Gene Dmel\Act5C

General Information
Symbol	Dmel\Act5C	Species	D. melanogaster
Name	Actin 5C	Annotation symbol	CG4027
Feature type	protein_coding_gene	FlyBase ID	FBgn0000042
Created / Updated	2005-01-10/2005-01-10
Genomic Location
Chromosome (arm)	X	Recombination map	1-
Cytogenetic map	5C7-5C7	Sequence location	X:5,794,897..5,798,823 [+]
Map ( GBrowse )
Summary Information
Automatically generated summary See sections below for more information	The gene Actin 5C is referred to in FlyBase by the symbol Act5C (CG4027, FBgn0000042). It has the cytological map location 5C7. Its sequence location is X:5794897..5798823. Its molecular function is described as: structural constituent of cytoskeleton; neurotransmitter transporter activity; ATP binding; protein binding. It is involved in the biological processes: cytoskeleton organization and biogenesis; sperm individualization; cytokinesis; phagocytosis, engulfment; neurotransmitter transport; visual behavior. 47 alleles are reported. The phenotypes of these alleles are annotated with: arista; trichome; eye; anterior crossvein; macrochaeta; germline cyst. It has 4 annotated transcripts and 4 annotated polypeptides.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	Act5C: Actin in region 5C Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (Fyrberg, Mahaffy, Bond, and Davidson, 1983, Cell 33: 115-23). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (Peterson, Bond, Mitchell, and Davidson, 1985, Dev. Genet. 5: 219-25). Transcripts present in the preblastoderm embryo suggesting maternal transcription; during blastoderm formation, Act5C transcripts accumulate at the periphery of the embryo; local concentrations of transcript observed in anterior and posterior midgut rudiments in stage 13 embryos; hybridization also observed in the developing ventral nervous system. Later in embryogenesis, transcript found in all tissues but with dramatic concentrations of transcripts in the anterior and posterior midgut and the proventriculus. Exon specific probes demonstrate that transcripts containing exon 1 tend to be concentrated in anterior portions of early embryos, including the anterior midgut primordium and the proventriculus, as well as in the posterior midgut primordium; during germ-band extension, small local concentrations of exon 2 transcripts are seen in posterior and ventral regions of the embryo (Burn, Vigoreaux, and Tobin, 1989, Dev. Biol. 131: 345-55).
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 5C7-5C7 Limits computationally determined from genome sequence between P{EP}CG15770^EP390 and P{EP}Act5C^EP1604&P{EP}EP444
Experimentally Determined Cytological Location
Cytogenetic map Notes References 5C3-5C6 (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation) 5D1--2 (determined by in situ hybridisation) 5C3--8 (determined by in situ hybridisation) 5C5--6 (determined by in situ hybridisation) 5C5--10 (determined by in situ hybridisation) (Peter et al., 2002) 5C5-5C10 (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation) (Schaefer et al., 1999.12.2) 5C3-5C8 (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation) (Schaefer et al., 1999.8.12) 5C5-5C8 (determined by in situ hybridisation) 5C5--6 (determined by in situ hybridisation) 5C3--9 (determined by in situ hybridisation) 5D1--2 (determined by in situ hybridisation) (Schaefer et al., 1999.6.15) 5C3-5C6 (determined by in situ hybridisation) 5C5--8 (determined by in situ hybridisation) (Schaefer et al., 1999.3.29) 5C-5C (determined by in situ hybridisation) (Vieira et al., 1997) 5C-5C (determined by in situ hybridisation) (Drosopoulou et al., 1997) 5C-5C (determined by in situ hybridisation) (Vigoreaux and Tobin, 1987) 5C-5C (determined by in situ hybridisation) (Fyrberg et al., 1980)
Experimentally Determined Recombination Data
Location	1- (Schaefer et al., 1999.3.29)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Act5C for information on other features
Comments on Gene Model

Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Act5C-RA FBtr0070822 NM_167053 1638 376 Act5C-RB FBtr0070823 NM_078497 1605 376 Act5C-RC FBtr0100662 NM_001014726 2251 376 Act5C-RD FBtr0100663 NM_001014725 1928 376
Additional Transcript Data & Comments
Reported size (kB)	1.97 (northern blot) (Rao and Sodja, 1992) 1.9, 1.77 (northern blot) (Rao et al., 1988)
Comments	These transcripts contain exon 2 and not exon 1. Between 9 and 15 transcripts are made from the Act5C gene with the use of two alternative leader exons and several poly A sites. Use of a particular 5\\' exon correlates with body distribution in embryos while poly A site selection does not. (Burn et al., 1989) These transcripts contain exon 1 and not exon 2. Between 9 and 15 transcripts are made from the Act5C gene with the use of two alternative leader exons and several poly A sites. Use of a particular 5\\' exon correlates with body distribution in embryos while poly A site selection does not. (Burn et al., 1989) Nuclear run-on transcription assays demonstrate that modulation of transcription frequency by controlling the escape of RNA polymerase II from a step early in transcript elongation may be a common mechanism in gene regulation. (Rougvie and Lis, 1990)
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Act5C-PA FBpp0070787 41.8 376 5.16 NP_727048 AAF46098 Act5C-PB FBpp0070788 41.8 376 5.16 NP_511052 AAN09154 Act5C-PC FBpp0100124 41.8 376 5.16 NP_001014726 AAX52479 Act5C-PD FBpp0100125 41.8 376 5.16 NP_001014725 AAX52480
Additional Polypeptide Data & Comments
Reported size (kD)
Comments	The brm core complex appears to comprise 10 polypeptides. These include brm, mor, osa, Bap60, Snr1, dalao, Bap55, Bap170, Bap26,and Act42A or Act5C proteins. (Kal et al., 2000) The BRM complex was purified from Drosophila embryos using two distinct purification protocols and the subunit composition was determined. Eight major proteins were consistently observed including brm, Snr1, Bap55 (a novel actin-related protein), Bap60, dalao, Hsc70-4, and mor proteins. The complex also contains a cytoplasmic actin (Act42A or Act5C). (Papoulas et al., 1998)
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0000042
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Actin/actin-like (IPR004000) Actin, conserved site (IPR004001) PDB - Protein Data Bank. An information portal to biological macromolecular structures • 1N58 TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles • R00047 R02467 R02597
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201888 AA202624 AA202678 AA202681 AA202728 AA246618 AA390524 AA391359 AA392602 AA392897 AA392977 AA433252 AA439790 AA440206 AA541118 AA541173 AA567505 AA695446 AA696327 AA696813 AA698818 AA803009 AA803011 AA804043 AA816347 AA821053 AA951701 AE003435 AAF46098 AE003435 AAN09154 AI260582 AI295837 AI513782 AI515313 AI944685 AJ426695 AJ426697 AJ426698 AJ426699 AJ426701 AJ426702 AJ426703 AJ426704 AJ426705 AQ025532 AQ073196 AQ073217 AQ073218 AQ074128 AQ254778 AY089562 AAL90300 BF485817 BF497293 BF498935 BF505185 BG636325 BG636432 BG637515 BG640326 BG640984 BG641184 BH614929 BI141719 BI141933 BI141949 BI142108 BI161512 BI162051 BI162274 BI162742 BI162958 BI163508 BI163726 BI166619 BI167498 BI169371 BI170039 BI170503 BI173337 BI173813 BI212461 BI212682 BI212799 BI369403 BI484288 BI576149 BI581049 BI588331 BI631310 BP541813 BP542980 BP542981 BP544793 BP544794 BP548390 BP548400 BP548478 BP549373 BP549374 BP553780 BP553781 BP555013 BP555014 BP555468 BP555469 BP556362 BP557892 BP557893 BQ102875 BQ103094 BT030949 CA803184 CL868538 CO155609 CO181729 CO185846 CO188627 CO193290 CO273952 CO275059 CO277305 CO280849 CO281947 CO295096 CO320511 CO334380 CO334393 CO334533 CO334544 CO334663 CO334670 CO334707 CO334712 CO334830 CO334859 CO334878 CO334888 CO334897 CO334981 CO335028 CO335152 CO335160 CO335274 CO335292 CO343052 CO343991 CZ471464 CZ472939 CZ477830 EC061930 EC063560 EC067824 EC069777 EC075546 EC090073 EC090695 EC194421 EC195093 EC197045 EC197488 EC198117 EC203990 EC211298 EC222752 EC228527 EC239014 EC240476 EC240570 EC241140 EC248293 EC254923 EC257311 EC259631 EC261551 EC263723 EC263894 EC264012 EC266269 EL875944 ES688829 ES689033 ES689114 ES691530