Gene model reviewed during 5.45
Gene model reviewed during 5.49
Gene model reviewed during 6.24
There is only one protein coding transcript and one polypeptide associated with this gene
442 (aa); 49 (kD)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bam using the Feature Mapper tool.
bam transcript is expresed in two waves: the first in spermatogonia, and the second in early spermatocytes. bam transcript, but not protein, is detected in primary spermatocytes, suggesting that bam protein translation is downregulated.
bam transcripts are expressed throughout oogenesis and in 0-2hr embryos but are absent by 4hr of development. They are also detected in dissected ovaries and testes. In ovaries, bam transcripts are expressed in cystoblasts and/or very early cystocytes. Later they are detected in stage 10 nurse cells, are transferred to the oocyte at the time of nurse cell breakdown, and persist in the mature egg and the early embryo..
GBrowse - Visual display of RNA-Seq signalsView Dmel\bam in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Forced premature expression of bam in early male germ cells (stem cells, gonialblasts and spermatogonia) leads to accumulation of male germ cells at the single-cell stage and then death of the early male germ cells.
The down-regulation of bam protein is essential for the initiation of cystocyte differentiation into functional egg chambers.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Mosaic analysis demonstrates bam and bgcn act autonomously in the germline to restrict germ cell proliferation during spermatogenesis. Results suggest bam and bgcn regulate progression through the male germline stem cell lineage by cell-intrinsically restricting the proliferation of amplifying germ cells. bgcn function is not required for the proper expression of bam protein.
Mutations in bam disrupt cyst formation producing tumorous egg chambers.
bam is required to promote incomplete cytokinesis and activate fusome growth.
Mutants display germline hyperplastic phenotype.
reference: Spradling and McKearin, personal communication.