A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Gene Dmel\Abl

General Information
SymbolDmel\AblSpeciesD. melanogaster
NameAbl tyrosine kinaseAnnotation symbolCG4032
Feature typeprotein_coding_geneFlyBase IDFBgn0000017
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map73B1-73B4Sequence location3L:16,615,461..16,641,674 [-]
Map ( GBrowse ) detailed view
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Automatically generated summary

See sections below for more information
The gene Abl tyrosine kinase is referred to in FlyBase by the symbol Abl (CG4032, FBgn0000017). It has the cytological map location 73B1-73B4. Its sequence location is 3L:16615461..16641674. Its molecular function is described as: protein-tyrosine kinase activity; protein binding; ATP binding; non-membrane spanning protein tyrosine kinase activity. It is involved in the biological processes described with 11 unique terms, many of which group under: anatomical structure development; cellular macromolecule metabolic process; biopolymer modification; protein modification process; regulation of cellular component organization and biogenesis; embryonic morphogenesis; regulation of biological process; regulation of protein stability; phosphorus metabolic process; ventral furrow formation; embryonic development via the syncytial blastoderm; central nervous system development; regulation of protein localization; cell motility; regulation of metabolic process; regulation of cell morphogenesis. 39 alleles are reported. The phenotypes of these alleles are annotated with 20 unique terms, many of which group under: nervous system; organ system; peripheral nervous system; embryonic nervous system; commissure; antennal segment; extended germ band embryo; adult segment; integumentary system; ventral furrow; embryonic neuron; late extended germ band embryo. It has 2 annotated transcripts and 2 annotated polypeptides.

External Summaries
hide Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage
Abl: Cellular abl oncogene sequence
Considered to be the Drosophila sequence homologous to mammalian c-abl based both on its origin and amino acid sequence as inferred from its nucleotide sequence. ABL protein detected at the time of germ-band shortening in the axons of the central nervous system in a bilateral symmetrical series of points that correspond to the positions of neuromeres; later, protein appears in the axons growing across the midline, but not in the cell bodies of the CNS nor in the PNS. As development proceeds, staining of the longitudinal fascicles and to a lesser extent the commissural fascicles becomes intense; staining also seen in association with axonal outgrowth of neural cells in the eye imaginal disk (Bennett and Hoffmann). Recessive alleles in combination with Df(3L)st-j7 = Df(3L)73A1-2;73B1-2 either die as late pupae or pharate adults with complete cuticle and roughened eyes, Abll1, or as short lived (5-6 days), rough-eyed adults, Abll2 and Abll3. Surviving females lay few eggs, some of which develop into adults; surviving males have motile sperm, but do not mate and produce no progeny. The rough eye is a reflection of some loss of photoreceptor cells plus ommatidial fusion. In combination with a deficiency extending further to the left, e.g., Df(3L)std11 = Df(3L)73A11-B1;73D1-2 to include the locus of Dab, Abll/Abl- genotypes die as late embryos or early first-instar larvae with disrupted axonal organization in the ventral nerve cord (Henkemeyer, Gertler, Goodman, and Hoffmann, Cell 51: 821-28). CNS of doubly deficient embryos, i.e., Abl- Dab-, fails to form commissures and is defective in axonal outgrowth, although the PNS develops normally.
hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
73B1-73B4  
Limits computationally determined from genome sequence between P{lacW}l(3)j10E8j10E8&P{PZ}l(3)1053210532 and P{PZ}Baldspot02281  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
73B1-73B2
(determined by in situ hybridisation)  
73B1-73B2
(determined by in situ hybridisation)  
73B-73B
(determined by in situ hybridisation)  
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
Gene Order (overall orientation not stated)
References
Overall orientation not stated: anon-73B+ Abl+
hide Gene Model & Products
Please see the GBrowse view of Dmel\Abl for information on other features
detailed view FBtr0075357 FBtr0112790 FBtr0075356 FBtr0075355 FBpp0075116 FBpp0111702 FBpp0075115 FBpp0075114 FBti0057856 FBti0045254 FBti0065787 FBti0011666 FBti0028842 FBti0050198 FBti0067584 FBti0067583 FBti0048358 FBti0009888 FBti0008016 FBti0055497 FBti0072570
Comments on Gene Model
DGC clone appears problematic: [DGC: ] LD03455 contains a frameshift (-C) at position 16621599 leading to shortened ORF relative to annotation.
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
FBtr0075357
  6707
  1620
FBtr0112790
  6761
  1638
Additional Transcript Data & Comments
Reported size (kB)
6.0, 5.5-6.0 (northern blot)
Comments
Abl transcripts are stated as being 5 and 3.3 kb, although a previous report (FBrf0041478) cited by the authors discusses only a 6.2 kb transcript.
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Abl-PA  
FBpp0075116  
171.6  
1620  
7.83  
Abl-PB  
FBpp0111702  
173.6  
1638  
8.10  
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
Studies of mutated Abl proteins reveal that sequences in the carboxy terminus mediate axonal localization. Disruption of proper subcellular localization correlates with lack of Abl biological function. A novel kinase-independent function of the Abl protein has been identified.
ena protein is a substrate for Abl tyrosine kinase.
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data
    Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     prepupal stage-pupal stage
     oogenesis stage,adult stage | female
     embryonic stage
     adult stage | female
     oogenesis stage,adult stage
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage
       embryonic central nervous system
       embryonic stage | late
       epithelial cell
       embryonic stage
       adepithelial cell
       embryonic stage | early
       epithelial cell | apical
       embryonic stage
       embryonic stage
       imaginal disc
       embryonic stage
       embryonic central nervous system
      Marker for
        Subcellular Localization
        CV Term
        hide Microarray Data
        Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)
        Untitled Document detailed view FBtr0075357 FBtr0112790 FBtr0075356 FBtr0075355
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        Other Phenotypes
        Allele
        Sterility
        Allele
        Phenotype manifest in
        Allele
        follicle cell & actin filament | somatic clone
        spindle & cleavage stage embryo | maternal effect
        actin cytoskeleton & embryo | maternal effect
        ventral adult lateral neuron & axon, with Scer\GAL4P2.4.Pdf
        hide Classical Alleles ( 19 )
        For All Classical Alleles Show

        Allele of AblClassMutagenStocksKnown lesion
        Abl046741 --
        Abl1hypomorph
          4 Yes
          Abl2amorph
            1 --
            Abl3hypomorph
              0 --
              Abl4hypomorph
                3 --
                AblDG03106
                  1 --
                  AblEP31011 Yes
                  AblEP35661 --
                  AblEY135321 --
                  Ablc051301 --
                  Ablc052130 --
                  Abld043201 --
                  Able035441 --
                  Ablf017781 --
                  Ablf033780 --
                  Abll10 --
                  Abll20 --
                  Abll30 --
                  Ablunspecified
                    0 --
                    hide Alleles Carried on Transgenic Constructs ( 20 )
                    For All Alleles Carried on Transgenic Constructs Show

                    Allele of AblClassMutagenStocksKnown lesion
                    Abl+mTnabl-ala0 Yes
                    Abl+mTnabl-lys0 Yes
                    Abl+mTnabl-ts0 Yes
                    Abl+mTnablhypomorph0 Yes
                    Abl+tEa0 --
                    Abl2kb.T:Avic\GFP-EGFP0 Yes
                    AblAct5C.PC0 Yes
                    AblAct5C.PG0 Yes
                    AblAct5C.PW0 Yes
                    AblGD13441 Yes
                    AblK417N.Act5C0 Yes
                    AblK417N.Scer\UAS1 Yes
                    AblMtnA.T:Hsap\MYC0 Yes
                    AblScer\UAS.T:Avic\GFP-EGFP0 Yes
                    AblScer\UAS.cFa2 Yes
                    AblW118A.Act5C0 Yes
                    AbldsRNA.cFa0 Yes
                    AbldsRNA.cLa0 Yes
                    AbldsRNA.cRa0 Yes
                    AbldsRNA.cWa0 Yes
                    hide Aneuploid Aberrations
                    Useful deficiency
                    Useful duplication
                    Disrupted in