCCAAGGTGTCCTTCACTCAACTAATGAAGCTTAATTCTCCGGA
    GTGGCGTTTCATTGTCGTCGGAGGCATTGCATCCGTAATGCATGGCGCCACATTCCCGCTTTGGGGTCTCTTCTTTGGCG
    ACTTCTTTGGAATCCTCTCGGACGGTGACGATGACGTTGTGCGAGCCGAGGTCCTAAAAATCTCCATGATCTTTGTGGGC
    ATTGGCCTGATGGCCGGATTGGGTAACATGCTCCAGACCTACATGTTCACAACGGCCGGCGTGAAGATGACAACGCGATT
    GCGTAAACGGGCTTTTGGAACGATTATTGGTCAGGATATTGCCTACTTCGATGACGAGCGGAATTCAGTGGGAGCACTGT
    GCTCCCGGCTGGCCAGCGATTGTTCCAACGTTCAGGGTGCAACCGGAGCTCGTGTGGGCACAATGCTCCAGGCCGTGGCC
    ACTCTTGTGGTCGGAATGGTCGTCGGTTTCGTTTTCTCCTGGCAACAGACGCTCCTCACACTGGTCACCCTGCCACTGGT
    CTGTTTGTCCGTCTACTTGGAGGGTCGTTTTATCATGAAGAGCGCCCAAAAGGCTAAGGCGTCGATCGAGGAGGCTTCTC
    AAGTGGCAGTCGAAGCTATTACCAATATCCGAACGGTCAATGGTCTTTGCCTAGAGCGCCAGGTCTTGGATCAGTACGTT
    CAGCAGATCGATCGCGTGGATGTCGCCTGCAGGCGCAAGGTTCGCTTCCGTGGTCTGGTTTTCGCTCTGGGACAGGCGGC
    TCCTTTCCTGGCTTACGGCATCTCAATGTACTATGGTGGTATTCTGGTGGCAGAGGAGCGAATGAACTATGAGGACATCA
    TCAAGGTGGCCGAGGCTCTGATCTTTGGCTCCTGGATGTTGGGACAGGCCCTGGCCTACGCGCCAAATGTAAACGATGCT
    ATTCTGTCCGCCGGACGCCTAATGGATCTCTTCAAGCGCACCTCCACGCAGCCCAATCCGCCACAGAGTCCCTACAATAC
    AGTTGAGAAATCCGAGGGAGACATTGTCTATGAAAACGTGGGCTTCGAATATCCCACCAGGAAGGGCACTCCCATTCTGC
    AGGGCCTAAATCTCACCATCAAGAAATCGACGACGGTAGCTCTGGTGGGTCCATCTGGTTCCGGCAAGTCCACCTGCGTG
    CAACTGCTGCTTCGCTACTACGATCCCGTATCGGGATCCGTGAATCTGAGCGGAGTGCCGAGCACGGAATTCCCGTTGGA
    CACACTGCGCTCCAAGCTGGGCCTGGTCTCGCAGGAACCCGTGCTCTTCGATCGCACCATTGCCGAGAACATAGCTTATG
    GCAACAATTTCCGTGACGATGTGTCCATGCAGGAGATTATTGAGGCGGCCAAGAAGTCGAATATCCACAACTTTATAAGT
    GCTTTGCCCCAAGGCTACGACACCCGACTGGGCAAGACCTCGCAGCTCTCTGGAGGTCAGAAGCAGCGCATCGCCATCGC
    TCGAGCCCTGGTCAGAAACCCGAAGATACTCATCCTGGACGAGGCAACCTCTGCGCTGGACTTGGAGAGCGAGAAGGTGG
    TGCAACAGGCGCTGGATGAGGCGAGATCAGGCCGCACATGCCTGACGATTGCGCATCGTCTGACCACCGTGCGGAATGCG
    GATCTCATCTGTGTGCTAAAACGCGGCGTGGTAGTGGAGCACGGCACCCACGATGAGTTGATGGCACTGAACAAAATCTA
    CGCCAATCTCTATCTGATGCAGCAGGTGTCGGGCTAACACAGCGAACTGGAGTGATAATTGTTAGGCTTTAATTTAGTAT
    GTACACTAGGCTAATTGAACATGTTGAAGTATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
    
    Library Information (1)
    Library: SD_cDNA
    Description

    Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.

    Sample preparation

    For the SD cDNA library, Schneider L2 cells were collected.

    Protocol

    RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).

    Comment
    Reported As
    Symbol Synonyms
    SD
    SD_cDNA
    References (1)
    Review (1)
    Rubin et al., 2000, Science 287(5461): 2222--2224
    A Drosophila complementary DNA resource. [FBrf0127297]
    External Crossreferences and Linkouts ( 2 )
    Crossreferences
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Linkouts
    DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)