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FB2025_02
,
released April 17, 2025
yan, pokkuri, pok, DROYANETSB, aop/yan
ets domain transcription factor - target of Ras pathway that serves to inhibit neural and other types of differentiation - crucial to the development of the nervous system, heart, trachea and eye
Please see the JBrowse view of Dmel\aop for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.44
Gene model reviewed during 5.47
5.7, 4.8 (northern blot); 1.6 (compiled cDNA)
4.6 (northern blot)
732 (aa)
761 (aa); 88 (kD observed); 85 (kD predicted)
There are numerous polymorphic differences between g158811 and g217342.
The aop amino acid sequences predicted in FBrf57549 and FBrf55572 differ substantially in three separate regions of the protein.
Phosphorylated in response to MAPK signaling. May be phosphorylated by rl.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\aop using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: NOT ventral midline
aop is detectable in all tracheal nuclei, except in the terminal tracheal cells of each branch.
aop expression is observed in the Bolwig organ primordium from embryonic stage 10/11 to stage 12/13 and is gone by stage 15.
aop protein is not detected in embryos until the germ band is fully extended. At stage 10, it is expressed in most regions of the epidermis. During germ band retraction, expression is gradually restricted. By stage 13, there are only 40-50 aop-positive cells that appear to correspond to most of the cells of the tracheal system. In third instar larvae, expression is observed in the eye disc but not in other discs. aop is expressed in most nuclei near the morphogenetic furrow, throughout the depth of the epithelium. Posterior to the furrow, only uncommitted cells express aop. As cells begin to differentiate, their nuclei rise in the epithelium and the level of aop rises dramatically. In pupal eye discs, aop protein is expressed in cone cells, in pigment cells, and in one cell of the bristle group.
JBrowse - Visual display of RNA-Seq signals
View Dmel\aop in JBrowse
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
aop is dispensible for primary tracheal branching, but plays a key role in tracheal tip cell fate specification, where it acts to inhibit both terminal cell and fusion cell specification, by inhibiting MAPK and Wingless signaling respectively.
dsRNA has been made from templates generated with primers directed against this gene. RNAi of aop results in increased arborization of ddaD and ddaE neurons. RNAi also causes defects in muscle, alterations in the number of MD neurons, defects in dendrite morphogenesis and reproducible defects in da dendrite development.
SL2 cells transfected with dsRNA made from templates generated with primers directed against this gene show decreased levels of endocytosis.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
260 enhancers and 90 suppressors have been identified in a screen for genes functioning downstream in the Ras/MAPK/yan pathway, by virtue of interacting with alleles of aop.
Shows no genetic interaction with sdk.
Mutations of aop have no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.
Map kinase mediated down-regulation of aop function appears to be critical for the proper differentiation of both neuronal and nonneuronal tissues throughout development. This suggests that aop is an essential component of a general timing mechanism controlling the competence of a cell to respond to inductive signals.
Phosphorylation of aop by rl map kinase affects the stability and subcellular localisation of aop resulting in rapid down regulation of aop activity. Expression of mutant aop in Schneider S2 cells demonstrates that the first phosphorylation site is absolutely required in the response to activation of the Ras85D/rl pathway, phosphorylation at other sites is important for modulation or amplification of the response.
aop has a role in the specification of the fate of the cell that develops into R7 from the true R7 precursor. Mosaic analysis suggests the function of the aop gene product is autonomously required for the development of a cell as a photoreceptor neuron. The complete loss of aop during development does not give rise to an adult retina with extra photoreceptor neurons.
Mosaic analysis demonstrates that no photoreceptor cell absolutely requires aop for proper ommatidial development.
Source for merge of: aop yan pok
Source for identity of: aop CG3166
'yan' is the name of a Chinese literary character with three eyes.
'pokkuri' is a Japanese word that means 'dropping dead'.
