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Gene Dmel\Adf1

General Information
Symbol	Dmel\Adf1	Species	D. melanogaster
Name	Adh transcription factor 1	Annotation symbol	CG15845
Feature type	protein_coding_gene	FlyBase ID	FBgn0000054
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	Adf-1, nal, Adf 1, l(2)01349
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42C3-42C3	Sequence location	2R:2,549,770..2,555,590 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Adh transcription factor 1 is referred to in FlyBase by the symbol Dmel\Adf1 (CG15845, FBgn0000054). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription. There is experimental evidence that it is involved in the biological process: synapse assembly; positive regulation of transcription from RNA polymerase II promoter; locomotion; dendrite morphogenesis; memory. 56 alleles are reported. The phenotypes of these alleles are annotated with: intersegmental nerve; bouton. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: BESS motif; MADF domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult thoracico-abdominal ganglion, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1636020_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Adf1. Gene sequence location is 2R:2549770..2555590.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42C3-42C3   Limits computationally determined from genome sequence between P{lacW}geminink14019&P{PZ}Adf101349 and P{lacW}ublk03203&P{PZ}CG328709967
Experimentally Determined Cytological Location
Cytogenetic map Notes References 42C1-42C2   (determined by in situ hybridisation)   (Abdelilah-Seyfried et al., 2000) 42C1-42C2   (determined by in situ hybridisation)   (Spradling et al., 1999) 42C1-42C4   42C3--4   (BDGP Project Members, 1994-1999) 42C2-42C7   (determined by in situ hybridisation)   (England et al., 1992)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Adf1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.48 Gene model reviewed during 5.44 gene_with_stop_codon_read_through ; SO:0000697 Stop-codon suppression (UAA) postulated; FBrf0216884 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Adf1-RA FBtr0086111  NM_165479   1668   253 Adf1-RB FBtr0086112  NM_165480   1795   253 Adf1-RC FBtr0086113  NM_206028   1675   262 Adf1-RE FBtr0330252  NM_001273801   1668   274
Additional Transcript Data & Comments
Reported size (kB)	1.9 (northern blot) (England et al., 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Adf1-PA   FBpp0085446   29.2   253   8.74     NP_724481   AAF57369 Adf1-PB   FBpp0085447   29.2   253   8.74     NP_724482   AAF57370 Adf1-PC   FBpp0089262   30.2   262   8.44     NP_995750   AAS64778 Adf1-PE   FBpp0303284   31.5   274   8.58     NP_001260730   AGB93263
Additional Polypeptide Data & Comments
Reported size (kDa)	253 (aa); 29 (kD) (England et al., 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • BESS motif (IPR004210) MADF domain (IPR006578)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA390609   AA441231   AE013599 AAF57369   AE013599 AAF57370   AE013599 AAS64778   AE013599 AGB93263   AI108659   AI133997   AI517289   AQ024962   AQ025586   AQ025622   AQ072936   AW942083   AY069387 AAL39532   BG639861   BI213463   BI227827   BI227837   BI357568   BI365033   BP548747   BP548748   BT088961   CK661393   CO189178   CO270744   CO276084   CO283537   CO290680   CO292299   CO316176   CO317674   CO318507   CO321838   CO323376   CO331131   CZ470134   CZ471168   CZ476144   EC070958   EC074608   EC075082   EC082769   EC088303   EC197515   EC204169   EC227035   EC238841   EC240983   EC253297   EV575549   M37787 AAA28325
UniProtKB/Swiss-Prot	P05552
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | intense & ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | intense & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) inclusive hindgut primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) plasmatocyte primordium   (Fisher et al., 2012) Comment:reported as plasmatocytes anlage posterior midgut primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) embryonic/larval salivary gland body   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10 -- 17   organism | ubiquitous   (England et al., 1992) Comment:reference states >4 hr AEL
Additional Descriptive Data
	Adf1 is expressed at nearly constant levels throughout the embryo from around 4 or 5 hours after fertilizaton onward. Expression is seen in the nuclei of nearly all cells with the exception of the pole cells. (England et al., 1992)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (DeZazzo et al., 2000, Heberlein et al., 1985, England et al., 1992)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult thoracico-abdominal ganglion, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain    629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary    603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (633.5) Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain  (629.9) Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary  (603.5) Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain    629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary    603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain    629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary    603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain    629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary    603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain  629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary  603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain    629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary    603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    633.5 Larval Midgut    152.9 Larval Hindgut    232.5 Larval Malpighian Tubules    249.5 Larval Fat Body    187.4 Larval Salivary Gland    456.9 Larval Trachea    415.85 Larval Carcass    184.75 Adult Head    352.8 Adult Eye    381.5 Adult Brain    629.9 Adult Thoracic-Abdominal Ganglion    399.9 Adult Crop    326.5 Adult Midgut    198.2 Adult Hindgut    247.9 Adult Malpighian Tubules    228.5 Adult Fat Body    156.1 Adult Salivary Gland    450.2 Adult Heart    167.325 Adult VirginFemale Spermatheca    238.7 Adult InseminatedFemale Spermatheca    259.3 Adult Ovary    603.5 Adult Testis    135.9 Adult Male Accessory Gland    332.7 Adult Carcass    185.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    352.8   NA  Eye    381.5   NA  Brain    629.9   633.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    399.9   NA  Crop    326.5   152.9  Midgut    198.2   232.5  Hindgut    247.9   249.5  Malpighian Tubules    228.5   187.4  Fat Body    156.1   456.9  Salivary Gland    450.2   NA  Heart    167.325   415.85  Trachea    NA   NA  VirginFemale Spermatheca    238.7   NA  InseminatedFemale Spermatheca    259.3   NA  Ovary    603.5   NA  Testis    135.9   NA  Male Accessory Gland    332.7   184.75  Carcass    185.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    50 central nervous system, larvae L3    54 central nervous system, pupae P8    31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    30 head, mated 4-day male    20 head, mated 20-day male    26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering    36 carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    52 ovary, mated 4-day female    43 testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (50) central nervous system, larvae L3  (54) central nervous system, pupae P8  (31) head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male  (30) head, mated 4-day male    20 head, mated 20-day male  26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering  (36) carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female  (52) ovary, mated 4-day female  (43) testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    50 central nervous system, larvae L3    54 central nervous system, pupae P8    31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    30 head, mated 4-day male    20 head, mated 20-day male    26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering    36 carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    52 ovary, mated 4-day female    43 testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    50 central nervous system, larvae L3    54 central nervous system, pupae P8    31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    30 head, mated 4-day male    20 head, mated 20-day male    26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering    36 carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    52 ovary, mated 4-day female    43 testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    50 central nervous system, larvae L3    54 central nervous system, pupae P8    31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    30 head, mated 4-day male    20 head, mated 20-day male    26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering    36 carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    52 ovary, mated 4-day female    43 testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (50) central nervous system, larvae L3  (54) central nervous system, pupae P8  31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male  30 head, mated 4-day male    20 head, mated 20-day male  26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering  (36) carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female  (52) ovary, mated 4-day female  (43) testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    50 central nervous system, larvae L3    54 central nervous system, pupae P8    31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    30 head, mated 4-day male    20 head, mated 20-day male    26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering    36 carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    52 ovary, mated 4-day female    43 testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    50 central nervous system, larvae L3    54 central nervous system, pupae P8    31 head, virgin 1-day female    13 head, virgin 4-day female    16 head, virgin 20-day female    13 head, mated 1-day female    16 head, mated 4-day female    12 head, mated 20-day female    12 head, mated 1-day male    30 head, mated 4-day male    20 head, mated 20-day male    26 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    20 digestive system, 1-day adult    7 digestive system, 4-day adult    9 digestive system, 20-day adult    10 fat body, larvae L3 wandering    4 fat body, white prepupae    15 fat body, pupae P8    21 carcass, larvae L3 wandering    36 carcass, 1-day adult    8 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    52 ovary, mated 4-day female    43 testis, mated 4-day male    12 accessory gland, mated 4-day male    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    48 embryo 02-04hr    59 embryo 04-06hr    78 embryo 06-08hr    96 embryo 08-10hr    64 embryo 10-12hr    59 embryo 12-14hr    51 embryo 14-16hr    44 embryo 16-18hr    31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    36 white prepupae 12hr    31 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day    32 adult female 30day    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (48) embryo 02-04hr  (59) embryo 04-06hr  (78) embryo 06-08hr  (96) embryo 08-10hr  (64) embryo 10-12hr  (59) embryo 12-14hr  (51) embryo 14-16hr  (44) embryo 16-18hr  (31) embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6  (27) larva L3 puffstage 7-9  (33) white prepupae new  (36) white prepupae 12hr  (31) white prepupae 24hr  (45) pupae 2d postWPP  (37) pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day  (32) adult female 30day  (30) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    48 embryo 02-04hr    59 embryo 04-06hr    78 embryo 06-08hr    96 embryo 08-10hr    64 embryo 10-12hr    59 embryo 12-14hr    51 embryo 14-16hr    44 embryo 16-18hr    31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    36 white prepupae 12hr    31 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day    32 adult female 30day    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    48 embryo 02-04hr    59 embryo 04-06hr    78 embryo 06-08hr    96 embryo 08-10hr    64 embryo 10-12hr    59 embryo 12-14hr    51 embryo 14-16hr    44 embryo 16-18hr    31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    36 white prepupae 12hr    31 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day    32 adult female 30day    30 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    48 embryo 02-04hr    59 embryo 04-06hr    78 embryo 06-08hr    96 embryo 08-10hr    64 embryo 10-12hr    59 embryo 12-14hr    51 embryo 14-16hr    44 embryo 16-18hr    31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    36 white prepupae 12hr    31 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day    32 adult female 30day    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (48) embryo 02-04hr  (59) embryo 04-06hr  (78) embryo 06-08hr  (96) embryo 08-10hr  (64) embryo 10-12hr  (59) embryo 12-14hr  (51) embryo 14-16hr  (44) embryo 16-18hr  31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6  27 larva L3 puffstage 7-9  (33) white prepupae new  (36) white prepupae 12hr  31 white prepupae 24hr  (45) pupae 2d postWPP  (37) pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day  32 adult female 30day  30 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    48 embryo 02-04hr    59 embryo 04-06hr    78 embryo 06-08hr    96 embryo 08-10hr    64 embryo 10-12hr    59 embryo 12-14hr    51 embryo 14-16hr    44 embryo 16-18hr    31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    36 white prepupae 12hr    31 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day    32 adult female 30day    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    48 embryo 02-04hr    59 embryo 04-06hr    78 embryo 06-08hr    96 embryo 08-10hr    64 embryo 10-12hr    59 embryo 12-14hr    51 embryo 14-16hr    44 embryo 16-18hr    31 embryo 18-20hr    24 embryo 20-22hr    14 embryo 22-24hr    16 larva L1    15 larva L2    17 larva L3 12hr old    12 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    33 white prepupae new    36 white prepupae 12hr    31 white prepupae 24hr    45 pupae 2d postWPP    37 pupae 3d postWPP    16 pupae 4d postWPP    14 adult male 01day    13 adult male 05day    16 adult male 30day    16 adult female 01day    23 adult female 05day    32 adult female 30day    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    23 embryonic 1182-4H    33 embryonic GM2    25 embryonic Kc167    14 embryonic S1    43 embryonic S3    25 leg disc CME L1    29 wing disc CME-W2    17 wing disc ML-DmD8    33 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    34 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS    70 ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (40) Schneider line 2 Sg4    23 embryonic 1182-4H  (33) embryonic GM2    25 embryonic Kc167    14 embryonic S1  (43) embryonic S3    25 leg disc CME L1  (29) wing disc CME-W2    17 wing disc ML-DmD8  (33) wing disc ML-DmD9  (37) wing disc ML-DmD16-c3  (34) wing disc ML-DmD21  (34) wing disc ML-DmD32  (28) haltere disc ML-DmD17-c3  (31) eye-antennal disc ML-DmD11  (30) antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1  26 CNS ML-DmBG1-c1  (29) CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS  (70) ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    23 embryonic 1182-4H    33 embryonic GM2    25 embryonic Kc167    14 embryonic S1    43 embryonic S3    25 leg disc CME L1    29 wing disc CME-W2    17 wing disc ML-DmD8    33 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    34 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS    70 ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    23 embryonic 1182-4H    33 embryonic GM2    25 embryonic Kc167    14 embryonic S1    43 embryonic S3    25 leg disc CME L1    29 wing disc CME-W2    17 wing disc ML-DmD8    33 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    34 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS    70 ovary OSC    24 ovary OSS    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    23 embryonic 1182-4H    33 embryonic GM2    25 embryonic Kc167    14 embryonic S1    43 embryonic S3    25 leg disc CME L1    29 wing disc CME-W2    17 wing disc ML-DmD8    33 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    34 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS    70 ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (40) Schneider line 2 Sg4    23 embryonic 1182-4H  (33) embryonic GM2    25 embryonic Kc167    14 embryonic S1  (43) embryonic S3    25 leg disc CME L1  29 wing disc CME-W2    17 wing disc ML-DmD8  (33) wing disc ML-DmD9  (37) wing disc ML-DmD16-c3  (34) wing disc ML-DmD21  (34) wing disc ML-DmD32  28 haltere disc ML-DmD17-c3  31 eye-antennal disc ML-DmD11  30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1  26 CNS ML-DmBG1-c1  29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS  (70) ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    23 embryonic 1182-4H    33 embryonic GM2    25 embryonic Kc167    14 embryonic S1    43 embryonic S3    25 leg disc CME L1    29 wing disc CME-W2    17 wing disc ML-DmD8    33 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    34 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS    70 ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    40 Schneider line 2 Sg4    23 embryonic 1182-4H    33 embryonic GM2    25 embryonic Kc167    14 embryonic S1    43 embryonic S3    25 leg disc CME L1    29 wing disc CME-W2    17 wing disc ML-DmD8    33 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    34 wing disc ML-DmD32    28 haltere disc ML-DmD17-c3    31 eye-antennal disc ML-DmD11    30 antennal disc ML-DmD20-c5    19 mixed discs ML-DmD4-c1    26 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    25 ovary fGS/OSS    70 ovary OSC    24 ovary OSS    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    27 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  (29) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (33) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (35) Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult  (27) Paraquat 10 mM 48 hrs, 4-day adult  26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    27 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    27 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    27 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (33) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (35) Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult  27 Paraquat 10 mM 48 hrs, 4-day adult  26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    27 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    23 cold shock, 4-day adult    19 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    21 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    13 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    35 Caffeine 25 mg/ml 48 hrs, 4-day adult    13 Paraquat 5 mM 48 hrs, 4-day adult    27 Paraquat 10 mM 48 hrs, 4-day adult    26 Rotenone 2 μg 12 hrs, larvae L3    15 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD09689 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000054 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG15845-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Adf1 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Adf1CB02276 lethal, with Scer\GAL4238Y Adf1Scer\UAS.cDa lethal, with Scer\GAL4C321c Adf1Scer\UAS.cDa lethal, with Scer\GAL4c747 Adf1Scer\UAS.cDa lethal, with Scer\GAL4ey-OK107 Adf1Scer\UAS.cDa lethal, with Scer\GAL4MZ1580 Adf1Scer\UAS.cDa lethal, with Scer\GAL4OK348 Adf1Scer\UAS.cDa lethal, with Scer\GAL4Tab2-201Y Adf1Scer\UAS.cDa lethal | embryonic stage | recessive Adf1le60 lethal | recessive Adf101349 Adf104065 Adf1k00620 Adf1k09919 partially lethal - majority die Adf104065 partially lethal - majority die | recessive Adf1le60 viable, with Scer\GAL4pnr-MD237 Adf1GD1358 Other Phenotypes Allele memory defective Adf1hs.PD memory defective | recessive Adf1le55 Adf1le60 Adf1nal Adf1ve48 neuroanatomy defective Adf101349 Adf1hs.PD Adf1nal neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 Adf1EP815 visible, with Scer\GAL4ptc-559.1 Adf1EP815 wild-type Adf1hs.PD Phenotype manifest in Allele abdominal dorsal multidendritic neuron ddaE & dendrite Adf101349 anterior fascicle & synapse, with Scer\GAL4elav-C155 Adf1EP815 bouton Adf1nal bouton | ectopic Adf1hs.PD dorsal multidendritic neuron ddaE & dendrite Adf101349 dorsocentral bristle | ectopic, with Scer\GAL4ptc-559.1 Adf1EP815 intersegmental nerve, with Scer\GAL4elav-C155 Adf1EP815 scutellar bristle | ectopic, with Scer\GAL4ptc-559.1 Adf1EP815
Classical Alleles ( 21 )
For All Classical Alleles Show
Allele of Adf1 Class Mutagen Stocks Known lesion Adf101349 P-element activity 1 -- Adf15-HA-2159 1 -- Adf15-HA-2715 1 -- Adf1c05618 piggyBac transposase 1 -- Adf1CB-5490-3 1 -- Adf1d08233 P-element activity 1 -- Adf1k00620 P-element activity 1 -- Adf1k09919 P-element activity 1 -- Adf1NP2090 1 -- Adf1NP6402 1 -- Adf1NP7083 1 -- Adf104065 P-element activity 0 Yes Adf1c07018 piggyBac transposase 0 -- Adf1CB02276 P-element activity 0 Yes Adf1EP2147 P-element activity 0 -- Adf1EP815 P-element activity 0 Yes Adf1le55 P-element activity 0 Yes Adf1le60 P-element activity 0 Yes Adf1nal hypomorphic allele - genetic evidence P-element activity 0 Yes Adf1SH0340 0 -- Adf1ve48 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 35 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Adf1 Class Mutagen Stocks Known lesion Adf1GD1358 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Adf1HM04004 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Adf1JF03095 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Adf1KK111012 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Adf13A131.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf14A102.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf14A117.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A144.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A158.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A172.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A186.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A200.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A214.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A228.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A245.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf15A88.Act5C in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes Adf1Act5C.PC in vitro construct - regulatory fusion 0 Yes Adf1hs.PD in vitro construct - regulatory fusion 0 Yes Adf1N130.Act5C in vitro construct - deletion 0 Yes Adf1N228.Act5C in vitro construct - deletion 0 Yes Adf1NIG.15845R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Adf1Scer\UAS.cDa in vitro construct - regulatory fusion 0 Yes Adf1Δ102-120.Act5C in vitro construct - deletion 0 Yes Adf1Δ117-133.Act5C in vitro construct - deletion 0 Yes Adf1Δ131-148.Act5C in vitro construct - deletion 0 Yes Adf1Δ144-162.Act5C in vitro construct - deletion 0 Yes Adf1Δ158-176.Act5C in vitro construct - deletion 0 Yes Adf1Δ172-190.Act5C in vitro construct - deletion 0 Yes Adf1Δ186-204.Act5C in vitro construct - deletion 0 Yes Adf1Δ200-218.Act5C in vitro construct - deletion 0 Yes Adf1Δ214-232.Act5C in vitro construct - deletion 0 Yes Adf1Δ60-78.Act5C in vitro construct - deletion 0 Yes Adf1Δ88-105.Act5C in vitro construct - deletion 0 Yes Adf1ΔN12.Act5C in vitro construct - deletion 0 Yes Adf1ΔN5.Act5C in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)42 (DeZazzo et al., 2000, BDGP Project Members, 1994-1999) Df(2R)cn88b (BDGP Project Members, 1994-1999) Df(2R)hfl-37 (Rendina et al., 2010) Df(2R)nap2 (BDGP Project Members, 1994-1999) Df(2R)nap12 (DeZazzo et al., 2000) Df(2R)nap16 (BDGP Project Members, 1994-1999)
Not disrupted in	Df(2R)42 (BDGP Project Members, 1994-1999) Df(2R)cn87e (DeZazzo et al., 2000) Df(2R)cn88b (DeZazzo et al., 2000) Df(2R)nap5 (BDGP Project Members, 1994-1999) Df(2R)pk78s (BDGP Project Members, 1994-1999) In(2LR)TE35B-4LTE35B-14R (BDGP Project Members, 1994-1999) In(2LR)TE35B-14LTE35B-4R (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1358} NA P{KK111012} NA P{NIG.15845R} NA P{TRiP.HM04004} NA P{TRiP.JF03095} NA P{UAS-Adf1.D} NA heat-shock construct P{hs-Adf1.D} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Adf1k00620 No P{lacW}Adf1k09919 No P{lacW}Adf1nal No P{lacW}Adf1SH0340 No P{lacW}l(2)SH1631SH1631 No P{PZ}Adf101349 No P{PZ}Dpit4704065 No insertion of mobile activating element P{EP}Adf1EP815 NA P{EP}Adf1EP2147 NA P{XP}Adf1d08233 NA miscellaneous insertions P{PTT-GB}Adf1CB02276 NA P{RS3}Adf1CB-5490-3 NA P{RS5}Adf15-HA-2159 NA P{RS5}Adf15-HA-2715 NA PBac{PB}Adf1c05618 NA PBac{PB}Adf1c07018 NA insertion of enhancer trap binary system P{GawB}Adf1NP2090 No P{GawB}Adf1NP6402 No P{GawB}Adf1NP7083 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription inferred from direct assay (Heberlein et al., 1985)
Biological Process
CV term Evidence References
dendrite morphogenesis inferred from mutant phenotype (Parrish et al., 2006) locomotion inferred from mutant phenotype (Parrish et al., 2006) memory inferred from mutant phenotype (DeZazzo et al., 2000) positive regulation of transcription from RNA polymerase II promoter inferred from direct assay (Heberlein et al., 1985) synapse assembly inferred from mutant phenotype (DeZazzo et al., 2000)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (DeZazzo et al., 2000, Heberlein et al., 1985, England et al., 1992)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR004210 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
larval locomotory behavior non-traceable author statement (Sokolowski, 2001) learning or memory non-traceable author statement (Sokolowski, 2001) long-term memory traceable author statement (Burrell and Sahley, 2001) olfactory learning non-traceable author statement (Roman and Davis, 2001) positive regulation of transcription, DNA-dependent traceable author statement (Burrell and Sahley, 2001) regulation of transcription from RNA polymerase II promoter non-traceable author statement (Swiss-Prot Project Members, 1991.8.1)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity (Spradling et al., 1999) non-traceable author statement (Swiss-Prot Project Members, 1991.8.1)
Sequence Ontology: Class of Gene
	gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Adf1 - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - CG17118 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - CG32344 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - Gnf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - Gp210 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - Irbp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - Ku80 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - MED4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - RPA2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - RfC4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - TFAM anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - Tom20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - eIF6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Adf1 - spoon anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Adf1 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 70573 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000054 DroID - A comprehensive database of gene and protein interactions. • FBgn0000054 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47082 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P05552
Orthologs
OrthoDB Orthologs (21) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63FHH5 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG61NSGG Arthropod inclusive ortholog search EOG6MCVFR Metazoa inclusive ortholog search EOG67X1JS
Orthologs in Drosophila Species (EOG63FHH5)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Adf1     Drosophila simulans   Dsim\GD10415 Y   Drosophila sechellia   Dsec\GM20885 Y   Drosophila erecta   Dere\GG23211 Y   Drosophila yakuba   Dyak\GE19065 Y   Drosophila ananassae   Dana\GF11060 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA13983 Y   Drosophila persimilis   Dper\GL11108 Y   Drosophila willistoni   Dwil\GK23084 Y   Drosophila virilis   Dvir\GJ22167 Y   Drosophila mojavensis   Dmoj\GI19291 Y   Drosophila grimshawi   Dgri\GH21743 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG61NSGG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Acyrthosiphon pisum Pea aphid  Apim\ACYPI23773 Y Tribolium castaneum Red flour beetle  Tcas\TC009795 Y
Orthologs in non-Insect Arthropods (EOG6MCVFR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_228142 Y Ixodes scapularis Deer tick  Isca\ISCW016166 Y Ixodes scapularis Deer tick  Isca\ISCW017946 Y
Orthologs in non-Arthropod Metazoa (EOG67X1JS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\madf-4 Y Caenorhabditis elegans Nematode  Cele\madf-3 Y Danio rerio Zebrafish  Drer\ENSDARG00000091595 Y Danio rerio Zebrafish  Drer\ENSDARG00000071733 Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10415 Drosophila sechellia Dsec\GM20885 Drosophila erecta Dere\GG23211 Drosophila yakuba Dyak\GE19065 Drosophila ananassae Dana\GF11060 Drosophila pseudoobscura pseudoobscura Dpse\GA13983 Drosophila persimilis Dper\GL11108 Drosophila willistoni Dwil\GK23084 Drosophila virilis Dvir\GJ22167 Drosophila mojavensis Dmoj\GI19291 Drosophila grimshawi Dgri\GH21743
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	28680 y1 v1; P{TRiP.JF03095}attP2 31500 y1 v1; P{TRiP.HM04004}attP2 11040 P{PZ}Adf101349 cn1/CyO, P{sevRas1.V12}FK1
Harvard	- P{XP}Adf1d08233 - PBac{PB}Adf1c05618
Kyoto	123657 w1118; P{RS3}Adf1CB-5490-3 125553 w1118; P{RS5}Adf15-HA-2159 125627 w1118; P{RS5}Adf15-HA-2715 104084 w*; P{GawB}Adf1NP2090 / CyO 102168 y1 w67c23 ; P{lacW}Adf1k00620 / CyO
VDRC	v102176 P{KK111012}VIE-260B v4278 w1118; P{GD1358}v4278
	More stocks available...
Genomic Clones ( 1 )
	BACR01C10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 39 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD09689 RH44336
Other clones	DPiM_LD09689
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH07869 GH11326 GH27983 LD15992 LP20873 RE18869 RE25206 RE25217 RE44087 RE50252 SD07752
Other clones	63601 71717 109364 164186 164201 172608 214577.34 CK01.35C.E6 CK01.109D.G11 CK01.130C.A2 CK01.145C.B4 Dmel_dig_014238_0581_2072 EK043338 EK074747 EK100125 EK119638 EK163620 EK186508 EK190338 EK199751 EK263857 EK268751 EK272544 EK298442 FGM001E12
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000054 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47082
Antibody Information
	polyclonal (England et al., 1992)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Adf1 CG15845 (Bayraktaroglu, 2000.4.12) Source for merge of: Adf-1 l(2)01349 (Spradling et al., 1999)
Additional comments
Other Comments
	Adf1 mutants have defects in larval locomotion. (Parrish et al., 2006) dsRNA has been made from templates generated with primers directed against this gene. RNAi of Adf1 results in increased arborization of ddaD and ddaE neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development. (Parrish et al., 2006) Adf1 may be a critical regulatory switch involved in terminal stages of synapse maturation. (DeZazzo et al., 2000) Comprehensive mapping of the functional domains of Adf1 to study the role of transactivators in the process of gene activation. (Cutler et al., 1998) The specific setting of a nucleosome and the particular histone-DNA contacts over the factor binding sites may play important roles in modulating Adf1 and Trl protein binding to the Adh distal promoter in chromatin to regulate transcription. (Gao and Benyajati, 1998) Shown, by partial sequencing, to be an allele of Adf1. (Tully, 1995) Encodes a transcription factor first identified by its binding to Adh enhancer sequences, but probably quite ubiquitous. The gene was also identified in a P-element screen for learning and memory defective mutations by Tully (FBrf0075143).
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_165479 NM_165480 NM_206028 NM_001273801 RefSeq (Proteins) • NP_724481 NP_724482 NP_995750 NP_001260730 DDBJ / EMBL / GenBank • AA390609 AAA28325 AAF57369 AAF57370 AAL39532 AAS64778 AGB93263 AQ024962 AQ025586 AQ025622 AQ072936 AW942083 AY069387 CZ470134 CZ471168 CZ476144 M37787 Entrez Gene - A searchable database of RefSeq genes. • 47082 UniProtKB/Swiss-Prot • P05552
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • BESS motif (IPR004210) MADF domain (IPR006578) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD09689
Linkouts
	BioGRID - A database of protein and genetic interactions • 70573 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000054 DroID - A comprehensive database of gene and protein interactions. • FBgn0000054 DRSC - Results from RNAi screens. • FBgn0000054 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000054 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG15845-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000054 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 47082 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /gene/adf1-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 47082 modMine - Data generated by the modENCODE project. • FBgn0000054 REDfly - A database of transcriptional regulatory elements. • FBgn0000054 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P05552
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	Adf-1 (Lespinet et al., 2002, Spellman et al., 2001, Spradling et al., 1999, Gao and Benyajati, 1998, Han et al., 1998, Cutler et al., 1998, Guptaroy et al., 1997, Sondergaard, 1993, Geer et al., 1993, England et al., 1992, England et al., 1990, Heberlein et al., 1985, Berkaeva et al., 2007, LANG et al., 2007, GuptaRoy et al., 2000, Dubnau et al., 2003, Zimmermann et al., 2006, Ayroles et al., 2009, Lang and Juan, 2010, Berkaeva et al., 2009) Adf 1 (Kraut et al., 2001, Kraut et al., 2001) adf1 (Sandstrom et al., 1998) Adf1 (Parrish et al., 2006, Aerts et al., 2007, Buszczak et al., 2007, Dubnau et al., 2003, Rendina et al., 2010, Guan et al., 2011, Mortimer and Moberg, 2009, Jungreis et al., 2011, Schaaf et al., 2009, Dierick and Greenspan, 2006, Song et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Antao et al., 2012) adf-1 (Dubnau et al., 2003) Adh transcription factor 1 (Edwards et al., 2006) CG15845 (Kraut et al., 2001, Kraut et al., 2001, Jensen et al., 2006, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2008, Casola et al., 2007) l(2)01349 (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, ) l(2)04065 (BDGP Project Members, 1994-1999, ) nal (Waddell and Quinn, 2001, Roman and Davis, 2001, DeZazzo et al., 2000, DeZazzo et al., 1997, Tully, 1996, Tully, 1995, Guan et al., 2011)
Name Synonym	Adh transcription factor 1 (Edwards et al., 2006, Guan et al., 2011, Jensen et al., 2006) nalyot (Burrell and Sahley, 2001, Waddell and Quinn, 2001, Kraut et al., 2001, Kraut et al., 2001, DeZazzo et al., 2000, DeZazzo et al., 1997, ) Nalyot (Dubnau et al., 2003, Guan et al., 2011) naylot (Roman and Davis, 2001)
Secondary FlyBase IDs
	FBgn0010484 FBgn0010565 FBgn0013714 FBgn0033084
References ( 89 )
Generate a list of	All references relating to this gene ( 89 )
List References by type	Research paper  ( 35 ) Supplementary material  ( 8 ) Review  ( 9 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 14 ) FlyBase analysis  ( 3 ) Letter  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Antao et al., 2012, Mol. Cell. Biol. 32(12): 2170--2182 Protein Landscape at Drosophila melanogaster Telomere-Associated Sequence Repeats. [FBrf0218429] Guan et al., 2011, Learn. Mem. 18(4): 191--206 Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. [FBrf0213277] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...