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General Information
Symbol
Dmel\AT01906
Species
D. melanogaster
Name
AT01906
FlyBase ID
FBcl0294705
Feature type
Computed gene(s)
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    AT_cDNA
    • AT cDNA library of D. melanogaster, Oregon-R strain, adult male, testis and seminal vesicle.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Total bases
      4,201
      NCBI
      Full Length sequence dataSequence Downloader
      CTTCTCGTTGTCCTGTCTTGTCCGTCTTGCTGTGCGTGTCCGAAATGAAATGATTTATTTGATTAGACAATCCCTAGGCT
      GCGATTGCGTTTCGCGATGGTTTCAGCAATAGCGATGGCGATGGTGATTGCCAAGTGCGAAGCCCCCTAAGTCCGGCCTG
      TCATCGCATTCGCATTCACCATCGCAGACGCGTTTTTATGGCTCTTTTAGTGCTGGTCATGAAATTGGGGCAGGAGTGAT
      AGCTGCCATAGGCAATCTCATTACTGGCGACATTTCTGCGGCATTCGAGGCTTGTTTAATCGCGTTTCCACTTCGCAGAA
      CAATCACATGTTCAACTCCCAGCTGCGCAAGCAATCCGTCCAGTTCCACGGCCAGGATGGATTTGGCTCCGGTTATGGTT
      CCAGGATCAGTATCGCTGCAACGCAATCTGACTCTCGTCGGCGGAGGAGAAGGGGCGTGGGAGGCACCAGCGGAGGGCAC
      CTCTTTCCGGAGGCGATCTCGCAGTACGCGCCACAAACCTACGACGCCGTGTGGGCCATCGCCCTGGCCTTGAGAGCCGC
      TGAGGAGCACTGGCGGCGAAACGAGGAGCAGTCGAAGCTGGACGGATTTGATTACACCCGCAGCGACATGGCATGGGAGT
      TCCTGCAGCAAATGGGCAAGCTCCACTTCCTGGGAGTGTCGGGCCCCGTTTCCTTCAGCGGCCCAGATCGCGTTGGCACC
      ACTGCCTTCTATCAAATCCAGCGCGGTTTGCTGGAACCGGTGGCCCTCTACTATCCGGCCACGGATGCCCTGGACTTCCG
      GTGTCCCCGCTGCCGGCCGGTGAAGTGGCACAGCGGGCAGGTACCCATCGCCAAGCGGGTGTTCAAGCTGCGGGTGGCGA
      CCATCGCTCCACTGGCCTTCTACACCATCGCCACCCTCTCCAGCGTGGGAATCGCTCTGGCCATCGCCTTCCTGGCGTTC
      AATCTGCACTTTCGGAAGCTGAAGGCAATTAAACTTTCCAGCCCGAAGCTGAGCAACATCACCGCAGTGGGCTGCATCTT
      TGTGTACGCCACCGTCATCCTTTTGGGTCTGGACCACTCGACGCTGCCCTCGGCGGAGGACTCTTTCGCAACGGTCTGCA
      CGGCCCGCGTCTATCTGCTCTCCGCCGGATTCTCGTTGGCCTTTGGATCGATGTTTGCCAAGACCTACAGAGTGCATCGG
      ATATTCACTCGTACCGGCAGCGTTTTCAAGGACAAGATGCTGCAGGACATTCAACTGATCTTGCTCGTCGGCGGATTGCT
      TCTGGTGGATGCGCTGCTCGTAACCCTTTGGGTGGTCACCGATCCAATGGAGCGCCATCTTCACAACCTGACGCTCGAGA
      TCAGTGCGACTGATAGAAGTGTCGTTTACCAGCCTCAGGTTGAAGTTTGCCGTTCGCAGCACACGCAAACGTGGTTGAGT
      GTCCTGTACGCCTACAAAGGCCTTCTTCTTGTGGTGGGTGTCTATATGGCCTGGGAGACGCGCCACGTAAAAATACCTGC
      TCTCAATGACTCGCAGTACATCGGAGTGTCTGTATACAGTGTGGTCATCACCAGCGCCATCGTCGTGGTGCTGGCCAACT
      TGATTTCGGAGCGAGTCACCCTGGCCTTCATCACAATCACAGCTCTGATTTTAACCAGCACCACTGCAACCCTTTTGTCT
      GCTTTTCATCCCAAAACTCCATGATATTTGGGCAAGAAACGATATTATCGATCCGGTTATCCACAGTATGGGCCTTAAGA
      TGGAGTGCAACACACGCCGATTCGTGGTCGATGATCGCCGAGAACTGCAGTATCGAGTGGAGGTGCAAAACAGGGTCTAT
      AAGAAGGAAATCCAGGCTCTGGACGCCGAGATTCGAAAGCTGGAGAGGCTACTCGAGTCGGGACTAACCACCACCTCCAC
      CACAACTTCGTCGTCCACATCACTCTTAACTGGGGGAGGTCATCTAAAGCCAGAACTGACGGTAACCAGTGGCATCTCGC
      AGACTCCGGCTGCAAGTAAAAACAGAACTCCAAGTATCTCGGGAATACTGCCCAATCTCCTGCTTTCCGTGCTGCCTCCT
      GTGATTCCACGGGCCAGTTGGCCGTCAGCAGAGTACATGCAGATCCCGATGAGGCGTTCTGTTACCTTTGCCTCCCAGCC
      CCAATTAGAGGAGGCCTGCCTGCCTGCACAGGACTTGATTAACCTCCGTTTAGCCCACCAGCAGGCCACGGAGGCTAAGA
      CGGGCTTGATAAACCGATTACGAGGGATATTTTCTCGCACCACTTCGAGCAACAAGGGATCCACCGCCAGCTTGGCGGAC
      CAAAAGGGTCTGAAGGCGGCCTTTAAATCGCACATGGGACTGTTCACCCGCCTGATTCCCTCCTCTCAAACGGCGTCCTG
      CAATGCCATATACAATAATCCAAATCAGGATTCCATTCCCTCAGAGGCGTCCTCCCACCCGAATGGTAACCACCTAAAGC
      CCATCCATAGGGGTTCATTGACCAAAAGCGGTACTCACCTGGATCACCTTACCAAGGATCCGAATTTCCTGCCTATCCCC
      ACTATTTCTGGCGGTGAACAGGGGGACCAAACGTTGGGTGGAAAGTATGTGAAACTGCTGGAGACCAAGGTGAACTTCCA
      ATTGCCCAGCAACCGGAGACCTTCGGTGGTGCAGCAGCCACCCAGTTTAAGGGAAAGGGTAAGGGGTTCGCCACGCTTTC
      CACACCGCATCCTGCCGCCCACTTGCAGTCTCAGCGCCCTGGCCGAATCCGAGGACCGTCCCGGAGATAGCACCTCTATC
      TTGGGCAGCTGCAAGTCCATACCTCGCATTTCGCTGCAGCAGGCCACCAGTGGAGGCACCTGGAAATCGATGGAAACAGC
      GGGCAAGTCGAGGCTTTCCCTCGGCGATTCCCAGGAAGAGGAGCAGCAGGCGCCTGCGAATGGCACCGAATAAATAAGAT
      GCCCTGTCCATTGTCCACTCTGTACACGGTCCACACTTCAAATCCAAGTCCAAGTCCTCGTCAATTAGAAGCGAAACACC
      AAGCAATTATTTTACGACACGCCCACAAAATCTGCCCCAGAATAGCGCGTGTGCAACTGACCAATCATCAGCTTAAAGCA
      ATCAAATGCATTAGGGTAAAACGCTAGATAAGTCACGATACGAACCTCCCCCTCCCTCCTCAAGTCAAGTTCACATAGCT
      AGTCATTTTCCTGAACTTAGCAGAATCAACAACACGGAGAAAATTGCAGGCACATTGTGCTCTGCCTAGAAGTAGTCAAA
      ATTTACAATGTAAACTAAGAAATTTGTGCAATGATAGAATTTATCTTGATTCGCGAGTTTTCGGTATCAGCAAAGTTTTC
      TAAAAACTACCAGTATCAGTATTCGCCTCGGGGTGCCAAATGTTTACTTCTTGATAATCATTTCTTAAGACCTAGTGTAG
      TGTTCCTTTACTAAGCATGCATATTATTTCCTTAAGTATTTCGGTGAAAAAAGATGATAGCTCTTTAAATGGGTCTATAA
      AAATGTTGATTCCATTTTTTTCGTTTTAATTGATAAGATAACATCGCGGTCGTTTAAGCTTAGTTTTTGACCAAATCGCA
      TCTCTCTGTGTAGAACCTACTCCGCAATGTGATTGACCCTGGGCCGCGTAGGACCATCATCTAGGTTAAGTAGATGCGCT
      TGTCGGCCACTTTCAGTTTCCCAGCCAGTTGTGATTAACCCGCTCCTTAACCCCTTGCCACTAATCATCCTCCTAGTCGT
      AAGTAGCTGCATTAGCGTTTGTTTTCTTGTTGTTGTGCCGCCGCAGGACGGCTGTATCTGCGGTGTATCTACAGTGTATC
      CAGTGAGCAATACTAAGAATCGTACTTAAAGTTAGAACCTAGCATCAGCCAAGATCCAAAACCAGAACCAAAGTAGTCAG
      AGTAGAGTAGGGGTTGGCAGCCAGTGAGTATGATTAATCATCGCCGCCTAGCCTTGAATCATGTAAGAGTCCAGAGATGT
      TTGGGGCGAAAGCCAGAACACTTGAGAACTGCTCAAGACTAAGTCGTAAGTTTTAGCATTGTAATCCCAATCGACTTCGA
      GCCACACTCCAACAATCCAAAGTCCACCTTCCCACCCACTCGGATAACTTGAATAAACAGAAAAGTCTAAGCAGAAATGC
      CAAGCCAGAATAATGATTAATTAAAAAAAAAAAAAAAAAAA
      
      Library Information (1)
      Library: AT_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult male testes and seminal vesicles.

      Sample preparation

      Adult male testes and seminal vesicles were hand dissected from 0-3 day old non-isogenic Oregon-R males from a population cage.

      Protocol

      RNA was extracted from dissected testis and poly(A)+ selected twice (kindly provided by J. Pringle and M. Fuller). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; cDNA size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned directly into EcoRI/XhoI-digested pOTB7 plasmid. cDNAs were transformed into DH5-alpha strain (Gibco/BRL), or for AT121nn clones, into DH5-alpha TonA.

      Comment
      Reported As
      Symbol Synonyms
      References (1)
      Research paper (1)
      Stapleton et al., 2002, Genome Res. 12(8): 1294--1300
      The Drosophila Gene Collection: identification of putative full-length cDNAs for 70% of D. melanogaster genes. [FBrf0152058]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)