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Gene Dmel\ald

General Information
Symbol	Dmel\ald	Species	D. melanogaster
Name	altered disjunction	Annotation symbol	CG7643
Feature type	protein_coding_gene	FlyBase ID	FBgn0000063
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	Mps1, dMps1
Genomic Location
Chromosome (arm)	3R	Recombination map	3-61
Cytogenetic map	90C1-90C1	Sequence location	3R:13,496,993..13,499,550 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene altered disjunction is referred to in FlyBase by the symbol Dmel\ald (CG7643, FBgn0000063). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: protein kinase activity. There is experimental evidence for 12 unique biological process terms, many of which group under: cell cycle; cellular process involved in reproduction; M phase of meiotic cell cycle; cell cycle process; biological regulation; regulation of cellular component organization; response to decreased oxygen levels; response to stress; mitotic cell cycle; response to oxygen levels; negative regulation of mitosis. 36 alleles are reported. The phenotypes of these alleles are annotated with: eye; wing; ommatidium; oocyte. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1637781_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ald. Gene sequence location is 3R:13496993..13499550.
User Contributed Data
	Beta version, feedback welcome
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ald: altered disjunction (A.T.C. Carpenter) Homozygous females display elevated levels of non-disjunction of X and fourth chromosomes (9.5 and 6.0% respectively); double exceptions are predominantly XX;0 and 0;44, products expected from nonhomologous disjunction; behavior of large autosomes nearly normal. Exchange frequencies normal, and sex-chromosome exchange tetrads contribute to exceptional products.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms meiotic spindle assembly checkpoint condensed nuclear chromosome outer kinetochore
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 90C1-90C1   Limits computationally determined from genome sequence between P{PZ}l(3)0788207882 and P{EP}cpoEP3679
Experimentally Determined Cytological Location
Cytogenetic map Notes References 90C1-90C1   Determined by deficiency mapping followed by SNP mapping.   (Gilliland et al., 2005)
Experimentally Determined Recombination Data
Location	3-61
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ald for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Multiphase exon postulated: exon reading frame varies in alternative transcripts. Gene model reviewed during 5.44
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ald-RA FBtr0083478  NM_142387   2113   630 ald-RB FBtr0331348  NM_001275748   2141   672
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ald-PA   FBpp0082911   69.8   630   8.44     NP_650644   AAF55450 ald-PB   FBpp0303766   74.3   672   7.59     NP_001262677   AGB96057
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201533   AA264926   AE014297 AAF55450   AE014297 AGB96057   AW941549   BI577794   BI632289   BI640835   BT010086 AAQ22555   CO299509   CO330628   EC051035   EC052024   EC052533   EC054529   EC055350   EC055794   EC056839   EC217185   EC239433   EV582869
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VEH1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References insertion site PBac{GAL4D,EYFP}ald[PL00781] 3R:13,497,168..13,497,170 comment=The orientation of the insertion was not reported. evidence=experimental (Fischer et al., 2004)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference larval stage     • kinetochore (Gilliland et al., 2007) oogenesis   oocyte   • kinetochore (Gilliland et al., 2007)
Additional Descriptive Data
	ald protein localizes to the outer kinetochore in meiotic and mitotic nuclei. It is also observed in cytoplasmic filaments in prometaphase oocytes. (Gilliland et al., 2007)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
centrosome inferred from direct assay (Althoff et al., 2012) condensed nuclear chromosome outer kinetochore inferred from direct assay (Gilliland et al., 2007) kinetochore inferred from direct assay (Althoff et al., 2012) spindle inferred from direct assay (Althoff et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    51.525 Larval Midgut    1.9 Larval Hindgut    3.4 Larval Malpighian Tubules    3.7 Larval Fat Body    10.9 Larval Salivary Gland    3.6 Larval Trachea    12.125 Larval Carcass    6.35 Adult Head    5.2 Adult Eye    4.425 Adult Brain    12.2 Adult Thoracic-Abdominal Ganglion    11.3 Adult Crop    2.7 Adult Midgut    2.4 Adult Hindgut    3.1 Adult Malpighian Tubules    2.7 Adult Fat Body    3.7 Adult Salivary Gland    1.8 Adult Heart    3.3 Adult VirginFemale Spermatheca    4 Adult InseminatedFemale Spermatheca    3.1 Adult Ovary    112.1 Adult Testis    45.1 Adult Male Accessory Gland    0.8 Adult Carcass    6.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    5.2   NA  Eye    4.425   NA  Brain    12.2   51.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    2.7   1.9  Midgut    2.4   3.4  Hindgut    3.1   3.7  Malpighian Tubules    2.7   no informative data  Fat Body    3.7   3.6  Salivary Gland    1.8   NA  Heart    3.3   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    4   NA  InseminatedFemale Spermatheca    3.1   NA  Ovary    112.1   NA  Testis    45.1   NA  Male Accessory Gland    0.8   6.35  Carcass    6.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    60 ovary, mated 4-day female    62 testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female  (60) ovary, mated 4-day female  (62) testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    60 ovary, mated 4-day female    62 testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    60 ovary, mated 4-day female    62 testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    60 ovary, mated 4-day female    62 testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female  (60) ovary, mated 4-day female  (62) testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    60 ovary, mated 4-day female    62 testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    16 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    2 head, virgin 20-day female    1 head, mated 1-day female    2 head, mated 4-day female    1 head, mated 20-day female    2 head, mated 1-day male    1 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    2 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    3 carcass, 20-day adult    2 ovary, virgin 4-day female    60 ovary, mated 4-day female    62 testis, mated 4-day male    12 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (35) embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (35) embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    35 embryo 02-04hr    9 embryo 04-06hr    15 embryo 06-08hr    15 embryo 08-10hr    8 embryo 10-12hr    3 embryo 12-14hr    3 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    0 embryo 22-24hr    1 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    4 larva L3 puffstage 7-9    5 white prepupae new    5 white prepupae 12hr    7 white prepupae 24hr    7 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    2 adult male 01day    3 adult male 05day    4 adult male 30day    4 adult female 01day    7 adult female 05day    19 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1    30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS    35 ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1  (30) embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9  26 wing disc ML-DmD16-c3  26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1  (32) CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS  (35) ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1    30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS    35 ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1    30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS    35 ovary OSC    22 ovary OSS    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1    30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS    35 ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1  30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9  26 wing disc ML-DmD16-c3  26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1  32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS  (35) ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1    30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS    35 ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    21 Schneider line 2 Sg4    15 embryonic 1182-4H    15 embryonic GM2    17 embryonic Kc167    18 embryonic S1    30 embryonic S3    14 leg disc CME L1    19 wing disc CME-W2    13 wing disc ML-DmD8    18 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    26 wing disc ML-DmD21    11 wing disc ML-DmD32    19 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    9 ovary fGS/OSS    35 ovary OSC    22 ovary OSS    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    37 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult  (42) Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3  (33) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (37) Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult  (32) Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    37 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    37 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    37 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult  (42) Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3  (33) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (37) Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult  32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    37 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    12 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    15 Cadmium 100 mM 48 hrs, 4-day adult    42 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    22 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    33 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    3 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    37 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    32 Paraquat 10 mM 48 hrs, 4-day adult    20 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	BDGP expression data • LD04521 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000063 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7643-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage aldB4 aldC3 lethal | pharate adult stage | recessive aldPL00781 partially lethal - majority die aldC3 viable aldExc23 viable (with Df(3R)ED5780) aldexcision33 aldGS13084 Sterility Allele fertile aldExc23 fertile (with Df(3R)ED5780) aldGS13084 Other Phenotypes Allele meiotic cell cycle defective ald1 aldC3 meiotic cell cycle defective (with aldC3) aldExc23 meiotic cell cycle defective (with aldExc23) aldC3 meiotic cell cycle defective (with Df(3R)AN6) ald1 aldExc23 meiotic cell cycle defective | female ald1 aldGS13084 meiotic cell cycle defective | female (with Df(3R)ED5780) ald1 aldGS13084 meiotic cell cycle defective | female | germline clone aldA15 mitotic cell cycle defective | embryonic stage 5, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 aldCenp-C.Scer\UAS.T:Avic\GFP-EGFP aldNuf2.Scer\UAS.T:Avic\GFP-EGFP aldScer\UAS.cAa mitotic cell cycle defective | embryonic stage 5 | conditional, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 aldScer\UAS.T:Avic\GFP-EGFP Phenotype manifest in Allele anaphase & condensed nuclear chromosome | germ-line clone aldPL00781 chromosome & oocyte (with ald1) aldC3 chromosome & oocyte (with aldC3) ald1 eye aldPL00781 kinetochore | embryonic stage 5, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 aldCenp-C.Scer\UAS.T:Avic\GFP-EGFP meiosis & nuclear chromosome ald1 aldGS13084 meiosis & nuclear chromosome (with Df(3R)ED5780) ald1 aldGS13084 mitotic cell cycle | germline clone aldPL00781 nucleus | germline clone aldPL00781 ommatidium aldPL00781 oocyte ald1 aldC3 oocyte (with aldC3) aldExc23 oocyte (with aldExc23) aldC3 oocyte (with Df(3R)AN6) ald1 aldExc23 polar body nucleus | germline clone aldPL00781 spindle microtubule & oocyte (with ald1) aldC3 spindle microtubule & oocyte (with aldC3) ald1 spindle pole & oocyte (with ald1) aldC3 spindle pole & oocyte (with aldC3) ald1 wing aldPL00781
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of ald Class Mutagen Stocks Known lesion aldG4422 P-element activity 1 -- ald1 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes ald3-13 0 -- ald6-11 0 -- aldA15 ethyl methanesulfonate 0 -- aldB4 0 Yes aldB8 0 -- aldC3 loss of function allele 0 Yes aldExc23 P-element activity 0 Yes aldexcision33 P-element activity 0 Yes aldGS13084 hypomorphic allele - genetic evidence P-element activity 0 Yes aldPL00781 0 Yes
Alleles Carried on Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ald Class Mutagen Stocks Known lesion aldGL00184 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes aldHMS01546 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ald+t4.2 in vitro construct - genomic fragment 0 Yes aldC.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes aldC.T:Avic\GFP-EGFP in vitro construct - coding region fusion in vitro construct - deletion 0 Yes aldCenp-C.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes aldCkd.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes aldGD8895 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes aldkd.Cenp-C.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes aldkd.Nuf2.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes aldkd.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes aldkd.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes aldkd.T:Avic\GFP-EGFP in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes aldKK102940 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes aldN.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes aldN.T:Avic\GFP-EGFP in vitro construct - coding region fusion in vitro construct - deletion 0 Yes aldNIG.7643R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes aldNuf2.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes aldScer\UAS.cAa in vitro construct - regulatory fusion 0 Yes aldScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes aldScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes aldT:Avic\GFP-EGFP in vitro construct - coding region fusion 0 Yes aldTor4021.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes aldTorEC-TM.Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Duplicated in	Dp(3;3)cpo1.36 (Schmidt et al., 2008)
Disrupted in	Df(3R)AN6 (Page et al., 2007) Df(3R)cpo1.45 (Schmidt et al., 2008) Df(3R)ED5780 (Gilliland et al., 2005) Df(3R)Exel6177 (Ryder, 2004.4.26) Df(3R)RSH1 (Gilliland et al., 2005)
Not disrupted in	Df(3R)AN6 (Gilliland et al., 2007) Df(3R)RS2-53 (Gilliland et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8895} NA P{KK102940} NA P{NIG.7643R} NA P{TRiP.GL00184} NA P{TRiP.HMS01546} NA P{UAS-ald.A} NA P{UAS-ald.C.EGFP} NA P{UAS-ald.Cenp-C.EGFP} NA P{UAS-ald.Cenp-C.kd.EGFP} NA P{UAS-ald.Ckd.EGFP} NA P{UAS-ald.EGFP} NA P{UAS-ald.kd.EGFP} NA P{UAS-ald.kd.Nuf2.EGFP} NA P{UAS-ald.MYC} NA P{UAS-ald.N.EGFP} NA P{UAS-ald.Nuf2.EGFP} NA P{UAS-ald.Tor4021.EGFP} NA P{UAS-ald.TorEC-TM.EGFP} NA characterization construct P{ald-EGFP.C} NA P{ald-EGFP.kd} NA P{ald-EGFP.N} NA P{EGFP-Mps1.F} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system PBac{GAL4D,EYFP}aldPL00781 No insertion of mobile activating element P{EP}aldG4422 NA P{GSV6}aldGS13084 NA miscellaneous insertions Doc{}altA15 NA Doc{}altB8 NA
Gene Ontology: Function, Process & Cellular Component ( 21 unique terms )
Terms Based on Experimental Evidence ( 17 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
achiasmate meiosis I inferred from mutant phenotype (Gilliland et al., 2005) distributive segregation inferred from genetic interaction with Axs (Whyte et al., 1993) inferred from mutant phenotype (Whyte et al., 1993) female meiosis inferred from mutant phenotype (Gilliland et al., 2007) female meiosis chromosome segregation inferred from mutant phenotype (Gilliland et al., 2005, O'Tousa, 1982) meiosis inferred from mutant phenotype (Page et al., 2007) meiotic metaphase I plate congression inferred from mutant phenotype (Gilliland et al., 2005) meiotic spindle assembly checkpoint inferred from mutant phenotype (Gilliland et al., 2007) mitotic cell cycle inferred from mutant phenotype (Faria et al., 2004) NOT mitotic centrosome separation inferred from mutant phenotype (Faria et al., 2004) mitotic spindle assembly checkpoint inferred from mutant phenotype (Fischer et al., 2004) negative regulation of sister chromatid segregation inferred from mutant phenotype (Althoff et al., 2012) response to hypoxia inferred from mutant phenotype (Fischer et al., 2004) spindle checkpoint inferred from mutant phenotype (Gilliland et al., 2005)
Cellular Component
CV term Evidence References
centrosome inferred from direct assay (Althoff et al., 2012) condensed nuclear chromosome outer kinetochore inferred from direct assay (Gilliland et al., 2007) kinetochore inferred from direct assay (Althoff et al., 2012) spindle inferred from direct assay (Althoff et al., 2012)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from electronic annotation with InterPro:IPR008271 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
female meiosis chromosome segregation traceable author statement (Wayson et al., 2000) protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR008271 (FlyBase Curators et al., 2004-) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
ald - Mad1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) ald - mad2 experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ald allele Gene References unspecified Axs
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67158 DroID - A comprehensive database of gene and protein interactions. • FBgn0000063 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42126 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VEH1
Orthologs
OrthoDB Orthologs (46) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VQB81 Dipteran inclusive ortholog search EOG60GD08 Insect inclusive ortholog search EOG6HDRPC Arthropod inclusive ortholog search EOG6K98T3 Metazoa inclusive ortholog search EOG6VQPG4
Orthologs in Drosophila Species (EOG6VQB81)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ald     Drosophila simulans   Dsim\GD19180 Y   Drosophila sechellia   Dsec\GM15256 Y   Drosophila erecta   Dere\GG22427 Y   Drosophila yakuba   Dyak\GE25491 Y   Drosophila ananassae   Dana\GF17824 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20501 Y   Drosophila persimilis   Dper\GL21521 Y   Drosophila willistoni   Dwil\GK13492 Y   Drosophila virilis   Dvir\GJ22665 Y   Drosophila mojavensis   Dmoj\GI24602 Y   Drosophila grimshawi   Dgri\GH18102 Y
Orthologs in non-Drosophila Dipterans (EOG60GD08)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011118   Anopheles gambiae Malaria mosquito  Agam\AGAP002075   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013391
Orthologs in non-Dipteran Insects (EOG6HDRPC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49084   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005277   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10170   Atta cephalotes Leafcutter ant  Acep\ACEP20725   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15161   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL12502   Linepithema humile Argentine ant  Lhum\LH23405   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15580   Solenopsis invicta Red fire ant  Sinv\SINV18123   Acyrthosiphon pisum Pea aphid  Apim\ACYPI069389   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005149   Bombyx mori Silkmoth  Bmor\BGIBMGA007634   Pediculus humanus Human body louse  Phum\PHUM357590   Tribolium castaneum Red flour beetle  Tcas\ald
Orthologs in non-Insect Arthropods (EOG6K98T3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_321091   Daphnia pulex Water flea  Dpux\flaB3   Daphnia pulex Water flea  Dpux\JGI_V11_332607   Daphnia pulex Water flea  Dpux\JGI_V11_327913   Daphnia pulex Water flea  Dpux\JGI_V11_14361   Daphnia pulex Water flea  Dpux\JGI_V11_119424   Daphnia pulex Water flea  Dpux\JGI_V11_325381   Daphnia pulex Water flea  Dpux\matK   Ixodes scapularis Deer tick  Isca\ISCW019686   Ixodes scapularis Deer tick  Isca\ISCW007211
Orthologs in non-Arthropod Metazoa (EOG6VQPG4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010530gn   Danio rerio Zebrafish  Drer\ttk   Xenopus tropicalis Western clawed frog  Xtro\ttk   Gallus gallus Domestic chicken  Ggal\TTK   Mus musculus House mouse  Mmus\ENSMUSG00000038379   Rattus norvegicus Norway rat  Rnor\Ttk   Homo sapiens Human  Hsap\TTK
Human Orthologs (1)
Gene OMIM HGNC Hsap\TTK
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19180 Drosophila sechellia Dsec\GM15256 Drosophila erecta Dere\GG22427 Drosophila yakuba Dyak\GE25491 Drosophila ananassae Dana\GF17824 Drosophila pseudoobscura pseudoobscura Dpse\GA20501 Drosophila persimilis Dper\GL21521 Drosophila willistoni Dwil\GK13492 Drosophila virilis Dvir\GJ22665 Drosophila mojavensis Dmoj\GI24602 Drosophila grimshawi Dgri\GH18102
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	30079 w1118; P{EP}aldG4422 35283 y1 sc* v1; P{TRiP.GL00184}attP2 36658 y1 sc* v1; P{TRiP.HMS01546}attP2/TM3, Sb1
Genomic Clones ( 3 )
	BACR11L13 BACR14A01 BACR21K03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD04521
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD08595 RE71539 SD23843 SD26030
Other clones	192055 CK01.1D.G10 CK01.117C.C6 Dmel_dig_005961_0619_2543 EC051035 EC052024 EC052533 EC054529 EC055350 EC055794 EK175705 EK295757
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000063 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42126
Antibody Information
	polyclonal (Gilliland et al., 2007)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Mps1 CG7643 (Faria, 2004.2.23)
Source for database merge of	Source for merge of: ald Mps1 (Gilliland et al., 2005)
Additional comments
Other Comments
	The rate of meiotic errors in ald mutant females is linked to an increased likelihood of premature release of sister chromatid cohesion, rather than just a shortening of prometaphase duration. (Gilliland et al., 2007) dsRNA against this gene has been used to treat SL2 cells to study the effect of depletion of ald on the spindle checkpoint. (Muller et al., 2006) CG31251- CG31360+ CG31249- Overall orientation not stated: CG14322+ CG7523- ald+ CG18212- CG7655+ (Gilliland et al., 2005) Cytological analysis shows that ald plays a role in the maintenance of the chiasmate homolog associations during meiotic metaphase I. (Gilliland et al., 2005) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, decrease in mitotic index, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells and spindle abnormalities are seen. (Bettencourt-Dias et al., 2004) RNAi experiments in S2 cells causes no discernable phenotype. Over-expression results in cells displaying abnormal anaphases, with chromatin stretched over the central spindle. (Faria et al., 2004) ald is required for the mitotic spindle checkpoint and is required for the metaphase arrest that results in response to hypoxic conditions. (Fischer et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) ald kinase plays a role in centrosome duplication and the mitotic spindle checkpoint. (Lehner, 2003) Mutant females show an increased frequency of nondisjunction of the X and 4th chromosomes.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142387 NM_001275748 RefSeq (Proteins) • NP_650644 NP_001262677 DDBJ / EMBL / GenBank • AA201533 AAF55450 AAQ22555 AGB96057 AW941549 BT010086 Entrez Gene - A searchable database of RefSeq genes. • 42126 UniProtKB/TrEMBL • Q9VEH1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD04521
Linkouts
	BioGRID - A database of protein and genetic interactions • 67158 DroID - A comprehensive database of gene and protein interactions. • FBgn0000063 DRSC - Results from RNAi screens. • FBgn0000063 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000063 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7643-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000063 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42126 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42126 modMine - Data generated by the modENCODE project. • FBgn0000063 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VEH1
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	ald (Gilliland et al., 2007, Gilliland et al., 2007, Whyte et al., 1993, Hawley and Gilliland, 2006, Georlette et al., 2007, Wu et al., 2008, Anderson et al., 2009, Hughes et al., 2009, Page et al., 2007, Reis et al., 2011, Japanese National Institute of Genetics, 2012.5.21) Ald/Mps1 (Gilliland et al., 2009) CG7643 (Gilliland et al., 2005, Bettencourt-Dias et al., 2004, Fischer et al., 2004, Manning et al., 2002, Asha et al., 2003, Gilliland et al., 2005, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) dMps1 (Faria et al., 2004, Faria et al., 2005) Mps1 (Muller et al., 2006, Gilliland et al., 2005, Buffin et al., 2005, Hacker, 2005.4.28, Fisk and Winey, 2004, Fischer et al., 2004, Manning et al., 2002, Faria, 2004.2.23, Lehner et al., 2007, Schuh et al., 2007, Schuh et al., 2007, Wen et al., 2008, Pandey et al., 2007, Lince-Faria et al., 2009, Althoff et al., 2009, Gilliland et al., 2009, Emre et al., 2011) mps1 (Gilliland et al., 2007, Gilliland et al., 2007, Hawley and Gilliland, 2006, Page et al., 2007, Batiha and Swan, 2012) Msp1 (Althoff et al., 2012) TKK (Morrison et al., 2000)
Name Synonym	altered disjunction (Page et al., 2007) monopolar spindles 1 (Gilliland et al., 2007)
Secondary FlyBase IDs
	FBgn0038534
References ( 69 )
Generate a list of	All references relating to this gene ( 69 )
List References by type	Research paper  ( 28 ) Supplementary material  ( 7 ) Review  ( 8 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 10 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 6 )
	Gillies et al., 2013, Genetics 193(2): 443--451 Nondisjunctional segregations in Drosophila female meiosis I are preceded by homolog malorientation at metaphase arrest. [FBrf0220742] Althoff et al., 2012, Mol. Biol. Cell 23(12): 2275--2291 Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1. [FBrf0218626] Batiha and Swan, 2012, Genome 55(1): 63--67 Evidence that the spindle assembly checkpoint does not regulate APC(Fzy) activity in Drosophila female meiosis. [FBrf0217126] Herriott et al., 2012, Cell Cycle 11(24): 4650--4660 Kinetochore localized Mad2 and Cdc20 is itself insufficient for triggering the mitotic checkpoint when Mps1 is low in Drosophila melanogaster neuroblasts. [FBrf0220451] Emre et al., 2011, J. Cell Sci. 124(10): 1664--1671 A mitotic role for Mad1 beyond the spindle checkpoint. [FBrf0213557] Reis et al., 2011, PLoS ONE 6(3): e17512 Drosophila Genes That Affect Meiosis Duration Are among the Meiosis Related Genes That Are More Often Found Duplicated. [FBrf0213266] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...