A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\Adf1

General Information
SymbolDmel\Adf1SpeciesD. melanogaster
NameAdh transcription factor 1Annotation symbolCG15845
Feature typeprotein_coding_geneFlyBase IDFBgn0000054
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)2RRecombination map
Cytogenetic map 42C3-42C3 Sequence location2R:2,549,774..2,555,592 [+]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene Adh transcription factor 1 is referred to in FlyBase by the symbol Adf1 (CG15845, FBgn0000054). It has the cytological map location 42C3. Its sequence location is 2R:2549774..2555592. Its molecular function is described as: general RNA polymerase II transcription factor activity; RNA polymerase II transcription factor activity; DNA binding. It is involved in the biological processes: memory; synaptogenesis; olfactory learning; long-term memory; positive regulation of transcription; regulation of transcription from RNA polymerase II promoter; larval locomotory behavior; learning and/or memory; locomotion; dendrite morphogenesis. 45 alleles are reported. The phenotypes of these alleles are annotated with: bouton; scutellar bristle; dorsocentral bristle; anterior fascicle. It has 3 annotated transcripts and 3 annotated polypeptides.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
42C3-42C3  
Limits computationally determined from genome sequence between P{lacW}geminink14019&P{PZ}Adf101349 and P{lacW}ublk03203&P{PZ}CG328709967  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
42C1-42C2
(determined by in situ hybridisation)  
(Abdelilah-Seyfried et al., 2000)
42C1-42C2
(determined by in situ hybridisation)  
(Spradling et al., 1999)
42C1-42C4
42C3--4  
(BDGP Project Members, 1994-1999)
42C2-42C7
(determined by in situ hybridisation)  
(England et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: cn20- Adf1+
(DeZazzo et al., 2000)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\Adf1 for information on other features
detailed view FBtr0086110 FBtr0086146 FBtr0086112 FBtr0086113 FBtr0086111 FBtr0086114 FBtr0299799 FBtr0299800 FBtr0086115 FBtr0086116 FBpp0085479 FBpp0089262 FBpp0085446 FBpp0085447 FBpp0085448 FBpp0289077 FBpp0085450 FBpp0289078 FBpp0085449 FBti0020900 FBti0070968 FBti0071075 FBti0005259 FBti0007701 FBti0028608 FBti0034603 FBti0071025 FBti0005187 FBti0071194 FBti0059421 FBti0036996 FBti0016957 FBti0099956 FBti0025848 FBti0056589 FBti0037214 FBti0010883 FBti0006397 FBti0045463 FBti0066387 FBti0046746 FBti0046886 FBti0024560 FBti0029967
Comments on Gene Model
Transposon inserted in intron
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
Adf1-RA
FBtr0086111
 NM_165479
  1666
  253
Adf1-RB
FBtr0086112
 NM_165480
  1798
  253
Adf1-RC
FBtr0086113
 NM_206028
  1678
  262
Additional Transcript Data & Comments
Reported size (kB)
1.9 (northern blot)
(England et al., 1992)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
Adf1-PA  
FBpp0085446  
29.2  
253  
8.74  
  NP_724481
  AAF57369
Adf1-PB  
FBpp0085447  
29.2  
253  
8.74  
  NP_724482
  AAF57370
Adf1-PC  
FBpp0089262  
30.2  
262  
8.44  
  NP_995750
  AAS64778
Additional Polypeptide Data & Comments
Reported size (kD)
253 (aa); 29 (kD)
(England et al., 1992)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
BESS motif (IPR004210)
MADF (IPR006578)
TRANSFAC - Eukaryotic transcription factors, their genomic binding sites, and DNA-binding profiles
•
  • R04245
  • T00008
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA390609
 
AA441231
 
AE003789
AAF57369
 
AE003789
AAF57370
 
AE003789
AAS64778
 
AI108659
 
AI517289
 
AQ024962
 
AQ025586
 
AQ025622
 
AQ072936
 
AW942083
 
AY069387
AAL39532
 
BI213463
 
BI227827
 
BI227837
 
BI357568
 
BI365033
 
BI614852
 
BP548747
 
BP548748
 
CK661393
 
CO316176
 
CO317674
 
CO318507
 
CO321838
 
CO323376
 
CZ470134
 
CZ471168
 
CZ476144
 
EC070958
 
EC074608
 
EC075082
 
EC082769
 
EC088303
 
EC197515
 
EC204169
 
EC227035
 
EC238841
 
EC240983
 
EC253297
 
M37787
AAA28325
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Spradling et al., 1999, BDGP Project Members, 2000-)
    (BDGP Project Members, 1994-1999)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       embryonic stage | >4 hr
       
      (England et al., 1992)
      Marker for
        Subcellular Localization
        CV Term
        nucleus
        (England et al., 1992)
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for Adf1
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for Adf1
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        lethal
        lethal | recessive
        lethal | embryonic stage | recessive
        lethal, with Scer\GAL4C321c
        lethal, with Scer\GAL4MZ1580
        lethal, with Scer\GAL4Tab2-201Y
        lethal, with Scer\GAL4238Y
        lethal, with Scer\GAL4OK348
        lethal, with Scer\GAL4OK107
        lethal, with Scer\GAL4c747
        semi-lethal
        semi-lethal | recessive
        Other Phenotypes
        Allele
        wild-type
        visible, with Scer\GAL4ptc-559.1
        memory defective
        memory defective | recessive
        neuroanatomy defective
        neuroanatomy defective | larval stage, with Scer\GAL4elav-C155
        Phenotype manifest in
        Allele
        dorsal multidendritic neuron ddaE & dendrite
        abdominal dorsal multidendritic neuron ddaE & dendrite
        scutellar bristle | ectopic, with Scer\GAL4ptc-559.1
        dorsocentral bristle | ectopic, with Scer\GAL4ptc-559.1
        anterior fascicle, with Scer\GAL4elav-C155
        anterior fascicle & synapse, with Scer\GAL4elav-C155
        bouton | ectopic
        bouton
        hide Classical Alleles ( 14 )
        For All Classical Alleles Show

        Allele of Adf1ClassMutagenStocksKnown lesion
        Adf1013491 --
        Adf1040650 Yes
        Adf1CB022760 Yes
        Adf1EP21471 --
        Adf1EP8151 Yes
        Adf1c056181 --
        Adf1c070180 --
        Adf1d082331 --
        Adf1k006201 --
        Adf1k099191 --
        Adf1le550 Yes
        Adf1le600 Yes
        Adf1nalhypomorph0 Yes
        Adf1ve480 Yes
        hide Alleles Carried on Transgenic Constructs ( 31 )
        For All Alleles Carried on Transgenic Constructs Show

        Allele of Adf1ClassMutagenStocksKnown lesion
        Adf13A131.Act5C0 Yes
        Adf14A102.Act5C0 Yes
        Adf14A117.Act5C0 Yes
        Adf15A144.Act5C0 Yes
        Adf15A158.Act5C0 Yes
        Adf15A172.Act5C0 Yes
        Adf15A186.Act5C0 Yes
        Adf15A200.Act5C0 Yes
        Adf15A214.Act5C0 Yes
        Adf15A228.Act5C0 Yes
        Adf15A245.Act5C0 Yes
        Adf15A88.Act5C0 Yes
        Adf1Act5C.PC0 Yes
        Adf1GD13581 Yes
        Adf1N130.Act5C0 Yes
        Adf1N228.Act5C0 Yes
        Adf1Scer\UAS.cDa0 Yes
        Adf1hs.PD0 Yes
        Adf1Δ102-120.Act5C0 Yes
        Adf1Δ117-133.Act5C0 Yes
        Adf1Δ131-148.Act5C0 Yes
        Adf1Δ144-162.Act5C0 Yes
        Adf1Δ158-176.Act5C0 Yes
        Adf1Δ172-190.Act5C0 Yes
        Adf1Δ186-204.Act5C0 Yes
        Adf1Δ200-218.Act5C0 Yes
        Adf1Δ214-232.Act5C0 Yes
        Adf1Δ60-78.Act5C0 Yes
        Adf1Δ88-105.Act5C0 Yes
        Adf1ΔN12.Act5C0 Yes
        Adf1ΔN5.Act5C0 Yes
        hide Aneuploid Aberrations
        Useful deficiency
         
        Disrupted in
        (DeZazzo et al., 2000, BDGP Project Members, 1994-1999)
        (BDGP Project Members, 1994-1999)
        (DeZazzo et al., 2000)
        (BDGP Project Members, 1994-1999)
        (BDGP Project Members, 1994-1999)
        Not disrupted in
        (BDGP Project Members, 1994-1999)
        (DeZazzo et al., 2000)
        (DeZazzo et al., 2000)
        (BDGP Project Members, 1994-1999)
        (BDGP Project Members, 1994-1999)
        (BDGP Project Members, 1994-1999)
        (BDGP Project Members, 1994-1999)
        hide Transgenic Constructs & Insertions
        Transgenic Constructs
        Type of construct
        Name
        Expression data
        heat-shock construct
        P{hs-Adf1.D}
        NA
        UAS construct
        P{GD1358}
        NA
        P{UAS-Adf1.D}
        NA
        Insertions
        Type of insertions
        Name
        Expression data
        insertion of mobile activating element
        P{EP}Adf1EP2147
        NA
        P{EP}Adf1EP815
        NA
        P{XP}Adf1d08233
        NA
        miscellaneous insertions
        PBac{PB}Adf1c05618
        NA
        PBac{PB}Adf1c07018
        NA
        P{PTT-GB}Adf1CB02276
        NA
        insertion of enhancer trap
        P{PZ}Adf101349
        Yes
        P{PZ}Dpit4704065
        Yes
        P{lacW}Adf1k00620
        No
        P{lacW}Adf1k09919
        No
        P{lacW}Adf1nal
        No
        P{lacW}l(2)SH1631SH1631
        No
        hide Related Comments
        Please look at the allele reports for the complete phenotype data
        Adf1 mutants have defects in larval locomotion.
        (Parrish et al., 2006)
        dsRNA has been made from templates generated with primers directed against this gene. RNAi of Adf1 results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
        (Parrish et al., 2006)
        hide Gene Ontology: Function, Process & Cellular Component ( 14 )
        hide Molecular Function
        CV term
        Evidence
        References
        DNA binding
        inferred from electronic annotation with InterPro:IPR004210
        (FlyBase Curators et al., 2004-)
        RNA polymerase II transcription factor activity
        non-traceable author statement
        (Swiss-Prot Project Members, 1991.8.1)
        general RNA polymerase II transcription factor activity
        inferred from sequence or structural similarity
        (Spradling et al., 1999)
        hide Biological Process
        CV term
        Evidence
        References
        dendrite morphogenesis
        inferred from mutant phenotype
        (Parrish et al., 2006)
        larval locomotory behavior
        non-traceable author statement
        (Sokolowski, 2001)
        learning and/or memory
        non-traceable author statement
        (Sokolowski, 2001)
        locomotion
        inferred from mutant phenotype
        (Parrish et al., 2006)
        long-term memory
        traceable author statement
        (Burrell and Sahley, 2001)
        memory
        inferred from mutant phenotype
        (DeZazzo et al., 2000)
        olfactory learning
        non-traceable author statement
        (Roman and Davis, 2001)
        positive regulation of transcription
        traceable author statement
        (Burrell and Sahley, 2001)
        regulation of transcription from RNA polymerase II promoter
        non-traceable author statement
        (Swiss-Prot Project Members, 1991.8.1)
        synaptogenesis
        inferred from mutant phenotype
        (DeZazzo et al., 2000)
        hide Cellular Component
        CV term
        Evidence
        References
        nucleus
        inferred from sequence or structural similarity
        (Spradling et al., 1999)
        inferred from direct assay
        (DeZazzo et al., 2000)
        non-traceable author statement
        (Swiss-Prot Project Members, 1991.8.1)
        hide Sequence Ontology: Class of Gene
        protein_coding_gene
         
        nuclear_gene
         
        hide Interactions & Pathways
        hide Summary of Genetic Interactions
        Interacts with
        Please look at the allele data for full details of the genetic interactions
        Adf1 allele
        Gene
        References
        hide External Data
        Linkouts
        BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
        •