FlyBase Gene Report: Dmel\Act87E

General Information

Symbol

Dmel\Act87E

Species

D. melanogaster

Name

Actin 87E

Annotation Symbol

CG18290

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000046

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Multiple isoforms are involved in various cellular functions such as cytoskeleton structure, cell mobility, chromosome movement and muscle contraction. (UniProt, P10981)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

actin, E

Key Links

Genomic Location

Cytogenetic map

87E11-87E11

Sequence location

3R:13,425,983..13,428,375 [+]

Recombination map

3-53

RefSeq locus

NT_033777 REGION:13425983..13428375

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in DNA repair-dependent chromatin remodeling

inferred from direct assay

(Kusch et al., 2004)

involved_in ovarian fusome organization

inferred from expression pattern

(Rodesch et al., 1997)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in mitotic cytokinesis

inferred from biological aspect of ancestor with PANTHER:PTN002631617

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in actin cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN002631484

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ACTINS

TIP60 COMPLEX

Protein Family (UniProt)

Belongs to the actin family. (P10981)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

ACTINS -: Actins are highly conserved eukaryotic filament-forming proteins that are major components of the cytoskeleton. Actin polymerisation is controlled by ATP binding and hydrolysis. Actins are involved in various cell processes including cell movement, muscle contraction, cell trafficking and mechanical support. (Adapted from PMID:21859859).
TIP60 COMPLEX -: The Tip60 chromatin remodeling complex acetylates nucleosomal phospho-histone H2Av and exchanges it with unmodified H2Av. (Adapted from FBrf0185742).

Protein Function (UniProtKB)

(UniProt, P10981)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Act87E

Encodes actin found in larval muscle and adult cephalic and abdominal muscle (Fyrberg, Mahaffey, Bond, and Davidson, 1983, Cell 33: 115-23).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Act87E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P10981)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.54

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Act87E-RA

FBtr0082785

NM_057743

1567

376

Act87E-RB

FBtr0082786

NM_169525

1643

376

Act87E-RC

FBtr0346242

NM_001300385

1853

376

Additional Transcript Data and Comments

Reported size (kB)

1.65 (northern blot)

(Tobin et al., 1990)

1.7 (northern blot)

(Manseau et al., 1988)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Act87E-PA

FBpp0082253

41.8

376

5.15

FBpp0082254

41.8

376

5.15

FBpp0312019

41.8

376

5.15

P10981

NP_001287314

AHN57313

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

376 aa isoforms: Act87E-PA, Act87E-PB, Act87E-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

376 (aa)

(Manseau et al., 1988)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Tip60 chromatin-remodeling complex which contains the catalytic subunit Tip60 and the subunits Domino, Tra1, Brd8, E(Pc), DMAP1, Pontin, Reptin, Ing3, Act87E, BAP55, Mrg15, MrgBP, Gas41 and YL-1.

(UniProt, P10981)

Post Translational Modification

N-terminal cleavage of acetylated cysteine of immature actin by ACTMAP.

Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.

(UniProt, P10981)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Act87E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Act87E_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.50

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | intense | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

foregut anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

head visceral muscle primordium

(Fisher et al., 2012)

longitudinal trunk visceral muscle primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

embryonic/larval esophageal visceral muscle

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

larval stage

larval muscle system

(Tobin et al., 1990)

adult stage

adult dorsal vessel

(Shah et al., 2011)

Additional Descriptive Data

Act87E expression is observed in the adult dorsal vessel but not in the larval dorsal vessel.

(Shah et al., 2011)

Act87E transcripts are most abundant in larvae. They accumulate in a small subset of larval segmentally repeated muscles. The level of transcript accumulation is much lower than that of Act57B transcripts.

(Tobin et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010, Dorus et al., 2006)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of NuA4 histone acetyltransferase complex

inferred from direct assay

(Kusch et al., 2004)

Expression Deduced from Reporters

Reporter: P{Act87E-lacZ.-344+6}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult heart

(Shah et al., 2011)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Act87E in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Act87E

View exonic expression by tissue for Dmel\Act87E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000046

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48632/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000046

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000046

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000046

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18290

Flygut - An atlas of the Drosophila adult midgut

FBgn0000046

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Act87E

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Act87E

Transgenic constructs containing regulatory region of Act87E

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(3R)Exel7320

(Ryder, 2004.4.26)

Df(3R)l26c

(Manseau et al., 1988)

Df(3R)ry619

(Manseau et al., 1988)

Gene not disrupted in

Df(3R)lC4a

(Manseau et al., 1988)

Df(3R)ry1168

(Manseau et al., 1988)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PU

Act87E^UASp.GFP

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (43)

Hsap\ACTB

9 of 14

Yes

Hsap\ACTA1

7 of 14

Yes

Hsap\ACTBL2

7 of 14

Yes

Hsap\ACTC1

7 of 14

Yes

Hsap\ACTG1

7 of 14

Hsap\ACTA2

6 of 14

Yes

Hsap\ACTG2

6 of 14

Hsap\ACTRT3

5 of 14

Yes

Hsap\ACTL9

4 of 14

Yes

Hsap\ACTR1A

4 of 14

Hsap\ACTR1B

4 of 14

Hsap\ACTRT1

4 of 14

Yes

Hsap\ACTRT2

4 of 14

Yes

Hsap\POTEE

4 of 14

Hsap\POTEI

4 of 14

Hsap\POTEJ

4 of 14

Yes

Hsap\POTEKP

4 of 14

Yes

Hsap\ACTL6A

3 of 14

Hsap\ACTL6B

3 of 14

Hsap\ACTL7A

3 of 14

Yes

Hsap\ACTL7B

3 of 14

Yes

Hsap\ACTL8

3 of 14

Yes

Hsap\ACTL10

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\POTEB2

3 of 14

Yes

Hsap\POTEB3

3 of 14

Yes

Hsap\POTEB

3 of 14

Yes

Hsap\POTEC

3 of 14

Yes

Hsap\POTED

3 of 14

Yes

Hsap\POTEF

3 of 14

Hsap\POTEG

3 of 14

Yes

Hsap\POTEH

3 of 14

Yes

Hsap\POTEM

3 of 14

Yes

Hsap\ACTR3C

2 of 14

Yes

Hsap\ACTR8

2 of 14

Hsap\ACTR10

2 of 14

Hsap\LOC102723502

2 of 14

Yes

Hsap\ACTR5

1 of 14

Hsap\POTEA

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Actb

10 of 14

Yes

Rnor\Acta1

7 of 14

Yes

Rnor\Actbl2

7 of 14

Yes

Rnor\Actc1

7 of 14

Yes

7 of 14

7 of 14

7 of 14

6 of 14

Yes

Rnor\Actrt3

5 of 14

Yes

Rnor\Actl9

4 of 14

Yes

Rnor\Actl10

4 of 14

Yes

Rnor\Actr1a

4 of 14

Rnor\Actr1b

4 of 14

Rnor\Actrt1

4 of 14

Yes

Rnor\Actrt2

4 of 14

Yes

Rnor\Actl6a

3 of 14

Rnor\Actl6b

3 of 14

Rnor\Actl7a

3 of 14

Yes

Rnor\Actl7b

3 of 14

Yes

Rnor\Actl9b

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Rnor\Actl11

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Rnor\Potef

1 of 14

Mus musculus (laboratory mouse) (29)

Mmus\Actb

10 of 14

Yes

Mmus\Actg1

8 of 14

Mmus\Acta1

7 of 14

Yes

Mmus\Acta2

7 of 14

Yes

Mmus\Actbl2

7 of 14

Yes

Mmus\Actc1

7 of 14

Yes

Mmus\Actg2

7 of 14

Mmus\Actrt3

5 of 14

Yes

Mmus\Acte1

4 of 14

Yes

Mmus\Actl9

4 of 14

Yes

Mmus\Actl10

4 of 14

Yes

Mmus\Actr1a

4 of 14

Mmus\Actr1b

4 of 14

Mmus\Actrt1

4 of 14

Yes

Mmus\Actrt2

4 of 14

Yes

Mmus\Actl6a

3 of 14

Mmus\Actl6b

3 of 14

Mmus\Actl7a

3 of 14

Yes

Mmus\Actl7b

3 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Mmus\Gm17087

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (15)

Xtro\actb

8 of 13

Yes

Xtro\actg1

7 of 13

Xtro\acta1

5 of 13

Yes

Xtro\acta4

5 of 13

Yes

Xtro\actc1

5 of 13

Yes

Xtro\MGC75582

4 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (23)

Drer\actb1

9 of 14

Yes

Drer\actb2

9 of 14

Yes

Drer\acta1a

7 of 14

Yes

Drer\acta1b

7 of 14

Yes

Drer\actc1a

7 of 14

Yes

Drer\actc2

7 of 14

Yes

Drer\acte1

7 of 14

Drer\acta2

6 of 14

Drer\actc1b

6 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Drer\si:ch211-241j12.3

3 of 14

Yes

Drer\zgc:101810

3 of 14

Drer\actc1c

2 of 14

Yes

Drer\actr10

2 of 14

Drer\actr5

1 of 14

Drer\actr8

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\act-1

9 of 14

Yes

Cele\act-2

9 of 14

Yes

Cele\act-3

9 of 14

Yes

8 of 14

7 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Cele\swsn-6

3 of 14

Cele\arp-11

2 of 14

Anopheles gambiae (African malaria mosquito) (14)

Agam\AgaP_AGAP005095

12 of 12

Yes

7 of 12

6 of 12

6 of 12

5 of 12

5 of 12

Yes

4 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (20)

Atha\ACT2

12 of 13

Yes

Atha\ACT7

12 of 13

Yes

Atha\ACT8

12 of 13

Yes

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

6 of 13

Yes

Atha\ACT9

5 of 13

Yes

Atha\AT2G42170

5 of 13

Yes

Atha\ARP6

4 of 13

Atha\ARP7

4 of 13

Yes

Atha\ARP4

3 of 13

Atha\ARP8

3 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\ACT1

11 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Scer\ARP8

2 of 13

Scer\ARP5

1 of 13

Scer\ARP9

1 of 13

Schizosaccharomyces pombe (Fission yeast) (11)

Spom\act1

11 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Act87E. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (14)

13 of 13

12 of 13

12 of 13

11 of 13

11 of 13

6 of 13

5 of 13

5 of 13

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 11 )

Human Ortholog

Disease

Evidence

References

ACTB; actin beta

model of Baraitser-Winter syndrome 1

IEA

(FlyBase, 2019-)

ACTC1; actin alpha cardiac muscle 1

model of atrial heart septal defect 5

IEA

(FlyBase, 2019-)

model of hypertrophic cardiomyopathy 11

IEA

(FlyBase, 2019-)

model of dilated cardiomyopathy 1R

IEA

(FlyBase, 2019-)

ACTA1; actin alpha 1, skeletal muscle

model of congenital myopathy 2C

IEA

(FlyBase, 2019-)

model of nemaline myopathy 3

IEA

(FlyBase, 2019-)

model of congenital myopathy 2B

IEA

(FlyBase, 2019-)

ACTA2; actin alpha 2, smooth muscle

model of Moyamoya disease

IEA

(FlyBase, 2019-)

model of thoracic aortic aneurysm

IEA

(FlyBase, 2019-)

ACTL9; actin like 9

model of spermatogenic failure 53

IEA

(FlyBase, 2019-)

ACTL7A; actin like 7A

model of spermatogenic failure 86

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ACTB; actin beta

9 of 14

102630

Baraitser-Winter syndrome 1

ACTA1; actin alpha 1, skeletal muscle

7 of 14

102610

ACTBL2; actin beta like 2

7 of 14

614835

ACTC1; actin alpha cardiac muscle 1

7 of 14

102540

ACTG1; actin gamma 1

7 of 14

102560

ACTA2; actin alpha 2, smooth muscle

6 of 14

102620

ACTG2; actin gamma 2, smooth muscle

6 of 14

102545

megacystis-microcolon-intestinal hypoperistalsis syndrome

ACTRT3; actin related protein T3

5 of 14

608534

ACTL9; actin like 9

4 of 14

619251

SPERMATOGENIC FAILURE 53; SPGF53

spermatogenic failure 53

ACTR1A; actin related protein 1A

4 of 14

605143

ACTR1B; actin related protein 1B

4 of 14

605144

ACTRT1; actin related protein T1

4 of 14

300487

ACTRT2; actin related protein T2

4 of 14

608535

POTEE; POTE ankyrin domain family member E

4 of 14

608914

POTEI; POTE ankyrin domain family member I

4 of 14

POTEJ; POTE ankyrin domain family member J

4 of 14

POTEKP; POTE ankyrin domain family member K, pseudogene

4 of 14

611266

ACTL6A; actin like 6A

3 of 14

604958

ACTL6B; actin like 6B

3 of 14

612458

developmental and epileptic encephalopathy 76

ACTL7A; actin like 7A

3 of 14

604303

SPERMATOGENIC FAILURE 86; SPGF86

spermatogenic failure 86

ACTL7B; actin like 7B

3 of 14

604304

ACTL8; actin like 8

3 of 14

ACTL10; actin like 10

3 of 14

ACTR2; actin related protein 2

3 of 14

604221

ACTR3B; actin related protein 3B

3 of 14

ACTR3; actin related protein 3

3 of 14

604222

ACTR6; actin related protein 6

3 of 14

619729

Hsap\LOC100288966

3 of 14

POTEB2; POTE ankyrin domain family member B2

3 of 14

POTEB3; POTE ankyrin domain family member B3

3 of 14

POTEB; POTE ankyrin domain family member B

3 of 14

608912

POTEC; POTE ankyrin domain family member C

3 of 14

POTED; POTE ankyrin domain family member D

3 of 14

607549

POTEF; POTE ankyrin domain family member F

3 of 14

POTEG; POTE ankyrin domain family member G

3 of 14

608916

POTEH; POTE ankyrin domain family member H

3 of 14

608913

POTEM; POTE ankyrin domain family member M

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Act87E - Act42A

two hybrid

(Hu et al., 2017.6.13)

Act87E - Act79B

two hybrid

(Hu et al., 2017.6.13)

Act87E - Act88F

two hybrid

(Hu et al., 2017.6.13)

Act87E - Nrd1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Yoon et al., 2017)

Act87E - capt

two hybrid

(Hu et al., 2017.6.13)

Act87E - pont

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kusch et al., 2004)

Act87E - scra

anti tag coimmunoprecipitation, peptide massfingerprinting

(D'Avino et al., 2008)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

shot

suppressible

Act87E

(Sun et al., 2019)

External Data

Subunit Structure (UniProtKB)

(UniProt, P10981 )

Linkouts

BioGRID - A database of protein and genetic interactions.

71541

DroID - A comprehensive database of gene and protein interactions.

FBgn0000046

MIST (protein-protein) - An integrated Molecular Interaction Database

48632

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Smooth Muscle Contraction

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P10981

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-53

Cytogenetic map

87E11-87E11

Sequence location

3R:13,425,983..13,428,375 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87E11-87E11

Limits computationally determined from genome sequence between P{PZ}CtBP⁰³⁴⁶³ and P{lacW}B52^s2249&P{lacW}flfl^L4179

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

87E-87E

(determined by in situ hybridisation)

(Drosopoulou et al., 1997)

87E9-87E12

(determined by in situ hybridisation)

(Ranz et al., 1997)

Experimentally Determined Recombination Data

Location

3-53

(FlyBase, 2016)

3-50.9

(Comeron et al., 2012)

3-[53]

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (77)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000046

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000046

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Age related changes in Act87E expression have been studied by Northern analysis.

(Labuhn and Brack, 1997)

Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.

(Mounier et al., 1992)

Translation of actin RNA in early embryos injected with initiation factors has been studied.

(Hongo and Jacobs-Lorena, 1991)

No lethal mutations in Act87E gene were isolated during a molecular and genetic analysis of region including Act87E gene. Possible explanations include non-lethality of Act87E mutations or substitution of Act87E function by other actin-encoding genes in the genome.

(Manseau et al., 1988)

Encodes actin found in larval muscle and adult cephalic and abdominal muscle (FBrf0038990).

Relationship to Other Genes

Source for database merge of

Source for merge of: Act87E CG18290

(Gelbart, 2000.4.13)

Member gene of

Actin

(Goldstein and Gunawardena, 2000)

Additional comments

The Act87E gene may have been derived from the Act5C gene by retroposition.

(Pan and Zhang, 2009)

The Act87E gene may have been derived from the Act57B gene by retroposition.

(Bai et al., 2007)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

ACT5

(Liu et al., 2018)

Act87E

(Trujillo et al., 2024, Brener et al., 2023, Lee et al., 2023, Ugrankar-Banerjee et al., 2023, Logeay et al., 2022, Sebastian et al., 2022, Bordet et al., 2021, McLaughlin et al., 2021, Ogneva and Usik, 2021, Shieh et al., 2021, Usik et al., 2021, Poovathumkadavil and Jagla, 2020, Meltzer et al., 2019, McCarthy et al., 2018, Hu et al., 2017.6.13, Min et al., 2017, Yoon et al., 2017, Kupriyanova et al., 2016, Ogneva et al., 2016, Kollmar, 2015.9.15, Ma et al., 2015, Belozerov et al., 2014, dos Santos, 2014, Wang et al., 2014, Johnson et al., 2013, Schertel et al., 2013, Yoshigi et al., 2013, Aoki et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Friedman et al., 2011, Shah et al., 2011, Cunha et al., 2010, Wasbrough et al., 2010, Gelbart et al., 2009, Liu et al., 2009, Venken et al., 2009, Bai et al., 2008, Bai et al., 2007, Stuart et al., 2007, Dorus et al., 2006, Montana and Littleton, 2006, Jin, 2005, Kusch et al., 2004)

Act87e

(Bandyopadhyay et al., 2006)

Actin

(Schuettengruber et al., 2011, Clapier and Cairns, 2009, Balasubramanian et al., 2004, Eberharter and Becker, 2004, Jones, 1999, Hongo and Jacobs-Lorena, 1991)

Actin87E

(Clapier and Cairns, 2009)

ArpC

(Kollmar and Odronitz, 2006)

CG18290

(Meadows, 2018.10.31, Kolkhof et al., 2017, Çiçek et al., 2016, Schertel et al., 2013, Yoshigi et al., 2013, Aoki et al., 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Cermelli et al., 2006, Kusch et al., 2004, Gelbart, 2000.4.13, Goldstein and Gunawardena, 2000)

(Bondinas et al., 2002, Bondinas et al., 2001)

act 87E

(Labuhn and Brack, 1997)

act87E

(Blatt et al., 2021, Raghuraman et al., 2020, He and Haymer, 1995)

actin

(Eltsov et al., 2015, Rui et al., 2010, Padash Barmchi et al., 2005, Delmas et al., 2004, Kwon and Scholey, 2004, Strickland and Burgess, 2004, Xia and Karin, 2004, Jacinto and Baum, 2003, Miller, 2003, Baum, 2002, Biggin, 2001, Adams et al., 2000, Noselli and Agnes, 1999, OOta and Saitou, 1999, Bearer, 1991)

actin-87E

(Kwiatkowski et al., 2018)

mus87E

(White and Crother, 1999)

Name Synonyms

Actin

(Griemert et al., 2007, Mulinari et al., 2007, Jaiswal et al., 2006)

Actin 87E

(Hull et al., 2015, Wójtowicz et al., 2015, Cammarato et al., 2011, D'Avino et al., 2008)

F-actin

(Kang et al., 2017)

actin

(Davis et al., 2015, Jaiswal et al., 2013, Lenartowska et al., 2012, Bai et al., 2007, Costantino et al., 2007, Lo, 2006, Sordella and Van, 2006, Harris and Peifer, 2004, Zeitler et al., 2004, Baehrecke, 2003)

actin 87E

(Sen and Cox, 2016, Cermelli et al., 2006)

actin87E

(García-Zaragoza et al., 2008)

Secondary FlyBase IDs

FBgn0038154

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

48632

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P10981

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P10981

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4LH50

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P10981

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000046

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

48632/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0000046

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0000046

FlyMine - An integrated database for Drosophila genomics

FBgn0000046

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG18290

MARRVEL_MODEL - MARRVEL (model organism gene)

48632

Linkouts

BioGRID - A database of protein and genetic interactions.

71541

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000046

DroID - A comprehensive database of gene and protein interactions.

FBgn0000046

DRSC - Results frm RNAi screens

FBgn0000046

Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Act87E_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0000046

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000046

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG18290

Flygut - An atlas of the Drosophila adult midgut

FBgn0000046

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

48632

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Smooth Muscle Contraction

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P10981

References (175)

The following external sites may use different assemblies or annotations than FlyBase.

0.50

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