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General Information
Symbol
Dmel\SD04426
Species
D. melanogaster
Name
SD04426
FlyBase ID
FBcl0352058
Feature type
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    SD_cDNA
    • SD cDNA library of D. melanogaster, Schneider's Line-2 cells.
    Strain
      Vector
      Tissue Source
      Sequence Data of the Insert
      5' Sequence
      Total bases
      598
      NCBI
      5' sequence dataSequence Downloader
      ATTTCGTACGGTGTGCGTCGTAACAACAAGCAGCGTCTGAAAAGTTTTGTGAATTTCCAATTCTATACAAAGCAAAGTGA
      AAATATCTGTATTTTTACCTTTATTCTGTGAATAGAACGAAAAACATACATACAAGATGCCAGAAGAAGCAGAGACCTTT
      GCATTCCAGGCTGAGATTGCTCAGCTGATGTCCCTGATCATCAACACATTCTACTCGAACAAGGAGATTTTCCTGCGCGA
      GTTGATCTCGAACGCTTCCGATGCCCTGGACAAGATCCGCTATGAGTCCCTTACTGACCCCAGCAAGCTGGACTCTGGCA
      AGGAGCTGTACATCAAGCTGATCCCTAACAAGACGGCTGGTACTCTGACCATCATTGATACCGGTATCGGTATGACCAAG
      TCCGACCTGGTCAACAACTTGGGAACCATCGCCAAGTCCGGAACCAAGGCCTTCATGGAGGCTCTGCAGGCTGGTGCCGA
      CATTTCCATGATCGGTCAGTTCGGTGTGGGTTTCTACTCCGCCTACCTGGTCGCCGACAAGGTGACTGTCACCTCCAAGA
      ACAACGATGACGAGCAGTACGTGTGGGAGTCCTCTGCC
      
      Library Information (1)
      Library: SD_cDNA
      Description
      Cloned cDNAs prepared from polyadenylated RNA isolated from cultured cells; cell line used was not derived the iso-1 sequenced strain.
      Sample preparation
      For the SD cDNA library, Schneider L2 cells were collected.
      Protocol
      RNA was poly(A)+ selected once. oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~300,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
      Comment
      Reported As
      Symbol Synonyms
      SD
      SD_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)