FlyBase Gene Report: Dmel\bib

General Information

Symbol

Dmel\bib

Species

D. melanogaster

Name

big brain

Annotation Symbol

CG4722

Feature Type

protein_coding_gene

FlyBase ID

FBgn0000180

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

big brain (bib) encodes a non-selective cation channel in the aquaporin family. Its function is required for optimal Notch signaling to restrain neurogenesis. [Date last reviewed: 2018-09-06] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

30F5-30F5

Sequence location

2L:9,984,645..9,995,538 [+]

Recombination map

2-38

RefSeq locus

NT_033779 REGION:9984645..9995538

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables cation channel activity

inferred from direct assay

(Yanochko and Yool, 2002)

NOT enables ion channel activity

inferred from direct assay

(Tatsumi et al., 2009)

NOT enables water transmembrane transporter activity

inferred from direct assay

(Tatsumi et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables channel activity

inferred from sequence or structural similarity with FLYBASE:Drip; FB:FBgn0015872

(Kaufmann et al., 2005)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in cell-cell adhesion

inferred from direct assay

(Tatsumi et al., 2009)

involved_in mesoderm development

inferred from mutant phenotype

(Corbin et al., 1991)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in transmembrane transport

inferred from sequence or structural similarity with FLYBASE:Drip; FB:FBgn0015872

(Kaufmann et al., 2005)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in integral component of membrane

inferred from direct assay

(Tatsumi et al., 2009, Doherty et al., 1997)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in integral component of membrane

inferred from biological aspect of ancestor with PANTHER:PTN002709492

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002709492

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

AQUAPORINS

Protein Family (UniProt)

Belongs to the MIP/aquaporin (TC 1.A.8) family. (P23645)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

big brain (bib) encodes a non-selective cation channel in the aquaporin family. Its function is required for optimal Notch signaling to restrain neurogenesis. [Date last reviewed: 2018-09-06]

Gene Group (FlyBase)

AQUAPORINS -: Aquaporins (AQPs) are integral membrane proteins with a central pore formed form two highly conserved hydrophobic regions, NPA boxes, which contain the signature Asp-Pro-Ala motif. They selectively allow the transfer of water across the membranes. (Adapted from PMID:22542572).

Protein Function (UniProtKB)

Essential for proper differentiation of ectoderm. Acts synergistically with neurogenic locus proteins Notch and Delta during the separation of neural and epidermal cell lineages in response to the lateral inhibition signal. Voltage-insensitive monovalent cation channel. Ion transport is blocked by the presence of divalent cations.

(UniProt, P23645)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

bib: big brain

Recessive embryonic lethal, a neurogenic mutant. Homozygotes fail to form ventral, lateral, and most of the cephalic epidermis. Central nervous system hypertrophied by recruitment of presumptive epidermal cells, but exhibiting considerable architectural normality. Most epidermal sense organs can be recognized in electron microscope preparations; chordotonal organs prevalent but with abnormal structures, perhaps owing to disrupted attachment. Supernumerary peripheral elements formed by recruitment of presumptive epidermal cells [Hartenstein and Campos-Ortega, 1986, Wilhelm Roux's Arch. Dev. Biol. 195: 210-21 (fig.). Cuticular clones of cells homozygous for bib1 or bib6 exhibit normal development (Dietrich and Campos-Ortega, 1984, J. Neurogenet. 1: 315-32). Effects on central nervous system intermediate with respect to mutations at other neurogenic loci. Insensitive to changes in dosage of other neurogenic genes (de la Concha, Dietrich, Weigel, and Campos-Ortega, 1988, Genetics 118: 499-508).

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\bib for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

bib-RA

FBtr0079929

NM_057489

3276

696

bib-RB

FBtr0329984

NM_001273384

3276

737

Additional Transcript Data and Comments

Reported size (kB)

3.1 (northern blot)

(Rao et al., 1990)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

bib-PA

FBpp0079519

76.5

696

8.79

NP_476837

AAF52844

bib-PB

FBpp0303019

80.8

737

9.09

NP_001260313

AGB92848

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

700 (aa)

(Rao et al., 1990)

Comments

External Data

Post Translational Modification

Phosphorylated at its C-terminus.

(UniProt, P23645)

Domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

(UniProt, P23645)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bib using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

bib_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

epithelial cell

• cytoplasm | apical

Comment: clustered apical distribution

(Lecuyer et al., 2007)

epithelium

• cytoplasm | apical

(Lecuyer et al., 2007)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 5

organism | ubiquitous

Comment: early stage 5

(Rao et al., 1990)

organism

Comment: late stage 5

(Rao et al., 1990)

embryonic stage 5 -- 12

ectoderm | restricted

(Rao et al., 1990)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 12

mesoderm

(Rao et al., 1990)

midline primordium | subset

(Kearney et al., 2004)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

pars intercerebralis primordium

(Fisher et al., 2012)

pericardial cell primordium

Comment: reported as pericardial cell specific anlage

(Fisher et al., 2012)

stomatogastric nervous system primordium

(Fisher et al., 2012)

tracheal primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic pars intercerebralis

(Fisher et al., 2012)

embryonic stomatogastric nervous system

(Fisher et al., 2012)

embryonic optic lobe primordium

(Fisher et al., 2012)

Additional Descriptive Data

bib transcript is detected in all somatic cells at the start of cellularization. By the completion of cellularization, bib is absent from a 17-cell-wide ventral strip which includes the presumptive mesoderm cells and two rows of ectodermal cells. Through stage 8, bib expression is maintained in most ectodermal tissue. During neuroblast delamination at late stage 8-stage 9, neuroblasts which segregate from the ectodermal layer lose bib expression. At the end of germ-band extension, the mesoderm begins to express bib. By late stage 11, bib expression disappears first from the epidermis, then the mesoderm. After stage 12, bib is barely detectable in the embryo.

(Rao et al., 1990)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 7

stomodeal invagination

(Doherty et al., 1997)

amnioproctodeal invagination

(Doherty et al., 1997)

embryonic stage 7 -- 17

ectoderm

(Doherty et al., 1997)

embryonic stage 11

ventral midline of embryo

(Doherty et al., 1997)

tracheal pit

(Doherty et al., 1997)

embryonic stage 13

Malpighian tubule

(Doherty et al., 1997)

amnioserosa

(Doherty et al., 1997)

embryonic precursor cell of adult muscle | precursor

(Doherty et al., 1997)

embryonic/larval dorsal vessel

(Doherty et al., 1997)

embryonic stage 13 -- 15

embryonic/larval posterior midgut

(Doherty et al., 1997)

embryonic stage 16

ventral nerve cord | subset

(Doherty et al., 1997)

larval stage

embryonic/larval brain

(Park et al., 2008)

larval ventral nerve cord

(Park et al., 2008)

wing disc

(Park et al., 2008)

ventral thoracic disc

(Park et al., 2008)

eye disc

(Park et al., 2008)

third instar larval stage

flight muscle | precursor

(Doherty et al., 1997)

adepithelial cell of wing disc | restricted

(Doherty et al., 1997)

sense organ of wing disc

(Doherty et al., 1997)

proneural cluster of wing disc

(Doherty et al., 1997)

wing vein | precursor

(Doherty et al., 1997)

pupal stage P6

eo neuron

Comment: reference states 24 hr APF

(Doherty et al., 1997)

epidermis

Comment: reference states 24 hr APF

(Doherty et al., 1997)

pupal epidermis

Comment: reference states 24 hr APF

(Doherty et al., 1997)

thecogen cell

Comment: reference states 24 hr APF

(Doherty et al., 1997)

tormogen cell

Comment: reference states 24 hr APF

(Doherty et al., 1997)

trichogen cell

Comment: reference states 24 hr APF

(Doherty et al., 1997)

adult stage

adult hindgut

(Park et al., 2008)

adult brain cell body rind

(Park et al., 2008)

Additional Descriptive Data

bib protein is expressed in tissues that give rise to neural precursors, and in tissues that require neurogenic gene function.

(Doherty et al., 1997)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in integral component of membrane

inferred from direct assay

(Tatsumi et al., 2009, Doherty et al., 1997)

Expression Deduced from Reporters

Reporter: P{bib-lacZ.P}

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval brain

(Park et al., 2008)

larval ventral nerve cord

(Park et al., 2008)

wing disc | restricted

(Park et al., 2008)

ventral thoracic disc

(Park et al., 2008)

eye disc | restricted

(Park et al., 2008)

adult stage

adult hindgut

(Park et al., 2008)

Reporter: P{GawB}lncRNA:CR13130^NP5149

Stage

Tissue/Position (including subcellular localization)

Reference

pupal stage

leg | restricted

(Tajiri et al., 2010)

Reporter: P{lacZ}6E1

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage

tarsal segment | distal | striped

(Capilla et al., 2012)

prepupal stage P4

metatarsus | distal

(Pueyo and Couso, 2011)

tarsal segment 2 | distal

(Pueyo and Couso, 2011)

tarsal segment 3 | distal

(Pueyo and Couso, 2011)

tarsal segment 4 | distal

(Pueyo and Couso, 2011)

Reporter: P{lacZ}bib⁴¹⁶³

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\bib in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\bib

View exonic expression by tissue for Dmel\bib

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000180

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0000180

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4722-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4722

Flygut - An atlas of the Drosophila adult midgut

FBgn0000180

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\bib

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 23 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of bib

Transgenic constructs containing regulatory region of bib

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene disrupted in

Df(2L)BSC50

(Deal and Cook, 2004.5.27)

Df(2L)BSC205

(Christensen and Cook, 2006.8.30)

Df(2L)BSC251

(Christensen and Cook, 2007.3.22)

Df(2L)BSC689

(Christensen et al., 2008.12.28)

Gene not disrupted in

Tp(2;3)dp^h27

(de la Concha et al., 1988)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

bib^GD16290

lethal | neurogenic phenotype | recessive

bib^neo91

lethal | pupal stage, with Scer\GAL4^pnr-MD237

bib^GD16290

lethal | recessive

lethal - all die during embryonic stage | neurogenic phenotype

lethal - all die during embryonic stage | neurogenic phenotype | recessive

lethal - all die during embryonic stage | recessive

bib^unspecified

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

bib^KK112898

viable, with Scer\GAL4^pnr-MD237

bib^GD1049

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

bib^GD1049

abnormal size, with Scer\GAL4^en-e16E

bib^JF02771

increased cell number | embryonic stage

some die during embryonic stage | neurogenic phenotype

some die during embryonic stage | recessive

visible, with Scer\GAL4^pnr-MD237

bib^GD1049

visible, with Scer\GAL4^ptc-559.1

bib^dsRNA.UAS

visible, with Scer\GAL4^sca-109-68

bib^UAS.cDa

visible, with Scer\GAL4^sca-537.4

wild-type

bib^+t15

Phenotype manifest in

Allele

adult optic lobe | embryonic stage

bib¹

cardioblast | embryonic stage

bib¹

chaeta | increased number, with Scer\GAL4^pnr-MD237

bib^GD1049

ectoderm

bib^III9-5

embryonic/first instar larval cuticle | ventral (with bib⁷)

bib⁸

embryonic/first instar larval cuticle | ventral (with bib⁸)

bib⁷

embryonic/larval dorsal vessel | precursor

bib¹

embryonic/larval nervous system | somatic clone

bib¹

embryonic/larval oenocyte precursor

bib¹

embryonic/larval salivary gland | embryonic stage

bib¹

embryonic/larval somatic muscle

embryonic/larval somatic muscle | germline clone

bib⁷

embryonic cardioblast | increased number

bib^S1538

embryonic epidermis

embryonic epidermis | ventral

bib^unspecified

embryonic Malpighian tubule

bib¹

embryonic myoblast

bib¹

embryonic ostial cardioblast | increased number

bib^S1538

external sensory organ precursor cell | embryonic stage

bib^III9-5

eye photoreceptor cell | somatic clone | increased number

bib¹

heart primordium

bib^S1538

macrochaeta, with Scer\GAL4^sca-109-68

bib^UAS.cDa

macrochaeta, with Scer\GAL4^sca-537.4

macrochaeta | ectopic, with Scer\GAL4^sca-537.4

bib^EP2278

macrochaeta | somatic clone

mesothoracic tergum, with Scer\GAL4^pnr-MD237

bib^GD1049

microchaeta, with Scer\GAL4^sca-109-68

bib^UAS.cDa

microchaeta, with Scer\GAL4^sca-537.4

bib^UAS.cDa

microchaeta | somatic clone

multidendritic neuron | ectopic

bib¹

multidendritic neuron | ectopic | somatic clone

bib¹

multidendritic neuron | somatic clone

bib¹

nervous system

bib^unspecified

neuroblast | embryonic stage

bib¹

peripheral glial cell

bib¹

presumptive embryonic/larval central nervous system

presumptive embryonic/larval nervous system

bib¹

presumptive embryonic/larval nervous system (with bib⁷)

bib⁸

presumptive embryonic/larval nervous system (with bib⁸)

bib⁷

rhabdomere | somatic clone | increased number

bib¹

scutellar bristle | ectopic, with Scer\GAL4^ptc-559.1

bib^dsRNA.UAS

sense organ, with Scer\GAL4^sca-109-68

bib^UAS.cDa

sense organ, with Scer\GAL4^sca-537.4

bib^UAS.cDa

sensory mother cell

bib¹

stomatogastric nervous system | embryonic stage

bib¹

wing | posterior, with Scer\GAL4^en-e16E

bib^JF02771

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\AQP1

5 of 15

Yes

4 of 15

4 of 15

4 of 15

3 of 15

3 of 15

3 of 15

3 of 15

3 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Aqp1

6 of 15

Yes

5 of 15

5 of 15

4 of 15

4 of 15

4 of 15

3 of 15

2 of 15

2 of 15

2 of 15

1 of 15

Rattus norvegicus (Norway rat) (10)

Rnor\Aqp1

4 of 13

Yes

Rnor\Aqp4

4 of 13

Yes

Rnor\Mip

4 of 13

Yes

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (17)

Xtro\aqp2

4 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Xtro\aqp9

2 of 12

Xtro\LOC100487834

2 of 12

Yes

Xtro\LOC100491830

2 of 12

Xtro\LOC101733960

2 of 12

Yes

Xtro\aqp7

1 of 12

Xtro\aqp10

1 of 12

Xtro\LOC100497434

1 of 12

Yes

Xtro\mindy4

1 of 12

Danio rerio (Zebrafish) (19)

Drer\aqp1a.1

5 of 15

Yes

Drer\aqp4

5 of 15

Yes

4 of 15

4 of 15

4 of 15

3 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

Yes

Drer\aqp7

1 of 15

Drer\aqp9a

1 of 15

Yes

Drer\aqp10a

1 of 15

Drer\aqp10b

1 of 15

Drer\mindy4b

1 of 15

Drer\si:dkey-30h22.11

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (9)

Cele\aqp-6

5 of 15

Yes

4 of 15

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (33)

Atha\PIP1;4

2 of 9

Yes

Atha\PIP1;5

2 of 9

Yes

Atha\PIP1A

2 of 9

Yes

Atha\PIP1B

2 of 9

Yes

Atha\PIP1C

2 of 9

Yes

Atha\PIP2;4

2 of 9

Yes

Atha\PIP2;5

2 of 9

Yes

Atha\PIP2;8

2 of 9

Yes

Atha\PIP2A

2 of 9

Yes

Atha\PIP2B

2 of 9

Yes

Atha\PIP2E

2 of 9

Yes

Atha\PIP3

2 of 9

Yes

Atha\RD28

2 of 9

Yes

Atha\AT1G52180

1 of 9

Yes

Atha\AT2G16835

1 of 9

Yes

Atha\AT2G29870

1 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\AQY1

6 of 15

Yes

5 of 15

2 of 15

2 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPAC977.17

2 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919074R )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18385

Drosophila persimilis

Dper\GL18952

Drosophila willistoni

Dwil\GK15582

Drosophila virilis

Dvir\bib

Drosophila mojavensis

Dmoj\GI17582

Drosophila grimshawi

Dgri\GH11064

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091503KE )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17019221

Glossina morsitans

Tsetse fly

Gmor\GMOY012056

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_06872

Mayetiola destructor

Hessian fly

Mdes\Mdes010740

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL004741

Anopheles darlingi

American malaria mosquito

Adar\ADAC010640

Anopheles gambiae

Malaria mosquito

Agam\AGAP008767

Anopheles gambiae

Malaria mosquito

Agam\AGAP008766

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016447

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0N19 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA005817

Bombyx mori

Silkmoth

Bmor\BGIBMGA008236

Danaus plexippus

Monarch butterfly

Dple\DPOGS211603-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL009654

Apis florea

Little honeybee

Aflo\2625726532

Apis mellifera

Western honey bee

Amel\bib

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18488

Bombus terrestris

Buff-tailed bumblebee

Bter\15212590

Linepithema humile

Argentine ant

Lhum\LH19864

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000118

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001428

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_09109

Tribolium castaneum

Red flour beetle

Tcas\TC010832

Pediculus humanus

Human body louse

Phum\PHUM617300

Rhodnius prolixus

Kissing bug

Rpro\RPRC013021

Rhodnius prolixus

Kissing bug

Rpro\RPRC011100

Cimex lectularius

Bed bug

Clec\CLEC002337

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI010017

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01212

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BFY )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR015755

Ixodes scapularis

Black-legged tick

Isca\ISCW019613

Tetranychus urticae

Two-spotted spider mite

Turt\tetur17g00420

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0I3Y )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ciona intestinalis

Vase tunicate

Cint\LOC100181312

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (7)

Gene Symbol

Score

Source

Alignment

6 of 10

6 of 10

5 of 10

4 of 10

4 of 10

4 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

AQP1; aquaporin 1 (Colton blood group)

5 of 15

107776

BLOOD GROUP, COLTON SYSTEM; CO

AQP2; aquaporin 2

4 of 15

107777

DIABETES INSIPIDUS, NEPHROGENIC, AUTOSOMAL

nephrogenic diabetes insipidus

AQP4; aquaporin 4

4 of 15

600308

MIP; major intrinsic protein of lens fiber

4 of 15

154050

CATARACT 15, MULTIPLE TYPES; CTRCT15

cataract 15 multiple types

AQP3; aquaporin 3 (Gill blood group)

3 of 15

600170

GIL BLOOD GROUP

AQP5; aquaporin 5

3 of 15

600442

PALMOPLANTAR KERATODERMA, BOTHNIAN TYPE; PPKB

Bothnian type palmoplantar keratoderma

3 of 15

3 of 15

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bib

enhanceable

(Doherty et al., 1997)

bib

suppressible

(Lieber et al., 1993)

bib

enhanceable

(Doherty et al., 1997)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

bib

(Doherty et al., 1997)

Dref

suppressible

bib

(Park et al., 2008)

fru

enhanceable

bib

(Goto et al., 2011)

enhanceable

bib

(Doherty et al., 1997)

Nsf2

enhanceable

bib

(Stewart et al., 2001)

pnr

suppressible

bib

(Pena-Rangel et al., 2002)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60424

DroID - A comprehensive database of gene and protein interactions.

FBgn0000180

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34330

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-38

Cytogenetic map

30F5-30F5

Sequence location

2L:9,984,645..9,995,538 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30F5-30F5

Limits computationally determined from genome sequence between P{EP}CG5899^EP701 and P{EP}CG4747^EP594&P{lacW}l(2)k13305^k13305

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

30F-30F

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000, Rao et al., 1990)

30A9-30F6

(Tearle and Nusslein-Volhard, 1987)

Experimentally Determined Recombination Data

Location

2-38

(FlyBase, 2016)

2-32.4

(Comeron et al., 2012)

2-34.7

(Tearle and Nusslein-Volhard, 1987)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Bloomington

1595

bib¹/CyO

14478

w^*; P{SUPor-P}bib^KG06241

27691

y¹ v¹; P{TRiP.JF02771}attP2

23729

y¹ w^67c23; Mi{ET1}bib^MB00798

34502

bib² cn¹ bw¹ speck¹/CyO

57493

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04807}attP2/TM3, Sb¹

Kyoto

106405

bib¹/CyO

113424

w^*; P{GawB}bib^NP4281 / CyO, P{UAS-lacZ.UW14}UW14

VDRC

v103327

P{KK112898}VIE-260B

v8893

w¹¹¹⁸; P{GD1049}v8893

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (53)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD45157

Other clones

DPiM_LD45157

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0000180

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0000180

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34330

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Tatsumi et al., 2009)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

Other Comments

Dvir\bib is cloned, sequenced and compared to bib : the channel domain and both cytoplasmic domains are highly conserved.

(Burris et al., 1998)

bib function is required autonomously in epidermal precursors to prevent neural development.

(Doherty et al., 1997)

Alleles of bib and neur show no interaction with da in ovary development.

(Cummings and Cronmiller, 1994)

bib is a neurogenic gene required initially to ensure the correct number of PNS precursors. bib is not required for the late epidermal maintenance function.

(Bodmer et al., 1993)

The embryonic phenotype of neurogenic mutations was examined in most tissues using Ecol\lacZ enhancer trap lines. All alleles examined show defects in many organs from all three germ layers. At least for ectodermally and endodermally derived tissues, neurogenic gene function is primarily involved in interactions among cells that need to acquire or maintain an epithelial phenotype.

(Hartenstein et al., 1992)

Recessive embryonic lethal, a neurogenic mutant. Homozygotes fail to form ventral, lateral and most of the cephalic epidermis. Central nervous system hypertrophied by recruitment of presumptive epidermal cells, but exhibiting considerable architectural normality. Most epidermal sense organs can be recognized in electron microscope preparations; chordotonal organs prevalent but with abnormal structures, perhaps owing to disrupted attachment.

(Lindsley and Zimm, 1992)

Loss of bib function approximately doubles the number of neuronal precursors and their progeny cells in the embryonic peripheral nervous system. Mosaic studies reveal a hypertrophy of sensory bristles in bib mutant patches in adult flies, in contrast to results of Dietrich and Campos-Ortega, 1984.

(Rao et al., 1992)

Mutant alleles cause hypertrophy in nau expressing cells, a 2-5 fold increase in nau expressing cells per cluster relative to wild type. The clusters enlarge so much that they merge to form superclusters across the midline.

(Corbin et al., 1991)

bib is needed for proper mesoderm differentiation prior to the onset of nau expression.

(Corbin et al., 1991)

Ecol\lacZ reporter gene constructs demonstrate that neurogenic loci are required to restrict the number of competent cells that will become sensory mother cells, SMCs.

(Goriely et al., 1991)

Mutations of mam, bib and neur in an heterozygous condition had no effect on the expression of N^Ax-59d or N^Ax-59b except when coupled in cis with N^fa-g. The neurogenic mutations suppress the wing venation phenotype of N.

(Portin and Rantanen, 1991)

Characterisation of bib genomic and complementary DNA suggests the bib product may mediate intercellular communication in a pathway separate from the one involving the products of other neurogenic genes.

(Rao et al., 1990)

bib mutants show no interaction with dx.

(Xu and Artavanis-Tsakonas, 1990)

Neural hyperplasia, caused by mutations in bib, can be prevented by the presence of another neurogenic mutation.

(Brand and Campos-Ortega, 1988)

Effects on central nervous system intermediate with respect to mutations at other neurogenic loci. Insensitive to changes in dosage of other neurogenic genes.

(de la Concha et al., 1988)

Supernumerary peripheral elements formed by recruitment of presumptive epidermal cells.

(Hartenstein and Campos-Ortega, 1986)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34330

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P23645

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

M9PB86

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0000180

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0000180

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

bib_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4722

Flygut - An atlas of the Drosophila adult midgut

FBgn0000180

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34330

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC010832

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4722

modMine - A data warehouse for the modENCODE project

FBgn0000180

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P23645

Linkouts

BioGRID - A database of protein and genetic interactions.

60424

DPiM - Drosophila Protein interaction map

FBgn0000180

DroID - A comprehensive database of gene and protein interactions.

FBgn0000180

DRSC - Results frm RNAi screens

FBgn0000180

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0000180

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG4722-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0000180

FlyMine - An integrated database for Drosophila genomics

FBgn0000180

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34330

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BIB

(Kourghi et al., 2018)

BIB_DROME

(Froger et al., 1998)

Bib

(Finn et al., 2015, Leiserson and Keshishian, 2011, del Alamo and Mlodzik, 2008, Li et al., 2008, Rand et al., 2008, Assa-Kunik et al., 2007, Kaufmann et al., 2005, Sun and Deng, 2005)

CG4722

(Tsubouchi et al., 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Molnar et al., 2006)

DmBIB

(Kourghi et al., 2017)

EP(2)2278

(Pena-Rangel et al., 2002)

bib

(Zhang et al., 2020, Hall et al., 2019, Wittes and Schüpbach, 2019, Schwarz et al., 2018, Yamakawa et al., 2018, Kojima, 2017, Kourghi et al., 2017, Córdoba et al., 2016, Córdoba and Estella, 2014, Guarner et al., 2014, Djiane et al., 2013, Lavagnino et al., 2013, Sen et al., 2013, Webber et al., 2013, Yousefian et al., 2013, Capilla et al., 2012, Tsubouchi et al., 2012, Cherbas et al., 2011, Goto et al., 2011, Jungreis et al., 2011, Pueyo and Couso, 2011, Herz et al., 2010, Kanwar and Fortini, 2010, Salzer et al., 2010, Tajiri et al., 2010, Carreira et al., 2009, Greenberg and Hatini, 2009, Krejcí et al., 2009, Liu et al., 2009, Tatsumi et al., 2009, Christensen et al., 2008.12.28, Mensch et al., 2008, Park et al., 2008, Christensen and Cook, 2007.3.22, Firth and Baker, 2007, Lecuyer et al., 2007, Magalhaes et al., 2007, Shirai et al., 2007, Christensen and Cook, 2006.8.30, Molnar et al., 2006, Sandmann et al., 2006, Galindo et al., 2005, Wang et al., 2004, Yanochko and Yool, 2004)

l(2)neo66

l(2)neo91

m-bib

(Burris et al., 1998)

Name Synonyms

Big Brain

(Kourghi et al., 2018)

Big brain

(Li et al., 2008, Assa-Kunik et al., 2007)

big brain

(Córdoba et al., 2016, Wieschaus and Nüsslein-Volhard, 2016, Wang et al., 2014, Tsubouchi et al., 2012, Salzer et al., 2010, Tajiri et al., 2010, Tatsumi et al., 2009, Park et al., 2008, Firth and Baker, 2007, Shirai et al., 2007, Beckstead et al., 2005, Galindo et al., 2005, Yanochko and Yool, 2004, Rao et al., 1992)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (211)

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