FlyBase Gene Report: Dmel\Aldox89A

General Information

Symbol

Dmel\Aldox89A

Species

D. melanogaster

Name

Aldehyde oxidase at 89A

Annotation Symbol

Feature Type

gene

FlyBase ID

FBgn0000065

Gene Model Status

Unannotated

Stock Availability

9 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Red Book
Interactive Fly

Also Known As

Aldox, Ao

Function

Gene Ontology (GO) Annotations (0 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Aldox-1: Aldehyde oxidase-1

Structural gene for aldehyde oxidase [AO-1 (EC 1.2.3.1)]. A molybdenum-containing homodimer with subunits of 140,000-dalton molecular weight. As with other molybdenum hydroxylases, activity is inhibited by tungsten and depends on the presence of a low-molecular-weight cofactor (Warner and Finnerty). Enzyme activity increases nonuniformly during development with step increases at pupation and midway through pupal stage. First increase appears to be controlled by a closely linked cis-acting element (Dickinson, 1975, Dev. Biol. 42: 131-40) and the latter by Aldox-2+ (Bentley and Williamson, 1979, Z. Naturforsch. 34: 304-05). Control independent of that of lpo located 0.08 unit to the left (Dickinson and Weisbrod, 1976, Biochem. Genet. 14: 709-21). Enzyme activity absent in cin (Browder and Williamson, 1976, Develop. Biol. 53: 241-49) and mal and reduced in lxd (Courtright, 1967, Genetics 57: 25-39); cross-reacting material observed in all three genotypes (Browder, Wilkes, and Tucker, 1982, Biochem. Genet. 20: 111-24). These mutants presumably affect the availability of molybdenum cofactor (Warner and Finnerty). Autonomous in transplants. Enzyme composition in egg and early larva reflects maternal genotype, giving way to that of zygotic genotype during larval life. Tissue specificity varies with stage and strain (Dickinson, 1972, Genetics 71: s14; Cypher, Tedesco, Courtright and Kumaran, 1982, Biochem. Genet. 20: 315-32). Heptaldehyde serves as specific substrate (Cypher et al.). Differential staining for enzyme noted in different compartments of the wing imaginal disc (Kuhn and Cunningham, 1976, Genetics 83: s42).

Aldox-1Pa1

Exhibits substantial increase in enzyme activity between late larval and early pupal stages followed by a second increase in late pupal stage. Results in relatively high pupal:adult activity ratio.

Aldox-1Pa2

Exhibits little change in enzyme activity between late larval and early pupal stages, but there is a substantial increase in late pupal and adult stages. Results in relatively low pupal:adult activity ratio. Aldox-1Pa1/Aldox-1Pa2 has intermediate phenotype.

Aldox-1Pb1

Cytochemically, enzyme activity in paragonia uniformly distributed and high at eclosion. By end of first week of adult life enzyme accumulated into intracellular bodies giving accessory gland a spotted appearance.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Transcript Data

Annotated Transcripts

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Polypeptides with Identical Sequences

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Aldox89A using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Aldox89A in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Aldox89A

View exonic expression by tissue for Dmel\Aldox89A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 37 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 0 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Aldox89A

Transgenic constructs containing regulatory region of Aldox89A

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene duplicated in

Dp(3;1)ry³⁵

Gene disrupted in

Df(3R)Aldoxⁿ¹

(van Zijll Langhout and van Breugel, 1985)

Df(3R)Po2

(Nelson and Szauter, 1992)

Df(3R)Po3

(Nelson and Szauter, 1992)

Df(3R)Po4

(Nelson and Szauter, 1992)

Df(3R)sbd105

(Nelson and Szauter, 1992)

Gene not disrupted in

Df(3R)c(3)G-2

(Nelson and Szauter, 1992)

Df(3R)sbd26

(Nelson and Szauter, 1992)

Df(3R)sbd45

(Nelson and Szauter, 1992)

Inferred from location ( 0 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Other Phenotypes

Allele

electrophoretic variant | fast

Aldox89A³

electrophoretic variant | slow

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (0)

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (0)

Mus musculus (laboratory mouse) (0)

Xenopus tropicalis (Western clawed frog) (0)

Danio rerio (Zebrafish) (0)

Caenorhabditis elegans (Nematode, roundworm) (0)

Anopheles gambiae (African malaria mosquito) (0)

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Aldox89A. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (0)

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-57.2

Cytogenetic map

89A1-89A2

Sequence location

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89A1-89A2

Left limit from inclusion within Df(3R)Aldoxⁿ¹ (FBrf0042261) Right limit from inclusion within Df(3R)Aldoxⁿ¹ (FBrf0042261)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-56.6

(Rumball et al., 1994)

(Choudhary et al., 1992)

3-57.2

Left of (cM)

Right of (cM)

AOX1

(Nelson and Szauter, 1992)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4025

Men^nNC3 Aldox89A^nNC4/TM2

37751

se¹ ry¹ AOX1^lpo Aldox89Aⁿ¹ Sb^sbd-2

4068

Pgk² Pgi²/SM1, Pgk⁴ Pgi⁴; Men⁶ ry^el-S Aldox89A³ Mdh2⁶/TM6, Men⁴ ry^el-F Aldox89A^L4 Mdh2⁴

Kyoto

101572

Aldox89A^L6

101573

Aldox89A^nNC1 / TM6

101983

Gpdh1^B Mdh1^S Adh^F ; Fdh^F Aldox89A^S

101984

Gpdh1^B Mdh1^S Adh^S ; Fdh^S Aldox89A^S

107571

Pgk² Pgi²/SM1, Pgk⁴ Pgi⁴; Men⁶ ry^el-S Aldox89A³ Mdh2⁶/TM6, Men⁴ ry^el-F Aldox89A^L4 Mdh2⁴

103359

SM1, al² Duox^Cy cn² speck² Pgk⁴ Pgi⁴ / Pgk² Pgi² ; TM6, Hn^P ss^aP88 Ubx^bx-34e Ubx^P15 e¹ Men⁴ ry^el-F Aldox89A^L4 Mdh2^* / Men⁶ ry^el-S Aldox89A³ Mdh2^*

Genomic Clones (0)

cDNA Clones (0)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The effect of various concentrations of ethanol and long-term ethanol exposure on flies carrying unique allele combinations at the Fdh and Aldox-1 loci is studied.

(Bokor and Pecsenye, 1997)

The effect of environmental ethanol on larval to pupal survival and on the activities of Fdh, Aldox-1, Adh and Gpdh is investigated.

(Pecsenye et al., 1997)

Activity change of the enzyme due to the influence of ethanol, starvation and ethanol stress is assayed.

(Pecsenye et al., 1996)

Gramoxone has no mutagenic effect on the genetic background of Aldox-1.

(el Abidin Salam et al., 1993)

No difference in allele fixed in lines selected over 700 generations for high (negative) and low (positive) geotaxis.

(Stoltenberg et al., 1992)

The role of Adh, Aldh and Aldox-1 in alcohol/aldehyde metabolism in D.melanogaster has been studied.

(Anderson and Barnett, 1991)

Aldox-1 enzyme activity has been analysed in Abd-B^iab7-SGA/+ eye-antennal discs.

(Awad et al., 1981)

Structural gene for aldehyde oxidase. ALDOX activity increases nonuniformly during development with step increases at pupation and midway through pupal stage. First increase appears to be controlled by a closely linked cis-acting element (FBrf0027301) and the latter by Aldox-2⁺ (FBrf0033935). Control independent of that of the closely linked Po (FBrf0028397). Maternal enzyme present through to early larva, but replaced by zygotic gene products by late larva. Tissue specificity varies with stage and strain (FBrf0023907; FBrf0037508). Heptaldehyde serves as specific substrate (FBrf0037508). Differential staining for enzyme noted in different compartments of the wing imaginal disc (FBrf0028823).

Relationship to Other Genes

Source for database merge of

Additional comments

Based on analysis of the Aldox-1ⁿ¹ mutation, Aldox-1 likely corresponds to AOX2 and/or AOX3.

(Marelja et al., 2014)

Nomenclature History

Source for database identify of

Source for identity of: Aldox89A Aldox-1

(FlyBase, 2009-)

Nomenclature comments

Renamed this locus from 'Aldox-1' (fullname 'Aldehyde oxidase 1') to 'Aldox89A' (fullname 'Aldehyde oxidase at 89A') to better distinguish from the sequenced localized 'AOX1' (fullname 'Aldehyde oxidase 1') gene with which it shared the same full name.

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

(Bondinas et al., 2002, Bondinas et al., 2001, Choudhary et al., 1992, Schott et al., 1986, Bentley et al., 1981)

AOX

(Pecsenye et al., 1996)

Aldox

(Pecsenye and Saura, 1998, Bokor and Pecsenye, 1997, Hamblin and Aquadro, 1996, Pecsenye and Meglecz, 1995, Pecsenye and Tothmeresz, 1994, Pecsenye et al., 1994, Pecsenye et al., 1994, Rumball et al., 1994, Hastings, 1992, Anderson and Barnett, 1991, van Zijll Langhout and van Breugel, 1985, Burkhart et al., 1984, Browder et al., 1982, Regenass and Bernhard, 1978, Pipkin et al., 1976)

Aldox-1

Aldox89A

(Matthews and Munstermann, 1994, Parkash and Shamina, 1994, Parkash and Vandna, 1994, Shamina and Parkash, 1994, Shamina and Parkash, 1994, Zuzick and Dapkus, 1994, el Abidin Salam et al., 1993, Parkash et al., 1993)

Ao-1

(Zapata et al., 2002)

Aox

(Pecsenye et al., 1997)

aldox

(Awad et al., 1981)

lao

Name Synonyms

Aldehyde oxidase 1

Aldehyde oxidase at 89A

aldehyde oxidase-1

(Zapata et al., 2002)

low aldehyde oxidase

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 0 )

References (61)

NA

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