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General Information
Symbol
Dmel\Ald1
Species
D. melanogaster
Name
Aldolase 1
Annotation Symbol
CG6058
Feature Type
FlyBase ID
FBgn0000064
Gene Model Status
Stock Availability
Enzyme Name (EC)
Fructose-bisphosphate aldolase (4.1.2.13)
Gene Summary
May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. Protein acts on two substrates fructose 1,6-bisphosphate and fructose 1-phosphate (like other class I aldolases). (UniProt, P07764)
Contribute a Gene Snapshot for this gene.
Also Known As

Ald, Aldolase

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
3-90
RefSeq locus
NT_033777 REGION:26254069..26261591
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000179343
(assigned by GO_Central )
Biological Process (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from mutant phenotype
involved_in glycolytic process
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000179343
(assigned by GO_Central )
involved_in glycolytic process
inferred from biological aspect of ancestor with PANTHER:PTN000179343
(assigned by GO_Central )
non-traceable author statement
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
located_in M band
inferred from direct assay
located_in Z disc
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cytosol
inferred from biological aspect of ancestor with PANTHER:PTN000179343
(assigned by GO_Central )
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the class I fructose-bisphosphate aldolase family. (P07764)
Catalytic Activity (EC)
Experimental Evidence
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (4.1.2.13)
Predictions / Assertions
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (4.1.2.13)
Summaries
Gene Group (FlyBase)
ALDEHYDE-LYASES -
Aldehyde-lyases catalyze the cleavage of carbon-carbon bond in alcohols into carbanions and aldehydes. The vast majority are aldolases, involved in carbohydrate metabolism. (Adapted from FBrf0238856).
Protein Function (UniProtKB)
May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. Protein acts on two substrates fructose 1,6-bisphosphate and fructose 1-phosphate (like other class I aldolases).
(UniProt, P07764)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
Ald: Aldolase
Structural gene for fructose-biphosphate aldolase (EC 4.1.2.13). Enzyme multimeric based on the formation of heteromultimeric bands on gels from heterozygotes for electrophoretic variants. Amino-acid sequence determined; protein is a 158-kd multimer comprising four 360-amino-acid polypeptides. Monomers show 71% identity with rabbit muscle aldolase (Malek, Suter, Frank, and Brenner-Holzach, 1985, Biochem. Biophys. Res. Comm. 126: 199-205). Mixed multimers of rabbit and Drosophila subunits able to function (Brenner-Holzach and Leuthard, 1972, Eur. J. Biochem. 31: 423-26). Sequence analysis suggests alternating domains of alpha helix and beta sheet; domain boundaries correspond to boundaries between exons as seen in rat-liver aldolase (Sawyer, Fothergill-Gilmore, and Freemont, 1988, Biochem. J. 249: 789-93).
Gene Model and Products
Number of Transcripts
10
Number of Unique Polypeptides
3

Please see the JBrowse view of Dmel\Ald1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry P07764)

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Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.43

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.49

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084994
2636
361
FBtr0084995
1279
361
FBtr0084999
1483
361
FBtr0085000
1747
363
FBtr0085001
1618
363
FBtr0114544
1736
363
FBtr0306657
1614
363
FBtr0310660
1472
361
FBtr0310661
1615
363
FBtr0339614
2102
361
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0084367
39.0
361
7.49
FBpp0084368
39.0
361
7.49
FBpp0084372
39.0
361
7.49
FBpp0084373
39.6
363
7.10
FBpp0089214
39.6
363
7.87
FBpp0113036
39.6
363
7.10
FBpp0297612
39.6
363
7.87
FBpp0302780
39.0
361
7.49
FBpp0302781
39.6
363
7.87
FBpp0308680
39.0
361
7.49
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

361 aa isoforms: Ald1-PB, Ald1-PC, Ald1-PD, Ald1-PK, Ald1-PM
363 aa isoforms: Ald1-PE, Ald1-PI
363 aa isoforms: Ald1-PH, Ald1-PJ, Ald1-PL
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Homotetramer.

(UniProt, P07764)
Crossreferences
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ald1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Additional Descriptive Data

Ald transcript levels are unaltered by dietary variations in the level of glucose, fructose, or starch. A small increase in transcript level is seen on 5% sucrose vs. 0.3% sucrose. Ald transcript levels increase slightly in response to starch.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Glycolytic enzymes, including Gpdh protein are localized to M bands and Z discs, as visualized in the isolated myofibrils of flight muscles, where immunoreactivity appears as alternating bright and less-intense bands.

Ald enzyme levelsare not significantly influenced by dietary sucrose, glucose, or fructose.Activity was slightly elevated in response to starch. Ald levelsdecrease slightly on 9% ethanol compared to 1% ethanol.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
located_in M band
inferred from direct assay
located_in Z disc
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ald1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Ald1
Transgenic constructs containing regulatory region of Ald1
Aberrations (Deficiencies and Duplications) ( 4 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
13 of 15
Yes
Yes
1  
13 of 15
Yes
Yes
11 of 15
No
Yes
Hsap\LOC112694756
2 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
14 of 15
Yes
Yes
13 of 15
No
Yes
11 of 15
No
Yes
10 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
13 of 13
Yes
Yes
12 of 13
No
Yes
11 of 13
No
Yes
9 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
12 of 12
Yes
Yes
8 of 12
No
Yes
7 of 12
No
Yes
Danio rerio (Zebrafish) (5)
13 of 15
Yes
Yes
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
10 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
11 of 15
Yes
Yes
10 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (8)
9 of 9
Yes
Yes
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
7 of 9
No
Yes
6 of 9
No
Yes
5 of 9
No
Yes
5 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
7 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Homotetramer.
(UniProt, P07764 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-90
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
97A6-97A6
Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
97B-97B
(determined by in situ hybridisation)
97A-97B
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (15)
Genomic Clones (25)
cDNA Clones (391)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Ald1 Ald

Source for database merge of

Source for merge of: Ald BcDNA:LP07735

Additional comments

Source for merge of Ald BcDNA:LP07735 was a shared cDNA ( date:030728 ).

Other Comments

Area matching Drosophila Fructose 1,6 bisphosphare aldolase gene, Acc. No. M98351.

At least a substantial fraction of three glycolytic enzymes of flight muscle cells are found colocalised at the Z-discs and M-lines, glycolytic enzyme colocalisation is interdependent. Localisation of Gapdh1, Gapdh2 and Ald at the Z-discs and M-lines requires the presence of at least Gpdh at the Z-discs and M-lines.

The structural gene for aldolase (fructose-biphosphate aldolase).

Origin and Etymology
Discoverer
Etymology

Renamed from 'Ald' to 'Ald1' in recognition that there's a second Aldolase gene (Ald2/CG5432).

Identification
External Crossreferences and Linkouts ( 116 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
modMine - A data warehouse for the modENCODE project
PDB - An information portal to biological macromolecular structures
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (17)
Reported As
Symbol Synonym
BcDNA:LP07735
Secondary FlyBase IDs
  • FBgn0064914
Datasets (8)
Study focus (0)
Experimental Role
Project
Project Type
Title
Study result (8)
Result
Result Type
Title
Clustering analysis of hemocytes from non-infested third instar larvae
Clustering analysis of hemocytes from wasp-infested third instar larvae
Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
References (166)