T8, EG:165H7.2 , T1a, AS-C T8
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Supported by strand-specific RNA-Seq data.
Gene model reviewed during 5.51
2.8 (northern blot)
1.6 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
396 (aa); 43.5 (kD)
Efficient DNA binding requires dimerization with another bHLH protein.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ase using the Feature Mapper tool.
Comment: reported as dorsal/lateral sensory complexes
ase is expressed in proneural cells along the ventral nerve cord from embryonic stage 8.
Expression assayed at stages 9, 11, 13, and 17. Expression may be continuous between assayed stages in some tissues.
ase expression is first detected shortly after gastrulation commences in single cells that are in the process of segregating from the neurectoderm. ase transcripts are expressed only in the neuroblast and not in other cells of the proneural clusters. The ase-expressing cells give rise to the entire first wave of segregating neuroblasts and most likely all of the second and third wave neuroblasts as well. ase transcripts are also expressed in at least some ganglion mother cells. After germ band retraction, they are found in cells in the ventral nerve cord and brain that are thought to correspond to larval neuroblasts and optic lobe anlage. In the PNS, ase transcripts are expressed in all the sense organ precursors (SOPs) and their progeny. From stage 10 onwards, ase transcript expression is also observed in the midgut anlage. In the wing disc, ase transcripts are expressed in all SOPS that arise at least up until 3hrs apf. ase is only expressed in one cell within each proneural cluster that will become the SOP. It is also expressed in SOPs in leg and antennal discs. In larval brain, ase transcripts are detected in CNS neuroblasts and in some of their progeny and in horseshoe-shaped group of cells in the optic lobe anlage. They are also detected in the eye disc.
In third instar larvae, ase is expressed in all imaginal discs in single cells that correspond to sensory organ mother cells (SMCs). It is also expressed in each of their two daughter cells (second order precursors) but is not detected in the final descendents of the SMC. It is also expressed in the descendents of the imaginal neuroblasts, the ganglion mother cells. Sites of ase expression other than proneural clusters are two stripes of cells at both sides of the presumptive anterior wing margin in the wing disc, and a broad stripe of cells posterior to the morphogenetic furrow in the eye-antennal disc.
ase transcripts are detected in neuroblasts and their progeny. Expression peaks at stages 10-11 of embryogenesis. ase is then detected in the CNS, the labrum, the optic lobe anlage, the procephalic neurogenic region, and the posterior midgut anlage. It is also detected in PNS precursor cells. In third instar larvae, expression is detected in imaginal discs, CNS and optic lobe.
ase transcripts are first detected in late stage 8 embyros and peak in stages 10 and 11. They are expressed in presumptive neural precursors once they have segregated from the ectoderm and are expressed with each wave of neuroblast segregation. Expression is also described in the primordia of the stomatogastric and optic lobes nervous systems, in the posterior midgut, in procephalic regions, and in a segmentally reiterating pattern probably in precursors to peripheral nervous system cells.
ase is detectable at the posterior edge of the outer optic anlage (outer proliferating center) as well as cells in the inner optic anlage (inner proliferating center. ase expression reaches a maximum at the posterior border of the OPC. Low levels of ase expression appear to be present at the lamina furrow.
ase expression is first detected shortly after gastrulation commences in the nuclei of single cells that are in the process of segregating from the neurectoderm. ase protein is expressed only in the neuroblast and not in other cells of the proneural clusters. The ase-expressing cells give rise to the entire first wave of segregating neuroblasts and most likely all of the second and third wave neuroblasts as well. ase protein is also expressed in at least some ganglion mother cells. After germ band retraction, it is found in cells in the ventral nerve cord and brain that are thought to correspond to larval neuroblasts and optic lobe anlage. In the PNS, ase protein is expressed in all the sense organ precursors (SOPs) and their progeny. From stage 10 onwards, ase protein expression is also observed in the midgut anlage. In the wing disc, ase protein is expressed in all SOPS that arise at least up until 3hrs apf. ase is only expressed in one cell within each proneural cluster that will become the SOP. It is also expressed in SOPs in leg and antennal discs. In larval brain, ase protein is detected in CNS neuroblasts and in some of their progeny and in horseshoe-shaped group of cells in the optic lobe anlage.
GBrowse - Visual display of RNA-Seq signals
View Dmel\ase in GBrowse 2
1-0
1-0.0
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: ase CG3258
All proneural proteins are similarly able to promote the segregation of a neural precursor at the MP2 neuroblast position but show distinct capacities in its specification.
Loss of function mutations in the AS-C lead to a significant reduction in sensory bristles and glial cells.
ase may be a neural precursor gene, rather than a proneural gene. Its products are found in the neural precursor during its formation but not in the proneural cluster of cells that gave rise to the neural precursor cell. Its expression persists longer than that of other proneural genes. The expression of ase is apparently downstream of the proneural and neurogenic genes, and its ectopic expression bypasses the requirement for ac and sc in the formation of the imaginal sense organs.
Defects due to ase mutations are enhanced by the haploid condition of other genes of the ASC, and can be corrected by an ase transgene. ase has a proneural function that participates in the singling out of the sensory mother cells that give rise to the recurved bristles of the anterior wing margin, and ectopically expressed ase generates extra sense organs.
The ase transcription unit shows high homology to the basic domain of the ac, sc and l(1)sc genes as revealed by cross-hybridization studies. The initiation of ase transcription is shifted temporally and spatially in relation to the other genes of the ASC, this implies that the ase promoter responds to specific positional cues for its expression.
The achaete-scute complex defines the basic topology of the sense organ pattern, rather than the type or precise location of the elements. ase is sufficient for the development of Class C neurons: a set of segmentally repeated multi-innervated structures and several mono-innervated sense organs. The ase function can to some extent be substituted by pcl.
