FlyBase Gene Report: Dmel\Act42A

General Information

Symbol

Dmel\Act42A

Species

D. melanogaster

Name

Actin 42A

Annotation Symbol

CG12051

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000043

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. (UniProt, P02572)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

actin, F-actin, BAP47, Act42, C

Key Links

Genomic Location

Cytogenetic map

42A7-42A7

Sequence location

2R:6,013,910..6,015,689 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:6013910..6015689

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in ovarian fusome organization

inferred from expression pattern

(Rodesch et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mitotic cytokinesis

inferred from biological aspect of ancestor with PANTHER:PTN002631617

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of brahma complex

inferred from direct assay

(Mohrmann et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN002631484

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ACTINS

BRAHMA ASSOCIATED PROTEINS COMPLEX

POLYBROMO-CONTAINING BRAHMA ASSOCIATED PROTEINS COMPLEX

Protein Family (UniProt)

Belongs to the actin family. (P02572)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ACTINS -: Actins are highly conserved eukaryotic filament-forming proteins that are major components of the cytoskeleton. Actin polymerisation is controlled by ATP binding and hydrolysis. Actins are involved in various cell processes including cell movement, muscle contraction, cell trafficking and mechanical support. (Adapted from PMID:21859859).
BRAHMA ASSOCIATED PROTEINS COMPLEX -: The Brahma associated proteins (BAP) complex is an ATP-dependent chromatin remodeling complex. (Adapted from FBrf0192510.)
POLYBROMO-CONTAINING BRAHMA ASSOCIATED PROTEINS COMPLEX -: Brahma (BRM) complexes are ATP-dependent chromatin remodeling complexes. The Polybromo-containing Brahma associated proteins (PBAP) complex is distinguished by the presence of polybromo and Bap170 subunits. (Adapted from FBrf0192510.)

Protein Function (UniProtKB)

(UniProt, P02572)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Act42A

Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (Fyrberg, Mahaffy, Bond, and Davidson, 1983, Cell 33: 115-23). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (Peterson, Bond, Mitchell, and Davidson, 1985, Dev. Genet. 5: 219-25).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Act42A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P02572)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Act42A-RA

FBtr0086029

NM_078901

1626

376

Additional Transcript Data and Comments

Reported size (kB)

1.72 (northern blot)

(Tobin et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Act42A-PA

FBpp0085365

41.8

376

5.16

P02572

NP_523625

AAF57294

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Post Translational Modification

N-terminal cleavage of acetylated cysteine of immature actin by ACTMAP.

Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

(UniProt, P02572)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P02572

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Act42A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Act42A_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.65

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

supraesophageal ganglion

(Tobin et al., 1990)

embryonic/larval midgut

(Tobin et al., 1990)

gonad

(Tobin et al., 1990)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval proventriculus

(Tobin et al., 1990)

larval ventral nerve cord

(Tobin et al., 1990)

blastoderm stage

(Tobin et al., 1990)

Additional Descriptive Data

Act42A transcripts are most abundant in embryos. They are evenly distributed in preblastoderm embryos, becoming localized to the periphery at blastoderm formation. Later they are observed in the brain and ventral nerve cord as well as in the developing gonad, proventriculus, and midgut.

(Tobin et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Dorus et al., 2006)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of brahma complex

inferred from direct assay

(Mohrmann et al., 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Act42A in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Act42A

View exonic expression by tissue for Dmel\Act42A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35526/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000043

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000043

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000043

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12051

Flygut - An atlas of the Drosophila adult midgut

FBgn0000043

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Act42A

Transgenic constructs containing regulatory region of Act42A

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel7085

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PU

Act42A^UASp.GFP

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Act42A^GD1350

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Act42A^GD1350

partially lethal, with Scer\GAL4^tin.CΔ4

Act42A^GD1350

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

Act42A^GD1350

Other Phenotypes

Allele

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Act42A^GD1350

visible, with Scer\GAL4^pnr-MD237

Act42A^GD1350

Phenotype manifest in

Allele

chaeta | increased number, with Scer\GAL4^pnr-MD237

Act42A^GD1350

external sensory organ, with Scer\GAL4^dpp.blk1

Act42A^GD1350

macrochaeta | ectopic, with Scer\GAL4^dpp.blk1

mesonotum, with Scer\GAL4^pnr-MD237

Act42A^GD1350

trichogen cell, with Scer\GAL4^pnr-MD237

Act42A^GD1350

wing sensillum, with Scer\GAL4^dpp.blk1

Act42A^GD1350

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (43)

Hsap\ACTB

10 of 14

Yes

Hsap\ACTG1

10 of 14

Yes

Hsap\ACTA1

7 of 14

Yes

Hsap\ACTBL2

7 of 14

Yes

Hsap\ACTC1

7 of 14

Yes

Hsap\ACTA2

6 of 14

Yes

Hsap\ACTG2

6 of 14

Hsap\POTEI

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

Hsap\POTEKP

4 of 14

Yes

Hsap\ACTL6A

3 of 14

Hsap\ACTL6B

3 of 14

Hsap\ACTL8

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\POTEB2

3 of 14

Yes

Hsap\POTEB3

3 of 14

Yes

Hsap\POTEB

3 of 14

Yes

Hsap\POTEC

3 of 14

Yes

Hsap\POTED

3 of 14

Yes

Hsap\POTEF

3 of 14

Hsap\POTEG

3 of 14

Yes

Hsap\POTEH

3 of 14

Yes

Hsap\POTEM

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\ACTR8

2 of 14

Hsap\ACTR10

2 of 14

Hsap\LOC102723502

2 of 14

Yes

Hsap\ACTR5

1 of 14

Hsap\POTEA

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Actb

11 of 14

Yes

Rnor\Actg1

10 of 14

Rnor\LOC100361457

10 of 14

Yes

Rnor\Acta1

7 of 14

Yes

Rnor\Actbl2

7 of 14

Yes

Rnor\Actc1

7 of 14

Yes

Rnor\Actg2

7 of 14

Rnor\Acta2

6 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Actl11

1 of 14

Rnor\Actr5

1 of 14

Rnor\LOC302827

1 of 14

Yes

Mus musculus (laboratory mouse) (29)

Mmus\Actb

11 of 14

Yes

Mmus\Actg1

11 of 14

Yes

Mmus\Acta1

7 of 14

Yes

Mmus\Acta2

7 of 14

Yes

Mmus\Actbl2

7 of 14

Yes

Mmus\Actc1

7 of 14

Yes

Mmus\Actg2

7 of 14

Mmus\Acte1

4 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm17087

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (15)

Xtro\actb

9 of 13

Yes

Xtro\actg1

9 of 13

Yes

Xtro\acta1

5 of 13

Yes

Xtro\acta4

5 of 13

Yes

Xtro\actc1

5 of 13

Yes

Xtro\MGC75582

4 of 13

Yes

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (23)

Drer\actb1

11 of 14

Yes

Drer\actb2

11 of 14

Yes

Drer\acta1a

7 of 14

Yes

Drer\acta1b

7 of 14

Yes

Drer\actc1a

7 of 14

Yes

Drer\actc2

7 of 14

Yes

Drer\acte1

7 of 14

Drer\acta2

6 of 14

Drer\actc1b

6 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

Drer\actr10

2 of 14

Drer\si:ch211-241j12.3

2 of 14

Drer\actr5

1 of 14

Drer\actr8

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\act-1

11 of 14

Yes

Cele\act-2

11 of 14

Yes

Cele\act-3

11 of 14

Yes

Cele\act-4

10 of 14

Cele\act-5

9 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Cele\swsn-6

3 of 14

Cele\arp-11

2 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP001676

6 of 12

Yes

Agam\AgaP_AGAP005095

6 of 12

Yes

Agam\AgaP_AGAP011515

6 of 12

Yes

Agam\AgaP_AGAP011516

6 of 12

Yes

Agam\ACT5C_ANOGA

5 of 12

Agam\AgaP_AGAP011514

5 of 12

Yes

4 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\ACT2

12 of 13

Yes

Atha\ACT7

12 of 13

Yes

Atha\ACT8

12 of 13

Yes

Atha\ACT11

12 of 13

Yes

11 of 13

11 of 13

11 of 13

11 of 13

6 of 13

Yes

Atha\ACT9

5 of 13

Yes

Atha\AT2G42170

5 of 13

Yes

Atha\ARP6

4 of 13

Atha\ARP7

4 of 13

Yes

Atha\ARP4

3 of 13

Atha\ARP8

3 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\ACT1

12 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Scer\ARP8

2 of 13

Scer\ARP5

1 of 13

Scer\ARP9

1 of 13

Schizosaccharomyces pombe (Fission yeast) (11)

Spom\act1

11 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Act42A. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (14)

12 of 13

11 of 13

11 of 13

11 of 13

11 of 13

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 11 )

Human Ortholog

Disease

Evidence

References

ACTB; actin beta

model of Baraitser-Winter syndrome 1

IEA

(FlyBase, 2019-)

ACTG1; actin gamma 1

model of autosomal dominant nonsyndromic deafness 20

IEA

(FlyBase, 2019-)

model of Baraitser-Winter syndrome 2

IEA

(FlyBase, 2019-)

ACTA1; actin alpha 1, skeletal muscle

model of congenital myopathy 2B

IEA

(FlyBase, 2019-)

model of congenital myopathy 2C

IEA

(FlyBase, 2019-)

model of nemaline myopathy 3

IEA

(FlyBase, 2019-)

ACTC1; actin alpha cardiac muscle 1

model of hypertrophic cardiomyopathy 11

IEA

(FlyBase, 2019-)

model of dilated cardiomyopathy 1R

IEA

(FlyBase, 2019-)

model of atrial heart septal defect 5

IEA

(FlyBase, 2019-)

ACTA2; actin alpha 2, smooth muscle

model of Moyamoya disease

IEA

(FlyBase, 2019-)

model of thoracic aortic aneurysm

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Act42A^GD1350

exacerbates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Act42A^EY05608

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ACTB; actin beta

10 of 14

102630

Baraitser-Winter syndrome 1

ACTG1; actin gamma 1

10 of 14

102560

ACTA1; actin alpha 1, skeletal muscle

7 of 14

102610

ACTBL2; actin beta like 2

7 of 14

614835

ACTC1; actin alpha cardiac muscle 1

7 of 14

102540

ACTA2; actin alpha 2, smooth muscle

6 of 14

102620

ACTG2; actin gamma 2, smooth muscle

6 of 14

102545

megacystis-microcolon-intestinal hypoperistalsis syndrome

POTEI; POTE ankyrin domain family member I

5 of 14

ACTR1A; actin related protein 1A

4 of 14

605143

ACTR1B; actin related protein 1B

4 of 14

605144

ACTRT3; actin related protein T3

4 of 14

608534

POTEE; POTE ankyrin domain family member E

4 of 14

608914

POTEJ; POTE ankyrin domain family member J

4 of 14

POTEKP; POTE ankyrin domain family member K, pseudogene

4 of 14

611266

ACTL6A; actin like 6A

3 of 14

604958

ACTL6B; actin like 6B

3 of 14

612458

developmental and epileptic encephalopathy 76

3 of 14

3 of 14

SPERMATOGENIC FAILURE 53; SPGF53

spermatogenic failure 53

ACTR2; actin related protein 2

3 of 14

604221

ACTR3B; actin related protein 3B

3 of 14

ACTR3; actin related protein 3

3 of 14

604222

ACTR6; actin related protein 6

3 of 14

619729

ACTRT1; actin related protein T1

3 of 14

300487

ACTRT2; actin related protein T2

3 of 14

608535

Hsap\LOC100288966

3 of 14

POTEB2; POTE ankyrin domain family member B2

3 of 14

POTEB3; POTE ankyrin domain family member B3

3 of 14

POTEB; POTE ankyrin domain family member B

3 of 14

608912

POTEC; POTE ankyrin domain family member C

3 of 14

POTED; POTE ankyrin domain family member D

3 of 14

607549

POTEF; POTE ankyrin domain family member F

3 of 14

POTEG; POTE ankyrin domain family member G

3 of 14

608916

POTEH; POTE ankyrin domain family member H

3 of 14

608913

POTEM; POTE ankyrin domain family member M

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Act42A - Act57B

two hybrid

(Hu et al., 2017.6.13)

Act42A - Act79B

protein cross-linking with a bifunctional reagent, Identification by mass spectrometry

(Mashtalir et al., 2018)

Act42A - Act87E

two hybrid

(Hu et al., 2017.6.13)

Act42A - Act88F

two hybrid

(Hu et al., 2017.6.13)

Act42A - CG7054

bimolecular fluorescence complementation, fluorescence microscopy

(Känel et al., 2022)

Act42A - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

Act42A - Pi3K21B

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Act42A - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Act42A - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Act42A - ac

anti tag coimmunoprecipitation, western blot, peptide massfingerprinting, two hybrid

(Hsiao et al., 2014)

Act42A - brm

anti bait coimmunoprecipitation, ion exchange chromatography, molecular sieving, molecular weight estimation by staining, pull down, edman degradation, peptide massfingerprinting, protein cross-linking with a bifunctional reagent, Identification by mass spectrometry

(Mashtalir et al., 2018, Mohrmann et al., 2004, Kal et al., 2000, Papoulas et al., 1998)

Act42A - capt

two hybrid, anti bait coimmunoprecipitation, western blot

(Hu et al., 2017.6.13, Wills et al., 2002)

Act42A - cbc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Wu et al., 2021)

Act42A - da

anti tag coimmunoprecipitation, western blot

(Hsiao et al., 2014)

Act42A - mor

far western blotting, autoradiography

(Kal et al., 2000)

Act42A - osk

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Hurd et al., 2016)

Act42A - otu

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Ji et al., 2019)

Act42A - sc

anti tag coimmunoprecipitation, western blot, two hybrid

(Hsiao et al., 2014)

Act42A - scra

anti tag coimmunoprecipitation, peptide massfingerprinting

(D'Avino et al., 2008)

Act42A - sti

pull down, western blot

(Bassi et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Act42A

(Hsiao et al., 2014)

Act5C

suppressible

Act42A

(Wagner et al., 2002)

Syx1A

suppressible

Act42A

(van Swinderen and Greenspan, 2005)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61432

DroID - A comprehensive database of gene and protein interactions.

FBgn0000043

MIST (genetic) - An integrated Molecular Interaction Database

35526

MIST (protein-protein) - An integrated Molecular Interaction Database

35526

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P02572

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42A7-42A7

Sequence location

2R:6,013,910..6,015,689 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42A7-42A7

Limits computationally determined from genome sequence between P{PZ}l(2)09851⁰⁹⁸⁵¹&P{lacW}Src42A^k10108 and P{lacW}l(2)k09848^k09848&P{EP}EP407

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42A-42A

(determined by in situ hybridisation)

(Drosopoulou et al., 1997, Fyrberg et al., 1981)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

9251

w^*; P{UASp-GFP.Act42A}1

9252

w^*; P{UASp-GFP.Act42A}5-5

15460

y¹ w^67c23; P{EPgy2}Act42A^EY05608

FlyORF

F001505

M{UAS-Act42A.ORF}ZH-86Fb

NIG-Fly

12051R-6

P{NIG.12051R}6

HMC02992

y¹ v¹; P{TRiP.HMC02992}attP2

VDRC

v104731

P{KK111854}VIE-260B

v12456

w¹¹¹⁸; P{GD1350}v12456

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (147)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD18090

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000043

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000043

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.

(Echard et al., 2004)

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Age related changes in Act42A expression have been studied by Northern analysis.

(Labuhn and Brack, 1997)

Ecdysteroid-regulated gene.

(Andres et al., 1993)

Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.

(Mounier et al., 1992)

Translation of actin RNA in early embryos injected with initiation factors has been studied.

(Hongo and Jacobs-Lorena, 1991)

The expression of HIS-C genes during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.

(Ruddell and Jacobs-Lorena, 1985)

Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (FBrf0038990). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (FBrf0042391). Transcription increased 6-8 fold by 20-OH-ecdysone in tissue culture cells (FBrf0047102; FBrf0064343).

Relationship to Other Genes

Source for database merge of

Member gene of

Actin

(Goldstein and Gunawardena, 2000)

Additional comments

The Act42A gene may be derived from the Act79B gene by retroposition.

(Betran et al., 2002)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

42A

(Cherbas et al., 2011)

(Courgeon et al., 1993)

ACT2

(Mashtalir et al., 2018)

ACT2_DROME

(Fukuyama et al., 2012)

ACT42A

(Känel et al., 2022)

AFFX-Dros-ACTIN_M_r_at

(Kristensen et al., 2005)

Act

(Wei et al., 2005)

Act42

(Chittaranjan et al., 2015, Blanco et al., 2010)

Act42A

(De Groef et al., 2024, Mori et al., 2024, Sutton et al., 2024, Ugrankar-Banerjee et al., 2023, McLaughlin et al., 2021, Xie et al., 2021, Jiang et al., 2019, Ji et al., 2019, Nithianandam and Chien, 2018, Hu et al., 2017.6.13, Min et al., 2017, Yoon et al., 2017, Chen et al., 2016, Tan et al., 2016, Chittaranjan et al., 2015, Dent et al., 2015, Kollmar, 2015.9.15, Sanhueza et al., 2015, Shiao et al., 2015, Zang et al., 2015, dos Santos, 2014, Hsiao et al., 2014, Kwon et al., 2013, Schertel et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Murray et al., 2012, Friedman et al., 2011, Gervais and Casanova, 2011, White et al., 2011, Carvalho et al., 2009, Liu et al., 2009, Venken et al., 2009, Bai et al., 2008, Fisher et al., 2008, Puri et al., 2008, Stuart et al., 2007, Dorus et al., 2006, Molnar et al., 2006, Montana and Littleton, 2006, Stroschein-Stevenson et al., 2006, Delanoue and Davis, 2005)

Act42a

(Schmid et al., 2024, Álvarez-Fernández et al., 2015, Bandyopadhyay et al., 2006, Earl and Britt, 2006)

Actin

(Schuettengruber et al., 2011, Clapier and Cairns, 2009, Balasubramanian et al., 2004, Eberharter and Becker, 2004, Niwa et al., 2004, Seegmiller et al., 2002, Quinn et al., 2001, Sisson et al., 2000, Zhang et al., 2000, Jones, 1999, Adams et al., 1998, Cooley, 1998, Hongo and Jacobs-Lorena, 1991, Stowe and Davis, 1990)

Actin/BAP47

(Mohrmann et al., 2004)

ArpB

(Kollmar and Odronitz, 2006)

BAP47

(Bouazoune and Brehm, 2006, Aalfs and Kingston, 2000, Kal et al., 2000, Papoulas et al., 1998)

(Bondinas et al., 2002, Bondinas et al., 2001)

CG12051

(Kolkhof et al., 2017, Hurd et al., 2016, Chittaranjan et al., 2015, Schertel et al., 2013, Christis and Munro, 2012, Ponton et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Liu et al., 2009, Hughes et al., 2008, Puri et al., 2008, Molnar et al., 2006, Verleyen et al., 2006, Seegmiller et al., 2002, Goldstein and Gunawardena, 2000)

act 42A

(Labuhn and Brack, 1997)

act42A

(Blatt et al., 2021, He and Haymer, 1995)

actin-42A

(Kwiatkowski et al., 2018)

Name Synonyms

42A actin

(Tobin, 1990.10.17)

Actin

(Ponton et al., 2011, Vorobyeva et al., 2009, Coutelis and Ephrussi, 2007, Griemert et al., 2007, Mulinari et al., 2007, Jaiswal et al., 2006, LaLonde et al., 2006, Zelhof et al., 2006, Zhai et al., 2006, He et al., 2005, Wei et al., 2005, Wills et al., 2002)

Actin 42A

(Hsiao et al., 2014, Liu et al., 2009, D'Avino et al., 2008)

Actin-42A

(Pflieger et al., 2008)

Actin42A

(Matamoro-Vidal et al., 2024, Ahrens et al., 2007, Norgate et al., 2007, Verleyen et al., 2006)

F-actin

(Kang et al., 2017, Huang et al., 2016, Xu et al., 2011, Cavey et al., 2008, Sasaki et al., 2007)

actin42A

(Solana-Manrique et al., 2021, Lesch and Page, 2012, Borghese et al., 2006)

actin42a

(Zheng et al., 2013)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 60 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35526

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P02572

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P02572

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P02572

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35526/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000043

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000043

FlyMine - An integrated database for Drosophila genomics

FBgn0000043

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG12051

MARRVEL_MODEL - MARRVEL (model organism gene)

35526

Linkouts

BioGRID - A database of protein and genetic interactions.

61432

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000043

DroID - A comprehensive database of gene and protein interactions.

FBgn0000043

DRSC - Results frm RNAi screens

FBgn0000043

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Act42A_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000043

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000043

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12051

Flygut - An atlas of the Drosophila adult midgut

FBgn0000043

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

35526

MIST (protein-protein) - An integrated Molecular Interaction Database

35526

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P02572

References (314)

The following external sites may use different assemblies or annotations than FlyBase.

0.65

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