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FB2025_05
,
released December 11, 2025
ade3, adenosine 3
trifunctional polypeptide with the activities of phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, and phosphoribosylaminoimidazole synthetase - expressed in glia, fat body, and gut - positively regulates feeding behavior via cooperation and coordination - acting in the gut, Gart is crucial for maintaining endogenous feeding rhythms and food intake, while Gart in glia and fat body regulates energy homeostasis between synthesis and metabolism - directly regulated by the CLOCK/CYCLE heterodimer via canonical E-box
Please see the JBrowse view of Dmel\Gart for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
4.7, 1.7 (northern blot)
1353, 434 (aa)
>1296 (aa)
A shorter alternative form of Gart protein which lacks the C-terminal GAR-transformylase domain.
Homodimer.
The N-terminal ATP-grasp domain carries the phosphoribosylamine--glycine ligase activity.
The central AIRS domain carries the phosphoribosylformylglycinamidine cyclo-ligase activity.
The C-terminal GART domain carries the phosphoribosylglycinamide formyltransferase activity.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gart using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: low expression throughout adult brain
JBrowse - Visual display of RNA-Seq signals
View Dmel\Gart in JBrowse2-24
2-23.2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Encodes the three enzymes in the purine biosynthetic pathway, glycineamide ribotide synthetase (GARS), aminoimidazole ribotide synthetase (AIRS) and GART.
Purine nucleoside auxotroph supplementable with adenine, adenosine and inosine. Recovery of ade3 progeny from crosses between ade31 and ade31/SM5 is about 1% when raised on minimal medium. Less than 3% of the normal activity purine de novo synthetic pathway enzyme, glycineamide ribotide transformylase (GART). Mutants, if viable, have a brownish eye colour and wing and bristle defects.
Nucleotide excision repair of (6-4)photoproducts has been assayed in the ade3 locus of Kc (embryonic) cell line, after exposure to UV light. The ade3 locus is transcriptionally active in Kc cells, but the repair rate is the same as for the transcriptionally inactive white gene, as well as for the transcriptionally active N gene. Repair rate is enhanced with prior exposure to photoreactivating light. No strand specificity of repair is observed.
Removal of UV-induced pyrimidine dimers is measured in genes ade3, N and w in two diploid immortalised cell lines (Kc and SL2) to investigate whether preferential repair forms part of DNA excision repair. Data supports the idea that preferential repair is not restricted to transcriptionally active sequences.
The organization of the ade3 protein in Drosophila has multiple domains with GAR transformylase at the carboxyl terminus preceded by an extensive repeat.
Enzyme assays confirm that ade3 encodes GAR transformylase.
Annotation CG8761 split into CG31628 (ade3), CG31908 in release 3 of the genome annotation.
Source for identity of: Gart ade3
Renamed from 'ade3' to 'Gart' (in FB2018_01) to reflect usage in the literature and provide nomenclature consistent with the clear vertebrate ortholog (and replace existing nomenclature suggesting incorrect relationships to yeast orthologs).
