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Gene Dmel\ash2

General Information
Symbol	Dmel\ash2	Species	D. melanogaster
Name	absent, small, or homeotic discs 2	Annotation symbol	CG6677
Feature type	protein_coding_gene	FlyBase ID	FBgn0000139
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	ash-2
Genomic Location
Chromosome (arm)	3R	Recombination map	3-78.6
Cytogenetic map	96A13-96A13	Sequence location	3R:20,475,847..20,479,098 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene absent, small, or homeotic discs 2 is referred to in FlyBase by the symbol Dmel\ash2 (CG6677, FBgn0000139). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: zinc ion binding. There is experimental evidence that it is involved in the biological process: histone H3-K4 methylation; imaginal disc-derived wing vein specification; chromatin-mediated maintenance of transcription; imaginal disc-derived wing morphogenesis. 31 alleles are reported. The phenotypes of these alleles are annotated with: adult segment; organ system subdivision; adult mesothoracic segment; thoracic segment; imaginal precursor; appendage segment; dorsal thoracic disc; antennal segment; mouthpart; multi-cell-component structure. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: B30.2/SPRY domain; Concanavalin A-like lectin/glucanases superfamily; SPla/RYanodine receptor SPRY; SPla/RYanodine receptor subgroup; Zinc finger, FYVE/PHD-type; Zinc finger, PHD-type; Zinc finger, PHD-type, conserved site; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1635128_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of ash2. Gene sequence location is 3R:20475847..20479098.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	ash2 Most alleles homozygous lethal; stage of lethality variable. Surviving and pharate adults display transformations similar to those indicated for ash1. mad: many abnormal discs (A. Shearn) Homozygous lethal; labial and wing discs appear normal; eye-antenna, prothoracic, haltere, leg, and genital discs homeotically transformed. Disc and cell autonomous.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96A13-96A13   Limits computationally determined from genome sequence between P{PZ}crb07207&P{PZ}BRWD305842 and P{EP}CycB3EP3127
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96A17-96A19     (Adamsom, 1996.10.9) 96A11-96A16   (determined by in situ hybridisation)   (Deak et al., 1997)
Experimentally Determined Recombination Data
Location	3-78.6
Left of (cM)
Right of (cM)	aor
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ash2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ash2-RB FBtr0084659  NM_170160   1413   350 ash2-RC FBtr0084660  NM_176558   1949   556 ash2-RE FBtr0306171  NM_001260363   1271   220
Additional Transcript Data & Comments
Reported size (kB)	2.0 (northern blot) (Adamson and Shearn, 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ash2-PB   FBpp0084039   39.7   350   6.76     NP_733024   AAN14010 ash2-PC   FBpp0084040   63.2   556   6.92     NP_788735   AAO41602 ash2-PE   FBpp0297301   25.2   220   6.24     NP_001247292   AFH06609
Additional Polypeptide Data & Comments
Reported size (kDa)	572 (aa); 64.8 (kD predicted) (Adamson and Shearn, 1996) 94 (kD observed) (Papoulas et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • SPla/RYanodine receptor subgroup (IPR018355) B30.2/SPRY domain (IPR001870) Zinc finger, PHD-type (IPR001965) SPla/RYanodine receptor SPRY (IPR003877) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Concanavalin A-like lectin/glucanases superfamily (IPR008985) Zinc finger, RING/FYVE/PHD-type (IPR013083)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA950035   AA951292   AA951981   AE014297 AAN14010   AE014297 AAO41602   AE014297 AFH06609   AI456085   AI544034   AI946074   AW943098   AY051803 AAK93227   AZ300978   BF493771   BF496515   BG639175   BI163525   BI164105   BI168646   BI169126   BI236091   BI365102   BI373604   BI482746   BI633838   BT011328   BT120264   CO177919   CO191594   CO274437   CO308429   CO325795   CZ471498   CZ490398   EC062421   EC066257   EC212319   EC243961   EV576781   EV583820   EV584627   EV590097   GH748044   GH748612   GH753300   GH753858   GH754845   GH755987   GH756339   GH756342   GH757248   GH757720   GH758950   GH760092   GH761004   GH771668   GH771856   GH772563   GH774440   GH776749   GH777013   GH777628   GH778816   GH778961   GH780341   GH781533   GH781634   GH782137   GH782950   GH783513   GH783613   GH784755   GH785902   GH786498   GH787586   GH788831   GH789530   GH789672   GH789765   GH790522   GH790777   GH792248   GH794867   GH797337   GH799193   GH801621   GH802675   GH804805   GH807294   GH807691   GH808447   GH809329   GH810147   GH811616   GH812224   GH814832   GH815335   GH815367   GH820581   GH823202   GH823738   GH824343   GH824362   GH827527   GH831882   GH833068   GH833177   GH834762   GH834774   GH834904   GH835283   GH836805   GH837082   GH838137   GH838943   GH839537   GH839832   GH841681   GH841805   GH842605   GH843579   GH843891   GH844613   GH845768   GH846402   GH846774   GH847068   GH847691   GH850052   GH850266   GH850497   GH850521   GH851322   GH851407   GH854344   GH857848   GH857986   GH862013   GH863458   GH863982   GH864267   GH867639   GH867892   GH869287   GH869662   GH870199   GH871294   GH871859   GH872849   GH877571   GH878674   GH880191   GH880586   GH881965   GH882180   GH884790   GH885265   GH885463   GH886370   GH887041   GH887411   GH888406   GH888795   GH889624   GH890238   GH890550   GH890644   GH891088   GH891813   GH892674   GH896366   GH896532   GH897310   GH897703   GH899674   GH903617   GH904474   GH906405   GH907072   GH907336   GH908637   GH908869   GH910729   GH912797   GH914975   GH915870   GH916316   GH917572   GH918027   GH918785   GH919151   GH920488   GH920754   GH922703   GH923518   GH925058   GH925879   GH926148   GH926588   GH926973   GH929970   GH930320   GH932811   GH933124   GH935358   GH935487   GH937665   U73809 AAC47328
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IMW1 Q94545 Q9VC55
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Adamson and Shearn, 1996)
Additional Descriptive Data
	The ash2 transcript is present throughout development. (Adamson and Shearn, 1996)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference larval stage | late   embryonic/larval fat body   (Adamson and Shearn, 1996) imaginal disc   (Adamson and Shearn, 1996) larval stage -- pupal stage     (Adamson and Shearn, 1996) third instar larval stage   embryonic/larval salivary gland   (Adamson and Shearn, 1996)
Additional Descriptive Data
	In Western blots, a 70 kD protein is detected in 2nd and 3rd instar larvae and a 53 kD protein is detected in pupae. The ash2 protein is detected in imaginal discs and in the nuclei of salivary gland and fat body cells. (Adamson and Shearn, 1996)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
MLL1/2 complex inferred from direct assay (Mohan et al., 2011) MLL3/4 complex inferred from direct assay (Mohan et al., 2011) nucleus inferred from direct assay AND inferred from sequence or structural similarity (Adamson and Shearn, 1996) polytene chromosome inferred from direct assay (Beltran et al., 2007) Set1C/COMPASS complex inferred from direct assay (Ardehali et al., 2011, Hallson et al., 2012, Mohan et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    225.175 Larval Midgut    78.4 Larval Hindgut    164.8 Larval Malpighian Tubules    71.1 Larval Fat Body    128.5 Larval Salivary Gland    101.1 Larval Trachea    293.825 Larval Carcass    98.325 Adult Head    215.6 Adult Eye    293.35 Adult Brain    136.7 Adult Thoracic-Abdominal Ganglion    167.6 Adult Crop    296.2 Adult Midgut    146.1 Adult Hindgut    200.7 Adult Malpighian Tubules    127.9 Adult Fat Body    269.8 Adult Salivary Gland    133.3 Adult Heart    200.125 Adult VirginFemale Spermatheca    170.9 Adult InseminatedFemale Spermatheca    162.6 Adult Ovary    167.8 Adult Testis    73.2 Adult Male Accessory Gland    257.4 Adult Carcass    209.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    215.6   NA  Eye    293.35   NA  Brain    136.7   225.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    167.6   NA  Crop    296.2   78.4  Midgut    146.1   164.8  Hindgut    200.7   71.1  Malpighian Tubules    127.9   128.5  Fat Body    269.8   101.1  Salivary Gland    133.3   NA  Heart    200.125   293.825  Trachea    NA   NA  VirginFemale Spermatheca    170.9   NA  InseminatedFemale Spermatheca    162.6   NA  Ovary    167.8   NA  Testis    73.2   NA  Male Accessory Gland    257.4   98.325  Carcass    209.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    14 central nervous system, pupae P8    5 head, virgin 1-day female    3 head, virgin 4-day female    4 head, virgin 20-day female    8 head, mated 1-day female    4 head, mated 4-day female    4 head, mated 20-day female    6 head, mated 1-day male    5 head, mated 4-day male    5 head, mated 20-day male    9 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    4 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    8 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    18 ovary, mated 4-day female    16 testis, mated 4-day male    8 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    53 embryo 04-06hr    43 embryo 06-08hr    27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr  (53) embryo 04-06hr  (43) embryo 06-08hr  (27) embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    53 embryo 04-06hr    43 embryo 06-08hr    27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    53 embryo 04-06hr    43 embryo 06-08hr    27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    53 embryo 04-06hr    43 embryo 06-08hr    27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr  (53) embryo 04-06hr  (43) embryo 06-08hr  27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    53 embryo 04-06hr    43 embryo 06-08hr    27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    53 embryo 04-06hr    43 embryo 06-08hr    27 embryo 08-10hr    25 embryo 10-12hr    16 embryo 12-14hr    12 embryo 14-16hr    15 embryo 16-18hr    12 embryo 18-20hr    7 embryo 20-22hr    19 embryo 22-24hr    17 larva L1    10 larva L2    9 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    14 larva L3 puffstage 7-9    15 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    22 pupae 2d postWPP    15 pupae 3d postWPP    9 pupae 4d postWPP    7 adult male 01day    11 adult male 05day    14 adult male 30day    14 adult female 01day    20 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    19 embryonic 1182-4H    30 embryonic GM2    18 embryonic Kc167    18 embryonic S1    97 embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS    30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4    19 embryonic 1182-4H  (30) embryonic GM2    18 embryonic Kc167    18 embryonic S1  (97) embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32  26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS  (30) ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    19 embryonic 1182-4H    30 embryonic GM2    18 embryonic Kc167    18 embryonic S1    97 embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS    30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    19 embryonic 1182-4H    30 embryonic GM2    18 embryonic Kc167    18 embryonic S1    97 embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS    30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    19 embryonic 1182-4H    30 embryonic GM2    18 embryonic Kc167    18 embryonic S1    97 embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS    30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4    19 embryonic 1182-4H  30 embryonic GM2    18 embryonic Kc167    18 embryonic S1  (97) embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32  26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS  30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    19 embryonic 1182-4H    30 embryonic GM2    18 embryonic Kc167    18 embryonic S1    97 embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS    30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    19 embryonic 1182-4H    30 embryonic GM2    18 embryonic Kc167    18 embryonic S1    97 embryonic S3    19 leg disc CME L1    19 wing disc CME-W2    20 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    20 wing disc ML-DmD32    26 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    23 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    17 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    17 tumorous blood cells mbn2    16 ovary fGS/OSS    30 ovary OSC    17 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    14 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    5 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    19 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    9 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    15 Paraquat 10 mM 48 hrs, 4-day adult    16 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD31680 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000139 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6677-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal ash2S112411 lethal | adult stage | recessive ash23 ash26 ash29 ash212 lethal | adult stage | recessive | heat sensitive ash27 lethal | larval stage | recessive ash21 ash218 lethal | prepupal stage | recessive ash22 ash25 lethal | pupal stage ash2I1 lethal | pupal stage, with Scer\GAL4tub.PU ash2GD1374 lethal | pupal stage (with ash21) ash214 lethal | pupal stage (with ash214) ash21 lethal | pupal stage | recessive ash21 ash22 ash23 ash24 ash25 ash210 ash213 ash215 ash216 ash217 lethal | pupal stage | recessive | heat sensitive ash28 ash211 lethal | recessive ash21 partially lethal - majority die ash2I2 ash2I3 partially lethal - majority die | pharate adult stage | recessive ash2S112411 partially lethal - majority die | recessive ash2S112411 viable, with Scer\GAL4pnr-MD237 ash2GD1374 Sterility Allele sterile ash2S112411 sterile | recessive ash2S112411 Other Phenotypes Allele increased cell death ash2I1 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 ash2EP3472 visible ash26 ash2S112411 visible (with ash214) ash218 visible (with ash218) ash214 Phenotype manifest in Allele adult mesothoracic segment | ectopic ash2S112411 adult prothoracic segment | ectopic ash2S112411 antenna ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash28 anterior fascicle & synapse, with Scer\GAL4elav-C155 ash2EP3472 capitellum ash26 capitellum & macrochaeta ash26 crossvein | ectopic ash2S112411 crossvein | ectopic (with In(3R)Ubx7LLatsR) ash2S112411 genitalia ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash28 haltere ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash26 ash28 haltere pedicel ash26 imaginal disc ash2I1 intersegmental nerve, with Scer\GAL4elav-C155 ash2EP3472 macrochaeta | ectopic ash26 macrochaeta | ectopic (with ash23) ash218 macrochaeta | ectopic (with ash214) ash218 macrochaeta | ectopic (with ash218) ash214 ash23 macrochaeta | ectopic (with Df(3R)ash18) ash214 ash23 ash27 metathoracic leg ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash28 metathoracic leg (with ash21) ash29 metathoracic leg (with ash29) ash21 proboscis ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash28 prothoracic leg ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash28 prothoracic segment | dorsal ash21 ash210 ash213 ash215 ash216 ash217 ash22 ash24 ash28 scabellum ash26 socket | ectopic (with ash214) ash218 socket | ectopic (with ash218) ash214 socket | ectopic (with Df(3R)ash18) ash214 thorax & macrochaeta (with ash214) ash218 thorax & macrochaeta (with ash218) ash214 thorax & macrochaeta (with Df(3R)ash18) ash214 wing ash2S112411 wing (with In(3R)Ubx7LLatsR) ash2S112411 wing & macrochaeta (with ash23) ash218 wing & macrochaeta (with ash218) ash23 wing & macrochaeta (with Df(3R)ash18) ash23 wing & sensillum campaniformium (with ash27) ash218 wing & sensillum campaniformium (with ash218) ash27 wing & sensillum campaniformium (with Df(3R)ash18) ash27 wing | somatic clone ash2I1 wing cell | somatic clone ash2I1 wing disc ash2I1 ash2S112411 wing margin | posterior ash2S112411 wing margin bristle ash2S112411 wing vein ash2S112411 wing vein | ectopic ash2S112411 wing vein | ectopic | somatic clone ash2I1 wing vein | somatic clone ash2I1 wing vein L1 | somatic clone ash2I1 wing vein L2 ash2S112411 wing vein L2 | ectopic | somatic clone ash2I1 wing vein L3 ash2S112411 wing vein L3 | ectopic | somatic clone ash2I1 wing vein L5 ash2S112411 wing vein L5 | ectopic | somatic clone ash2I1 wing vein L6 | ectopic | somatic clone ash2I1
Classical Alleles ( 26 )
For All Classical Alleles Show
Allele of ash2 Class Mutagen Stocks Known lesion ash21 amorphic allele - genetic evidence N-methyl-N'-nitro-N-nitrosoguanidine 2 Yes ash2d00279 P-element activity 1 -- ash2EY03971 P-element activity 1 -- ash2f07662 piggyBac transposase 1 -- ash22 amorphic allele - genetic evidence, hypomorphic allele - genetic evidence N-methyl-N'-nitro-N-nitrosoguanidine 0 -- ash210 ethyl methanesulfonate 0 -- ash211 ethyl methanesulfonate 0 -- ash212 ethyl methanesulfonate 0 -- ash213 ethyl methanesulfonate 0 -- ash214 ethyl methanesulfonate 0 -- ash215 ethyl methanesulfonate 0 -- ash216 ethyl methanesulfonate 0 -- ash217 ethyl methanesulfonate 0 -- ash218 PM hybrid dysgenesis 0 Yes ash23 ethyl methanesulfonate 0 -- ash24 ethyl methanesulfonate 0 -- ash25 ethyl methanesulfonate 0 -- ash26 ethyl methanesulfonate 0 -- ash27 ethyl methanesulfonate 0 -- ash28 ethyl methanesulfonate 0 -- ash29 ethyl methanesulfonate 0 -- ash2EP3472 P-element activity 0 Yes ash2I1 P-element activity 0 Yes ash2I2 0 Yes ash2I3 0 Yes ash2S112411 Delta2-3 0 --
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ash2 Class Mutagen Stocks Known lesion ash2GD1374 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ash2GL00307 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ash2KK108086 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ash2NIG.6677R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes ash2Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)ash18 (Adamson and Shearn, 1996) Df(3R)BSC492 (Christensen et al., 2008.4.15) Df(3R)crb87-5 (Adamson and Shearn, 1996) Df(3R)L16 (Gonzalez et al., 1989) Df(3R)Su9 (Gonzalez et al., 1989) Df(3R)tld-γ1 (Adamson and Shearn, 1996) In(3R)Ubx7LLatsR (Gonzalez et al., 1989)
Not disrupted in	In(3R)Ubx7L (Gonzalez et al., 1989)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1374} NA P{KK108086} NA P{NIG.6677R} NA P{TRiP.GL00307} NA P{UAS-ash2.HA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}ash2EP3472 NA P{EPgy2}ash2EY03971 NA P{Mae-UAS.6.11}B0404B0404 NA P{XP}ash2d00279 NA miscellaneous insertions PBac{WH}ash2f07662 NA insertion of enhancer trap P{lacW}ash2S112411 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
chromatin-mediated maintenance of transcription inferred from mutant phenotype (LaJeunesse and Shearn, 1995) histone H3-K4 methylation inferred from direct assay (Mohan et al., 2011) inferred from mutant phenotype (Beltran et al., 2007, Hallson et al., 2012) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Amoros et al., 2002) imaginal disc-derived wing vein specification inferred from genetic interaction with bs (Angulo et al., 2004) inferred from genetic interaction with kni (Angulo et al., 2004) inferred from genetic interaction with net (Angulo et al., 2004) inferred from mutant phenotype (Angulo et al., 2004)
Cellular Component
CV term Evidence References
MLL1/2 complex inferred from direct assay (Mohan et al., 2011) MLL3/4 complex inferred from direct assay (Mohan et al., 2011) nucleus inferred from direct assay AND inferred from sequence or structural similarity (Adamson and Shearn, 1996) polytene chromosome inferred from direct assay (Beltran et al., 2007) Set1C/COMPASS complex inferred from direct assay (Ardehali et al., 2011, Hallson et al., 2012, Mohan et al., 2011)
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
zinc ion binding inferred from electronic annotation with InterPro:IPR001965 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
ash2 - Actn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - CG10641 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - CG1532 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - CG17387 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - CG6434 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) ash2 - CG7049 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Cfp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Dpy-30L1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Mohan et al., 2011, Guruharsha et al., 2011) ash2 - Hcf anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Lpt anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Mnn1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Ncoa6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Pa1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Plod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Rbbp5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23, Mohan et al., 2011) ash2 - RpS21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Rpb8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Set1 cosedimentation, anti tag western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, pull down (Hallson et al., 2012, Mohan et al., 2011) ash2 - Tango4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Treh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) ash2 - Utx anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - Wdr82 anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - ptip anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - trr anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - trx anti tag coimmunoprecipitation, peptide massfingerprinting (Mohan et al., 2011) ash2 - wds anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23, Mohan et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ash2 allele Gene References ash21 Antp (Adamson and Shearn, 1996) CycE (Brumby et al., 2002) sktl (Cheng and Shearn, 2004) ash22 fs(1)h (Shearn, 1989) sktl (Cheng and Shearn, 2004) ash211 Antp (Adamson and Shearn, 1996) ash212 Antp (Adamson and Shearn, 1996) ash218 Antp (Adamson and Shearn, 1996) CycE (Brumby et al., 2002) sktl (Cheng and Shearn, 2004) Ubx (LaJeunesse and Shearn, 1995) ash2S112411 cv-2 (Amoros et al., 2002) unspecified Antp (Adamson and Shearn, 1996) dm (Secombe et al., 2007) E(z) (Bajusz et al., 2001) ph-p (Fauvarque et al., 1995)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67862 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000139 DroID - A comprehensive database of gene and protein interactions. • FBgn0000139 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42936
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6KWKCF Dipteran inclusive ortholog search EOG6M920Q Insect inclusive ortholog search EOG6547TS Arthropod inclusive ortholog search EOG66DJHV Metazoa inclusive ortholog search EOG65BD7M
Orthologs in Drosophila Species (EOG6KWKCF)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\ash2     Drosophila simulans   Dsim\GD21115 Y   Drosophila sechellia   Dsec\GM26615 Y   Drosophila erecta   Dere\GG11306 Y   Drosophila yakuba   Dyak\GE23503 Y   Drosophila ananassae   Dana\GF17218 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26848 Y   Drosophila persimilis   Dper\GL13614 Y   Drosophila willistoni   Dwil\GK10965 Y   Drosophila virilis   Dvir\GJ23645 Y   Drosophila mojavensis   Dmoj\GI24014 Y   Drosophila grimshawi   Dgri\GH18438 Y
Orthologs in non-Drosophila Dipterans (EOG6M920Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL003771   Anopheles gambiae Malaria mosquito  Agam\AGAP003705   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ005772
Orthologs in non-Dipteran Insects (EOG6547TS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\ash2   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007791   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH19439   Atta cephalotes Leafcutter ant  Acep\ACEP24630   Camponotus floridanus Florida carpenter ant  Cflo\CFLO15339   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17173   Linepithema humile Argentine ant  Lhum\LH18736   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17162   Solenopsis invicta Red fire ant  Sinv\SINV25271   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000122   Acyrthosiphon pisum Pea aphid  Apim\ACYPI080213   Acyrthosiphon pisum Pea aphid  Apim\ACYPI085715   Bombyx mori Silkmoth  Bmor\BGIBMGA008025   Pediculus humanus Human body louse  Phum\PHUM569810   Tribolium castaneum Red flour beetle  Tcas\TC011210
Orthologs in non-Insect Arthropods (EOG66DJHV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COI   Ixodes scapularis Deer tick  Isca\ISCW015044
Orthologs in non-Arthropod Metazoa (EOG65BD7M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ash-2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006059gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004112gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018423gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_013013gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024999gn   Danio rerio Zebrafish  Drer\ash2l   Xenopus tropicalis Western clawed frog  Xtro\ash2l   Gallus gallus Domestic chicken  Ggal\ASH2L   Mus musculus House mouse  Mmus\Ash2l   Rattus norvegicus Norway rat  Rnor\Ash2l   Homo sapiens Human  Hsap\ASH2L
Human Orthologs (1)
Gene OMIM HGNC Hsap\ASH2L
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21115 Drosophila sechellia Dsec\GM26615 Drosophila erecta Dere\GG11306 Drosophila yakuba Dyak\GE23503 Drosophila ananassae Dana\GF17218 Drosophila pseudoobscura pseudoobscura Dpse\GA26848 Drosophila persimilis Dper\GL13614 Drosophila willistoni Dwil\GK10965 Drosophila virilis Dvir\GJ23645 Drosophila mojavensis Dmoj\GI24014 Drosophila grimshawi Dgri\GH18438
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	4584 red1 e1 ash21/TM6B, Tb1 35388 y1 sc* v1; P{TRiP.GL00307}attP2/TM3, Sb1 15697 y1 w67c23; P{EPgy2}ash2EY03971/TM3, Sb1 Ser1
Harvard	- P{XP}ash2d00279 - PBac{WH}ash2f07662
Kyoto	107807 red1 e1 ash21/TM6B, Tb1
VDRC	v100718 P{KK108086}VIE-260B v7141 w1118; P{GD1374}v7141
Genomic Clones ( 2 )
	BACR35N03 BACR47D16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 198 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD31680 SD28012
Other clones	DPiM_LD31680 DPiM_SD28012 MIP16976
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT02120 AT10273 LD28975 LD29982 LD35923 RE02945 RE03678 RE09474 RE10214 RE32089 RE50329 RE60940 RE65484
Other clones	12730 53526 69991 154691 bs20e11 Dmel_dig_001202_0708_1417 Dmel_dig_014995_0705_0890 Dmel_dig_019914_0580_2749 Dmel_neb_011114_0419_2334 EC31475 EK054661 EK114125 EK197462 EK258129 LD46053 RP003000645 RP003010997 RP003022315 RP003034878 RP003036415 RP003041426 RP003051580 RP003053293 RP003058544 RP003067328 RP003075269 RP003076743 RP003084624 RP003086736 RP003091065 RP003107029 RP003107671 RP003116395 RP003119856 RP003122091 RP003122564 RP003122585 RP003132506 RP003140879 RP003145049 RP003151563 RP003154338 RP003155222 RP003160648 RP003162308 RP003168127 RP003173063 RP003174990 RP003182903 RP003188498 RP003193679 RP003200206 RP003201619 RP003206830 RP003217471 RP003225034 RP003232576 RP003233787 RP003238043 RP003239149 RP003241424 RP003243055 RP003243116 RP003246996 RP003249302 RP003249762 RP003254400 RP003257222 RP003257527 RP003263918 RP003264854 RP003267095 RP003269837 RP003270640 RP003272882 RP003273794 RP003275506 RP003276786 RP003277580 RP003279362 RP003282096 RP003284189 RP003284744 RP003284919 RP003294317 RP003295768 RP003297131 RP003300359 RP003304120 RP003307371 RP003317890 RP003325346 RP003327952 RP003333477 RP003340373 RP003341308 RP003342104 RP003354440 RP003364405 RP003373432 RP003378277 RP003381456 RP003386320 RP003387102 RP003387961 RP003397876 RP003398492 RP003400133 RP003401440 RP003409386 RP003416869 RP003418271 RP003418687 RP003438573 RP003438929 RP003441889 RP003444342 RP003445434 RP003447324 RP003456567 RP003459248 RP003463934 RP003470078 RP003472049 RP003473634 RP003477481 RP003478724 RP003483891 RP003484605 RP003487832 RP003490860 RP003491250 RP003493431 RP003500960 RP003504530 RP003508030 RP003509740 RP003510693 RP003512594 RP003517790 RP003522107 RP003525467 RP003528641 RP003533458 RP003538277 RP003548386 RP003552150 RP003553688 RP003555131 RP003558270 RP003559120 RP003561242 RP003564260 RP003567313 RP003576029 RP003578638 RP003578703 RP003579885 RP003581841 RP003584501 RP003585158 RP003594814 RP003596748 RP003607494 RP003607882 RP003610507 RP003615563 RP003632882 RP003635911 RP003650214 RP003653053 RP003654234 RP003660421 RP003660756 RP003663830 RP003666410 RP003677585 RP003677998 RP003680237 RP003682728
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000139 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42936
Antibody Information
	polyclonal (Adamson and Shearn, 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ash2 CG6677 (Kodira, 1999.11)
Source for database merge of	Source for merge of: l(3)S112411 ash2 (Amoros et al., 2002)
Additional comments	"ash2" mutations complement alleles of "aor". (Gonzalez et al., 1989)
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for ash2 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069). (Filion et al., 2010) ash2 has a role in wing development. (Amoros et al., 2002) Phenotypes of ash2 mutants that survive to the end of metamorphosis reveal that the ash2 gene product does not solely regulate homeotic selector genes. Pattern formation abnormalities are observed on mutant legs and wings. ash2 is a nuclear protein that is present in larval imaginal discs at a low level and at a much higher level in imaginal discs after puparium formation. The size of the ash2 protein changes dramatically at the end of the third larval instar suggesting that the protein has different functions in larvae than in pupae. (Adamson and Shearn, 1996) ash1 and ash2 are trans-regulatory elements of homeotic selector gene regulation. ash2 is required for the functioning of the Ubx ABX/BX enhancer element, regulation involves different cis-regulatory regions that than of ash1. (LaJeunesse and Shearn, 1995) Mutations in ash2 give rise to homeotic transformations. (Gonzalez et al., 1989) Genetic tests were used to confirm that ash1 and ash2 belong to a functionally related class of genes, mutations in which cause a wide variety of homeotic transformations that are similar to the transformations caused by trx. Double heterozygotes of ash2 alleles and Df(3R)red-P93 show a significant penetrance of homeotic transformations. (Shearn, 1989) A member of the trithorax group of genes.   Most alleles homozygous lethal; stage of lethality variable. Surviving and pharate adults display transformations similar to those indicated for ash1. Homozygous lethal; labial and wing discs appear normal; eye-antenna, prothoracic, haltere, leg and genital discs homeotically transformed. Disc and cell autonomous.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_170160 NM_176558 NM_001260363 RefSeq (Proteins) • NP_733024 NP_788735 NP_001247292 DDBJ / EMBL / GenBank • AA951292 AAC47328 AAF56320 AAK93227 AAN14010 AAO41602 AFH06609 AI946074 AW943098 AY051803 AZ300978 BI633838 CZ471498 CZ490398 U73809 Entrez Gene - A searchable database of RefSeq genes. • 42936 UniProtKB/TrEMBL • Q8IMW1 Q9VC55 Q94545
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • SPla/RYanodine receptor subgroup (IPR018355) B30.2/SPRY domain (IPR001870) Zinc finger, PHD-type (IPR001965) SPla/RYanodine receptor SPRY (IPR003877) Zinc finger, FYVE/PHD-type (IPR011011) Zinc finger, PHD-type, conserved site (IPR019786) Concanavalin A-like lectin/glucanases superfamily (IPR008985) Zinc finger, RING/FYVE/PHD-type (IPR013083) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD31680
Linkouts
	BioGRID - A database of protein and genetic interactions • 67862 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0000139 DroID - A comprehensive database of gene and protein interactions. • FBgn0000139 DRSC - Results from RNAi screens. • FBgn0000139 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000139 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6677-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000139 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42936 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /polycomb/ash2-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42936 modMine - Data generated by the modENCODE project. • FBgn0000139
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	291.8 (Adamson and Shearn, 1996) 703   1124/11 (Deak et al., 1997) ash-2 (Bloyer et al., 1999, Fauvarque et al., 1995, Gindhart and Kaufman, 1995, Gonzalez et al., 1989) ASH2 (Papoulas et al., 1998, Schwartz and Pirrotta, 2007, Filion et al., 2010, Lloret-Llinares et al., 2012) ash2 (Blanco et al., 2010, Ji et al., 2012, Carbonell et al., 2007, Christensen et al., 2008.4.15, Grieder et al., 2007, Smulders-Srinivasan et al., 2010, Vázquez et al., 2008, de la Paz Fernandez et al., 2007, Wang et al., 2007, Angulo et al., 2004, Khan et al., 2009, Beltran et al., 2007, Blanco et al., 2008, Ghezzi et al., 2010, Di Stefano et al., 2011, Loop et al., 2004, Japanese National Institute of Genetics, 2012.5.21) Ash2 (Secombe et al., 2007, Hallson et al., 2012, Kapelnikov et al., 2008, Guenin et al., 2010, Ardehali et al., 2011, Mohan et al., 2011) CG6677 (Klebes et al., 2005, Kraut et al., 2001, Kraut et al., 2001, Guertin et al., 2006, Bienz, 2006, Grieder et al., 2007, Keleman et al., 2009.8.5, Ni et al., 2011.7.19, Ardehali et al., 2011, Mohan et al., 2011) l(3)112411 (Amoros et al., 2002) l(3)S112411   l(3)SG65   mad
Name Synonym	absent, small, or homeotic discs 2   absent, small or homeotic discs 2 (Beltran et al., 2007) many abnormal discs
Secondary FlyBase IDs
	FBgn0060470
References ( 96 )
Generate a list of	All references relating to this gene ( 96 )
List References by type	Research paper  ( 55 ) Supplementary material  ( 7 ) Review  ( 8 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 7 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Book  ( 1 )
Recent research papers ( 9 )
	Chauhan et al., 2012, Development 139(11): 1997--2008 Histone Recognition and Nuclear Receptor Co-Activator Functions of Drosophila Cara Mitad, a Homolog of the N-Terminal Portion of Mammalian MLL2 and MLL3. [FBrf0218263] Hallson et al., 2012, Genetics 190(1): 91--100 dSet1 Is the Main H3K4 Di- and Tri-Methyltransferase Throughout Drosophila Development. [FBrf0217167] Herz et al., 2012, Genes Dev. 26(23): 2604--2620 Enhancer-associated H3K4 monomethylation by Trithorax-related, the Drosophila homolog of mammalian Mll3/Mll4. [FBrf0220205] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Lloret-Llinares et al., 2012, Nucleic Acids Res. 40(19): 9493--9505 dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes. [FBrf0219748] Ardehali et al., 2011, EMBO J. 30(14): 2817--2828 Drosophila Set1 is the major histone H3 lysine 4 trimethyltransferase with role in transcription. [FBrf0214333] Di Stefano et al., 2011, Genes Dev. 25(1): 17--28 Functional antagonism between histone H3K4 demethylases in vivo. [FBrf0212709] Mohan et al., 2011, Mol. Cell. Biol. 31(21): 4310--4318 The COMPASS Family of H3K4 Methylases in Drosophila. [FBrf0216343] Pérez-Lluch et al., 2011, Nucleic Acids Res. 39(11): 4628--4639 Genome-wide chromatin occupancy analysis reveals a role for ASH2 in transcriptional pausing. [FBrf0213882] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...