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Gene Dmel\car

General Information
Symbol	Dmel\car	Species	D. melanogaster
Name	carnation	Annotation symbol	CG12230
Feature type	protein_coding_gene	FlyBase ID	FBgn0000257
Gene Model Status	Current	Stock availability	58 publicly available
Also Known As	Vps33, VPS33a
Genomic Location
Chromosome (arm)	X	Recombination map	1-62.5
Cytogenetic map	18D1-18D1	Sequence location	X:19,460,052..19,463,434 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene carnation is referred to in FlyBase by the symbol Dmel\car (CG12230, FBgn0000257). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: eye pigment granule organization; endosome to lysosome transport; regulation of protein ubiquitination; compound eye pigmentation; autophagic vacuole fusion; protein targeting to lysosome; determination of adult lifespan; endosome organization; ommochrome biosynthetic process. 17 alleles are reported. The phenotypes of these alleles are annotated with: Malpighian tubule; mesothoracic tergum; eye; late endosome; embryonic/larval brain; pigment cell. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Sec1-like protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult salivary gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1629509_s_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of car. Gene sequence location is X:19460052..19463434.
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	car: carnation Eye color dark ruby. Body shape and proportions seem rounded. With st, eye color is yellow-brown; with bw, brownish yellow to brown (Mainx, 1938, Z. Indukt. Abstamm. Vererbungsl. 75: 256-76). Malpighian tubes pale yellow in mature larva (Beadle, 1937, Genetics 22: 587-611) but hard to distinguish from wild type before third instar. Eye color autonomous in transplant into wild-type host (Beadle and Ephrussi, 1936, Genetics 21: 230). car dor is pupal lethal and shows reduced recovery in gynandromorphs with male parts car dor (Nash, 1971, DIS 47: 73). car lt also lethal, dies as larva when mother car;lt/+ or car/+;lt and as pupa when mother car/+;lt/+ (Nickla, 1977, Nature 268: 638-39); lethal focus domineering; fate maps to ventral nervous system (Nickla, Lilly, and McCarthy, 1980, Experientia 36: 402-05). Brain histology abnormal [McCarthy and Nickla, 1980, Experientia 36: 1361-63 (fig.)].
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Du et al., 2011, PLoS ONE 6(9): e24168
FB2012_01	References Williamson et al., 2010, J. Cell Biol. 189(5): 885--899 Sequence features HFA20316 BKN28988
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 18D1-18D1   Limits computationally determined from genome sequence between P{EP}EP1526&P{EP}PfrxEP1150 and P{EP}Tao-1EP1455
Experimentally Determined Cytological Location
Cytogenetic map Notes References 18D1-18D4   (determined by in situ hybridisation)   (Peter et al., 2002) 18D1-18D4   (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 18D1-18D2     (Lindsley and Grell, 1968)
Experimentally Determined Recombination Data
Location	1-62.5
Left of (cM)	sol (Fischbach and Lyly-Hunerberg, 1983) l(1)adl13 l(1)adl15 l(1)adl14 (Homyk et al., 1986)
Right of (cM)	l(1)adl8 l(1)adl9 l(1)adl7 l(1)adl6 l(1)adl10 l(1)adl12 l(1)adl11 l(1)adl3 (Homyk et al., 1986)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\car for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.40
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) car-RA FBtr0074728  NM_078686   3095   617 car-RB FBtr0074727  NM_167664   3173   617
Additional Transcript Data & Comments
Reported size (kB)	3.022 (longest cDNA) (Sevrioukov et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein car-PA   FBpp0074497   68.8   617   6.74     NP_523410   AAF48972 car-PB   FBpp0074496   68.8   617   6.74     NP_728266   AAN09503
Additional Polypeptide Data & Comments
Reported size (kDa)	617 (aa) (Sevrioukov et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sec1-like protein (IPR001619)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264748   AA817218   AA940832   AE014298 AAF48972   AE014298 AAN09503   AF133260 AAD38513   AI258414   AI389000   AI514335   AI519211   AJ426996   AW942637   AY069498 AAL39643   BF490882   BF492272   BI235432   BI236587   BI483836   CL526132   CO154770   CO155380   CO157081   CO278201   CO289355   CO299898   CO304833   CO321088   CO345293   EC070276   EC218019   EC233134   EC254010   EC261016   EC263864   GH945176   GH947058   GH948821   GH948921   GH949779   GH952654   GH953323   GH954200   GH955621   GH955697   GH955884   GH956680   GH957183   GH957633   GH957733   GH958304   GH958557   GH959786   GH960707   GH961054   GH963036   GH965483   GH965882   GH966728   GH967100   GH969899   GH969971   GH971649   GH971759   GH971971   GH972505   GH972979   GH973179   GH974916   GH974981   GH975440   GH976052   GH977677   GH978675   GH978711   GH979469   GH980010   GH980201   GH980321   GH982817
UniProtKB/Swiss-Prot	Q9Y1I2
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0000257    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0000257 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0000257 expression level Developmental Stage   Expression Level embryo 00-02hr    2415 embryo 02-04hr    1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day    1440 adult female 30day    1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2415) embryo 02-04hr  (1415) embryo 04-06hr  (1299) embryo 06-08hr  (988) embryo 08-10hr  (1099) embryo 10-12hr  (1212) embryo 12-14hr  (669) embryo 14-16hr  (687) embryo 16-18hr  (504) embryo 18-20hr  (501) embryo 20-22hr  (423) embryo 22-24hr  (382) larva L1  (537) larva L2  (473) larva L3 12hr old  (447) larva L3 puffstage 1-2  (356) larva L3 puffstage 3-6  (548) larva L3 puffstage 7-9  (581) white prepupae new  (562) white prepupae 12hr  (704) white prepupae 24hr  (717) pupae 2d postWPP  (545) pupae 3d postWPP  (336) pupae 4d postWPP  (764) adult male 01day  (530) adult male 05day  (621) adult male 30day  (576) adult female 01day  (1068) adult female 05day  (1440) adult female 30day  (1504) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2415) embryo 02-04hr  1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day  1440 adult female 30day  1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2415 embryo 02-04hr    1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day    1440 adult female 30day    1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2415 embryo 02-04hr    1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day    1440 adult female 30day    1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0000257 expression level Developmental Stage   Expression Level embryo 00-02hr    2415 embryo 02-04hr    1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day    1440 adult female 30day    1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2415) embryo 02-04hr  (1415) embryo 04-06hr  (1299) embryo 06-08hr  (988) embryo 08-10hr  (1099) embryo 10-12hr  (1212) embryo 12-14hr  (669) embryo 14-16hr  (687) embryo 16-18hr  (504) embryo 18-20hr  (501) embryo 20-22hr  (423) embryo 22-24hr  (382) larva L1  (537) larva L2  (473) larva L3 12hr old  (447) larva L3 puffstage 1-2  (356) larva L3 puffstage 3-6  (548) larva L3 puffstage 7-9  (581) white prepupae new  (562) white prepupae 12hr  (704) white prepupae 24hr  (717) pupae 2d postWPP  (545) pupae 3d postWPP  (336) pupae 4d postWPP  (764) adult male 01day  (530) adult male 05day  (621) adult male 30day  (576) adult female 01day  (1068) adult female 05day  (1440) adult female 30day  (1504) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  2415 embryo 02-04hr  1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day  1440 adult female 30day  1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2415 embryo 02-04hr    1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day    1440 adult female 30day    1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2415 embryo 02-04hr    1415 embryo 04-06hr    1299 embryo 06-08hr    988 embryo 08-10hr    1099 embryo 10-12hr    1212 embryo 12-14hr    669 embryo 14-16hr    687 embryo 16-18hr    504 embryo 18-20hr    501 embryo 20-22hr    423 embryo 22-24hr    382 larva L1    537 larva L2    473 larva L3 12hr old    447 larva L3 puffstage 1-2    356 larva L3 puffstage 3-6    548 larva L3 puffstage 7-9    581 white prepupae new    562 white prepupae 12hr    704 white prepupae 24hr    717 pupae 2d postWPP    545 pupae 3d postWPP    336 pupae 4d postWPP    764 adult male 01day    530 adult male 05day    621 adult male 30day    576 adult female 01day    1068 adult female 05day    1440 adult female 30day    1504 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0000257    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0000257 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult salivary gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0000257 expression level Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0000257 expression level Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    190.525 Larval Midgut    92.7 Larval Hindgut    99.4 Larval Malpighian Tubules    66.7 Larval Fat Body    58.3 Larval Salivary Gland    99.5 Larval Trachea    81.4 Larval Carcass    102.5 Adult Head    102.6 Adult Eye    98.375 Adult Brain    179.4 Adult Thoracic-Abdominal Ganglion    186.6 Adult Crop    81.1 Adult Midgut    56 Adult Hindgut    67.9 Adult Malpighian Tubules    54.1 Adult Fat Body    87.1 Adult Salivary Gland    112.8 Adult Heart    88.075 Adult VirginFemale Spermatheca    64.3 Adult InseminatedFemale Spermatheca    59.3 Adult Ovary    42.8 Adult Testis    11.1 Adult Male Accessory Gland    73.4 Adult Carcass    63.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    102.6   NA  Eye    98.375   NA  Brain    179.4   190.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    186.6   NA  Crop    81.1   92.7  Midgut    56   99.4  Hindgut    67.9   66.7  Malpighian Tubules    54.1   58.3  Fat Body    87.1   99.5  Salivary Gland    112.8   NA  Heart    88.075   81.4  Trachea    NA   NA  VirginFemale Spermatheca    64.3   NA  InseminatedFemale Spermatheca    59.3   NA  Ovary    42.8   NA  Testis    11.1   NA  Male Accessory Gland    73.4   102.5  Carcass    63.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000257 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12230-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for car Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pupal stage, with Scer\GAL4Scer\FRT.Act5C cardsRNA.Scer\UAS lethal | recessive | second instar larval stage carΔ146 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 carGD1397 semi-lethal | recessive carG0447 viable car1 carEY09656 Sterility Allele fertile carEY09656 Other Phenotypes Allele eye color defective car1 car2 car26-48 carunspecified eye color defective, with Scer\GAL4Scer\FRT.Act5C cardsRNA.Scer\UAS short lived | male car1 visible, with Scer\GAL4pnr-MD237 carGD1397 visible | recessive car1 car2 car26-48 visible | recessive | somatic clone carΔ146 wild-type carGMR.PS Phenotype manifest in Allele embryonic/larval brain car1 eye car1 late endosome car1 Malpighian tubule car1 mesothoracic tergum, with Scer\GAL4pnr-MD237 carGD1397 ommatidium | somatic clone carΔ146 pigment cell car1 car2 car26-48 carunspecified pigment cell | somatic clone carΔ146 rhabdomere | cell autonomous | somatic clone carΔ146 wing | somatic clone carΔ146
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of car Class Mutagen Stocks Known lesion car1 X ray 53 Yes carEY09656 P-element activity 1 -- carG0447 P-element activity 1 -- car26-48 sulfur mustard 0 -- car2 0 -- carunspecified 0 -- carΔ141 P-element activity 0 Yes carΔ146 loss of function allele P-element activity 0 Yes carΔ163 P-element activity 0 Yes carΔ33 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of car Class Mutagen Stocks Known lesion carGD1397 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes carHMS00972 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes car+t4.4 in vitro construct - genomic fragment 0 Yes cardsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes carGMR.PS in vitro construct - regulatory fusion 0 Yes carKK108988 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes carScer\UAS.cAa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)268 (Patterson, 1932) Df(1)BSC275 (Christensen and Cook, 2007.3.22) In(1)ClLbbDfR   In(1)y4Lsc4R   In(1)y4Lsc8R   Tp(1;2)26 (Pontecorvo, 1942)
Duplicated in	Dp(1;3)DC363 (Popodi, 2010.10.8) Dp(1;3)DC364 (Popodi et al., 2010-) Dp(1;f)3 (Schalet and Finnerty, 1968) Dp(1;f)52 (Schalet and Finnerty, 1968) Dp(1;f)100 (Morgan, 1938) In(1)bbDfLClR   In(1)bbDfLy4R   In(1)sc4Ly4R   In(1)sc8Ly4R   In(1)y3PLsc9R
Not disrupted in	Df(1)244 (Patterson, 1932) Df(1)267 (Patterson, 1932) Df(1)274 (Patterson, 1932) Df(1)D43L1 (Schalet and Finnerty, 1968) Df(1)DCB1-35c (Schalet and Finnerty, 1968) Df(1)JA27 (Craymer and Roy, 1980) Df(1)mal6 (Schalet and Finnerty, 1968) Df(1)mal8 (Schalet and Finnerty, 1968) Df(1)mal10 (Schalet and Finnerty, 1968) Df(1)mal13 (Schalet and Finnerty, 1968) Df(1)O4 (Oliver, 1937) In(1)ClLy4R
Not duplicated in	Dp(1;f)65 (Gethmann, 1967) Dp(1;f)65XC2 (Gethmann, 1967) Dp(1;f)sc7.2   Dp(1;Y)mal+   Ts(1Rt;3Lt)102
Partially disrupted in	Df(1)carΔ33 (Akbar et al., 2009) Df(1)carΔ141 (Akbar et al., 2009) Df(1)carΔ163 (Akbar et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1397} NA P{KK108988} NA P{TRiP.HMS00972} NA P{UAS-car.A} NA P{UAS-car.WIZ} NA characterization construct P{car+t4.4} NA P{GMR-car.S} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}carEY09656 NA insertion of enhancer trap P{lacW}carG0447 No P{lacW}l(1)G0041G0041 No
Gene Ontology: Function, Process & Cellular Component ( 18 unique terms )
Terms Based on Experimental Evidence ( 11 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with dor (assigned by UniProtKB) (Sevrioukov et al., 1999)
Biological Process
CV term Evidence References
autophagic vacuole fusion inferred from mutant phenotype (Akbar et al., 2009) compound eye pigmentation inferred from mutant phenotype (Falcon-Perez et al., 2007, Chi et al., 2010) determination of adult lifespan inferred from mutant phenotype (Simonsen et al., 2007) endosome organization inferred from mutant phenotype (Sriram et al., 2003) endosome to lysosome transport inferred from mutant phenotype (Akbar et al., 2009) eye pigment granule organization inferred from mutant phenotype (Akbar et al., 2009) inferred from mutant phenotype (assigned by UniProtKB) (Sevrioukov et al., 1999) ommochrome biosynthetic process inferred from mutant phenotype (Tearle, 1991) protein targeting to lysosome inferred from mutant phenotype (assigned by UniProtKB) (Sevrioukov et al., 1999) regulation of protein ubiquitination inferred from mutant phenotype (Simonsen et al., 2007)
Cellular Component
CV term Evidence References
lysosome inferred from direct assay (assigned by UniProtKB) (Sevrioukov et al., 1999)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
SNARE binding inferred from sequence or structural similarity (Littleton, 2000)
Biological Process
CV term Evidence References
compound eye pigmentation traceable author statement (Toonen and Verhage, 2003) endosome to lysosome transport traceable author statement (Toonen and Verhage, 2003, Luzio et al., 2000) intracellular transport traceable author statement (Narayanan and Ramaswami, 2001) lysosome organization non-traceable author statement (Kramer, 1999.3.5) vesicle docking involved in exocytosis inferred from electronic annotation with InterPro:IPR001619 (FlyBase Curators et al., 2004-) vesicle-mediated transport inferred from sequence or structural similarity (Littleton, 2000)
Cellular Component
CV term Evidence References
cytosol traceable author statement (Toonen and Verhage, 2003) membrane traceable author statement (Toonen and Verhage, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions car allele Gene References car1 dor (Sriram et al., 2003) lt (Nickla et al., 1980, Warner et al., 1998) carunspecified w (Lloyd et al., 2002) unspecified bchs (Simonsen et al., 2007) g (Lindsley and Grell, 1968)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59247 DroID - A comprehensive database of gene and protein interactions. • FBgn0000257 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32947
Orthologs
Genome-wide drosophilid orthologs	Dana\GF20198 Dere\GG19231 Dgri\GH12312 Dmoj\GI21456 Dpse\GA11493 Dsec\GM22966 Dsim\GD17447 Dvir\GJ16477 Dwil\GK10350 Dyak\GE15849
Curated drosophilid orthologs	Dpse\GA11493
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL000214-PA Aedes aegypti (mosquito) AGAP003203-PA Anopheles gambiae (mosquito) XP_395353.1 Apis mellifera (honey bee) XP_001944765.1 Acyrthosiphon pisum (pea aphid) At3g54860.1 Arabidopsis thaliana (wall cress) 24481 Batrachochytrium dendrobatidis (chytrid fungus) 289566 Branchiostoma floridae (lancelet) BGIBMGA008046-PA Bombyx mori (silk worm) ENSBTAP00000005508 Bos taurus (bovine) CN28798 Caenorhabditis brenneri (small nematode) CBP18368 Caenorhabditis briggsae (nematode) CE30636 Caenorhabditis elegans (worm) ENSCAFP00000011731 Canis familiaris (dog) Chro.60276 Cryptosporidium hominis (obligate parasite) ENSCINP00000014583 Ciona intestinalis (sea squirt) JA03051 Caenorhabditis japonica (nematode) cn05179 Cryptococcus neoformans (yeast-like fungus) cgd6_2370 Cryptosporidium parvum (protozoan parasite) CPIJ013822 Culex pipens (mosquito) ENSCPOP00000008165 Cavia porcellus (guinea pig) 154542 Chlamidomonas reinhardtii (green alga) RP32785 Caenorhabditis remanei (nematode) ENSCSAVP00000015786 Ciona savignyi (sedentary tunicate) 101629 Capitella spl (polychaete worm) DDB0234138 Dictyostelium discoideum (amoeba) DEHA2E10604g Debaryomyces Hansenii (yeast) 303678 Daphnia pulex (daphnia) ENSDARP00000013152 Danio rerio (zebra fish) ENSDARP00000088013 Danio rerio (zebra fish) ENSECAP00000019260 Equus caballus (horse) ENSGACP00000014974 Gymnopilus aculeatus (mushroom) ENSGALP00000007052 Gallus gallus (chicken) GL50803_13528 Giardia lamblia (flagellated protozoan) 86965 Hypsipyla robusta (cedar shoot borer) ENSP00000267199 Homo sapiens (human) ISCW000790-PA Ixodes scapularis (deer tick) KLLA0B03267g Kluyveromyces lactis (yeast) 209143 Lottia gigantea (owl limpet) LmjF25.2180 Leishmania major (virus) 32472 Monocosta brevicollis (choanoflagellate) ENSMODP00000015299 Monodelphis domestica (gray opossum) ENSMMUP00000023702 Macaca mulatta (rhesus monkey) ENSMUSP00000031388 Mus musculus (house mouse) 96025 Nematostella vectensis (starlet anemone) XP_001603366.1 Nasonia vitripennis (wasp) ENSOANP00000009920 Ornithorynchus anatinus (platypus) ENSORLP00000015092 Oryzias latipes (japanese killifish) LOC_Os04g14654.1 Oryza sativa (asian rice) 20619 Ostreococcus tauri (green alga) PFI1700c Plasmodium falciparum (protozoal parasite) PHUM005681-PA Pediculus humanus (human body louse) PP27928 Pristionchus pacificus (nematode) 105112 Physcomitrella patens (moss) ENSPPYP00000005771 Pongo pygmaeus (orangutan) 84905 Phytophthora ramorum (sudden oak death pathogen) 143930 Phytophthora sojae (root rot pathogen) 563704 Populus trichocarpa (black cottonwood) ENSPTRP00000009469 Pan troglodytes (chimpanzee) PVX_086960 Plasmodium vivax (protozoal parasite) ENSRNOP00000011458 Rattus norvegicus (norway rat) 5053198 Sorghum bicolor (sorghum) YLR396C Saccharomyces cerevisiae (budding yeast) Smp_069670 Schistosoma mansoni (trematode parasite) SPBC1703.15c Schizosaccharomyces pombe (fission yeast) XP_798265.1 Strongylocentrotus purpuratus (purple sea urchin) 57259 Trichoplax adhaerens (metazoa) TA10760 Theileria annulata (apicomplexan parasite) XP_968688.1 Tribolium castaneum (red flour beetle) Tc00.1047053508479.290 Trypanosoma cruzi (parasitic trypanosome) TP04_0639 Theileria parva (parasitic protozoan) ENSTRUP00000001009 Takifugu rubripes (pufferfish Takifugu) 3836.m02291 Tetrahymena thermophila (ciliate protozoa) 87912.m00147 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000057529 Xenopus tropicalis (frog) YALI0F04125g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14182 NV16292 LH25249 ISCW000790 FBgn0233412 FBgn0212365 FBgn0203661 FBgn0189001 FBgn0177835 JGI_V11_329944 JGI_V11_303678 FBgn0071544 FBgn0152142 FBgn0152141 FBgn0144186 FBgn0119791 FBgn0111437 FBgn0097205 CPIJ013822 BGIBMGA008046 ACYPI004826 GB15752 AGAP003203 AAEL000214 PHUM608030 GLEAN_06102
Stocks & Reagents
Stocks Listed in FlyBase ( 58 )
Bloomington	19 car1 20 car1 su(f)8 579 Df(1)JF5, f1 car1/FM7a 12257 w67c23 P{lacW}carG0447 788 In(1)rst3, y1 car1 33 ec1 ct6 s1 car1/FM6
Kyoto	105680 car1 101065 car1 su(f)8 101194 v1 f3N car1 105990 Df(1)JF5, f1 car1/FM7a
VDRC	v4548 w1118; P{GD1397}v4548 v4547 w1118; P{GD1397}v4547/CyO
	More stocks available...
Genomic Clones ( 2 )
	BACR10M08 BACR27L16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 72 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD22396
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT27510 AT29179 GH20069 LD08438 LD23088 LD38724 LD40135 LP01613 RE31274 RE32658 RE66786
Other clones	17347 214541.51 524104 524794 CK01.42A.A6 CK01.90A.C6 EK070843 EK129323 EK176856 EK246504 EK290304 EN04753 EN05938 EN09909 EP21750 RP004029981 RP004037229 RP004051525 RP004054247 RP004064090 RP004073905 RP004088975 RP004092115 RP004093900 RP004117936 RP004131770 RP004140561 RP004179874 RP004190681 RP004193174 RP004213683 RP004216257 RP004234475 RP004242729 RP004242882 RP004243814 RP004250493 RP004256447 RP004257351 RP004283230 RP004329059 RP004342562 RP004352615 RP004353796 RP004386030 RP004389074 RP004397257 RP004404540 RP004407066 RP004408984 RP004432869 RP004435033 RP004473227 RP004476244 RP004486460 RP004491377 RP004519059 RP004538414 RP004543880 RP004553140
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0000257 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32947
Antibody Information
	polyclonal (Sevrioukov et al., 1999)
Other Information
Discoverer
	Patterson, 20th March 1928.
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: car l(1)G0447 (Peter et al., 2002) Source for merge of: car Vps33 (Sevrioukov et al., 1999)
Additional comments	"l(1)G0041" may affect "car". (Peter et al., 2002)
Other Comments
	car is required for endocytic trafficking to lysosomes. car is required for the biogenesis of pigment granules. (Akbar et al., 2009) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dor is part of a protein complex which includes car. (Sevrioukov et al., 1999) Lesions in car reduce eye and ocelli pigmentation and cause excretion of pigment from the tubules. (Tearle, 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078686 NM_167664 RefSeq (Proteins) • NP_523410 NP_728266 DDBJ / EMBL / GenBank • AA264748 AA817218 AAD38513 AAF48972 AAL39643 AAN09503 AF133260 AJ426996 AW942637 AY069498 CL526132 Entrez Gene - A searchable database of RefSeq genes. • 32947 UniProtKB/Swiss-Prot • Q9Y1I2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sec1-like protein (IPR001619)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59247 DroID - A comprehensive database of gene and protein interactions. • FBgn0000257 DRSC - Results from RNAi screens. • FBgn0000257 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0000257 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12230-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0000257 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32947 InParanoid A subset of ortholog calls from InParanoid. • AAEL000214-PA Aedes aegypti (mosquito) AGAP003203-PA Anopheles gambiae (mosquito) XP_395353.1 Apis mellifera (honey bee) XP_001944765.1 Acyrthosiphon pisum (pea aphid) At3g54860.1 Arabidopsis thaliana (wall cress) 24481 Batrachochytrium dendrobatidis (chytrid fungus) 289566 Branchiostoma floridae (lancelet) BGIBMGA008046-PA Bombyx mori (silk worm) ENSBTAP00000005508 Bos taurus (bovine) CN28798 Caenorhabditis brenneri (small nematode) CBP18368 Caenorhabditis briggsae (nematode) CE30636 Caenorhabditis elegans (worm) ENSCAFP00000011731 Canis familiaris (dog) Chro.60276 Cryptosporidium hominis (obligate parasite) ENSCINP00000014583 Ciona intestinalis (sea squirt) JA03051 Caenorhabditis japonica (nematode) cn05179 Cryptococcus neoformans (yeast-like fungus) cgd6_2370 Cryptosporidium parvum (protozoan parasite) CPIJ013822 Culex pipens (mosquito) ENSCPOP00000008165 Cavia porcellus (guinea pig) 154542 Chlamidomonas reinhardtii (green alga) RP32785 Caenorhabditis remanei (nematode) ENSCSAVP00000015786 Ciona savignyi (sedentary tunicate) 101629 Capitella spl (polychaete worm) DDB0234138 Dictyostelium discoideum (amoeba) DEHA2E10604g Debaryomyces Hansenii (yeast) 303678 Daphnia pulex (daphnia) ENSDARP00000013152 Danio rerio (zebra fish) ENSDARP00000088013 Danio rerio (zebra fish) ENSECAP00000019260 Equus caballus (horse) ENSGACP00000014974 Gymnopilus aculeatus (mushroom) ENSGALP00000007052 Gallus gallus (chicken) GL50803_13528 Giardia lamblia (flagellated protozoan) 86965 Hypsipyla robusta (cedar shoot borer) ENSP00000267199 Homo sapiens (human) ISCW000790-PA Ixodes scapularis (deer tick) KLLA0B03267g Kluyveromyces lactis (yeast) 209143 Lottia gigantea (owl limpet) LmjF25.2180 Leishmania major (virus) 32472 Monocosta brevicollis (choanoflagellate) ENSMODP00000015299 Monodelphis domestica (gray opossum) ENSMMUP00000023702 Macaca mulatta (rhesus monkey) ENSMUSP00000031388 Mus musculus (house mouse) 96025 Nematostella vectensis (starlet anemone) XP_001603366.1 Nasonia vitripennis (wasp) ENSOANP00000009920 Ornithorynchus anatinus (platypus) ENSORLP00000015092 Oryzias latipes (japanese killifish) LOC_Os04g14654.1 Oryza sativa (asian rice) 20619 Ostreococcus tauri (green alga) PFI1700c Plasmodium falciparum (protozoal parasite) PHUM005681-PA Pediculus humanus (human body louse) PP27928 Pristionchus pacificus (nematode) 105112 Physcomitrella patens (moss) ENSPPYP00000005771 Pongo pygmaeus (orangutan) 84905 Phytophthora ramorum (sudden oak death pathogen) 143930 Phytophthora sojae (root rot pathogen) 563704 Populus trichocarpa (black cottonwood) ENSPTRP00000009469 Pan troglodytes (chimpanzee) PVX_086960 Plasmodium vivax (protozoal parasite) ENSRNOP00000011458 Rattus norvegicus (norway rat) 5053198 Sorghum bicolor (sorghum) YLR396C Saccharomyces cerevisiae (budding yeast) Smp_069670 Schistosoma mansoni (trematode parasite) SPBC1703.15c Schizosaccharomyces pombe (fission yeast) XP_798265.1 Strongylocentrotus purpuratus (purple sea urchin) 57259 Trichoplax adhaerens (metazoa) TA10760 Theileria annulata (apicomplexan parasite) XP_968688.1 Tribolium castaneum (red flour beetle) Tc00.1047053508479.290 Trypanosoma cruzi (parasitic trypanosome) TP04_0639 Theileria parva (parasitic protozoan) ENSTRUP00000001009 Takifugu rubripes (pufferfish Takifugu) 3836.m02291 Tetrahymena thermophila (ciliate protozoa) 87912.m00147 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000057529 Xenopus tropicalis (frog) YALI0F04125g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32947 modMine - Data generated by the modENCODE project. • FBgn0000257 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14182 NV16292 LH25249 ISCW000790 FBgn0233412 FBgn0212365 FBgn0203661 FBgn0189001 FBgn0177835 JGI_V11_329944 JGI_V11_303678 FBgn0071544 FBgn0152142 FBgn0152141 FBgn0144186 FBgn0119791 FBgn0111437 FBgn0097205 CPIJ013822 BGIBMGA008046 ACYPI004826 GB15752 AGAP003203 AAEL000214 PHUM608030 GLEAN_06102
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	car (Falcon-Perez et al., 2007, Pulipparacharuvil et al., 2005, Akbar et al., 2007, Christensen and Cook, 2007.3.22, Du et al., 2011, Wilkin et al., 2008, Chinchore et al., 2009, Straub et al., 2008, Anaka et al., 2008, Soukup et al., 2009, Akbar et al., 2009, Popodi, 2010.10.8, Chi et al., 2010, Popodi et al., 2010-, Yonamine et al., 2011) Car (Kim et al., 2010, Williamson et al., 2010) carn (Simonsen et al., 2007) CG12230 (Dorer et al., 2006, Ni et al., 2010.12.1, Keleman et al., 2009.8.5) Dm-Vps33 (Sevrioukov et al., 1999) l(1)G0447 (Schaefer et al., 1999.12.2, ) Vps33 (Littleton, 2000, Kramer, 1999.3.5, Kim et al., 2010) Vps33/carnation (Toonen and Verhage, 2003) VPS33a (Dorer et al., 2006) Vps33a (Akbar et al., 2009) Vps33A (Kim et al., 2010) Vps33p (Littleton, 2000)
Name Synonym	carnation (Falcon-Perez et al., 2007, Du et al., 2011, Simonsen et al., 2007, Akbar et al., 2008, Wilkin et al., 2008, Pulipparacharuvil et al., 2005, Chinchore et al., 2009, Akbar et al., 2009, Anaka et al., 2008, Chi et al., 2010, Gissen et al., 2005) Vacuolar protein sorting protein 33
Secondary FlyBase IDs
	FBgn0027357 FBgn0040160
References ( 89 )
Generate a list of	All references relating to this gene ( 89 )
List References by type	Research paper  ( 53 ) Supplementary material  ( 4 ) Review  ( 9 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 6 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Book  ( 2 ) Computer file  ( 2 ) Curated genome annotation  ( 1 ) Stock list  ( 1 )
Recent research papers ( 5 )
	Du et al., 2011, PLoS ONE 6(9): e24168 In Vivo RNAi Screen Reveals Neddylation Genes as Novel Regulators of Hedgehog Signaling. [FBrf0215574] Yonamine et al., 2011, J. Cell Biol. 192(4): 557--567 Sphingosine kinases and their metabolites modulate endolysosomal trafficking in photoreceptors. [FBrf0213095] Chi et al., 2010, J. Biol. Chem. 285(28): 21817--21823 Disruption of lysosome function promotes tumor growth and metastasis in Drosophila. [FBrf0211186] Kim et al., 2010, J. Cell Biol. 188(5): 717--734 The novel endosomal membrane protein Ema interacts with the class C Vps-HOPS complex to promote endosomal maturation. [FBrf0210177] Williamson et al., 2010, J. Cell Biol. 189(5): 885--899 A dual function of V0-ATPase a1 provides an endolysosomal degradation mechanism in Drosophila melanogaster photoreceptors. [FBrf0210877] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...