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Gene Dmel\ade3

General Information
Symbol	Dmel\ade3	Species	D. melanogaster
Name	adenosine 3	Annotation symbol	CG31628
Feature type	protein_coding_gene	FlyBase ID	FBgn0000053
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	Gart
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	27D4-27D4	Sequence location	2L:7,014,623..7,023,940 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene adenosine 3 is referred to in FlyBase by the symbol Dmel\ade3 (CG31628, FBgn0000053). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: phosphoribosylamine-glycine ligase activity; phosphoribosylformylglycinamidine cyclo-ligase activity. Based on sequence similarity, it is predicted to be involved in the biological process: 'de novo' IMP biosynthetic process. 19 alleles are reported. The phenotypes of these alleles are annotated with: ventral row; tarsal segment; dorsal row; wing; wing vein; macrochaeta; pigment cell; trichome of the posterior wing margin. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: AIR synthase related protein, N-terminal domain; AIR synthase-related protein, C-terminal domain; ATP-grasp fold; ATP-grasp fold, subdomain 1; ATP-grasp fold, subdomain 2; Formyl transferase, N-terminal; Phosphoribosylformylglycinamidine cyclo-ligase; Phosphoribosylglycinamide formyltransferase; Phosphoribosylglycinamide formyltransferase, active site; Phosphoribosylglycinamide synthetase; Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain; Phosphoribosylglycinamide synthetase, C-domain; Phosphoribosylglycinamide synthetase, N-terminal; Phosphoribosylglycinamide synthetase, conserved site; Pre-ATP-grasp domain; PurM, N-terminal-like; Rudiment single hybrid motif. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval Malpighian tubules, adult heart, adult fat body, larval salivary gland, adult ovary, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1637037_at completely aligns to an exonic region of only a subset of 1 of the 2 FlyBase-annotated transcript isoforms of ade3. Gene sequence location is 2L:7014623..7023940.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ade3 (S. Henikoff and D. Nash) Purine nucleoside auxotroph supplementable with adenine, adenosine, and inosine. Recovery of ade3 progeny from crosses between ade3 and ade3/SM5 is about 1% when raised on minimal medium. Less than 3% of the normal activity purine de novo synthetic pathway enzyme, glycineamide ribotide transformylase [EC 2.1.2.2 (GART)]. Eye color normal.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 27D4-27D4   Limits computationally determined from genome sequence between P{PZ}snRNP70K02107 and P{PZ}wg02657
Experimentally Determined Cytological Location
Cytogenetic map Notes References 27D-27D   (determined by in situ hybridisation)   (Henikoff et al., 1986) 27D-27D   (determined by in situ hybridisation)   (Henikoff et al., 1981)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ade3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ade3-RA FBtr0079431  NM_078773   4522   1353 ade3-RB FBtr0100353  NM_001014477   1598   434 ade3-RC FBtr0339663     4760   1353
Additional Transcript Data & Comments
Reported size (kB)	4.7, 1.7 (northern blot) (Henikoff et al., 1983)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ade3-PA   FBpp0079059   144.5   1353   7.22     NP_523497   AAF52474 ade3-PB   FBpp0099760   45.9   434   7.65     NP_001014477   AAX52661 ade3-PC   FBpp0308722   144.5   1353   7.22
Additional Polypeptide Data & Comments
Reported size (kDa)	>1296 (aa) (Henikoff et al., 1983) 1353, 434 (aa) (Henikoff et al., 1986)
Comments	A shorter alternative form of ade3 protein which lacks the C-terminal GAR-transformylase domain. (Henikoff et al., 1983)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • ATP-grasp fold, subdomain 1 (IPR013815) ATP-grasp fold, subdomain 2 (IPR013816) Phosphoribosylglycinamide synthetase (IPR000115) AIR synthase related protein, N-terminal domain (IPR000728) Phosphoribosylglycinamide formyltransferase, active site (IPR001555) Formyl transferase, N-terminal (IPR002376) Phosphoribosylglycinamide formyltransferase (IPR004607) Phosphoribosylformylglycinamidine cyclo-ligase (IPR004733) AIR synthase-related protein, C-terminal domain (IPR010918) Rudiment single hybrid motif (IPR011054) ATP-grasp fold (IPR011761) Pre-ATP-grasp domain (IPR016185) PurM, N-terminal-like (IPR016188) Phosphoribosylglycinamide synthetase, conserved site (IPR020559) Phosphoribosylglycinamide synthetase, C-domain (IPR020560) Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (IPR020561) Phosphoribosylglycinamide synthetase, N-terminal (IPR020562)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246309   AE014134 AAF52474   AE014134 AAX52661   AI063415   AI064421   AI107449   AI109427   AI114348   AI294839   AI387436   AI404239   AI404988   AI513706   BG634116   BI227883   BI354447   BI358750   BI564422   BI568808   BI572871   BI577116   BI581144   BI589533   BI607703   BI638028   BI638029   BT024263   BT030993   BT031204   BT126334   CK657197   CK658837   CK659308   CK659631   CO186991   CO191797   CO194903   CO320517   CO325140   CZ469391   CZ475250   CZ478044   CZ480346   CZ482631   CZ485230   CZ485231   EC051356   EC051851   EC053121   EC053845   EC056109   EC056200   EC056201   EC056301   EC056348   EC057438   EC078706   EC086383   EC206376   EC212602   EL884414   EL884415   EL884650   EL884651   GH941137   GH942902   GH943036   GH943318   GH943495   GH944424   GH944580   GH944880   GH946505   GH947889   GH947895   GH952628   GH953134   GH955327   GH956928   GH957901   GH957908   GH959992   GH960007   GH961142   GH962965   GH964839   GH969131   GH973008   GH973956   GH974793   GH974876   GH976501   GH977180   GH978031   GH978339   GH978728   GH979789   J02527 AAA28562   J02527 AAA28563   K01946   X00041 CAA24923   X06286 CAA29612
UniProtKB/Swiss-Prot	P00967
UniProtKB/TrEMBL	Q59DZ6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 23026
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) visceral muscle primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic hindgut   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval fat body, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval Malpighian tubules, adult heart, adult fat body, larval salivary gland, adult ovary, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body    3859.3 Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca    1430.8 Adult InseminatedFemale Spermatheca    539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body  (3859.3) Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca  (1430.8) Adult InseminatedFemale Spermatheca  (539.3) Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body  (3859.3) Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca  (1430.8) Adult InseminatedFemale Spermatheca    539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body    3859.3 Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca    1430.8 Adult InseminatedFemale Spermatheca    539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body    3859.3 Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca    1430.8 Adult InseminatedFemale Spermatheca    539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body  (3859.3) Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca  (1430.8) Adult InseminatedFemale Spermatheca  539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body  (3859.3) Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca  1430.8 Adult InseminatedFemale Spermatheca    539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    140.925 Larval Midgut    47.7 Larval Hindgut    53.1 Larval Malpighian Tubules    114.1 Larval Fat Body    3859.3 Larval Salivary Gland    106.7 Larval Trachea    79 Larval Carcass    67.25 Adult Head    294.6 Adult Eye    115.65 Adult Brain    29.8 Adult Thoracic-Abdominal Ganglion    36.7 Adult Crop    100.7 Adult Midgut    30.8 Adult Hindgut    93.3 Adult Malpighian Tubules    55.9 Adult Fat Body    386.3 Adult Salivary Gland    67.9 Adult Heart    174.7 Adult VirginFemale Spermatheca    1430.8 Adult InseminatedFemale Spermatheca    539.3 Adult Ovary    123.6 Adult Testis    16.9 Adult Male Accessory Gland    10.5 Adult Carcass    301.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    294.6   NA  Eye    115.65   NA  Brain    29.8   140.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    100.7   47.7  Midgut    no informative data   53.1  Hindgut    93.3   114.1  Malpighian Tubules    no informative data   3859.3  Fat Body    386.3   106.7  Salivary Gland    no informative data   NA  Heart    174.7   79  Trachea    NA   NA  VirginFemale Spermatheca    1430.8   NA  InseminatedFemale Spermatheca    539.3   NA  Ovary    123.6   NA  Testis    no informative data   NA  Male Accessory Gland    no informative data   67.25  Carcass    301.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    142 fat body, white prepupae    211 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering  (142) fat body, white prepupae  (211) fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering  (142) fat body, white prepupae  (211) fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    142 fat body, white prepupae    211 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    142 fat body, white prepupae    211 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering  (142) fat body, white prepupae  (211) fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering  142 fat body, white prepupae  (211) fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    6 central nervous system, larvae L3    4 central nervous system, pupae P8    1 head, virgin 1-day female    17 head, virgin 4-day female    10 head, virgin 20-day female    22 head, mated 1-day female    14 head, mated 4-day female    12 head, mated 20-day female    19 head, mated 1-day male    13 head, mated 4-day male    11 head, mated 20-day male    19 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    142 fat body, white prepupae    211 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    15 carcass, 4-day adult    12 carcass, 20-day adult    13 ovary, virgin 4-day female    7 ovary, mated 4-day female    7 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderate expression. Peak expression observed during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1    33 larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    122 larva L3 puffstage 7-9    147 white prepupae new    172 white prepupae 12hr    36 white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day    35 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1  (33) larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2  (29) larva L3 puffstage 3-6  (122) larva L3 puffstage 7-9  (147) white prepupae new  (172) white prepupae 12hr  (36) white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day  (35) adult female 05day  (32) adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1    33 larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6  (122) larva L3 puffstage 7-9  (147) white prepupae new  (172) white prepupae 12hr    36 white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day    35 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1    33 larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    122 larva L3 puffstage 7-9    147 white prepupae new    172 white prepupae 12hr    36 white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day    35 adult female 05day    32 adult female 30day    33 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1    33 larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    122 larva L3 puffstage 7-9    147 white prepupae new    172 white prepupae 12hr    36 white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day    35 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1  (33) larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2  29 larva L3 puffstage 3-6  (122) larva L3 puffstage 7-9  (147) white prepupae new  (172) white prepupae 12hr  (36) white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day  (35) adult female 05day  32 adult female 30day  (33) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1    33 larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6  122 larva L3 puffstage 7-9  (147) white prepupae new  (172) white prepupae 12hr    36 white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day    35 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    8 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    9 embryo 10-12hr    15 embryo 12-14hr    13 embryo 14-16hr    15 embryo 16-18hr    10 embryo 18-20hr    10 embryo 20-22hr    13 embryo 22-24hr    12 larva L1    33 larva L2    15 larva L3 12hr old    25 larva L3 puffstage 1-2    29 larva L3 puffstage 3-6    122 larva L3 puffstage 7-9    147 white prepupae new    172 white prepupae 12hr    36 white prepupae 24hr    20 pupae 2d postWPP    11 pupae 3d postWPP    10 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    12 adult male 30day    14 adult female 01day    35 adult female 05day    32 adult female 30day    33 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21  26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21  26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    13 embryonic 1182-4H    7 embryonic GM2    5 embryonic Kc167    11 embryonic S1    23 embryonic S3    15 leg disc CME L1    7 wing disc CME-W2    9 wing disc ML-DmD8    23 wing disc ML-DmD9    13 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    26 wing disc ML-DmD32    10 haltere disc ML-DmD17-c3    17 eye-antennal disc ML-DmD11    15 antennal disc ML-DmD20-c5    6 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    22 ovary fGS/OSS    14 ovary OSC    11 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  (28) Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    12 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    18 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    17 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    28 Ethanol 5% 3 hrs, larvae L3    8 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    20 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    19 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    6 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD26552 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000053 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31908-RA;CG31628-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 viable, with Scer\GAL4pnr-MD237 ade3GD963 Other Phenotypes Allele auxotroph | recessive ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 eye color defective ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 visible | recessive ade31 ade32 ade33 ade34 ade35 ade36 Phenotype manifest in Allele dorsal row ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 macrochaeta ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 pigment cell ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 tarsal segment ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 trichome of the posterior wing margin ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 ventral row ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 wing ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38 wing vein ade31 ade32 ade33 ade34 ade35 ade36 ade37 ade38
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of ade3 Class Mutagen Stocks Known lesion ade3d06618 P-element activity 1 -- ade3e00149 piggyBac transposase 1 -- ade3e02565 piggyBac transposase 1 -- ade3EY01643 1 -- ade3f00145 piggyBac transposase 1 -- ade3f02686 piggyBac transposase 1 -- ade3NP3593 1 -- ade31 ethyl methanesulfonate 0 Yes ade32 ethyl methanesulfonate 0 -- ade33 ethyl methanesulfonate 0 -- ade34 ethyl methanesulfonate 0 -- ade35 ethyl methanesulfonate 0 -- ade36 ethyl methanesulfonate 0 -- ade37 ethyl methanesulfonate 0 -- ade38 ethyl methanesulfonate 0 -- ade3c04641 piggyBac transposase 0 -- ade3CPTI003733 piggyBac transposase 0 Yes ade3CPTI003790 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 1 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ade3 Class Mutagen Stocks Known lesion ade3GD963 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7030 (Ryder, 2004.4.26) Df(2L)J-H (Tiong and Nash, 1990)
Not disrupted in	Df(2L)DE (Tiong and Nash, 1990) Df(2L)NL (Tiong and Nash, 1990) Df(2L)RF (Tiong and Nash, 1990)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD963} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{803.P.SVS-0}ade3CPTI003733 NA PBac{803.P.SVS-0}ade3CPTI003790 NA PBac{PB}ade3c04641 NA PBac{RB}ade3e00149 NA PBac{RB}ade3e02565 NA PBac{WH}ade3f00145 NA PBac{WH}ade3f02686 NA insertion of enhancer trap binary system P{GawB}ade3NP3593 No insertion of mobile activating element P{EPgy2}PcpEY01643 NA P{XP}ade3d06618 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR011761, InterPro:IPR013815, InterPro:IPR013816, InterPro:IPR013817, InterPro:IPR016185 (FlyBase Curators et al., 2004-) metal ion binding inferred from electronic annotation with InterPro:IPR011761 (FlyBase Curators et al., 2004-) methyltransferase activity inferred from electronic annotation with InterPro:IPR001555 (FlyBase Curators et al., 2004-) phosphoribosylamine-glycine ligase activity inferred from sequence or structural similarity with Saccharomyces ADE5,7 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Swiss-Prot Project Members, 1986.7.21) phosphoribosylformylglycinamidine cyclo-ligase activity inferred from sequence or structural similarity with Saccharomyces ADE5,7 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Swiss-Prot Project Members, 1986.7.21) phosphoribosylglycinamide formyltransferase activity inferred from electronic annotation with InterPro:IPR004607 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1986.7.21)
Biological Process
CV term Evidence References
'de novo' IMP biosynthetic process inferred from sequence or structural similarity with Saccharomyces ADE5,7 (FlyBase Genome Annotators, 2002-2003) non-traceable author statement (Swiss-Prot Project Members, 1986.7.21) purine nucleobase biosynthetic process inferred from electronic annotation with InterPro:IPR000115, InterPro:IPR020559, InterPro:IPR020560, InterPro:IPR020562 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
cytoplasm inferred from electronic annotation with InterPro:IPR004733 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
ade3 - Arpc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - Art2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - CG4806 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - CG5726 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - RpS21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - RpS27A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ade3 - Spn42Da anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ade3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60135 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000053 DroID - A comprehensive database of gene and protein interactions. • FBgn0000053 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33986
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG65X8FC Dipteran inclusive ortholog search EOG61NTV0 Insect inclusive ortholog search EOG679D2Z Arthropod inclusive ortholog search EOG6866TC Metazoa inclusive ortholog search EOG6CCGT1
Orthologs in Drosophila Species (EOG65X8FC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ade3     Drosophila simulans   Dsim\GD22518 Y   Drosophila sechellia   Dsec\GM13655 Y   Drosophila erecta   Dere\GG23557 Y   Drosophila yakuba   Dyak\GE18382 Y   Drosophila ananassae   Dana\GF14946 Y   Drosophila pseudoobscura pseudoobscura   Dpse\ade3 Y   Drosophila persimilis   Dper\GL25777 Y   Drosophila willistoni   Dwil\GK14862 Y   Drosophila virilis   Dvir\GJ13961 Y   Drosophila mojavensis   Dmoj\GI11847 Y   Drosophila grimshawi   Dgri\GH13636 Y
Orthologs in non-Drosophila Dipterans (EOG61NTV0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010640   Anopheles gambiae Malaria mosquito  Agam\AGAP009786   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009144   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009145
Orthologs in non-Dipteran Insects (EOG679D2Z)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB41233   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004793   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13102   Atta cephalotes Leafcutter ant  Acep\ACEP12264   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17627   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20142   Linepithema humile Argentine ant  Lhum\LH13519   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13228   Solenopsis invicta Red fire ant  Sinv\SINV17148   Acyrthosiphon pisum Pea aphid  Apim\ACYPI081906   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009448   Bombyx mori Silkmoth  Bmor\BGIBMGA014119   Bombyx mori Silkmoth  Bmor\BGIBMGA014118   Pediculus humanus Human body louse  Phum\PHUM292430   Tribolium castaneum Red flour beetle  Tcas\TC005772
Orthologs in non-Insect Arthropods (EOG6866TC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW017017
Orthologs in non-Arthropod Metazoa (EOG6CCGT1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F38B6.4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023531gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006361gn   Danio rerio Zebrafish  Drer\gart   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000000041   Xenopus tropicalis Western clawed frog  Xtro\gart   Gallus gallus Domestic chicken  Ggal\LOC427977   Gallus gallus Domestic chicken  Ggal\GART   Mus musculus House mouse  Mmus\Gart   Rattus norvegicus Norway rat  Rnor\Gart   Homo sapiens Human  Hsap\GART
Human Orthologs (1)
Gene OMIM HGNC Hsap\GART
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22518 Drosophila sechellia Dsec\GM13655 Drosophila erecta Dere\GG23557 Drosophila yakuba Dyak\GE18382 Drosophila ananassae Dana\GF14946 Drosophila pseudoobscura pseudoobscura Dpse\ade3 Drosophila persimilis Dper\GL25777 Drosophila willistoni Dwil\GK14862 Drosophila virilis Dvir\GJ13961 Drosophila mojavensis Dmoj\GI11847 Drosophila grimshawi Dgri\GH13636
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	15367 y1 w67c23; P{EPgy2}PcpEY01643 ade3EY01643
Harvard	- P{XP}ade3d06618 - PBac{RB}ade3e00149 - PBac{RB}ade3e02565 - PBac{WH}ade3f00145 - PBac{WH}ade3f02686
Kyoto	104552 y* w*; P{GawB}ade3NP3593 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v46293 w1118; P{GD963}v46293 v46295 w1118; P{GD963}v46295
Genomic Clones ( 2 )
	BACR13J07 BACR39J17 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 86 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP15813 LD26552
Other clones	DPiM_LD26552 IP15613 RH01206
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT30187 GH03213 GH04841 GH05139 GH08621 GH11165 GH17962 GH23875 GH24878 GH26762 GM21768 GM28603 LD05077 LP08306 LP16091 LP16983 LP17529 LP23296 RE25266 RE73847 RH02086 RH31426 RH31566 RH36390 RH37259 RH70089 SD01743 SD01744
Other clones	67686 74035 180714 CK01.26B.G12 EC32107 EC39445 EC051356 EC051851 EC053121 EC053845 EC056109 EC056200 EC056201 EC056301 EC056348 EK025145 EK195663 EK285927 IP15713 IP15913 RP004047597 RP004048002 RP004068984 RP004099943 RP004134303 RP004141410 RP004204390 RP004204442 RP004219648 RP004236124 RP004239774 RP004240723 RP004245993 RP004250649 RP004250861 RP004255103 RP004255911 RP004256347 RP004261320 RP004264378 RP004270359 RP004299561 RP004317900 RP004355094 RP004356298 RP004373071 RP004388419 RP004445553 RP004498814 RP004519366 RP004538051 RP004550201 RP004551258
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000053 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33986
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	Annotation CG8761 split into CG31628 (ade3), CG31908 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003)
Other Comments
	Nucleotide excision repair of (6-4)photoproducts has been assayed in the ade3 locus of Kc (embryonic) cell line, after exposure to UV light. The ade3 locus is transcriptionally active in Kc cells, but the repair rate is the same as for the transcriptionally inactive white gene, as well as for the transcriptionally active N gene. Repair rate is enhanced with prior exposure to photoreactivating light. No strand specificity of repair is observed. (de Cock et al., 1992) Encodes the three enzymes in the purine biosynthetic pathway, glycineamide ribotide synthetase (GARS), aminoimidazole ribotide synthetase (AIRS) and GART. (Lindsley and Zimm, 1992) Purine nucleoside auxotroph supplementable with adenine, adenosine and inosine. Recovery of ade3 progeny from crosses between ade31 and ade31/SM5 is about 1% when raised on minimal medium. Less than 3% of the normal activity purine de novo synthetic pathway enzyme, glycineamide ribotide transformylase (GART). Mutants, if viable, have a brownish eye colour and wing and bristle defects. (Lindsley and Zimm, 1992) Removal of UV-induced pyrimidine dimers is measured in genes ade3, N and w in two diploid immortalised cell lines (Kc and SL2) to investigate whether preferential repair forms part of DNA excision repair. Data supports the idea that preferential repair is not restricted to transcriptionally active sequences. (de Cock et al., 1991) The organization of the ade3 protein in Drosophila has multiple domains with GAR transformylase at the carboxyl terminus preceded by an extensive repeat. (Henikoff et al., 1983) Enzyme assays confirm that ade3 encodes GAR transformylase. (Henikoff et al., 1983)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078773 NM_001014477 RefSeq (Proteins) • NP_523497 NP_001014477 DDBJ / EMBL / GenBank • AAA28562 AAA28563 AAF52474 AAF52474.2 AAX52661 CAA24923 CAA29612 CZ469391 CZ475250 CZ478044 CZ480346 CZ482631 CZ485230 CZ485231 J02527 K01946 X00041 X06286 Entrez Gene - A searchable database of RefSeq genes. • 33986 UniProtKB/Swiss-Prot • P00967 UniProtKB/TrEMBL • Q59DZ6
Other Crossreferences
	EPD - Eukarytoic Promoter Database, an annotated collection of POL II promoters • 23026 InterPro domains - A database of protein families, domains, and functional sites • ATP-grasp fold, subdomain 1 (IPR013815) ATP-grasp fold, subdomain 2 (IPR013816) Phosphoribosylglycinamide synthetase (IPR000115) AIR synthase related protein, N-terminal domain (IPR000728) Phosphoribosylglycinamide formyltransferase, active site (IPR001555) Formyl transferase, N-terminal (IPR002376) Phosphoribosylglycinamide formyltransferase (IPR004607) Phosphoribosylformylglycinamidine cyclo-ligase (IPR004733) AIR synthase-related protein, C-terminal domain (IPR010918) Rudiment single hybrid motif (IPR011054) ATP-grasp fold (IPR011761) Pre-ATP-grasp domain (IPR016185) PurM, N-terminal-like (IPR016188) Phosphoribosylglycinamide synthetase, conserved site (IPR020559) Phosphoribosylglycinamide synthetase, C-domain (IPR020560) Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain (IPR020561) Phosphoribosylglycinamide synthetase, N-terminal (IPR020562) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD26552
Linkouts
	BioGRID - A database of protein and genetic interactions • 60135 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000053 DroID - A comprehensive database of gene and protein interactions. • FBgn0000053 DRSC - Results from RNAi screens. • FBgn0000053 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000053 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31908-RA;CG31628-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000053 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33986 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33986 modMine - Data generated by the modENCODE project. • FBgn0000053
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	143062_at (Kristensen et al., 2005) ade3 (FlyBase Curators, 2008, de Wit et al., 2005, Curtis et al., 2007, Ellis and Carney, 2010, Taraszka et al., 2005, Sun et al., 2012) Ade3 (Gruenewald et al., 2009) ADE8 (Henikoff et al., 1983) CG8761   CG31628 (Ellis and Carney, 2010) D (Tiong and Nash, 1990) Gart (Gibson et al., 2005, Civetta and Singh, 1998, Clark et al., 1998, van der Helm et al., 1997, de Cock et al., 1992, de Cock et al., 1992, Moriyama and Gojobori, 1992, de Cock et al., 1991, ) GART (van Steensel et al., 2001, Vernick and McCutchan, 1998, Davidson and Peterson, 1997) gart (Eickbush et al., 1995) Gart/ade3 (Comeron and Kreitman, 1998) l(2)27Da
Name Synonym	adenosine 3 (Scherzer et al., 2003, Beckstead et al., 2007, Sun et al., 2012) Complementation group D (Tiong and Nash, 1990)
Secondary FlyBase IDs
	FBgn0051628 FBgn0063628
References ( 66 )
Generate a list of	All references relating to this gene ( 66 )
List References by type	Research paper  ( 39 ) Supplementary material  ( 2 ) Review  ( 4 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 2 ) FlyBase analysis  ( 3 ) Letter  ( 1 ) Book  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 3 )
Recent research papers ( 2 )
	Landis et al., 2012, Aging 4(11): 768--789 Gene expression changes in response to aging compared to heat stress, oxidative stress and ionizing radiation in Drosophila melanogaster. [FBrf0220226] Sun et al., 2012, Aging Cell 11(5): 783--793 Nutrient-dependent requirement for SOD1 in lifespan extension by protein restriction in Drosophila melanogaster. [FBrf0219634] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...