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General Information
Symbol
Dmel\aop
Species
D. melanogaster
Name
anterior open
Annotation Symbol
CG3166
Feature Type
FlyBase ID
FBgn0000097
Gene Model Status
Stock Availability
Gene Snapshot
anterior open (aop) encodes a transcriptional repressor of the ETS family. It acts downstream of receptor tyrosine kinase signaling to regulate cell fate transitions critical to the development of many tissues including the nervous system, heart, trachea and eye. [Date last reviewed: 2019-03-07]
Also Known As
yan, pokkuri, pok, DROYANETSB, aop/yan
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:2,156,484..2,178,754 [-]
Recombination map
2-5
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
Belongs to the ETS family. (Q01842)
Summaries
Gene Group (FlyBase)
ETS DOMAIN TRANSCRIPTION FACTORS -
The E26 transformation specific (ETS) domain transcription factors are sequence-specific DNA-binding proteins that regulate transcription. These factors are characterized by a conserved 85 amino acid DNA binding ETS domain that binds specifically to purine-rich DNA motif GGAA/T. (Adapted from PMID:11715049 and PMID:9570133).
Pathway (FlyBase)
Sevenless Signaling Pathway Core Components -
The specification of the R7 photoreceptor cell in each ommatidium of the developing Drosophila eye is dependent on activation of Sevenless receptor tyrosine kinase, which acts via the canonical Ras/Raf/MAP kinase cascade to promote the expression of lz and pros. sev, expressed in presumptive R7 cells, is activated by binding to Bride of Sevenless (boss), a seven-transmembrane protein expressed in R8 cells. (Adapted from FBrf0127283 and FBrf0221727).
Fibroblast Growth Factor Receptor Signaling Pathway Core Components -
Fibroblast Growth Factor Receptor (FGFR) signaling pathway is initiated by the binding of secreted FGFs - bnl or ths/pyr to receptor tyrosine kinases btl or htl, respectively, to initiate signaling primarily via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0221038).
Epidermal Growth Factor Receptor Signaling Pathway Core Components -
The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components -
PDGF/VEGF-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of canonical Wnt signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Protein Function (UniProtKB)
Ets-related protein that functions as a negative regulator of photoreceptor development acting antagonistically to pnt and the proneural signal mediated by RAS (PubMed:1505027, PubMed:1495974, PubMed:8033205). It acts upstream of SINA to inhibit R7 development (PubMed:1505027, PubMed:1495974).
(UniProt, Q01842)
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
aop: anterior open
Embryonic lethal. Homozygous embryos have anterior dorsal hole in epidermis. Brain and sometimes gut extrude through hole. Head involution normal. Visible during dorsal closure.
Summary (Interactive Fly)
ets domain transcription factor - target of Ras pathway that serves to inhibit neural and other types of differentiation - crucial to the development of the nervous system, heart, trachea and eye
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\aop or the JBrowse view of Dmel\aop for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.44
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0077851
4266
732
FBtr0077850
4879
732
FBtr0330658
4936
732
FBtr0330659
4181
732
FBtr0330660
3936
732
Additional Transcript Data and Comments
Reported size (kB)
5.7, 4.8 (northern blot); 1.6 (compiled cDNA)
4.6 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0077523
78.6
732
7.87
FBpp0077522
78.6
732
7.87
FBpp0303508
78.6
732
7.87
FBpp0303509
78.6
732
7.87
FBpp0303510
78.6
732
7.87
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

732 aa isoforms: aop-PA, aop-PB, aop-PC, aop-PD, aop-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
761 (aa); 88 (kD observed); 85 (kD predicted)
Comments
There are numerous polymorphic differences between g158811 and g217342.
The aop amino acid sequences predicted in FBrf57549 and FBrf55572 differ substantially in three separate regions of the protein.
External Data
Post Translational Modification
Phosphorylated in response to MAPK signaling. May be phosphorylated by rl.
(UniProt, Q01842)
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\aop using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (38 terms)
Molecular Function (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000218930
(assigned by GO_Central )
Biological Process (27 terms)
Terms Based on Experimental Evidence (24 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:pnt; FB:FBgn0003118
inferred from genetic interaction with FLYBASE:foxo; FB:FBgn0038197
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
inferred from genetic interaction with FLYBASE:chico; FB:FBgn0024248
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by UniProt )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:rl; FB:FBgn0003256
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Ras85D; FB:FBgn0003205
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000218930
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000218930
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000218930
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
aop is expressed in lateral neuroepithelial cells and the medial part of the lamina in a pattern complementary to pnt.
aop expression is observed in the Bolwig organ primordium from embryonic stage 10/11 to stage 12/13 and is gone by stage 15.
aop protein is not detected in embryos until the germ band is fully extended. At stage 10, it is expressed in most regions of the epidermis. During germ band retraction, expression is gradually restricted. By stage 13, there are only 40-50 aop-positive cells that appear to correspond to most of the cells of the tracheal system. In third instar larvae, expression is observed in the eye disc but not in other discs. aop is expressed in most nuclei near the morphogenetic furrow, throughout the depth of the epithelium. Posterior to the furrow, only uncommitted cells express aop. As cells begin to differentiate, their nuclei rise in the epithelium and the level of aop rises dramatically. In pupal eye discs, aop protein is expressed in cone cells, in pigment cells, and in one cell of the bristle group.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from high throughput direct assay
inferred from high throughput direct assay
inferred from mutant phenotype
(assigned by UniProt )
inferred from high throughput direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\aop in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 11-12
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 72 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 33 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of aop
Transgenic constructs containing regulatory region of aop
Deletions and Duplications ( 6 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
adult abdomen & trichogen cell, with Scer\GAL4sca-537.4
macrochaeta & eye
sensory neuron & axon & embryo, with Scer\GAL4repo
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
10 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
Rattus norvegicus (Norway rat) (8)
10 of 13
Yes
Yes
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
1 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (5)
5 of 12
Yes
Yes
1 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (7)
7 of 15
Yes
Yes
2 of 15
No
Yes
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
1 of 15
Yes
No
1 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091905CC )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502WL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W054P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X050Z )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (8)
3 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    suppressible
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    enhanceable
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Gene Group - Pathway Membership (FlyBase)
    Sevenless Signaling Pathway Core Components -
    The specification of the R7 photoreceptor cell in each ommatidium of the developing Drosophila eye is dependent on activation of Sevenless receptor tyrosine kinase, which acts via the canonical Ras/Raf/MAP kinase cascade to promote the expression of lz and pros. sev, expressed in presumptive R7 cells, is activated by binding to Bride of Sevenless (boss), a seven-transmembrane protein expressed in R8 cells. (Adapted from FBrf0127283 and FBrf0221727).
    Fibroblast Growth Factor Receptor Signaling Pathway Core Components -
    Fibroblast Growth Factor Receptor (FGFR) signaling pathway is initiated by the binding of secreted FGFs - bnl or ths/pyr to receptor tyrosine kinases btl or htl, respectively, to initiate signaling primarily via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0221038).
    Epidermal Growth Factor Receptor Signaling Pathway Core Components -
    The Epidermal Growth Factor Receptor (EGFR) signaling pathway is used multiple times during development (FBrf0190321). It is activated by the binding of a secreted ligand to the receptor tyrosine kinase Egfr and acts via the canonical Ras/Raf/MAP kinase (ERK) cascade. (Adapted from FBrf0190321 and FBrf0221727).
    Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components -
    PDGF/VEGF-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependent manner. (Adapted from FBrf0222697 and FBrf0221727).
    Negative Regulators of Wnt-TCF Signaling Pathway -
    Negative regulators of canonical Wnt signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-5
    Cytogenetic map
    Sequence location
    2L:2,156,484..2,178,754 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    22D1-22D1
    Limits computationally determined from genome sequence between P{lacW}CG31672k09932 and P{PZ}aop03953a&P{EP}aopEP2500
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    22D-22D
    (determined by in situ hybridisation)
    22D1-22D2
    (determined by in situ hybridisation)
    22C-22C
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Notes
    Stocks and Reagents
    Stocks (44)
    Genomic Clones (16)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (70)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
      Commercially Available Antibodies
       
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: aop CG3166
      Source for database merge of
      Source for merge of: aop yan pok
      Additional comments
      Other Comments
      aop is dispensible for primary tracheal branching, but plays a key role in tracheal tip cell fate specification, where it acts to inhibit both terminal cell and fusion cell specification, by inhibiting MAPK and Wingless signaling respectively.
      dsRNA has been made from templates generated with primers directed against this gene. RNAi of aop results in increased arborization of ddaD and ddaE neurons. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
      There is reciprocal negative regulation between mir-7 and aop in retinal cells.
      SL2 cells transfected with dsRNA made from templates generated with primers directed against this gene show decreased levels of endocytosis.
      Monomeric aop is exported from the nucleus in a emb-dependent manner.
      RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
      RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
      aop serves as a common target of N/Su(H) and RTK/pnt signalling pathways during cell fate specification.
      Phosphorylation by rl of aop depends on edl, and is mediated by the binding of aop to edl protein through their Pointed domains.
      260 enhancers and 90 suppressors have been identified in a screen for genes functioning downstream in the Ras/MAPK/yan pathway, by virtue of interacting with alleles of aop.
      Genetic studies suggest that 14-3-3ε functions in multiple receptor tyrosine kinase pathways, acting downstream or parallel to phl, but upstream of aop and phyl, two nuclear factors involved in Ras85D signalling.
      Mutant phenotype indicates both aop and ttk are involved in the Ras/MAPK pathway, although their mechanisms of action to inhibit to inhibit cell fate might be different. aop and ttk synergistically interact in an inhibitory signaling pathway that is critical for neural cell fate determination.
      aop and ttk mutations dominantly suppress eye phenotypes of ksr mutants.
      Shows no genetic interaction with sdk.
      Transcription factors Jra and aop are required for dorsal closure. Results suggest that the bsk pathway governs dorsal closure at least partially by regulating dpp expression via phosphorylation of Jra and aop.
      aop antagonises pntP2 function in the midline glial cells.
      The proper induction of Egfr genes requires the concomitant inactivation of aop, mediated by Egfr signalling.
      pros gene becomes transcriptionally activated at a low level in all sev-competent cells prior to sev signaling and this requires the activities of Ras85D and two ETS transcription factors, aop and pnt.
      aop and ttk mutations both act to repress inappropriate R7 cell determination, their mechanisms of action differ.
      In aop mutants the presence of supernumerary R7 cells depends on sina activity.
      Genetic data suggest phyl acts downstream of Ras85D, phl and aop to promote neuronal differentiation in R1, R6 and R7.
      Mutations of aop have no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.
      Map kinase mediated down-regulation of aop function appears to be critical for the proper differentiation of both neuronal and nonneuronal tissues throughout development. This suggests that aop is an essential component of a general timing mechanism controlling the competence of a cell to respond to inductive signals.
      Phosphorylation of aop by rl map kinase affects the stability and subcellular localisation of aop resulting in rapid down regulation of aop activity. Expression of mutant aop in Schneider S2 cells demonstrates that the first phosphorylation site is absolutely required in the response to activation of the Ras85D/rl pathway, phosphorylation at other sites is important for modulation or amplification of the response.
      aop acts as a transcriptional repressor that is itself negatively regulated by the Ras85D signal.
      aop has a role in the specification of the fate of the cell that develops into R7 from the true R7 precursor. Mosaic analysis suggests the function of the aop gene product is autonomously required for the development of a cell as a photoreceptor neuron. The complete loss of aop during development does not give rise to an adult retina with extra photoreceptor neurons.
      A screen to identify mutations affecting the Ras85D signalling pathway identified alleles of phl, Dsor1, rl, aop, βggt-I, mts, ksr and phyl.
      The interaction between Jra and pnt is antagonized by aop.
      MAP kinase activity, encoded by the rl locus, induces neuronal differentiation by simultaneously inhibiting the aop repressor and stimulating the pnt activator.
      aop functions as a cell-autonomous negative regulator of photoreceptor development. In the presumptive R7 and cone cells aop appears to act synergistically to the proneural signal mediated by sev and Ras85D.
      Receptor cell R7 can be produced in the absence of sev+ by disrupting aop.
      Mosaic analysis demonstrates that no photoreceptor cell absolutely requires aop for proper ommatidial development.
      aop encodes an Ets domain protein that appears to interact with the transduction cascade downstream of sev in the eye.
      Origin and Etymology
      Discoverer
      Etymology
      'yan' is the name of a Chinese literary character with three eyes.
      'pokkuri' is a Japanese word that means 'dropping dead'.
      Identification
      External Crossreferences and Linkouts ( 82 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      KEGG Genes - Molecular building blocks of life in the genomic space.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Linkouts
      ApoDroso - Functional genomic database for photoreceptor development, survival and function
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (21)
      Reported As
      Symbol Synonym
      yan
      (Boisclair Lachance et al., 2018, Du et al., 2018, Hope et al., 2018, Webber et al., 2018, Feng et al., 2017, Jin et al., 2015, Xia et al., 2015, Boisclair Lachance et al., 2014, Manansala et al., 2013, Shwartz et al., 2013, Webber et al., 2013, Webber et al., 2013, Technau et al., 2012, Weber et al., 2012, Halfon et al., 2011, Kim et al., 2011, Olson et al., 2011, Tokusumi et al., 2011, Weyers et al., 2011, Figeac et al., 2010, Flynt et al., 2010, Salzer et al., 2010, Zhang et al., 2010, Corl et al., 2009, Krejcí et al., 2009, Li et al., 2009, Chang et al., 2008, Roukens et al., 2008, Wang et al., 2008, Weber et al., 2008, Letizia et al., 2007, Zeitlinger et al., 2007, Arbouzova et al., 2006, Baril and Therrien, 2006, Liu et al., 2006, Apitz et al., 2005, Charroux et al., 2005, Dutta et al., 2005, Stern et al., 2005, Vivekanand et al., 2005, Baril and Therrien, 2004, Bateman and McNeill, 2004, Brodu et al., 2004, Doroquez et al., 2004, Gallio et al., 2004, Pollock et al., 2004, Vivekanand et al., 2004, zur Lage et al., 2004, Bidet et al., 2003, Morey et al., 2003, Ramos et al., 2003, Shilo, 2003, Tootle et al., 2003, Williams et al., 2003, Baonza et al., 2002, Behan et al., 2002, Halfon et al., 2002, Lai, 2002, Rohrbaugh et al., 2002, Rohrbaugh et al., 2002, Bai et al., 2001, Baker et al., 2001, Deshpande and Urban, 2001, Rohrbaugh et al., 2001, Silver et al., 2001, Tang et al., 2001, Tootle and Rebay, 2001, Abdelilah-Seyfried et al., 2000, Baker, 2000, Bui et al., 2000, Canon and Banerjee, 2000, Cox et al., 2000, Fanto, 2000, Fanto et al., 2000, Firth et al., 2000, Flores et al., 2000, Halfon et al., 2000, Hsu and Schulz, 2000, Kuang et al., 2000, Kumar and Moses, 2000, Raabe, 2000, Ramos et al., 2000, Rebay et al., 2000, Simon, 2000, Smith et al., 2000, Therrien et al., 2000, Xu et al., 2000, Bai et al., 1999, Bonini and Fortini, 1999, Flores and Engels, 1999, Golembo et al., 1999, Kramer et al., 1999, McNeill and Downward, 1999, Price and Lai, 1999, Staehling-Hampton et al., 1999, Warrick et al., 1999, Wessells et al., 1999, Wittwer et al., 1999, Dickson, 1998, Dumstrei et al., 1998, Hacohen et al., 1998, Hayashi et al., 1998, Ip and Davis, 1998, Kurada and White, 1998, Maixner et al., 1998, Meier and Evan, 1998, Price et al., 1998, Rorth et al., 1998, Stocker and Hafen, 1998, Blaumueller and Mlodzik, 1997, Carthew et al., 1997, Chang and Rubin, 1997, Isaksson et al., 1997, Kramer et al., 1997, Kumar and Moses, 1997, Lai et al., 1997, Lai et al., 1997, Li and Perrimon, 1997, Nguyen et al., 1997, Okabe and Okano, 1997, Perrimon and Perkins, 1997, Price et al., 1997, Rubin et al., 1997, Scholz et al., 1997, Scholz et al., 1997, Schweitzer and Shilo, 1997, Tang et al., 1997, Badenhorst et al., 1996, Dickson et al., 1996, Dominguez and Hafen, 1996, Gabay et al., 1996, Huang and Fischer-Vize, 1996, Karim et al., 1996, Karlin and Burge, 1996, Kauffmann et al., 1996, Lai et al., 1996, Treisman, 1996, Begemann et al., 1995, Brand and Dormand, 1995, Chang et al., 1995, Dickson, 1995, Dickson et al., 1995, Goldstein and Clark, 1995, Hafen et al., 1995, Hariharan et al., 1995, Lai et al., 1995, Rebay and Rubin, 1995, Rebay et al., 1995, Rogge et al., 1995, Therrien et al., 1995, Treier et al., 1995, Treisman et al., 1995, Anonymous, 1994, Brown and Hartley, 1994, Chang et al., 1994, Chang et al., 1994, Freeman, 1994, Kramer and Cagan, 1994, O'Neill et al., 1994, Perrimon and Desplan, 1994, Simon, 1994, Yamamoto, 1994, Freeman, 1993, Manning and Krasnow, 1993, Lai and Rubin, 1992, Lai and Rubin, 1991)
      Secondary FlyBase IDs
      • FBgn0003123
      • FBgn0005623
      • FBgn0026827
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (422)