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Gene Dmel\nAcRα-96Aa

General Information
Symbol	Dmel\nAcRα-96Aa	Species	D. melanogaster
Name	nicotinic Acetylcholine Receptor alpha 96Aa	Annotation symbol	CG5610
Feature type	protein_coding_gene	FlyBase ID	FBgn0000036
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	ALS, nAChR, Acr96Aa, dALS
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96A1-96A2	Sequence location	3R:20,226,948..20,282,651 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nicotinic Acetylcholine Receptor alpha 96Aa is referred to in FlyBase by the symbol Dmel\nAcRα-96Aa (CG5610, FBgn0000036). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: acetylcholine-activated cation-selective channel activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: ion transport. 10 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Neurotransmitter-gated ion-channel; Neurotransmitter-gated ion-channel ligand-binding; Neurotransmitter-gated ion-channel transmembrane domain; Neurotransmitter-gated ion-channel, conserved site; Nicotinic acetylcholine receptor. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed within 12-24 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1632060_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of nAcRalpha-96Aa. Gene sequence location is 3R:20226948..20282651.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Acr96A Structural gene encoding a Drosophila homologue of a subunit of vertebrate nicotinic acetylcholine receptors (nAChR); inferred to be homologous to neuronal α subunits based on the cystein doublet at amino-acid residues 201 and 202.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Alleles nAcRalpha-96Aa[MI03525] Insertions Mi{MIC}nAcRalpha-96Aa[MI03525] References Bellen, 2011.9.26, MI948.xml
FB2012_01	Sequence features HFA17008 HFA13672 BKN30755 HFA13670 HFA13664
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96A1-96A2
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nAcRα-96Aa for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nAcRα-96Aa-RA FBtr0084619  NM_079757   3469   567
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nAcRα-96Aa-PA   FBpp0084003   64.0   567   4.87     NP_524481   AAF56301
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments	nAcRα-96Aa protein appears to be the ligand-binding subunit of the class I α-Btx binding complex. (Schloss et al., 1991)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Neurotransmitter-gated ion-channel, conserved site (IPR018000) Nicotinic acetylcholine receptor (IPR002394) Neurotransmitter-gated ion-channel transmembrane domain (IPR006029) Neurotransmitter-gated ion-channel (IPR006201) Neurotransmitter-gated ion-channel ligand-binding (IPR006202)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695447   AE014297 AAF56301   BI613336   BI626642   CL706578   EY198624   EY198688   X07194 CAA30172
UniProtKB/Swiss-Prot	P09478
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference larval stage   fat body   (Colombani et al., 2003) embryonic/larval brain | restricted   (Colombani et al., 2003)
Additional Descriptive Data
	Transcript is detected in the larval fat body and in the clusters of cells in the pars intercerebralis corresponding to the medial neurosecretory cell clusters (m-NSCs) that express the insulin like protein genes. (Colombani et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0000036    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0000036 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed within 12-24 hour embryonic stages, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0000036 expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr  (257) embryo 16-18hr  (464) embryo 18-20hr  (576) embryo 20-22hr  (466) embryo 22-24hr  (380) larva L1  (593) larva L2  (120) larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr  (116) white prepupae 24hr  (168) pupae 2d postWPP  (288) pupae 3d postWPP  (230) pupae 4d postWPP  (111) adult male 01day  (153) adult male 05day  (221) adult male 30day  (205) adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0000036 expression level Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr  (257) embryo 16-18hr  (464) embryo 18-20hr  (576) embryo 20-22hr  (466) embryo 22-24hr  (380) larva L1  (593) larva L2  120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr  116 white prepupae 24hr  (168) pupae 2d postWPP  (288) pupae 3d postWPP  (230) pupae 4d postWPP  111 adult male 01day  (153) adult male 05day  (221) adult male 30day  (205) adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2 embryo 02-04hr    0 embryo 04-06hr    1 embryo 06-08hr    4 embryo 08-10hr    6 embryo 10-12hr    30 embryo 12-14hr    72 embryo 14-16hr    257 embryo 16-18hr    464 embryo 18-20hr    576 embryo 20-22hr    466 embryo 22-24hr    380 larva L1    593 larva L2    120 larva L3 12hr old    47 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    74 larva L3 puffstage 7-9    73 white prepupae new    70 white prepupae 12hr    116 white prepupae 24hr    168 pupae 2d postWPP    288 pupae 3d postWPP    230 pupae 4d postWPP    111 adult male 01day    153 adult male 05day    221 adult male 30day    205 adult female 01day    48 adult female 05day    11 adult female 30day    18 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0000036    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0000036 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0000036 expression level Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0000036 expression level Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    4.725 Larval Midgut    3.7 Larval Hindgut    4.8 Larval Malpighian Tubules    1.6 Larval Fat Body    3.2 Larval Salivary Gland    5.5 Larval Trachea    4.7 Larval Carcass    2.95 Adult Head    2.9 Adult Eye    2.075 Adult Brain    14.5 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    3.2 Adult Midgut    4 Adult Hindgut    2.3 Adult Malpighian Tubules    3.9 Adult Fat Body    6.4 Adult Salivary Gland    3.7 Adult Heart    0.725 Adult VirginFemale Spermatheca    1.5 Adult InseminatedFemale Spermatheca    2.8 Adult Ovary    0.9 Adult Testis    6.2 Adult Male Accessory Gland no informative data Adult Carcass    4.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion no informative data Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.9   NA  Eye    2.075   NA  Brain    14.5   4.725  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    3.2   3.7  Midgut    4   4.8  Hindgut    2.3   1.6  Malpighian Tubules    3.9   3.2  Fat Body    6.4   5.5  Salivary Gland    3.7   NA  Heart    0.725   4.7  Trachea    NA   NA  VirginFemale Spermatheca    1.5   NA  InseminatedFemale Spermatheca    2.8   NA  Ovary    0.9   NA  Testis    6.2   NA  Male Accessory Gland    no informative data   2.95  Carcass    4.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_14
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000036 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5610-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4pnr-MD237 nAcRα-96AaGD234 viable nAcRα-96AaEY09706 Sterility Allele fertile nAcRα-96AaEY09706 Other Phenotypes Allele chemical resistant nAcRα-96AaEMS1 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of nAcRα-96Aa Class Mutagen Stocks Known lesion nAcRα-96AaEY09706 P-element activity 1 -- nAcRα-96AaMI03525 1 -- nAcRα-96Aa2 0 -- nAcRα-96AaEMS1 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nAcRα-96Aa Class Mutagen Stocks Known lesion nAcRα-96AaGD16733 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes nAcRα-96AaGD234 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nAcRα-96AaJF03103 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nAcRα-96Aahs.PS in vitro construct - regulatory fusion 0 Yes nAcRα-96AaMtnA.PL in vitro construct - regulatory fusion 0 Yes nAcRα-96AaMtnA.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)crb87-5 (Perry et al., 2008)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-nAcRα-96Aa.S} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}nAcRα-96AaEY09706 NA miscellaneous insertions Mi{MIC}nAcRα-96AaMI03525 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
acetylcholine-activated cation-selective channel activity inferred from electronic annotation with InterPro:IPR002394 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Littleton and Ganetzky, 2000)
Biological Process
CV term Evidence References
ion transport inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006029, InterPro:IPR006201, InterPro:IPR018000 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
acetylcholine-gated channel complex inferred from sequence or structural similarity (Littleton and Ganetzky, 2000) integral to membrane inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR006201, InterPro:IPR018000 (FlyBase Curators et al., 2004-) integral to plasma membrane non-traceable author statement (Swiss-Prot Project Members, 1989.3.1) postsynaptic membrane inferred from electronic annotation with InterPro:IPR002394, InterPro:IPR018000 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nAcRα-96Aa allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0000036 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42918
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20712 Dere\GG12342 Dgri\GH18787 Dmoj\GI22677 Dper\GL21978 Dpse\GA27331 Dsec\GM23483 Dsim\GD18289 Dvir\GJ23404 Dwil\GK22821 Dyak\GE10796
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004006-PA Aedes aegypti (mosquito) AGAP002974-PA Anopheles gambiae (mosquito) XP_001121970.1 Apis mellifera (honey bee) XP_001951542.1 Acyrthosiphon pisum (pea aphid) BGIBMGA012446-PA Bombyx mori (silk worm) CE24908 Caenorhabditis elegans (worm) CPIJ007643 Culex pipens (mosquito) 43617 Daphnia pulex (daphnia) ENSGACP00000006142 Gymnopilus aculeatus (mushroom) ENSGACP00000023421 Gymnopilus aculeatus (mushroom) 69301 Hypsipyla robusta (cedar shoot borer) 78292 Hypsipyla robusta (cedar shoot borer) ISCW003334-PA Ixodes scapularis (deer tick) XP_001607920.1 Nasonia vitripennis (wasp) ENSORLP00000001058 Oryzias latipes (japanese killifish) ENSORLP00000011046 Oryzias latipes (japanese killifish) PHUM005787-PA Pediculus humanus (human body louse) NP_001103245.1 Tribolium castaneum (red flour beetle) ENSTNIP00000000860 Tetraodon nigroviridis (green spotted pufferfish) ENSTNIP00000006787 Tetraodon nigroviridis (green spotted pufferfish) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10246 FBgn0228637 FBgn0224783 FBgn0210505 FBgn0189822 FBgn0178350 JGI_V11_43617 FBgn0248698 FBgn0159571 FBgn0145405 FBgn0126254 FBgn0104632 FBgn0097718 CPIJ007643 ACYPI008734 GB17133 AGAP002974 AAEL004006 PHUM114690 GLEAN_01018
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	28688 y1 v1; P{TRiP.JF03103}attP2 20083 y1 w67c23; P{EPgy2}nAcRα-96AaEY09706 36404 y1 w*; Mi{MIC}nAcRα-96AaMI03525/TM3, Sb1 Ser1
VDRC	v1189 w1118 P{GD234}v1189 v48159 w1118; P{GD16733}v48159 v48162 w1118; P{GD16733}v48162
Genomic Clones ( 2 )
	BACR04E17 BACR13M04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 4 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM02839 RH42472 RH67644
Other clones	DMG2-chr3R.21.049.a
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000036 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42918
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: nAcRα-96Aa CG5610 (Kodira, 1999.11)
Source for database merge of
Additional comments	"nAcRα-96Ab" is a putative chimeric gene derived from the "nAcRα-96Aa" and "nAcRβ-64B" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene). (Rogers et al., 2009)
Other Comments
	The distribution of nAcRα-96Aa protein in the developing and differentiated nervous system has been studied. (Schuster et al., 1993) Antibodies raised against fusion constructs encompassing specific regions of the Acr64B and Acr96Aa proteins were used to investigate a potential association of these two polypeptides. (Schloss et al., 1991) An alpha-like subunit that resemble ligand-binding subunits of vertebrate subunits. (Baumann et al., 1990) Characterisation of toxin binding to the domain carrying the cholinergic binding sites of nAcRα-96Aa. (Ohana and Gershoni, 1990) Structural gene encoding a Drosophila homologue of a subunit of vertebrate nicotinic acetylcholine receptors (nAChR); inferred to be homologous to neuronal α subunits based on the cystein doublet at amino-acid residues 201 and 202.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079757 RefSeq (Proteins) • NP_524481 DDBJ / EMBL / GenBank • AAF56301 CAA30172 CL706578 X07194 Entrez Gene - A searchable database of RefSeq genes. • 42918 UniProtKB/Swiss-Prot • P09478
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Neurotransmitter-gated ion-channel, conserved site (IPR018000) Nicotinic acetylcholine receptor (IPR002394) Neurotransmitter-gated ion-channel transmembrane domain (IPR006029) Neurotransmitter-gated ion-channel (IPR006201) Neurotransmitter-gated ion-channel ligand-binding (IPR006202)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0000036 DRSC - Results from RNAi screens. • FBgn0000036 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000036 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5610-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000036 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42918 InParanoid A subset of ortholog calls from InParanoid. • AAEL004006-PA Aedes aegypti (mosquito) AGAP002974-PA Anopheles gambiae (mosquito) XP_001121970.1 Apis mellifera (honey bee) XP_001951542.1 Acyrthosiphon pisum (pea aphid) BGIBMGA012446-PA Bombyx mori (silk worm) CE24908 Caenorhabditis elegans (worm) CPIJ007643 Culex pipens (mosquito) 43617 Daphnia pulex (daphnia) ENSGACP00000006142 Gymnopilus aculeatus (mushroom) ENSGACP00000023421 Gymnopilus aculeatus (mushroom) 69301 Hypsipyla robusta (cedar shoot borer) 78292 Hypsipyla robusta (cedar shoot borer) ISCW003334-PA Ixodes scapularis (deer tick) XP_001607920.1 Nasonia vitripennis (wasp) ENSORLP00000001058 Oryzias latipes (japanese killifish) ENSORLP00000011046 Oryzias latipes (japanese killifish) PHUM005787-PA Pediculus humanus (human body louse) NP_001103245.1 Tribolium castaneum (red flour beetle) ENSTNIP00000000860 Tetraodon nigroviridis (green spotted pufferfish) ENSTNIP00000006787 Tetraodon nigroviridis (green spotted pufferfish) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42918 modMine - Data generated by the modENCODE project. • FBgn0000036 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV10246 FBgn0228637 FBgn0224783 FBgn0210505 FBgn0189822 FBgn0178350 JGI_V11_43617 FBgn0248698 FBgn0159571 FBgn0145405 FBgn0126254 FBgn0104632 FBgn0097718 CPIJ007643 ACYPI008734 GB17133 AGAP002974 AAEL004006 PHUM114690 GLEAN_01018
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	Acr96A   Acr96Aa (Ranz et al., 1999, Ruiz et al., 1997, Ranz et al., 1997, ) AcrB   ALS (Lansdell and Millar, 2000, Lansdell and Millar, 2002, Schulz et al., 2000, Schulz et al., 1997, Hermsen et al., 1998, Schulz et al., 1998, Tomizawa and Casida, 1997, Schulz et al., 1997, Sgard et al., 1993, Lansdell et al., 1997, Tomizawa et al., 1996, Gundelfinger, 1992, Thomas et al., 1995, Gundelfinger et al., 1995, Schuster et al., 1993, Nedlina-Chittka and Gundelfinger, 1993, Gundelfinger et al., 1993, Bertrand et al., 1994, Schuster et al., 1993, Gundelfinger et al., 1991, Lansdell and Millar, 2000, Delpech et al., 2003) als (Pereira et al., 1997, Schmitt et al., 1989, Buchner, 1991, Schloss et al., 1991, ) ALs (Broughton et al., 1996) CG5610 (Bhutkar et al., 2008, Perkins et al., 2009) CG5610-PA (Rogers et al., 2009) dALS (Arquier et al., 2007, Hoshizaki and Gibbs, 2007) Dα1 (Perry et al., 2008) nAChR (Wolf and Heberlein, 2003, Tomizawa et al., 2003, Schafer, 2002) nAcr96Aa (Ranz et al., 2001) nAcRalpha-96Aa (Bhutkar et al., 2008) nAcRs (Ranz et al., 2001) nAcRα-96Aa (Rogers et al., 2009, Pym et al., 2006) α1 (Hermsen et al., 1998) α-like (Tsunoyama and Gojobori, 1998)
Name Synonym	alpha-like subunit (Gundelfinger, 1992, ) ALS (Sattelle et al., 2005) Dα1 (Sattelle et al., 2005) nicotinic Acetylcholine Receptor alpha 96Aa   nicotinic acetylcholine receptor alpha-like (Bossy, 1997.11.8) α-like-subunit   α-Like Subunit
Secondary FlyBase IDs
References ( 70 )
Generate a list of	All references relating to this gene ( 70 )
List References by type	Research paper  ( 32 ) Supplementary material  ( 3 ) Review  ( 9 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 12 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Book  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
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