Adh-dup, BG:DS01486.9 , Adhr
gene_with_dicistronic_mRNA ; SO:0000722
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
Dicistronic transcript isoform(s) appear to be relatively rare based on RNA-Seq and/or EST data.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Adhr using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Adhr in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Adhr CG3484
Dicistronic annotation CG32954 split out into separate annotations for each open reading frame, CG3481 and CG3484, in release 4.2 of the genome annotation. CG3481 corresponds to Adh and CG3484 corresponds to Adhr.
5' end RACE of the Adhr transcript from adults indicates that Adhr is transcribed as a dicistronic mRNA from the Adh distal promoter. RACE experiment with total RNA from embryos showed the embryonic Adhr transcript is also dicistronic and is transcribed from the proximal Adh promoter. Mutations that affect Adh transcripts also affect Adhr transcripts. Sedimentation profiles of polysomes and RNA analysis indicates the Adhr open reading frame of the dicistronic transcript is being translated and strongly suggests that Adhr translation is initiated by internal initiation in the intergenic region between Adh and Adhr coding regions. Adhr protein can be detected and is shown to co-localise with Adh. The level of Adh and Adhr transcripts in su(f) mutant and wild type background demonstrates the accumulation of the dicistronic messenger is controlled by a temperature-sensitive post-transcriptional mechanism.
Flies lacking the Adhr region show morphologically abnormal gastric caecae.
The homologous genomic region containing Adh and Adhr is analysed. Ka and Ks values are determined (Ks values for Adh are significantly lower than values for Adhr) and amino acid comparisons reveal conserved regions shared by Adh and Adhr which have been assigned to known functional domains.
Adhr lies downstream of the Adh region and is a functional gene that is distantly related to Adh by tandem duplication. It consists of 3 non-overlapping reading frames. Adhr is less polymorphic than Adh and all its polymorphisms are at a low frequency suggesting a recent selective substitution in the Adhr region. Natural selection has played an important role in governing the overall patterns of nucleotide variation.
An open reading frame of unknown function located about 300bp proximal (downstream) of Adh. The putative translation product is about 30% similar to Adh in amino acid sequence; in addition the two introns are located at the same positions with respect to the amino acid sequence as are the two introns that interrupt the Adh coding region. These facts lead to the inference that Adhr evolved as a duplication of Adh, or vice versa. The gene, and its position with respect to Adh, is conserved in the subgenus Drosophila and the genus Scaptodrosophila, as well as in obscura species group members.