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General Information
Symbol
Dmel\CH322-115D4
Species
D. melanogaster
Name
CH322-115D4
FlyBase ID
FBcl0758132
Feature type
Computed gene(s)
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    CHORI-322_BAC
    • Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 21kb inserts.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      Sequence Data of the Insert
      Full Length Sequence
      Genomic Location
      3R:28,316,461..28,337,488 [+]
      Total bases
      21,028
      Full Length sequence dataSequence Downloader
      Left End Sequence
      Total bases
      701
      NCBI
      Left End sequence data
      GATCATTTTTTGGCCACGAAAGGCCAGTTTTATTCCGGGTTCCGGCCATTTCTGTTGCGGTGATAAAATTTTGACACAGG
      TTTGTCGACTCCTGCCACGCCCCCTCGATCCGGAATACGGATTCCGGAATTCACCAACCGGGCCGAACTAATTAGACGGC
      GCACCAAAGCGAAAATTTCGGTATTAAAATTGTTGAAAGCTCAAAATTATATTTATGGATCGCGGCAATAATTACGCTAA
      GCCAAAGTGCAAATTGTTATGCCTGTGGTTACCCACGTGCCCCCCACCCCTTTTGACCCATCACATGTCGAGTGTTTGTT
      CAGCCTGTGGAAAGTGGGCGTAATCTGAAACATGATGCACCATGTGGACAGGAATGGGTCTGGTTAGCGACAACCTATGA
      CAGGTTGTGTGGGTTTGTCCGGTGGCAGAACAGCAGGGATTAAAGGATTTAGTATATCTACAGTTTCTGTTAAAAAGGAG
      CAGCAGTCGCTGTGGATATTAAAGGAAACGCCAATAAATTGCAACATTGACGGCTATTCCAATCAGCCAAACTCCGAAAC
      ACACGACTTTTCCAGCCCACTTTACTCCGGAAGCATGTGCGTGAGCTAAGTGCGGACAAAGTTCAAAGGCGAGTCACATG
      TTAAACGCCCATTAAATTTAGCTTCGATATGAATGCGATATGTGAACCCGCCCCTGACCAA
      
      Right End Sequence
      Total bases
      738
      NCBI
      Right End sequence data
      GAACCTAATCTGCGAGGAGGGCGACAACGAGACCATGTCCTTCTCCACGCGTTCGCTGCAGATCCCGAGGACCGTGGACT
      GCCTGCAGGGCGTGCTCACGGTGATTCCGCTCCAGCTGCTGTCCTACCACATCGCCGTGCTCCGCGGCTGCGACGTGGAC
      TGCCCCAGGAACCTGGCCAAGTCGGTGACCGTGGAGTAGGCGCCCCAACTATCTGGCTAACTGGCTCGCACCGTCTCAAG
      AATTCCATTTTAGTATCTGCCTGAGAAAAACAAAACAAACCCTAGCACTTAAGTTAAAATTTTCTGGTATTTTGTCGGAT
      CAAAATCCGAACCCTTAACCCATTTGTGCTCCTTCTGGCTGAAGCCACATATGTTTAACCCCTACATTTGTAGCTGTACT
      GACCCTTTGTGTTGTGGACCCACTACTTTTTGTGAATGTGCAATTGAAATATAATTATTTCCAGTTTTTCTTTAAAAGTG
      TTTTGCTGCTTTCAATTAAAAATGGATTGGAAAATGTTTAACCAGTAAATTTGTTTGAAAACGGGAAAAACCAAACAAAG
      ATCTATATGTACCTCACTTTACAAAAAATGCTTATCTGACATTAAACATTTGTTTCAAATGTTTAAAAAACGAGGGCCAC
      GTCACGTTATTGCTTACGTTATGTTAATCACGTTGTAAATTTTAGAAAAGAAAATTACATATATATTTAATTCTGATAAA
      ACTAAATAAAAATAAATTC
      
      Library Information (1)
      Library: CHORI-322_BAC
      Description

      A BAC library containing genomic DNA fragments (average size of 21 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

      Sample preparation
      Protocol

      DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 20-25 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

      Comment

      A total of 73,728 clones were generated.

      Reported As
      Symbol Synonyms
      CHORI-322_BAC
      References (3)
      Research paper (2)
      Venken et al., 2010, Genetics 186(4): 1111--1125
      A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. [FBrf0212670]
      Venken et al., 2009, Nat. Methods 6(6): 431--434
      Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. [FBrf0209231]
      Personal communication to FlyBase (1)
      GenetiVision Corporation, 2016-, A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome.
      A collection of transgenic flies carrying duplications that tile the autosomal portion of the Drosophila melanogaster genome. [FBrf0232631]
      External Crossreferences and Linkouts ( 2 )
      Crossreferences
      NCBI Clone DB - A database that integrates information about clones and libraries, including sequence data, map positions and distributor information
      PACMANFLY - The source for information and resources of tools for the Drosophila community using the P[acman] recombineering technique.
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      CH322-115D4
      Secondary FlyBase IDs
        References (1)