General Information
Symbol
Dmel\Ald1
Species
D. melanogaster
Name
Aldolase 1
Annotation Symbol
CG6058
Feature Type
FlyBase ID
FBgn0000064
Gene Model Status
Stock Availability
Enzyme Name (EC)
Fructose-bisphosphate aldolase (4.1.2.13)
Gene Snapshot
In progress.Contributions welcome.
Also Known As
Ald
Genomic Location
Cytogenetic map
Sequence location
3R:26,254,069..26,261,591 [-]
Recombination map
3-90
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the class I fructose-bisphosphate aldolase family. (P07764)
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
-
Catalytic Activity (EC)
Experimental Evidence
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (4.1.2.13)
Predictions / Assertions
-
Summaries
Gene Group Membership
ALDEHYDE-LYASES -
Aldehyde-lyases catalyze the cleavage of carbon-carbon bond in alcohols into carbanions and aldehydes. The vast majority are aldolases, involved in carbohydrate metabolism. (Adapted from FBrf0238856).
UniProt Contributed Function Data
May take part in developmental stage-specific or tissue -specific sugar-phosphate metabolisms. Protein acts on two substrates fructose 1,6-bisphosphate and fructose 1-phosphate (like other class I aldolases).
(UniProt, P07764)
Phenotypic Description from the Red Book (Lindsley and Zimm 1992)
Ald: Aldolase
Structural gene for fructose-biphosphate aldolase (EC 4.1.2.13). Enzyme multimeric based on the formation of heteromultimeric bands on gels from heterozygotes for electrophoretic variants. Amino-acid sequence determined; protein is a 158-kd multimer comprising four 360-amino-acid polypeptides. Monomers show 71% identity with rabbit muscle aldolase (Malek, Suter, Frank, and Brenner-Holzach, 1985, Biochem. Biophys. Res. Comm. 126: 199-205). Mixed multimers of rabbit and Drosophila subunits able to function (Brenner-Holzach and Leuthard, 1972, Eur. J. Biochem. 31: 423-26). Sequence analysis suggests alternating domains of alpha helix and beta sheet; domain boundaries correspond to boundaries between exons as seen in rat-liver aldolase (Sawyer, Fothergill-Gilmore, and Freemont, 1988, Biochem. J. 249: 789-93).
Gene Model and Products
Number of Transcripts
10
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\Ald1 or the JBrowse view of Dmel\Ald1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.43
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.49
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0084994
2636
361
FBtr0084995
1279
361
FBtr0084999
1483
361
FBtr0085000
1747
363
FBtr0085001
1618
363
FBtr0114544
1736
363
FBtr0306657
1614
363
FBtr0310660
1472
361
FBtr0310661
1615
363
FBtr0339614
2102
361
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0084367
39.0
361
7.49
FBpp0084368
39.0
361
7.49
FBpp0084372
39.0
361
7.49
FBpp0084373
39.6
363
7.10
FBpp0089214
39.6
363
7.87
FBpp0113036
39.6
363
7.10
FBpp0297612
39.6
363
7.87
FBpp0302780
39.0
361
7.49
FBpp0302781
39.6
363
7.87
FBpp0308680
39.0
361
7.49
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

361 aa isoforms: Ald1-PB, Ald1-PC, Ald1-PD, Ald1-PK, Ald1-PM
363 aa isoforms: Ald1-PE, Ald1-PI
363 aa isoforms: Ald1-PH, Ald1-PJ, Ald1-PL
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Homotetramer.
(UniProt, P07764)
Crossreferences
PDB - An information portal to biological macromolecular structures
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ald1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (7 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
non-traceable author statement
Cellular Component (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Transcript Expression
Additional Descriptive Data
Ald transcript levels are unaltered by dietary variations in the level of glucose, fructose, or starch. A small increase in transcript level is seen on 5% sucrose vs. 0.3% sucrose. Ald transcript levels increase slightly in response to starch.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Glycolytic enzymes, including Gpdh protein are localized to M bands and Z discs, as visualized in the isolated myofibrils of flight muscles, where immunoreactivity appears as alternating bright and less-intense bands.
Ald enzyme levelsare not significantly influenced by dietary sucrose, glucose, or fructose.Activity was slightly elevated in response to starch. Ald levelsdecrease slightly on 9% ethanol compared to 1% ethanol.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ald1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 12 )
Transgenic Constructs ( 10 )
Deletions and Duplications ( 4 )
Summary of Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
13 of 15
No
Yes
11 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
14 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
8 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
12 of 13
Yes
Yes
12 of 13
Yes
Yes
11 of 13
No
Yes
9 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (3)
11 of 12
Yes
Yes
7 of 12
No
Yes
7 of 12
No
Yes
Danio rerio (Zebrafish) (5)
13 of 15
Yes
Yes
13 of 15
Yes
Yes
12 of 15
No
Yes
11 of 15
No
Yes
11 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
11 of 15
Yes
Yes
10 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (8)
9 of 9
Yes
Yes
9 of 9
Yes
Yes
8 of 9
No
Yes
8 of 9
No
Yes
6 of 9
No
Yes
5 of 9
No
Yes
4 of 9
No
Yes
4 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190AKG )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505WS )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05VP )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X071J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0A9T )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please look at the Interaction Group reports for full details of the physical interactions
    protein-protein
    Interacting group
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homotetramer.
    (UniProt, P07764 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-90
    Cytogenetic map
    Sequence location
    3R:26,254,069..26,261,591 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    97A6-97A6
    Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    97B-97B
    (determined by in situ hybridisation)
    97A-97B
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (18)
    Genomic Clones (25)
    cDNA Clones (391)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for identity of: Ald1 Ald
    Source for database merge of
    Source for merge of: Ald BcDNA:LP07735
    Additional comments
    Source for merge of Ald BcDNA:LP07735 was a shared cDNA ( date:030728 ).
    Other Comments
    Area matching Drosophila Fructose 1,6 bisphosphare aldolase gene, Acc. No. M98351.
    At least a substantial fraction of three glycolytic enzymes of flight muscle cells are found colocalised at the Z-discs and M-lines, glycolytic enzyme colocalisation is interdependent. Localisation of Gapdh1, Gapdh2 and Ald at the Z-discs and M-lines requires the presence of at least Gpdh at the Z-discs and M-lines.
    The structural gene for aldolase (fructose-biphosphate aldolase).
    Origin and Etymology
    Discoverer
    Etymology
    Renamed from 'Ald' to 'Ald1' in recognition that there's a second Aldolase gene (Ald2/CG5432).
    Identification
    External Crossreferences and Linkouts ( 106 )
    Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    PDB - An information portal to biological macromolecular structures
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc - Genes from a BioCyc PGDB for Dmel
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    FlyMine - An integrated database for Drosophila genomics
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Synonyms and Secondary IDs (16)
    Reported As
    Secondary FlyBase IDs
    • FBgn0064914
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (145)