l(1)6P6, fs(1)107, fs(1)A107
Gene model reviewed during 5.52
Gene model reviewed during 5.55
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\brn using the Feature Mapper tool.
Northern analysis shows that brn is expressed in ovaries, in adult males and females, and throughout embryogenesis. In situ analysis of oogenesis shows that the expression of brn begins in germ cells in germarium region 2a, at the time when follicle cells first surround the nurse cell-oocyte complex. brn expression continues uniformly until oocyte stage S10A, when transcript levels in oocytes and nurse cells increase sharply.
GBrowse - Visual display of RNA-Seq signalsView Dmel\brn in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
grk plays a role in the development of follicular epithelium by cooperating with brn for the Egfr-dependent migration of the prefollicular cels around each nurse cell-oocyte complex. brn may help provide specificity to and/or facilitate the multiplicity of grk-Egfr functions during oogenesis.
Phenotypic analysis proposes that brn and egh, originating from the germline, collaborate with N on the apical surface of follicle cells to mediate germline-follicle cell adhesion. Wild type function of egh and brn is required for the formation of the follicular epithelium and maintenance of the apical-basal polarity, to inhibit follicle cell division, induction of dorsal follicle cell fates (but not specification of polar/stalk cell fates) and for concerted border cell-main body epithelium migration.
brn has a role in chorion formation and neurogenesis. Double mutant phenotypes with Egfr alleles suggest brn is required for dorsoventral patterning of the ovarian follicle. Mosaic experiments show that whereas the Egfr product is required in the follicle cells, the brn product is required in the germline. The brn/Egfr interaction is required for migration of prefollicular cells between each oocyte/nurse cell complex and for establishing continuous follicular epithelium around each oocyte/nurse cell complex. brn may be part of a germline signalling pathway differentially regulating successive Egfr-dependent follicle cell activities.