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General Information
Symbol
Dmel\Mps1
Species
D. melanogaster
Name
Monopolar spindle 1
Annotation Symbol
CG7643
Feature Type
FlyBase ID
FBgn0000063
Gene Model Status
Stock Availability
Enzyme Name (EC)
Dual-specificity kinase (2.7.12.1)
Gene Summary
Monopolar spindle 1 (Mps1) encodes a conserved protein kinase that is associated with kinetochores and is required for the mitotic and meiotic spindle assembly checkpoints. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)
Also Known As

ald, dMps1, altered disjunction

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-61
RefSeq locus
NT_033777 REGION:17671271..17673828
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (24 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
inferred from direct assay
(assigned by UniProt )
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(assigned by GO_Central )
Biological Process (16 terms)
Terms Based on Experimental Evidence (13 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Axs; FB:FBgn0000152
inferred from mutant phenotype
involved_in meiotic cell cycle
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
inferred from direct assay
(assigned by UniProt )
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
located_in kinetochore
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in kinetochore
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(assigned by GO_Central )
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000540737
(assigned by GO_Central )
is_active_in spindle
inferred from biological aspect of ancestor with PANTHER:PTN001122082
(assigned by GO_Central )
Protein Family (UniProt)
-
Catalytic Activity (EC)
Experimental Evidence
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L- seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein] (2.7.12.1)
Predictions / Assertions
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L- seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein] (2.7.12.1)
Summaries
Gene Snapshot
Monopolar spindle 1 (Mps1) encodes a conserved protein kinase that is associated with kinetochores and is required for the mitotic and meiotic spindle assembly checkpoints. [Date last reviewed: 2018-09-06]
Gene Group (FlyBase)
OTHER CONVENTIONAL PROTEIN KINASE DOMAIN KINASES -
The group of 'other conventional protein kinase domain kinases' consists of kinases with a conventional eukaryotic protein kinase domain that do not fit into any of the other major groups. This grouping is described in FBrf0201870.
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
ald: altered disjunction (A.T.C. Carpenter)
Homozygous females display elevated levels of non-disjunction of X and fourth chromosomes (9.5 and 6.0% respectively); double exceptions are predominantly XX;0 and 0;44, products expected from nonhomologous disjunction; behavior of large autosomes nearly normal. Exchange frequencies normal, and sex-chromosome exchange tetrads contribute to exceptional products.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Mps1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry A0A0B4KG66)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Multiphase exon postulated: exon reading frame differs in alternative transcripts; overlap >20aa.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0083478
2113
630
FBtr0331348
2141
672
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0082911
69.8
630
8.44
FBpp0303766
74.3
672
7.59
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mps1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

ald protein localizes to the outer kinetochore in meiotic and mitotic nuclei. It is also observed in cytoplasmic filaments in prometaphase oocytes.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in centrosome
inferred from direct assay
located_in kinetochore
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mps1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 28 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mps1
Transgenic constructs containing regulatory region of Mps1
Aberrations (Deficiencies and Duplications) ( 7 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
anaphase & condensed nuclear chromosome | germ-line clone
chromosome & oocyte (with Mps1ald-1)
chromosome & oocyte (with Mps1C3)
meiosis & nuclear chromosome
meiosis & nuclear chromosome (with Df(3R)ED5780)
spindle microtubule & oocyte (with Mps1ald-1)
spindle microtubule & oocyte (with Mps1C3)
spindle pole & oocyte (with Mps1ald-1)
spindle pole & oocyte (with Mps1C3)
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
1  
1 of 15
No
No
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
5 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
5 of 12
Yes
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (2)
8 of 15
Yes
Yes
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (0)
No records found.
Arabidopsis thaliana (thale-cress) (1)
4 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
11 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
8 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-61
    Cytogenetic map
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    90C1-90C1
    Limits computationally determined from genome sequence between P{PZ}l(3)0788207882 and P{EP}cpoEP3679
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Determined by deficiency mapping followed by SNP mapping.
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (12)
    Genomic Clones (30)
    cDNA Clones (18)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: Mps1 CG7643

      Source for identity of: Mps1 ald

      Source for database merge of

      Source for merge of: ald Mps1

      Additional comments

      The gene symbol "ald" has been changed to "Mps1" to reflect published usage and in order to reduce confusion with the "Ald" ("Aldolase") gene symbol, from which it differed only by case.

      Other Comments

      The rate of meiotic errors in ald mutant females is linked to an increased likelihood of premature release of sister chromatid cohesion, rather than just a shortening of prometaphase duration.

      dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of ald on the spindle checkpoint.

      CG31251- CG31360+ CG31249- Overall orientation not stated: CG14322+ CG7523- ald+ CG18212- CG7655+

      Cytological analysis shows that ald plays a role in the maintenance of the chiasmate homolog associations during meiotic metaphase I.

      When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, decrease in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells and spindle abnormalities are seen.

      RNAi experiments in S2 cells causes no discernable phenotype. Over-expression results in cells displaying abnormal anaphases, with chromatin stretched over the central spindle.

      ald is required for the mitotic spindle checkpoint and is required for the metaphase arrest that results in response to hypoxic conditions.

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      ald kinase plays a role in centrosome duplication and the mitotic spindle checkpoint.

      Mutant females show an increased frequency of nondisjunction of the X and 4th chromosomes.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 43 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (12)
      Reported As
      Symbol Synonym
      Name Synonyms
      Monopolar spindle 1
      altered disjunction
      monopolar spindles 1
      Secondary FlyBase IDs
      • FBgn0038534
      Datasets (8)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (8)
      Result
      Result Type
      Title
      Clustering analysis of hemocytes from non-infested third instar larvae
      Clustering analysis of hemocytes from wasp-infested third instar larvae
      Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
      Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
      Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
      Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
      Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
      Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
      References (92)