Dα2, SAD, nAChR, nAcRα-96Ab, nAcRalpha-96Ab
Gene model reviewed during 5.47
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nAChRα2 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\nAChRα2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
"nAcRα-96Ab" is a putative chimeric gene derived from the "nAcRα-96Aa" and "nAcRβ-64B" genes (where coding sequences of the two parental genes contribute to the coding sequence of the chimeric gene).
Ecol\lacZ reporter gene constructs demonstrate the nAcRα-96Ab promoter is complex. A 1.23kb genomic promoter harbours the essential cis-elements for basic expression. Additional enhancer elements for strong expression in the optic lobe tangential cells are located 1.7 to 7.3kb upstream of the transcription start site.
Promoter region of nAcRα-96Ab is dissected and functional regions are identified.
Ecol\lacZ reporter gene constructs have been used to identify at least two elements necessary for regulated expression. One element is a 350bp region 1kb upstream of the putative transcription start site and contains a 37bp element that has high homology to element A2 of Ddc enhancer (Johnson et al, Genes and Devel. 3: 676).
The predicted mature protein contains 535 amino acid residues and an Mr of 60,963. It has an unusually long signal sequence of 41 residues.
The structure and developmental expression of Acr96Ab, a member of the nAChR gene family, has been characterized.
Northern blot analysis was used to determine the developmental expression of Acr96Ab and in situ hybridization revealed localization of Acr96Ab in the embryonic CNS.