FlyBase Gene Report: Dmel\Ama

General Information

Symbol

Dmel\Ama

Species

D. melanogaster

Name

Amalgam

Annotation Symbol

CG2198

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000071

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Pathway
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

84A5-84A5

Sequence location

3R:6,762,593..6,765,261 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:6762593..6765261

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables receptor ligand activity

inferred from direct assay

(Fremion et al., 2000)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules

inferred from physical interaction with FLYBASE:Nrt; FB:FBgn0004108

(Fremion et al., 2000)

involved_in maintenance of blood-brain barrier

inferred from mutant phenotype

(Ariss et al., 2020)

involved_in positive regulation of epidermal growth factor receptor signaling pathway

inferred from genetic interaction with FLYBASE:Egfr; FB:FBgn0003731, FLYBASE:Pi3K92E; FB:FBgn0015279

(Ariss et al., 2020)

involved_in positive regulation of glial cell migration

inferred from mutant phenotype

(Ariss et al., 2020)

involved_in positive regulation of glial cell proliferation

inferred from mutant phenotype

(Ariss et al., 2020)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Fremion et al., 2000)

located_in plasma membrane

inferred from direct assay

(Fremion et al., 2000, Seeger et al., 1988)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Pathway (FlyBase)

Positive Regulators of EGFR Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Pathway (FlyBase)

Positive Regulators of EGFR Signaling Pathway -: Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Ama: Amalgam

Antibody staining first detects Amalgam in the mesoderm during gastrulation; as neuroblasts delaminate from the ectoderm staining appears in a row of mesectodermal cells along the ventral midline of the extended germ band. Amalgam appears in the first neurons generated from the ganglion-mother cells, but not in the neuroblast precursors of these cells. Neuronal accumulation of Ama gene product increases during CNS development, but appears to be confined to the CNS and initially does not extend to axons exiting the CNS in segmental, intersegmental, or peripheral nerves; with time three rows of PNS-associated cells accumulate Ama protein; staining heavy around spiracle sensory organ and several cephalic sensory structures. Simultaneously there is a complicated temporal and spatial sequence of staining of mesodermal derivatives. Embryonic phenotype of deletion of Ama attributable to simultaneous deletion of zen; no effect of Ama- detectable.

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ama for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ama-RA

FBtr0081619

NM_057231

1542

333

Ama-RB

FBtr0081618

NM_169160

1833

341

Ama-RC

FBtr0081620

NM_176412

1526

333

Additional Transcript Data and Comments

Reported size (kB)

1.5 (northern blot)

(Seeger et al., 1988)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ama-PA

FBpp0081136

36.4

333

7.04

NP_476579

AAF54084

Ama-PB

FBpp0081135

37.4

341

7.28

NP_731114

AAN13372

Ama-PC

FBpp0081137

36.4

333

7.04

NP_788589

AAO41515

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

333 aa isoforms: Ama-PA, Ama-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

333 (aa)

(Seeger et al., 1988)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ama using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ama_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

(Seeger et al., 1988)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

epithelial cell

• cytoplasm | apical

Comment: clustered apical distribution

(Lecuyer et al., 2007)

epithelium

• cytoplasm | apical

(Lecuyer et al., 2007)

embryonic stage 13 -- 15

embryonic midgut constriction 1

(Fremion et al., 2000)

embryonic/larval gut

Comment: diffuse localization

(Fremion et al., 2000)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Seeger et al., 1988)

Additional Descriptive Data

The Ama transcript has two major peaks of accumulation during development, one during mid-embryogenesis, and the second during early pupation.

(Seeger et al., 1988)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 10

amnioserosa

(Seeger et al., 1988)

embryonic stage 16

embryonic/larval dorsal vessel

(Seeger et al., 1988)

embryonic/larval labral segment sense organ | precursor

(Seeger et al., 1988)

embryonic stage 13

visceral mesoderm

(Seeger et al., 1988)

embryonic stage 13 -- 17

peripheral nervous system

(Seeger et al., 1988)

cell fractionation

Stage

Tissue/Position (including subcellular localization)

Reference

S2 cell-line

(Fremion et al., 2000)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16

fat body

(Seeger et al., 1988)

hypophysis

(Seeger et al., 1988)

embryonic stage 13

spiracular hair

(Seeger et al., 1988)

embryonic stage 8

mesoderm

(Seeger et al., 1988)

embryonic stage 10 -- 17

presumptive embryonic/larval central nervous system | presumptive

(Seeger et al., 1988)

embryonic stage 10

neuron

(Seeger et al., 1988)

embryonic stage 13 -- 15

embryonic/larval central nervous system

(Fremion et al., 2000)

embryonic/larval fat body

(Fremion et al., 2000)

external sensory organ

(Fremion et al., 2000)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Ama protein is secreted.

(Fremion et al., 2000)

Ama protein is first detected in stage 8 embryos in the mesoderm. It begins to accumulate in the midline at late stage 9/early stage 10 and is found in a subset of the mesectodermal cells. At stage 10, Ama protein is observed on cells of the aminoserosa, especially along the border with the dorsal epidermis. Neuronal accumulation is first observed in stage 10, and by stage 13, Ama protein is observed on all of the cell bodies and axons of the CNS. However, no staining of axons is seen after they exit the CNS. Ama protein continues to accumulate in mesodermal derivatives. It is found in the visceral mesoderm in stage 13 and in the fat body and dorsal vessel in stage 16. Ama protein accumulation is observed in a subset of PNS cells starting in stage 13 including the spiracular sensory organ. During stage 16, Ama protein also accumulates on several cephalic sensory structures including the dorsal and terminal organs, the epiphysis and the hypophysis.

(Seeger et al., 1988)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular space

inferred from direct assay

(Fremion et al., 2000)

located_in plasma membrane

inferred from direct assay

(Fremion et al., 2000, Seeger et al., 1988)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ama in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ama

View exonic expression by tissue for Dmel\Ama

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000071

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0000071

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2198-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2198

Flygut - An atlas of the Drosophila adult midgut

FBgn0000071

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Ama

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ama

Transgenic constructs containing regulatory region of Ama

Deletions and Duplications ( 6 )

Disrupted in

Df(3R)ama

(Fremion et al., 2000)

Df(3R)D830

(Fremion et al., 2000)

Df(3R)MAP11

(Liebl et al., 2003)

Df(3R)MAP117

(Kaufman et al., 1990)

Df(3R)SCB-XL2

(Kaufman, 1994.10.5)

Not disrupted in

Df(3R)MAP8

(Kaufman et al., 1990)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Ama^GD12733

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ama^GD12733

viable, with Scer\GAL4^Mef2.PR

Ama^GD12733

viable, with Scer\GAL4^tin.CΔ4

Ama^GD12733

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (22)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\LSAMP

8 of 15

Yes

7 of 15

6 of 15

6 of 15

5 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Hsap\LRIT3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (20)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Lsamp

8 of 15

Yes

7 of 15

7 of 15

6 of 15

6 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Mmus\Lrit3

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (18)

Rnor\Lsamp

7 of 13

Yes

6 of 13

6 of 13

6 of 13

5 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (16)

Xtro\iglon5

6 of 12

Yes

Xtro\lsamp

6 of 12

Yes

Xtro\negr1

6 of 12

Yes

Xtro\ntm

6 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\myot

1 of 12

Yes

Xtro\ncam2

1 of 12

Xtro\papln

1 of 12

Xtro\vsig10

1 of 12

Yes

Danio rerio (Zebrafish) (36)

Drer\ntm

6 of 15

Yes

5 of 15

5 of 15

5 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Drer\lrit1b

1 of 15

Yes

Drer\lrit3a

1 of 15

Yes

Drer\lrit3b

1 of 15

Drer\mdga1

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Drer\si:ch1073-459j12.1

1 of 15

Drer\si:dkeyp-75h12.2

1 of 15

Drer\ush2a

1 of 15

Yes

Drer\vcam1a

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (14)

Cele\rig-5

4 of 15

Yes

Cele\wrk-1

2 of 15

Cele\zig-3

2 of 15

Cele\C53A5.13

1 of 15

Yes

Cele\igcm-2

1 of 15

Cele\lad-2

1 of 15

Cele\let-805

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190BE6 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00011665

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00011664

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\Ama

Drosophila persimilis

Dper\GL21774

Drosophila willistoni

Dwil\GK12252

Drosophila virilis

Dvir\GJ23304

Drosophila mojavensis

Dmoj\GI22890

Drosophila grimshawi

Dgri\GH18131

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915073A )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17020767

Glossina morsitans

Tsetse fly

Gmor\GMOY009893

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_01938

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W069F )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011387

Danaus plexippus

Monarch butterfly

Dple\DPOGS211687-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL005375

Heliconius melpomene

Postman butterfly

Hmel\HMEL005441

Apis florea

Little honeybee

Aflo\3485492276

Apis mellifera

Western honey bee

Amel\GB49389

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP17400

Bombus terrestris

Buff-tailed bumblebee

Bter\15213246

Linepithema humile

Argentine ant

Lhum\LH16804

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002b06

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010514

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG013806

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG021848

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04671

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_15310

Pediculus humanus

Human body louse

Phum\PHUM287200

Rhodnius prolixus

Kissing bug

Rpro\RPRC002760

Cimex lectularius

Bed bug

Clec\CLEC008126

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008756

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007274

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004806

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000596

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_12663

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X064S )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008340

Strigamia maritima

European centipede

Smar\SMAR015125

Ixodes scapularis

Black-legged tick

Isca\ISCW003384

Stegodyphus mimosarum

African social velvet spider

Smim\KFM71118.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g01490

Tetranychus urticae

Two-spotted spider mite

Turt\tetur27g01990

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_212574

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G138W )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Negr1L

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Fn3/Igf_33

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Fn3/Igf_13

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hypp_2901

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Fn3/Igf_7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_013493gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hmcn1L_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hypp_1414

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (54)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

Lac

5 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

klg

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

bdl

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

rst

1 of 10

sdk

1 of 10

Strn-Mlck

1 of 10

tutl

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

LSAMP; limbic system associated membrane protein

8 of 15

603241

OPCML; opioid binding protein/cell adhesion molecule like

7 of 15

600632

OVARIAN CANCER

ovarian cancer

IGLON5; IgLON family member 5

6 of 15

618861

NTM; neurotrimin

6 of 15

607938

NEGR1; neuronal growth regulator 1

5 of 15

613173

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Ama - mir-124

quantitative reverse transcription pcr, luminiscence technology

(Sun et al., 2012)

protein-protein

Physical Interaction

Assay

References

Ama

molecular sieving, molecular weight estimation by staining, electron microscopy, circular dichroism, cosedimentation, enzyme linked immunosorbent assay

(Ozkan et al., 2013, Zeev-Ben-Mordehai et al., 2009)

Ama - Dlc90F

experimental knowledge based

(Guruharsha et al., 2011)

Ama - Nrt

bead aggregation assay, inferred by author, anti bait coimmunoprecipitation, western blot, fluorescence microscopy, affinity chromatography technology, Identification by mass spectrometry, pull down

(Zeev-Ben-Mordehai et al., 2009, Liebl et al., 2003, Fremion et al., 2000)

Ama - RluA-2

experimental knowledge based

(Guruharsha et al., 2011)

Ama - Wdfy2

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Abl

enhanceable

Ama

(Liebl et al., 2003)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66029

DroID - A comprehensive database of gene and protein interactions.

FBgn0000071

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40831

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Positive Regulators of EGFR Signaling Pathway -: Positive regulators of Epidermal Growth Factor Receptor signaling up-regulate the pathway, enhancing the activation of ERK kinase (rl) or acting on downstream effectors.

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84A5-84A5

Sequence location

3R:6,762,593..6,765,261 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84A5-84A5

Limits computationally determined from genome sequence between P{PZ}pb⁰⁴⁴⁹⁸ and P{lacW}l(3)L2100^L2100

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.2

(Comeron et al., 2012)

(Hill et al., 1995)

Left of (cM)

Diap1

(Liebl et al., 2003)

(Hill et al., 1995)

Right of (cM)

(Liebl et al., 2003, Hill et al., 1995)

Notes

3-47

(Liebl et al., 2003)

Stocks and Reagents

Stocks (7)

Bloomington

7439

w¹¹¹⁸; P{Hsp70-Ama}2

89905

Ama^M109/TM6B, Tb¹

90305

Abl¹ Ama^R1/TM6B, Tb¹

33416

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00297}attP2

Kyoto

103970

y^* w^*; P{GawB}Ama^NP1297 / TM6, P{UAS-lacZ.UW23-1}UW23-1

VDRC

v22944

w¹¹¹⁸ P{GD12733}v22944

v22945

w¹¹¹⁸; P{GD12733}v22945/TM3

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (41)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD39923

Other clones

DPiM_LD39923

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000071

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000071

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40831

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Seeger et al., 1988)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ama CG2198

(Li, 1999.11)

Source for database merge of

Source for merge of: Ama M109

(Liebl et al., 2001)

Additional comments

Other Comments

In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.

(Kliman and Eyre-Walker, 1998)

Nrt is hypothesized to require Ama as its ligand.

(Diano and Piovant, 1995)

Haploinsufficiency dependent upon an Abl mutant background (HDA).

(Hill et al., 1995)

The homologs of Antp, ftz, Scr, Dfd, Ama, bcd, zen, pb and lab, but not zen2 are all present in D.pseudoobscura.pseudoobscura, in the same linear order and similarly spaced along the chromosome as in D.melanogaster.

(Randazzo et al., 1993)

Encodes a protein of the immunoglobulin super-family that may function as a cell-adhesion protein.

(Lindsley and Zimm, 1992)

Embryonic phenotype of deletion of Ama attributable to simultaneous deletion of zen; no effect of Ama^- detectable.

(Lindsley and Zimm, 1992)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40831

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P15364

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000071

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000071

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Ama_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2198

Flygut - An atlas of the Drosophila adult midgut

FBgn0000071

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40831

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015310

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2198

modMine - A data warehouse for the modENCODE project

FBgn0000071

Linkouts

BioGRID - A database of protein and genetic interactions.

66029

DPiM - Drosophila Protein interaction map

FBgn0000071

DroID - A comprehensive database of gene and protein interactions.

FBgn0000071

DRSC - Results frm RNAi screens

FBgn0000071

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0000071

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2198-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000071

FlyMine - An integrated database for Drosophila genomics

FBgn0000071

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40831

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

AMA

(Pulido et al., 1992)

BG:DS00276.6

CG2198

(Herranz et al., 2012, Ni et al., 2010.12.1, Rhiner et al., 2010, Zeev-Ben-Mordehai et al., 2009, Klebes et al., 2005)

CT7244

(Hynes and Zhao, 2000)

M109

ama

(Liu et al., 2020, Liebl et al., 2003, Liebl et al., 2003, Forsthoefel et al., 2001, Liebl et al., 2001, Fremion et al., 2000, Denell, 1994, Duboule and Morata, 1994, Maroni, 1994, Karlstrom et al., 1993, Randazzo et al., 1993, Kaufman et al., 1990)

Name Synonyms

Amalgam

(Becker et al., 2016, Nguyen et al., 2016, Castillo et al., 2015, Smith et al., 2009, Zeev-Ben-Mordehai et al., 2009, Zeev-Ben-Mordehai et al., 2009, Gim et al., 2001)

amalgam

(Mudiganti et al., 2010, Rhiner et al., 2010, Jagla et al., 2001, Martin, 1999.1.14)

amalgam shotgun

(Liu et al., 2020)

Secondary FlyBase IDs

FBgn0015757

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (90)

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