FB2022_02, released March 29, 2022

Gene: Dmel\by

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General Information
Symbol
Dmel\by
Species
D. melanogaster
Name
blistery
Annotation Symbol
CG9379
Feature Type
protein_coding_gene
FlyBase ID
FBgn0000244
Gene Model Status
Current
Stock Availability
18 publicly available
Gene Summary
blistery (by) encodes fly tensin, an adaptor protein that can bind integrins and actin and colocalizes with integrins in numerous tissues. Null alleles have a mild defect in integrin adhesion, as they are adult viable with loss of adhesion between the wing epithelia. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Also Known As

tensin, 3R-B, CT26639

Key Links
Genomic Location
Cytogenetic map
85D19-85D22
Sequence location
Recombination map
3-48
RefSeq locus
NT_033777 REGION:9528238..9533011
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables actin filament binding
inferred from direct assay
(Torgler et al., 2004)
enables cytoskeletal protein-membrane anchor activity
inferred from mutant phenotype
(Torgler et al., 2004)
enables phosphotyrosine residue binding
inferred from mutant phenotype
(Torgler et al., 2004)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (3 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in apposition of dorsal and ventral imaginal disc-derived wing surfaces
inferred from mutant phenotype
(Prout et al., 1997)
involved_in imaginal disc-derived wing morphogenesis
inferred from mutant phenotype
(Lee et al., 2003, Prout et al., 1997)
involved_in positive regulation of cell-cell adhesion mediated by integrin
inferred from mutant phenotype
(Torgler et al., 2004)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in imaginal disc-derived wing morphogenesis
non-traceable author statement
(Brown et al., 2000)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in focal adhesion
inferred from direct assay
(Delon and Brown, 2009)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in focal adhesion
inferred from biological aspect of ancestor with PANTHER:PTN002906772
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in focal adhesion
inferred from sequence or structural similarity
(Hynes and Zhao, 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
blistery (by) encodes fly tensin, an adaptor protein that can bind integrins and actin and colocalizes with integrins in numerous tissues. Null alleles have a mild defect in integrin adhesion, as they are adult viable with loss of adhesion between the wing epithelia. [Date last reviewed: 2019-03-07]
Phenotypic Description (Red Book; Lindsley and Zimm 1992)
by: blistery
Wings blistered in subterminal region; wing surface dusky and warped. Thorax humpy. RK1.
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\by for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VHC3)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
by-RA
FBtr0082119
NM_141644
2993
720
by-RC
FBtr0301093
NM_001170099
4774
720
by-RD
FBtr0302382
NM_001170098
4378
720
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
by-PA
FBpp0081597
79.5
720
9.20
NP_649901
AAF54393
by-PC
FBpp0290315
79.5
720
9.20
NP_001163570
ACZ94867
by-PD
FBpp0291577
79.5
720
9.20
NP_001163569
ACZ94866
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

720 aa isoforms: by-PA, by-PC, by-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\by using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
S2 cell-line
(Wheeler and Hynes, 2001)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in focal adhesion
inferred from direct assay
(Delon and Brown, 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\by in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 16 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 21 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of by
Transgenic constructs containing regulatory region of by
Aberrations (Deficiencies and Duplications) ( 7 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\TNS2
9 of 15
Yes
Yes
Hsap\TNS1
7 of 15
No
Yes
Hsap\TNS3
7 of 15
No
Yes
Hsap\TNS4
6 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Tns2
9 of 15
Yes
Yes
Mmus\Tns3
7 of 15
No
Yes
Mmus\Tns4
7 of 15
No
Yes
Mmus\Tns1
6 of 15
No
Yes
Rattus norvegicus (Norway rat) (4)
Rnor\Tns1
5 of 13
Yes
Yes
Rnor\Tns4
5 of 13
Yes
Yes
Rnor\Tns2
4 of 13
No
Yes
Rnor\Tns3
4 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\tns2
5 of 12
Yes
Yes
Xtro\tns1
3 of 12
No
Yes
Danio rerio (Zebrafish) (6)
Drer\tns2a
7 of 15
Yes
Yes
Drer\si:ch211-191a24.3
5 of 15
No
Yes
Drer\tns2b
5 of 15
No
Yes
Drer\tns1a
4 of 15
No
Yes
Drer\tns1b
4 of 15
No
Yes
Drer\tns3.2
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\tns-1
10 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Egfrap
2 of 10
PVRAP
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    TNS2; tensin 2
    9 of 15
    607717
        TNS1; tensin 1
        7 of 15
        600076
            TNS3; tensin 3
            7 of 15
            606825
                TNS4; tensin 4
                6 of 15
                608385
                    Functional Complementation Data
                    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                    Interactions
                    Summary of Physical Interactions
                    esyN Network Diagram
                    Show neighbor-neighbor interactions:
                    Select Layout:
                    Legend:
                    Protein
                    RNA
                    Selected Interactor(s)
                    Interactions Browser
                    View in Interactions Browser
                    Please see the Physical Interaction reports below for full details
                    protein-protein
                    Physical Interaction
                    Assay
                    References
                    by - Pvr
                    two hybrid
                    (Bianco et al., 2007)
                    Summary of Genetic Interactions
                    esyN Network Diagram
                    esyN Network Key:
                    Suppression
                    Enhancement
                    View in Interactions Browser
                    Please look at the allele data for full details of the genetic interactions
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    by
                    enhanceable
                    bsk
                    (Lee et al., 2003)
                    by
                    enhanceable
                    hep
                    (Lee et al., 2003)
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    if
                    enhanceable
                    by
                    (Lee et al., 2003)
                    External Data
                    Linkouts
                    BioGRID - A database of protein and genetic interactions.
                    DroID - A comprehensive database of gene and protein interactions.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Pathways
                    Signaling Pathways (FlyBase)
                    Metabolic Pathways
                    External Data
                    Linkouts
                    Genomic Location and Detailed Mapping Data
                    Chromosome (arm)
                    3R
                    Recombination map
                    3-48
                    Cytogenetic map
                    85D19-85D22
                    Sequence location
                    FlyBase Computed Cytological Location
                    Cytogenetic map
                    Evidence for location
                    85D19-85D22
                    Limits computationally determined from genome sequence between P{PZ}ps10615&P{PZ}Ras85D06677 and P{EP}CG9393EP3122
                    Experimentally Determined Cytological Location
                    Cytogenetic map
                    Notes
                    References
                    Experimentally Determined Recombination Data
                    Location

                    3-48

                    (FlyBase, 2016)

                    3-46.1

                    (Comeron et al., 2012)

                    3-

                    (Prout et al., 1997)

                    3-48.7

                    Left of (cM)
                    Right of (cM)
                    Notes

                    Located on 3R.

                    (Prout et al., 1997)
                    Stocks and Reagents
                    Stocks (18)
                    Genomic Clones (25)
                    cDNA Clones (170)
                     

                    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                    cDNA clones, fully sequenced
                    BDGP DGC clones
                    Other clones
                      Drosophila Genomics Resource Center cDNA clones

                      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                      cDNA Clones, End Sequenced (ESTs)
                      BDGP DGC clones
                      Other clones
                      RNAi and Array Information
                      Linkouts
                      DRSC - Results frm RNAi screens
                      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                      Antibody Information
                      Laboratory Generated Antibodies
                       
                      Commercially Available Antibodies
                       
                      Other Information
                      Relationship to Other Genes
                      Source for database identify of

                      Source for identity of: tensin CG9379

                      (Hynes and Zhao, 2000)
                      Source for database merge of

                      Source for merge of: by 3R-B

                      (Walsh and Brown, 1998)

                      Source for merge of: by tensin

                      (Lee et al., 2003)

                      Source for merge of: by BcDNA:RE65113

                      (Rana, 2004.7.2)
                      Additional comments
                      Other Comments

                      Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.

                      (Liu and Lehmann, 2008)

                      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

                      (Kiger et al., 2003)

                      Identification: Genetic screen for autosomal mutations that produce blisters in somatic wing clones. 1 allele has been isolated.

                      (Prout et al., 1997)
                      Origin and Etymology
                      Discoverer

                      Glass, Jan. 1933.

                      Etymology
                      Identification
                      External Crossreferences and Linkouts ( 52 )
                      Sequence Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                      InterPro - A database of protein families, domains and functional sites
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      MARRVEL_MODEL - MARRVEL (model organism gene)
                      modMine - A data warehouse for the modENCODE project
                      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                      FlyMine - An integrated database for Drosophila genomics
                      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                      MIST (protein-protein) - An integrated Molecular Interaction Database
                      Synonyms and Secondary IDs (8)
                      Datasets (8)
                      Study focus (0)
                      Experimental Role
                      Project
                      Project Type
                      Title
                      Study result (8)
                      Result
                      Result Type
                      Title
                      scRNAseq_2020_Cattenoz_NI_seq_clustering
                      Clustering analysis of hemocytes from non-infested third instar larvae
                      scRNAseq_2020_Cattenoz_WI_seq_clustering
                      Clustering analysis of hemocytes from wasp-infested third instar larvae
                      scRNAseq_2020_Cho_NI72LG_seq_clustering
                      Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
                      scRNAseq_2020_Cho_NI96LG_seq_clustering
                      Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
                      scRNAseq_2020_Cho_NI120LG_seq_clustering
                      Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
                      scRNAseq_2020_Cho_WI96LG_seq_clustering
                      Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
                      scRNAseq_2020_Cho_NI96HL_seq_clustering
                      Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
                      scRNAseq_2020_Cho_NI120HL_seq_clustering
                      Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
                      References (89)