FB2019_05, released Oct 15, 2019

Clone: Dmel\LP14927

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General Information
Symbol
Dmel\LP14927
Species
D. melanogaster
Name
LP14927
FlyBase ID
FBcl0352164
Feature type
cDNA_clone
Computed gene(s)
Dmel\Hsp83
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    LP_cDNA
    • LP cDNA library of D. melanogaster, iso-1 strain, larva and pupa.
    Strain
      Vector
      pOT2
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      613
      NCBI
      CK658186
      5' sequence dataSequence Downloader
      CCAAGTCCGACCTCGTCAACAACTTGGGAACCATTGCCAAGTCCGGAACCAAGGCCTTCATGGAGGCTCTGCAGGCTGGT
GCCGACATTTCCATGATCGGTCAGTTCGGTGTGGGTTTCTACTCCGCCTACCTGGTCGCCGACAAGGTGACTGTCACCTC
CAAGAACAACGATGACGAGCAGTACGTGTGGGAGTCCTCTGCCGGAGGCTCTTTCACAGTCCGTGCCGACAACTCTGAGC
CCCTGGGCCGTGGCACCAAGATCGTGCTGTACATCAAGGAGGACCAGACCGACTATCTGGAGGAGAGCAAGATCAAGGAG
ATTGTTAACAAGCACTCCCAGTTCATTGGCTACCCCATCAAGCTGCTCGTAGAGAAGGAGCGCGAGAAGGAGGTCAGCGA
CGATGAGGCTGATGATGAGAAGAAGGAAGGTGATGAGAAGAAGGAGATGGAGACTGATGAGCCCAAAATCGAGGATGTTG
GCGAGGATGAGGATGCCGACAAGAAGGACAAGGATGCCAAGAAGAAGAAGACCATCAAGGAGAAGTACACTGAGGATGAG
GAGCTGAACAAGACCAAGCCCATCTGGACCCGCAATCCCGATGATATCTCCCA
      Library Information (1)
      Library: LP_cDNA
      Description
      Cloned cDNAs prepared from polyadenylated RNA isolated from larvae and early pupae.
      (Rubin et al., 2000)
      Sample preparation
      Larvae and early pupae at various stages of development were collected from an isogenic y; cn bw sp (iso-1) strain.
      Protocol
      RNA was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested pOT2 plasmid. ~500,000 colonies were plated out and a mass plasmid DNA prep was done using Qiagen Plasmids containing the cDNA inserts were size selected on Sephacryl S-500 column in order to exclude plasmids with no insert. Early elutions were collected and transformed into DH5 alpha strain (Gibco/BRL).
      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      LP
      (Rubin et al., 2000)
      LP_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)