Clone Dmel\SD04011

General Information
Symbol	Dmel\SD04011	Species	D. melanogaster
Name	SD04011	FlyBase ID	FBcl0285757
Feature type	cDNA_clone	Created / Updated	2005-11-02/2006-08-19
Computed gene(s)	Dmel\RhoGAP16F

Collection Status	BDGP
Known Problems	none
Library	SD
Strain
Tissue Source	cultured cell-line
Vector	pOT2
Sequence Data of the Insert
Full Length Sequence
Total bases	1970
GenBank	AY052089
Full Length sequence data	GCGCCGCGGGATTCGTGGCACACGAGAGACGCCAGCCCGGAAAGACCCTG ATGAATTGCTTTCGCGATCGCTCCACGACGTCGTCGTCATCGTCGCAGGC CCAACACGCCTATGTGCGCGGCCACAGCGTCACCTCGAGCACTCTGTTTC GCCTGGAGGGCAGTGGTGGCTCCAATTGCACCTTGGACAAGGCGGGCAAG CAGGATACCGGGCGGCCCGAGGCGGTCGTCCTACTCAGAGAGCTGAAAAC CAGACCCAACAAGCTGGCTCTGCTCAAGAGCAGCAGCAGCAAGGATCTGA CCCGCCTGTTCCTGGACGATTCAACCAACACAGCCGTGCTGGTGTCCAAC ACCAGTATAGATGTCTGCTCCTCAAATTCCAGCTCTGGCGGCAGTCCGGC GGTGGGCAGGTCCAAGTCCGGCAGGGATCGCCGCAATGGACTGGAGTGCT GCAGCAGCTGCTCCAAGCGTTGGCACGATCTCAAGCAGCGTATGCACACT CTGGAGCAGGATCTGCTCGTCCAGACCACATACAGCCAAGAGCTGGAGCA GAAGGTGGGCGAGATGAGTCGCCAGTTGACGGAACTGCTTCAGGAACGCG ACAGGGAGAGGGATAAGGATAAGGCGCGCTTTGCAGCCGCAGGCGGCGTT GCTGCGCCTGGCAAGCGGACTGCTCACGGCAGTCCCAATGTCCGACCCTC GCGCCTGGTGGCCTGGATGAACCTGTCCAACTGGTTCAAGAGCAGACCCA GCGTGGAAACGCTGCGGGAACAGCGCATCTTCTTCGACGAGCCCTGCTTC GATACGGAACTGGAGATGGTCCTCAAGCACGACCAGCACCGCACTGTGCC GCGCATCGTTGTGGATTGCTGCGATCTAATCGAGCAAAAGTACCGCAGAT CTACGCAGCCCATCGAGGGCATCTATCGGCAGTGCGGCGACTACAATAAA ATCCAAACGCTGCGCTTCAGCATCGACGCCAACGACTACGATTCACTGCG TCAGCCGGACGTCGATATACACACGTTGACCGGTGTGCTTAAGCTCTTCC TGCGCGAAATCAAGAGTCCGTTGGTCAGTGTCAACGAGGCCAAGACCTTC ATTGGACAGCCCAATCAGTGGTTGTTAACCGATCTGTCTGCGAAGTTGGA CTCCCTGAAAAGACTGATTCGTTCGCTGCCGGAGAGCAACCGGGACACCA TGGACTACATCTTCGGTCACTTCAATAGAATAACCAAGGTGCCGCTGCAG CAGATCAGCGCTGAAACGCTGTCCATATCGGTGACGCCGTCGATCTTTCA CACGGTGCCACAGGGCGTCCACATGCAGGACATCCAGCAACTTTTGCGCG AGGGCGAAACGCTGGCCGACTGCGTCAAGCTGATGATCGAGTACCAGGGG CGCATATTCGAGTAAGTGCGAGCCCCGCTGAGGAGGAACAGCCCCAGTCT CCCAGGTGATGGTCCATTTGGTGTCCGGGCGCCCGCCGCTACTGTTACCC ACAAAACCCGAATCCCAGCCGCTTACGTCTCATCCAAACCGCTCACCTAC TGTGCCGTTGTGTCGAACGCTTTGCTAACCACTCGAAATCCGAAATCCTC AACCCGAAACCGAACACCCCACTGTACCTGCCACACCTCTATACCCATTC CCGATTCGCTCCCGAATCTGTTTCCAATCCCGATCACATCGTCTACAGAT GCACCGCCGATCGCCGCCGCCTGAGCATGCGCAACCTAAAGAAGACTCTG TCGCAGCCGGACCTGCTCTTTCACAATCTATTCTGAGCTGGCCCAATGAC ATCCAATGGATCCTTCATCCCAGAGAGAGCACTGTACATATATGGAATGA CTTTACGCCCCGCTAGCAATTGTGTCCTAAGATTACTTTTTATATTCTTA CTTTATCAACTAACTTTTGTACTTATGTTTACTAATATAAACACTAAAAC TAAAAAAAAAAAAAAAAAAA
Library Information
Comments	The SD library was made from RNA extracted from Drosophila tissue culture cells. mRNA source: non-isogenic Schneider L2 cells, polyA+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
Library Synonyms
Reported As
Symbol Synonym
Library References
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]
Synonyms & Secondary IDs ( 0 )
Reported As
Symbol Synonym
Secondary FlyBase IDs

References ( 0 )
Review	Rubin et al., 2000, Science 287(5461): 2222--2224 A Drosophila complementary DNA resource. [FBrf0127297]