5SRNA, 5S rRNA, 5S, l(2)03068, 5S RNA
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\5SrRNA using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\5SrRNA in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: 5SRNA l(2)03068
Extrachromosomal circular DNA (eccDNA) is present throughout the fly's life cycle. The eccDNA population contains circular multimers of tandemly repeated genes, including 5SrRNA.
Point mutations introduced in vitro into pre-5SrRNA have been used to determine which regions of the pre-RNA are required for processing.
The effects of stem I and loop A transversions, transitions, selected additions and deletions on 5SrRNA processing are studied.
The distribution of split 5.8S rRNA was studied in Diptera and Siphonaptera to learn the origin of 5.8S rRNA. Four species of mosquitoes and a flea have a single 5.8S rRNA. A crane fly, a midge, a robber fly, a house fly and D.melanogaster have split 5.8S rRNA.
In vitro transcription of isolated repeat units of 5SrRNA in a KcO cell extract reveals three 5SrRNA variants. The "5SI" variant is transcribed with a relatively high efficiency in vitro. The "5SII" variant is identical to "5SI", except for a two-nucleotide deletion at positions 28 and 29, and is transcribed in vitro at an efficiency of approximately 40% compared to the "5SI" variant. The "5SIII" variant has the same sequence as "5SI" except for a single G to A transition at position 86, but is not transcribed in vitro.
The Oregon R Yale stock contains various arrangements of the 5SrRNA cluster, one variant is due to the insertion of a roo element. Others, that lack the roo insertion, have a variety of deletions removing 0--60% of the genes at the insertion site. The Oregon R Heidelberg stock studied has no roo element so has no heterogeneity in cluster arrangement. D.melanogaster shows a large redundancy of 5SrRNA genes as there is no visible phenotype when homozygous or heterozygous against a total deletion of the cluster.
The locus encoding the genes for 5S RNA. Ordinarily, the haploid complement carries approximately 165 copies of 5S genes.
Heterozygous deficiency for the region is normal in phenotype.