Gene model reviewed during 5.44
Gene model reviewed during 5.49
2.0 (northern blot)
None of the polypeptides share 100% sequence identity.
Core component of several methyltransferase-containing complexes. Component of the SET1 complex, composed at least of the catalytic subunit Set1, wds/WDR5, Wdr82, Rbbp5, ash2, Cfp1/CXXC1, hcf and Dpy-30L1. Component of the MLL3/4 complex composed at least of the catalytic subunit trr, ash2, Rbbp5, Dpy-30L1, wds, hcf, ptip, Pa1, Utx, Lpt and Ncoa6. Interacts with hcf, sktl and trr.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ash2 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ash2 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
DNA-protein interactions: genome-wide binding profile assayed for ash2 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
Phenotypes of ash2 mutants that survive to the end of metamorphosis reveal that the ash2 gene product does not solely regulate homeotic selector genes. Pattern formation abnormalities are observed on mutant legs and wings. ash2 is a nuclear protein that is present in larval imaginal discs at a low level and at a much higher level in imaginal discs after puparium formation. The size of the ash2 protein changes dramatically at the end of the third larval instar suggesting that the protein has different functions in larvae than in pupae.
ash1 and ash2 are trans-regulatory elements of homeotic selector gene regulation. ash2 is required for the functioning of the Ubx ABX/BX enhancer element, regulation involves different cis-regulatory regions that than of ash1.
Genetic tests were used to confirm that ash1 and ash2 belong to a functionally related class of genes, mutations in which cause a wide variety of homeotic transformations that are similar to the transformations caused by trx. Double heterozygotes of ash2 alleles and Df(3R)red-P93 show a significant penetrance of homeotic transformations.
A member of the trithorax group of genes.
Most alleles homozygous lethal; stage of lethality variable. Surviving and pharate adults display transformations similar to those indicated for ash1. Homozygous lethal; labial and wing discs appear normal; eye-antenna, prothoracic, haltere, leg and genital discs homeotically transformed. Disc and cell autonomous.