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Gene Dmel\acj6

General Information
Symbol	Dmel\acj6	Species	D. melanogaster
Name	abnormal chemosensory jump 6	Annotation symbol	CG9151
Feature type	protein_coding_gene	FlyBase ID	FBgn0000028
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	I-POU
Genomic Location
Chromosome (arm)	X	Recombination map	1-49.4
Cytogenetic map	13B8-13C1	Sequence location	X:15,245,315..15,272,487 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene abnormal chemosensory jump 6 is referred to in FlyBase by the symbol Dmel\acj6 (CG9151, FBgn0000028). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sequence-specific DNA binding transcription factor activity; transcription corepressor activity. There is experimental evidence that it is involved in the biological process: synaptic target recognition; chemosensory jump behavior; positive regulation of transcription from RNA polymerase II promoter; axon guidance; detection of chemical stimulus involved in sensory perception of smell; dendrite morphogenesis; dendrite guidance. 21 alleles are reported. The phenotypes of these alleles are annotated with: adult olfactory receptor neuron Or42a; antennal sense organ; adult olfactory receptor neuron Or71a; lamina monopolar neuron L3; lamina plexus; maxillary palp sensillum basiconicum; lamina monopolar neuron L1; maxillary palp sense organ. It has 13 annotated transcripts and 13 annotated polypeptides. Protein features are: Homeobox, conserved site; Homeodomain; Homeodomain-like; Lambda repressor-like, DNA-binding domain; POU domain; POU-specific. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1631102_a_at completely aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of acj6. Gene sequence location is X:15245315..15272487.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms A1-7 LL1 motor neuron A1-7 VO2 motor neuron A1-7 DO2 motor neuron
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13B8-13C1   Limits computationally determined from genome sequence between P{EP}hiwEP1308 and P{EP}Ahcy13EP1007
Experimentally Determined Cytological Location
Cytogenetic map Notes References 13C1-13C1     (Clyne, 1998.8.25)
Experimentally Determined Recombination Data
Location	1-49.4 (Carlson, 1993)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\acj6 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) acj6-RA FBtr0074015  NM_080137   2080   396 acj6-RC FBtr0100326  NM_001014744   1996   368 acj6-RD FBtr0100328  NM_001014743   2774   394 acj6-RE FBtr0290063  NM_167438   2017   375 acj6-RF FBtr0290064  NM_001144726   1984   364 acj6-RG FBtr0305189  NM_001258753   2053   387 acj6-RH FBtr0305190  NM_001258754   1963   357 acj6-RI FBtr0305191  NM_001258755   2690   366 acj6-RJ FBtr0305192  NM_001258756   1969   359 acj6-RK FBtr0305193  NM_001258757   2002   370 acj6-RL FBtr0305194  NM_001258758   2020   376 acj6-RM FBtr0305195  NM_001258759   2041   383 acj6-RN FBtr0305196  NM_001258760   2026   378
Additional Transcript Data & Comments
Reported size (kB)	1.7 (northern blot) (Treacy et al., 1991)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein acj6-PA   FBpp0073832   43.7   396   9.54     NP_524876   AAF48447 acj6-PC   FBpp0099732   40.4   368   9.36     NP_001014744   AAX52497 acj6-PD   FBpp0099734   43.4   394   9.19     NP_001014743   AAX52498 acj6-PE   FBpp0288502   41.3   375   9.36     NP_727845   AAN09663 acj6-PF   FBpp0288503   40.1   364   9.81     NP_001138198   ACL82930 acj6-PG   FBpp0293719   42.6   387   9.19     NP_001245682   AFH07396 acj6-PH   FBpp0293720   39.2   357   9.81     NP_001245683   AFH07397 acj6-PI   FBpp0293721   40.4   366   10.01     NP_001245684   AFH07398 acj6-PJ   FBpp0293722   39.5   359   10.01     NP_001245685   AFH07399 acj6-PK   FBpp0293723   40.7   370   9.67     NP_001245686   AFH07400 acj6-PL   FBpp0293724   41.4   376   9.68     NP_001245687   AFH07401 acj6-PM   FBpp0293725   42.2   383   9.68     NP_001245688   AFH07402 acj6-PN   FBpp0293726   41.7   378   9.92     NP_001245689   AFH07403
Additional Polypeptide Data & Comments
Reported size (kDa)	369 (aa) (Treacy et al., 1992) 367 (aa); 44 (kD) (Treacy et al., 1991)
Comments	A partial peptide which results from alternative splicing and gives rise to a homeodomain that is two amino acids longer than its alternative Ipou protein. (FlyBase, 1992-) A splice variant of the acj6 gene (previously called Twin of I-POU), has 2 additional basic residues (RK) with respect to other isoforms which allows it to specifically bind DNA. This form of the acj6 protein does not interact with vvl protein but can act as a positive transcription factor on targets distinct from those regulated by vvl protein. Two different isoforms of acj6 protein appear to exert complementary functions, simultaneously activating and inhibiting two distinct sets of transcription units. (Treacy et al., 1992) acj6 protein cannot bind DNA due to the lack of two basic amino acids in the N-terminal region of its POU homeodomain that are present in other POU doman genes. Immunoprecipitation and crosslinking studies show that acj6 protein associates with vvl protein in vivo. When bound by acj6 protein as a heterodimer, vvl protein no longer binds the enhancer site of the Ddc gene. Transfection experiments in CV-1 cells demonstrate that vvl protein activates transcription of a reporter gene driven by the Ddc promoter or a heterologous promoter preceded by the vvl element. This activation does not occur in the presence of acj6 protein. (Treacy et al., 1991)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • POU domain (IPR013847) Homeobox, conserved site (IPR017970) POU-specific (IPR000327) Homeodomain (IPR001356) Homeodomain-like (IPR009057) Lambda repressor-like, DNA-binding domain (IPR010982)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014298 AAF48447   AE014298 AAN09663   AE014298 AAX52497   AE014298 AAX52498   AE014298 ACL82930   AF086816 AAC35369   AE014298 AFH07396   AE014298 AFH07397   AE014298 AFH07398   AE014298 AFH07399   AE014298 AFH07400   AE014298 AFH07401   AE014298 AFH07402   AE014298 AFH07403   BF502015   BI627120   BT028815   BT028848   CO327308   CO327740   CO341205   CO342904   CZ490303   CZ490304   EC073113   EC074795   EC222009   EC235975   EC245013   EC254147   EC258253   EC258784   GH909645   S82267   S82271 AAB21441   X58436 CAA41342
UniProtKB/Swiss-Prot	P24350
UniProtKB/TrEMBL	B7Z0Y6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
No Assay Recorded Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval central nervous system   (Treacy et al., 1991) EL neuron   (Treacy et al., 1991) ventral nerve cord   (Treacy et al., 1991) pCC neuron   (Treacy et al., 1991) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   RP1 motor neuron   (Treacy et al., 1991) U neuron   (Treacy et al., 1991) aCC neuron   (Treacy et al., 1991) supraesophageal ganglion   (Treacy et al., 1991) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) longitudinal visceral muscle primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic central brain neuron   (Fisher et al., 2012) embryonic head sensory system   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13 -- 14   embryonic brain   (Certel et al., 2000) ventral nerve cord | restricted   (Certel et al., 2000) antenno-maxillary complex | precursor   (Certel et al., 2000) third instar larval stage   interneuron of ventral nerve cord   (Certel et al., 2000) Comment:reference states "SP interneurons" embryonic/larval brain | restricted   (Certel et al., 2000) adult stage   adult antennal lobe projection neuron DL1 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron D adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA3 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA1d adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VM7 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VM2 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron DM6 adPN   (Komiyama and Luo, 2007) adult antennal lobe projection neuron VA1v adPN   (Komiyama and Luo, 2007) optic lobe   (Certel et al., 2000) adult central complex   (Certel et al., 2000) antennal sense organ | subset   (Jafari et al., 2012) adult olfactory receptor neuron Or92a | subset   (Jafari et al., 2012) adult olfactory receptor neuron Or47b | subset   (Jafari et al., 2012)
Additional Descriptive Data
	acj6 protein is expressed in a subset of olfactory sense organs in the antenna. These include some sensilla labelled by Mmus\Cd8aOr47b.330.T:Avic\GFP or Mmus\Cd8aOr92a.Avic\GFP.T:Hsap\MYC. (Jafari et al., 2012) acj6 protein is expressed in postmitotic neurons, but never in embryonic neuroblasts or ganglion mother cells. (Certel et al., 2000)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    27.25 Larval Midgut    1.9 Larval Hindgut    1.6 Larval Malpighian Tubules    2 Larval Fat Body    15.1 Larval Salivary Gland    4.7 Larval Trachea    1.475 Larval Carcass    2.425 Adult Head    9.1 Adult Eye    4.725 Adult Brain    84.8 Adult Thoracic-Abdominal Ganglion    21.6 Adult Crop    2.7 Adult Midgut    3.9 Adult Hindgut    1.9 Adult Malpighian Tubules    3.4 Adult Fat Body    6.1 Adult Salivary Gland    5.6 Adult Heart    2.375 Adult VirginFemale Spermatheca    4.4 Adult InseminatedFemale Spermatheca    5.2 Adult Ovary    0.4 Adult Testis    59.9 Adult Male Accessory Gland    8.8 Adult Carcass    3.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    9.1   NA  Eye    4.725   NA  Brain    84.8   27.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    21.6   NA  Crop    2.7   1.9  Midgut    3.9   1.6  Hindgut    1.9   2  Malpighian Tubules    3.4   no informative data  Fat Body    6.1   4.7  Salivary Gland    5.6   NA  Heart    2.375   1.475  Trachea    NA   NA  VirginFemale Spermatheca    4.4   NA  InseminatedFemale Spermatheca    5.2   NA  Ovary    0.4   NA  Testis    59.9   NA  Male Accessory Gland    8.8   2.425  Carcass    3.3 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    35 central nervous system, pupae P8    41 head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female    28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3  (35) central nervous system, pupae P8  (41) head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female  (28) head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    35 central nervous system, pupae P8    41 head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female    28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    35 central nervous system, pupae P8    41 head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female    28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    35 central nervous system, pupae P8    41 head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female    28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3  (35) central nervous system, pupae P8  (41) head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female  28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    35 central nervous system, pupae P8    41 head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female    28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    35 central nervous system, pupae P8    41 head, virgin 1-day female    19 head, virgin 4-day female    22 head, virgin 20-day female    7 head, mated 1-day female    28 head, mated 4-day female    16 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    25 head, mated 20-day male    17 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    3 fat body, pupae P8    15 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    17 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    1 embryo 12-14hr    2 embryo 14-16hr    2 embryo 16-18hr    2 embryo 18-20hr    2 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    1 larva L2    1 larva L3 12hr old    0 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    1 larva L3 puffstage 7-9    1 white prepupae new    1 white prepupae 12hr    2 white prepupae 24hr    3 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    2 adult male 05day    4 adult male 30day    5 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    0 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    0 embryonic Kc167    0 embryonic S1    22 embryonic S3    0 leg disc CME L1    0 wing disc CME-W2    3 wing disc ML-DmD8    0 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    0 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    0 tumorous blood cells mbn2    0 ovary fGS/OSS    1 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    7 heat shock, 4-day adult    1 Cadmium 50 mM 6 hrs, larvae L3    0 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    3 Cadmium 100 mM 48 hrs, 4-day adult    5 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_24 mE1_20_mRNA_expression_cluster_07
External Data & Images
Linkouts	BDGP expression data • RT01016 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000028 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9151-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage, with Scer\GAL4Cha.7.4 acj61,4.Scer\UAS lethal | pupal stage, with Scer\GAL4Cha.7.4 acj61,4.Scer\UAS lethal | recessive acj6PG63 viable acj62 acj63 acj65 acj66 acj6f07555 viable, with Scer\GAL4pnr-MD237 acj6GD4681 Sterility Allele fertile acj6f07555 Other Phenotypes Allele behavior defective | larval stage acj61 behavior defective | recessive acj61 acj62 acj63 acj65 acj66 chemical sensitive acj61 chemosensitive behavior defective | recessive acj61 acj6n locomotor behavior defective | adult stage (with acj61) acj66 locomotor behavior defective | adult stage (with acj66) acj61 locomotor behavior defective | adult stage | recessive acj61 acj66 locomotor behavior defective | larval stage | recessive acj61 acj66 neuroanatomy defective acj66 neuroanatomy defective, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS neuroanatomy defective | cell autonomous | male acj66 neuroanatomy defective | cell autonomous | somatic clone acj66 neuroanatomy defective | cell non-autonomous | male acj66 neuroanatomy defective | cell non-autonomous | somatic clone acj66 neuroanatomy defective | recessive acj66 neuroanatomy defective | somatic clone acj66 neurophysiology defective, with Scer\GAL4GH146 acj61,4.Scer\UAS neurophysiology defective | recessive acj61 acj62 acj63 acj65 acj66 neurophysiology defective | somatic clone acj66 smell perception defective acj61 smell perception defective | recessive acj61 acj62 acj63 acj65 acj66 Phenotype manifest in Allele abdominal ventral longitudinal muscle 1 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 2 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS abdominal ventral longitudinal muscle 4 & neuromuscular junction, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS adult antennal lobe projection neuron D adPN | somatic clone acj66 adult antennal lobe projection neuron DL1 adPN | somatic clone acj66 adult antennal lobe projection neuron DM6 adPN | somatic clone acj66 adult antennal lobe projection neuron VA1v adPN | somatic clone acj66 adult antennal lobe projection neuron VA3 adPN | somatic clone acj66 adult antennal lobe projection neuron VM2 adPN | somatic clone acj66 adult olfactory receptor neuron Or42a acj66 acj6PG63 adult olfactory receptor neuron Or71a acj66 acj6PG63 antennal glomerulus & neuron, with Scer\GAL4GH146 acj61,4.Scer\UAS antennal glomerulus & neuron | somatic clone acj66 antennal glomerulus D & neuron | somatic clone acj66 antennal glomerulus DL1 & neuron | somatic clone acj66 antennal glomerulus DM6 & neuron | somatic clone acj66 antennal glomerulus VA1 & neuron | somatic clone acj66 antennal glomerulus VA3 & neuron | somatic clone acj66 antennal glomerulus VM2 & neuron | somatic clone acj66 antennal lobe glomerulus | male acj66 antennal lobe glomerulus D | somatic clone acj66 antennal lobe glomerulus DL1 | somatic clone acj66 antennal lobe glomerulus DM6 | somatic clone acj66 antennal lobe glomerulus VA1v | somatic clone acj66 antennal lobe glomerulus VA3 | somatic clone acj66 antennal lobe glomerulus VM2 | somatic clone acj66 antennal sense organ acj61 anterior fascicle & motor neuron, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS axon & olfactory neuron & antennal glomerulus DA4 | male | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus DA4 | somatic clone | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus DM3 | male | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus DM3 | somatic clone | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus V | male | cell non-autonomous acj66 axon & olfactory neuron & antennal glomerulus V | somatic clone | cell non-autonomous acj66 axon & olfactory neuron & antennal glomerulus VA6 | male | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus VA6 | somatic clone | cell autonomous acj66 axon & olfactory neuron & antennal glomerulus VC2 | male | cell non-autonomous acj66 axon & olfactory neuron & antennal glomerulus VC2 | somatic clone | cell non-autonomous acj66 axon & olfactory neuron | male | ectopic acj66 axon & olfactory neuron | somatic clone | ectopic acj66 bouton | ectopic, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS lamina monopolar neuron L1 acj66 lamina monopolar neuron L3 acj66 lamina plexus acj66 lamina plexus (with acj61) acj66 lamina plexus (with acj66) acj61 maxillary palp sense organ acj61 maxillary palp sensillum basiconicum acj66 monopolar laminar cell L1 & synapse acj66 monopolar laminar cell L3 & synapse acj66 motor neuron | embryonic stage, with Scer\GAL4sca-537.4 acj61,4.Scer\UAS neuromuscular junction | ectopic, with Scer\GAL4sca-537.4 acj61,3,4.Scer\UAS olfactory neuron & maxillary palp sense organ acj66 olfactory sensory organ & neuron & dendrite, with Scer\GAL4GH146 acj61,4.Scer\UAS olfactory sensory organ & neuron & dendrite | somatic clone acj66
Classical Alleles ( 11 )
For All Classical Alleles Show
Allele of acj6 Class Mutagen Stocks Known lesion acj6f07555 piggyBac transposase 2 -- acj6PG63 Delta2-3 2 -- acj6MB06436 1 -- acj6NP3476 1 -- acj6n amorphic allele - genetic evidence 0 -- acj61 loss of function allele, hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes acj62 loss of function allele X ray 0 Yes acj63 X ray 0 Yes acj65 X ray 0 Yes acj66 loss of function allele ethyl methanesulfonate 0 Yes acj6unspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of acj6 Class Mutagen Stocks Known lesion acj6GD17029 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes acj6GD4681 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes acj6JF02497 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes acj6KK108876 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes acj6T:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP in vitro construct - coding region fusion 1 Yes acj61,3,4.Scer\UAS in vitro construct - regulatory fusion 0 Yes acj61,4.Scer\UAS in vitro construct - regulatory fusion 0 Yes acj6I.Scer\UAS in vitro construct - regulatory fusion 0 Yes acj6J.Scer\UAS in vitro construct - regulatory fusion 0 Yes acj6VDRC.cUa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)M34-1Cs (Clyne et al., 1999)
Duplicated in	Dp(1;2)eagx6 (Clyne et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4681} NA P{GD17029} NA P{KK108876} NA P{TRiP.JF02497} NA P{UAS-acj6.1,3,4} NA P{UAS-acj6.1,4} NA P{UAS-acj6.isoI} NA P{UAS-acj6.isoJ} NA characterization construct P{VDRC.acj6.U} NA PBac{acj6-GFP.FPTB} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}acj6f07555 NA insertion of enhancer trap binary system Mi{ET1}acj6MB06436 No P{GawB}acj6NP3476 No P{GawB}acj6PG63 No
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity inferred from direct assay (Lee and Salvaterra, 2002) transcription corepressor activity inferred from genetic interaction with vvl (Treacy et al., 1991) inferred from physical interaction with vvl (Treacy et al., 1991)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Komiyama et al., 2004) chemosensory jump behavior inferred from mutant phenotype (McKenna et al., 1989) dendrite guidance inferred from mutant phenotype (Komiyama and Luo, 2007) dendrite morphogenesis inferred from mutant phenotype (Komiyama et al., 2003) detection of chemical stimulus involved in sensory perception of smell inferred from direct assay (Bai and Carlson, 2010) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Lee and Salvaterra, 2002) synaptic target recognition inferred from mutant phenotype (Certel et al., 2000)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
protein serine/threonine phosphatase activity inferred from sequence or structural similarity with EMBL:U53276 (FlyBase, 1992-) sequence-specific DNA binding inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR017970 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
dendrite morphogenesis traceable author statement (Grueber and Jan, 2004)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with worm unc-86 (Treacy et al., 1991) non-traceable author statement (Swiss-Prot Project Members, 1992.3.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions acj6 allele Gene References acj66 vvl (Komiyama et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 70572 DroID - A comprehensive database of gene and protein interactions. • FBgn0000028 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47080
Orthologs
OrthoDB Orthologs (48) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HHPNC Dipteran inclusive ortholog search EOG6DZ23X Insect inclusive ortholog search EOG66T1XH Arthropod inclusive ortholog search EOG6V6WZK Metazoa inclusive ortholog search EOG6R874Z
Orthologs in Drosophila Species (EOG6HHPNC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\acj6     Drosophila simulans   Dsim\GD15807     Drosophila sechellia   Dsec\GM25017     Drosophila erecta   Dere\GG19406 Y   Drosophila yakuba   Dyak\GE16054     Drosophila ananassae   Dana\GF21043     Drosophila pseudoobscura pseudoobscura   Dpse\GA26624     Drosophila persimilis   Dper\GL14745     Drosophila willistoni   Dwil\GK20016     Drosophila virilis   Dvir\GJ16375 Y   Drosophila mojavensis   Dmoj\GI15417     Drosophila grimshawi   Dgri\GH17948
Orthologs in non-Drosophila Dipterans (EOG6DZ23X)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005507   Anopheles gambiae Malaria mosquito  Agam\AGAP002172   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ014571
Orthologs in non-Dipteran Insects (EOG66T1XH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45925   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006566   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12824   Atta cephalotes Leafcutter ant  Acep\ACEP22036   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20967   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15432   Linepithema humile Argentine ant  Lhum\LH18452   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16360   Solenopsis invicta Red fire ant  Sinv\SINV13061   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002057   Bombyx mori Silkmoth  Bmor\BGIBMGA002017   Pediculus humanus Human body louse  Phum\PHUM425120   Tribolium castaneum Red flour beetle  Tcas\acj6
Orthologs in non-Insect Arthropods (EOG6V6WZK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Senp7   Ixodes scapularis Deer tick  Isca\ISCW007997
Orthologs in non-Arthropod Metazoa (EOG6R874Z)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\C30A5.7   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025632gn   Danio rerio Zebrafish  Drer\zgc:158291   Danio rerio Zebrafish  Drer\pou4f1   Danio rerio Zebrafish  Drer\pou4f2   Danio rerio Zebrafish  Drer\pou4f3   Xenopus tropicalis Western clawed frog  Xtro\pou4f1   Xenopus tropicalis Western clawed frog  Xtro\pou4f2   Xenopus tropicalis Western clawed frog  Xtro\pou4f3   Xenopus tropicalis Western clawed frog  Xtro\pou4f1.2   Gallus gallus Domestic chicken  Ggal\LOC428735   Gallus gallus Domestic chicken  Ggal\ENSGALG00000014195   Mus musculus House mouse  Mmus\Pou4f3   Mus musculus House mouse  Mmus\Pou4f2   Rattus norvegicus Norway rat  Rnor\Pou4f2   Rattus norvegicus Norway rat  Rnor\Pou4f3   Homo sapiens Human  Hsap\POU4F2   Homo sapiens Human  Hsap\POU4F3
Human Orthologs (2)
Gene OMIM HGNC Hsap\POU4F2     Hsap\POU4F3
AAA Orthologs (2) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila erecta Dere\GG19406 Drosophila virilis Dvir\GJ16375
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	19079 w1118 PBac{WH}acj6f07555 26047 w1118 Mi{ET1}acj6MB06436 42262 w1118; PBac{acj6-GFP.FPTB}VK00033 29335 y1 v1; P{TRiP.JF02497}attP2 30025 y1 w1118 P{GawB}acj6PG63/FM7i 30028 y1 w* P{UAS-mCD8::GFP.L}Ptp4ELL4 P{GawB}acj6PG63; Pin1/CyO
Harvard	- PBac{WH}acj6f07555
Kyoto	104519 y* w* P{GawB}acj6NP3476 / FM7c
VDRC	v105292 P{KK108876}VIE-260B v9827 w1118; P{GD4681}v9827 v47881 w1118; P{GD17029}v47881/TM3
Genomic Clones ( 2 )
	BACR36D15 BACR48D17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 16 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	RT01016 RT01116
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT17624 RH68334
Other clones	37287 37351 66112 77394 214413.30 214413.31 214449.31 EP06745 EP07945 EP12036 EP15720 RP003159313
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000028 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47080
Antibody Information
	polyclonal (Treacy et al., 1991)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: acj6 CG9151 (Bayraktaroglu, 2000.4.13)
Additional comments
Other Comments
	acj6 may regulate dendritic targeting and coordinate dendritic and axonal connectivity of projection neurons in the olfactory system to ensure the highly stereotypes acquisition and delivery of olfactory information by the central olfactory neurons. (Komiyama et al., 2003) acj6 may play an important role in regulating synaptic target selection by central neurons. (Certel et al., 2000) Loss of acj6 function in the larval optic lobe neurons results in disorganised retinal axon targeting and synapse selection. (Certel et al., 2000) acj6 mutations disrupt the Ipou gene. (Clyne, 1997.4.19) acj6 protein does not affect DNA binding by the vvl protein, and no interaction between the acj6 and vvl proteins can be detected. (Turner, 1996) An alternative transcript of Ipou is incapable of dimerizing with Cf1a but can act as a positive transcription factor on targets distinct from those regulated by Cf1a. This suggests that the Ipou locus simultaneously generates both a specific activator and inhibitor of gene transcription. (Treacy et al., 1992) Using the electroantennogram (EAG) to measure antennal physiology, an adult antennal defect in the olfactory behaviour was found in acj61 flies. (Ayer and Carlson, 1991) Ipou gene product cannot bind DNA as it lacks two basic amino acids in the N-terminal region of its POU homeodomain that are present in other POU doman genes. Ipou is coexpressed in subsets of neurons with Cf1a during development. Ipou forms a highly stable heterodimeric complex with Cf1a and inhibits its ability to bind DNA and activate transcription of Ddc. (Treacy et al., 1991) Mutants have an abnormal jump response. (McKenna et al., 1989) Mutants have an abnormal jump response and an abnormal electroantennagram.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080137 NM_167438 NM_001014743 NM_001014744 NM_001144726 NM_001258753 NM_001258754 NM_001258755 NM_001258756 NM_001258757 NM_001258758 NM_001258759 NM_001258760 RefSeq (Proteins) • NP_524876 NP_727845 NP_001014743 NP_001014744 NP_001138198 NP_001245682 NP_001245683 NP_001245684 NP_001245685 NP_001245686 NP_001245687 NP_001245688 NP_001245689 DDBJ / EMBL / GenBank • AAB21441 AAC35369 AAF48447 AAF48447.2 AAN09663 AAN09663.1 AAX52497 AAX52498 ACL82930 AF086816 AFH07396 AFH07397 AFH07398 AFH07399 AFH07400 AFH07401 AFH07402 AFH07403 CAA41342 CZ490303 CZ490304 S82267 S82271 X58436 Entrez Gene - A searchable database of RefSeq genes. • 47080 UniProtKB/Swiss-Prot • P24350 UniProtKB/TrEMBL • B7Z0Y6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • POU domain (IPR013847) Homeobox, conserved site (IPR017970) POU-specific (IPR000327) Homeodomain (IPR001356) Homeodomain-like (IPR009057) Lambda repressor-like, DNA-binding domain (IPR010982) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RT01016
Linkouts
	BioGRID - A database of protein and genetic interactions • 70572 DroID - A comprehensive database of gene and protein interactions. • FBgn0000028 DRSC - Results from RNAi screens. • FBgn0000028 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000028 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9151-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000028 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47080 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/ipou1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47080 modMine - Data generated by the modENCODE project. • FBgn0000028
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	Acj6 (Jan and Yan, 2003, Certel and Johnson, 2000, Smith, 1999, Das et al., 2010, Alenius and Dickson, 2007) ACJ6 (Goldman et al., 2001) acj6 (Bai et al., 2007, de Bruyne and Warr, 2006, Komiyama et al., 2004, Goldman et al., 2005, Jafari et al., 2012, Gao and Bogert, 2003, Jefferis et al., 2007, Vosshall, 2007, Suster et al., 2003, Lichtneckert et al., 2008, Bashaw, 2007, Ray et al., 2008, Tichy et al., 2008, Erclik et al., 2008, Zimmermann et al., 2009, Hall, 2003, Komiyama and Luo, 2007, Glazov et al., 2005, Blagburn, 2008, Lin et al., 2010, Lai et al., 2008, Bai and Carlson, 2010, Bai et al., 2009, Busser et al., 2012, Spokony and White, 2012.11.14) CG9151 (Bourbon et al., 2002, Keleman et al., 2009.8.5, Perkins et al., 2009) I-POU (Treacy, 1992.5.29, Gates, 1999, Koizumi et al., 1997, Certel and Johnson, 1997, Clyne et al., 1997, Odenwald et al., 1997, Turner, 1996, Lopez, 1995, Gehring et al., 1994, ) Ipou   tI-POU (Rhee et al., 1998)
Name Synonym	Abnormal chemosensory jumb (Kuzin et al., 2005) abnormal chemosensory jump 6 (Goldman et al., 2005, Zimmermann et al., 2009, Glazov et al., 2005) Abnormal chemosensory jump 6 (Bai et al., 2009) altered chemosensory jump 6 (Blagburn, 2008) Twin of I-POU (Treacy, 1992.5.29)
Secondary FlyBase IDs
	FBgn0004418 FBgn0030633
References ( 113 )
Generate a list of	All references relating to this gene ( 113 )
List References by type	Research paper  ( 43 ) Supplementary material  ( 3 ) Review  ( 25 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 19 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Letter  ( 1 )
Recent research papers ( 3 )
	Busser et al., 2012, PLoS Genet. 8(3): e1002531 A machine learning approach for identifying novel cell type-specific transcriptional regulators of myogenesis. [FBrf0217716] Jafari et al., 2012, PLoS Biol. 10(3): e1001280 Combinatorial activation and repression by seven transcription factors specify Drosophila odorant receptor expression. [FBrf0217822] Prieto-Godino et al., 2012, PLoS Biol. 10(10): e1001400 Embryonic origin of olfactory circuitry in Drosophila: contact and activity-mediated interactions pattern connectivity in the antennal lobe. [FBrf0219665] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...