Gene model reviewed during 5.53
Gene model reviewed during 5.56
1.7 (northern blot)
None of the polypeptides share 100% sequence identity.
A partial peptide which results from alternative splicing and gives rise to a homeodomain that is two amino acids longer than its alternative Ipou protein.
A splice variant of the acj6 gene (previously called Twin of I-POU), has 2 additional basic residues (RK) with respect to other isoforms which allows it to specifically bind DNA. This form of the acj6 protein does not interact with vvl protein but can act as a positive transcription factor on targets distinct from those regulated by vvl protein. Two different isoforms of acj6 protein appear to exert complementary functions, simultaneously activating and inhibiting two distinct sets of transcription units.
acj6 protein cannot bind DNA due to the lack of two basic amino acids in the N-terminal region of its POU homeodomain that are present in other POU doman genes. Immunoprecipitation and crosslinking studies show that acj6 protein associates with vvl protein in vivo. When bound by acj6 protein as a heterodimer, vvl protein no longer binds the enhancer site of the Ddc gene. Transfection experiments in CV-1 cells demonstrate that vvl protein activates transcription of a reporter gene driven by the Ddc promoter or a heterologous promoter preceded by the vvl element. This activation does not occur in the presence of acj6 protein.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\acj6 using the Feature Mapper tool.
Comment: Authors call this '1 adPN'.
Comment: Authors call this 'poly[L1]'
Comment: Authors call this 'poly[L2]'
Comment: Authors call this 'poly[L3]'
Comment: Authors call this 'poly[L4]'
Comment: Authors call this 'poly[emb]'
GBrowse - Visual display of RNA-Seq signalsView Dmel\acj6 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: acj6 CG9151
acj6 may regulate dendritic targeting and coordinate dendritic and axonal connectivity of projection neurons in the olfactory system to ensure the highly stereotypes acquisition and delivery of olfactory information by the central olfactory neurons.
acj6 may play an important role in regulating synaptic target selection by central neurons.
Loss of acj6 function in the larval optic lobe neurons results in disorganised retinal axon targeting and synapse selection.
An alternative transcript of Ipou is incapable of dimerizing with Cf1a but can act as a positive transcription factor on targets distinct from those regulated by Cf1a. This suggests that the Ipou locus simultaneously generates both a specific activator and inhibitor of gene transcription.
Using the electroantennogram (EAG) to measure antennal physiology, an adult antennal defect in the olfactory behaviour was found in acj61 flies.
Ipou gene product cannot bind DNA as it lacks two basic amino acids in the N-terminal region of its POU homeodomain that are present in other POU doman genes. Ipou is coexpressed in subsets of neurons with Cf1a during development. Ipou forms a highly stable heterodimeric complex with Cf1a and inhibits its ability to bind DNA and activate transcription of Ddc.
Mutants have an abnormal jump response.
Mutants have an abnormal jump response and an abnormal electroantennagram.